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Visual screening for localized RNAs in yeast revealed novel RNAs at the bud-tip.

Abstract：

:Several RNAs, including rRNAs, snRNAs, snoRNAs, and some mRNAs, are known to be localized at specific sites in a cell. Although methods have been established to visualize RNAs in a living cell, no large-scale visual screening of localized RNAs has been performed. In this study, we constructed a genomic library in which random genomic fragments were inserted downstream of U1A-tag sequences under a GAL1 promoter. In a living yeast cell, transcribed U1A-tagged RNAs were visualized by U1A-GFP that binds the RNA sequence of the U1A-tag. In this screening, many RNAs showed nuclear signals. Since the nuclear signals of some RNAs were not seen when the U1A-tag was connected to the 3' ends of the RNAs, it is suggested that their nuclear signals correspond to nascent transcripts on GAL1 promoter plasmids. Using this screening method, we successfully identified two novel localized mRNAs, CSR2 and DAL81, which showed bud-tip localization.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Andoh T,Oshiro Y,Hayashi S,Takeo H,Tani T

doi

10.1016/j.bbrc.2006.10.139

subject

Has Abstract

pub_date

2006-12-29 00:00:00

pages

999-1004

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)02409-0

journal_volume

351

pub_type

杂志文章

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