Purification and analysis of the structure of alpha-galactosidase from Escherichia coli.

abstract：:Free fatty acids (FFAs) act as extracellular signaling molecules through binding to G-protein-coupled FFA receptors (FFARs). GPR120 and GPR40 are identified as FFARs for medium- and long-chain fatty acids. In the present study, we investigated roles of GPR120 and GPR40 in cellular functions of pancreatic cancer PANC-1...

journal_title：Biochemical and biophysical research communications

authors： Fukushima K,Yamasaki E,Ishii S,Tomimatsu A,Takahashi K,Hirane M,Fukushima N,Honoki K,Tsujiuchi T

更新日期：2015-09-25 00:00:00

abstract：:HMGB1 is a conserved chromosomal protein composed of two DNA-binding domains and an acidic C-terminal tail. There were evidences suggesting that the C-terminal tail contributed to the DNA binding specificity of the N-terminal DNA-binding domains. However, the mechanism underlining this observation is largely unknown. ...

journal_title：Biochemical and biophysical research communications

authors： Wang Q,Zeng M,Wang W,Tang J

更新日期：2007-08-17 00:00:00

abstract：:VDR regulates gene expression in a ligand-dependent way by binding to cognate enhancer elements of target gene promoters. The ligand-dependent activation function, AF-2, of VDR is thought to require transcriptional co-activators/co-repressors together with basal transcriptional machinery. Using a yeast two hybrid syst...

journal_title：Biochemical and biophysical research communications

authors： Imai T,Matsuda K,Shimojima T,Hashimoto T,Masuhiro Y,Kitamoto T,Sugita A,Suzuki K,Matsumoto H,Masushige S,Nogi Y,Muramatsu M,Handa H,Kato S

更新日期：1997-04-28 00:00:00

abstract：:The newly identified drug transporter MRP is functionally linked to a multiple drug resistance independent from P-glycoprotein. Resistance modifiers for this type of MDR are rare at present. We analyzed the modulating effect of the highly selective bisindolylmaleimide PKC inhibitor GF 109203X on the MRP overexpressing...

journal_title：Biochemical and biophysical research communications

authors： Gekeler V,Boer R,Ise W,Sanders KH,Schächtele C,Beck J

更新日期：1995-01-05 00:00:00

abstract：:We describe the genomic organization and the functional promoter of the monocyte specific gene Dif-2, the human homologue to genes in mouse (gly96) and rat (PRG1), that is downregulated during cell differentiation. The Dif-2 gene consists of two exons and a single intron of 112 bp in length. RNase protection assay ind...

journal_title：Biochemical and biophysical research communications

authors： Pietzsch A,Büchler C,Schmitz G

更新日期：1998-04-28 00:00:00

abstract：:Matrix metalloproteinase-9 (MMP-9) plays a major role in the pathogenesis of atherosclerosis and restenosis by regulating both migration and proliferation of vascular smooth muscle cells (VSMC) after an arterial injury. In this study, we examined the inhibitory effect of three major flavonoids in Scutellariae Radix, b...

journal_title：Biochemical and biophysical research communications

authors： Lee SO,Jeong YJ,Yu MH,Lee JW,Hwangbo MH,Kim CH,Lee IS

更新日期：2006-12-08 00:00:00

abstract：:The recently detected Rieske iron-sulfur center in the membrane of the thermoacidophilic archaeon Sulfolobus acidocaldarius (Anemüller et al., 1993, FEBS Lett. 318, 61-64) was further characterized by EPR spectroscopy, coupled to redox-potentiometry and functional studies. The reduction potential is pH-dependent above...

journal_title：Biochemical and biophysical research communications

authors： Anemüller S,Schmidt CL,Schäfer G,Bill E,Trautwein AX,Teixeira M

更新日期：1994-07-15 00:00:00

abstract：:The gene for acetyl-CoA carboxylase, the rate-limiting enzyme in the biosynthesis of long-chain fatty acids, contains two promoters; promoter I (PI) and promoter II (PII) which are responsible for generation of class I and class II ACC mRNAs, respectively. Class I ACC mRNAs are present in adipose tissue, but only a tr...

journal_title：Biochemical and biophysical research communications

authors： Ekhterae D,Tae HJ,Daniel S,Moller DE,Kim KH

更新日期：1996-10-14 00:00:00

abstract：:The Impact is an evolutionarily conserved gene subjected to genomic imprinting in mouse but not in human. A characteristic tandem repeat similar to those found in many other imprinted genes and an elevated expression level, both observed only for the mouse gene, are implicated in the evolution of imprinting, to which ...

journal_title：Biochemical and biophysical research communications

authors： Okamura K,Sakaki Y,Ito T

更新日期：2005-04-15 00:00:00

abstract：:The rSK3 gene encodes a small conductance Ca(2+)-activated K(+) channel that is transcriptionally regulated by estrogen. To examine determinants of rSK3 gene expression, the CAP site was defined and the promoter was identified. A 33 bp sequence adjacent to the promoter was shown to act as an enhancer in L6 cells that ...

journal_title：Biochemical and biophysical research communications

authors： Jacobson D,Pribnow D,Herson PS,Maylie J,Adelman JP

更新日期：2003-04-04 00:00:00

abstract：:Six forms of cytochrome P-450 in the mitochondria of larvae from Musca domestica were isolated by solubilization with CHAPS followed by ammonium sulfate fractionation and HPLC on an anion-exchange column. Forms 1, 2, 3, 5, and 6 catalyzed the formation of 20-hydroxy-ecdysone from ecdysone in the presence of NADPH and ...

journal_title：Biochemical and biophysical research communications

authors： Srivatsan J,Weirich M,Agosin M

更新日期：1990-02-14 00:00:00

abstract：:Dexamethasone produced an increased activity of stearoyl-CoA desaturase through the enhancement of delta 9-terminal component activity, and a corresponding decrease of oleoyl-CoA desaturase activity via the reduced activity of delta 12-terminal component in Tetrahymena microsomes. However, the content of cytochrome b5...

journal_title：Biochemical and biophysical research communications

authors： Umeki S,Nozawa Y

更新日期：1984-02-29 00:00:00

abstract：:NF-kappaB plays an important role in oncogenesis. Recently, we have demonstrated that loss of p53 function enhances DNA binding and transcriptional activities of NF-kappaB via IKKalpha and IKKbeta, and that glycolysis, activated by NF-kappaB, has an integral role in oncogene-induced cell transformation. Here, we show ...

journal_title：Biochemical and biophysical research communications

authors： Kawauchi K,Araki K,Tobiume K,Tanaka N

更新日期：2008-07-18 00:00:00

abstract：:Alzheimer disease (AD) is a heterogeneous disorder with a variety of molecular pathologies converging predominantly on abnormal amyloid deposition particularly in the brain. beta-Amyloid aggregation into senile plaques is one of the pathological hallmarks of AD. beta-Amyloid is generated by a proteolytic cleavage of a...

journal_title：Biochemical and biophysical research communications

authors： Elbaum D,Brzyska M,Bacia A,Alkon DL

更新日期：2000-01-27 00:00:00

abstract：:The question of how eukaryotic cells assemble their mitochondria was long considered to be inaccessible to biochemical investigation. This attitude changed about fifty years ago when the powerful tools of yeast genetics, electron microscopy and molecular biology were brought to bear on this problem. The rising interes...

journal_title：Biochemical and biophysical research communications

authors： Schatz G

更新日期：2013-05-10 00:00:00

abstract：:We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence reveal...

journal_title：Biochemical and biophysical research communications

authors： Okazaki K,Watanabe M,Ando Y,Hagiwara M,Terasawa M,Hidaka H

更新日期：1992-05-29 00:00:00

abstract：:Glucocorticoids cause the delayed wound healing by suppressing inflammation that is required for wound healing process. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) play an important role for wound healing by their cytokine productions including stromal derived factor 1 (SDF-1). However, it has not been cle...

journal_title：Biochemical and biophysical research communications

authors： Kato T,Khanh VC,Sato K,Takeuchi K,Carolina E,Yamashita T,Sugaya H,Yoshioka T,Mishima H,Ohneda O

更新日期：2017-11-18 00:00:00

abstract：:The development of central nervous system (CNS) neuronal networks involves processes including neuroblast migration, axonal pathfinding, and synaptogenesis. To evaluate the role of the axonal guidance molecule DSCAM in CNS connectivity, we generated a lacZ reporter construct, Pr1.8-betagal, containing a 1.8kb fragment...

journal_title：Biochemical and biophysical research communications

authors： Barlow GM,Lyons GE,Richardson JA,Sarnat HB,Korenberg JR

更新日期：2002-11-22 00:00:00

abstract：:In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. The ROCK-specific inhibitor, Y-27632, did not alter basal amylase secretion, whereas it potentiated CCK-...

journal_title：Biochemical and biophysical research communications

authors： Kusama K,Nozu F,Awai T,Tanaka S,Honma I,Tsunoda Y,Mitamura K

更新日期：2003-05-30 00:00:00

abstract：:Sterol carrier protein X (SCPx) is a peroxisomal protein with both lipid transfer and thiolase activity. Treatment of mouse adrenal Y1 cells with cAMP for 24h caused a significant induction of SCPx mRNA levels. Reporter gene studies demonstrated that treatment with cAMP and SF-1 was able to activate the SCPx promoter....

journal_title：Biochemical and biophysical research communications

authors： Lopez D,Niesen M,Bedi M,Hale D,McLean MP

更新日期：2007-06-01 00:00:00

abstract：:Apoptosis is a well defined physiological process characterised by many specific features including DNA fragmentation and protease activation. Cell membrane-associated changes such as the altered exposure of phosphatidylserine and glycosylation patterns have also been described. We investigate here the change in expos...

journal_title：Biochemical and biophysical research communications

authors： Russell L,Waring P,Beaver JP

更新日期：1998-09-18 00:00:00

abstract：:Microcalorimetric titrations have been used to study the binding of Ca2+ to cardiac troponin C. Measurements were made both in the presence and in the absence of Mg2+, and at temperatures of 5 degrees, 15 degrees and 25 degrees C. Changes in enthalpy, entropy and heat capacity of troponin C associated with Ca binding ...

journal_title：Biochemical and biophysical research communications

authors： Kometani K,Yamada K

更新日期：1983-07-18 00:00:00

abstract：:The human promyelocytic leukemia cell line HL-60 can be differentiated to cells resembling either neutrophils or mononuclear phagocytes by a diverse group of stimuli. However, the underlying mechanisms remain unknown. We report that 1-0-hexadecyl-2-acetyl-sn-glycerol inhibits the growth of HL-60 cells and induces diff...

journal_title：Biochemical and biophysical research communications

authors： McNamara MJ,Schmitt JD,Wykle RL,Daniel LW

更新日期：1984-07-31 00:00:00

abstract：:Histone deacetylase inhibitors (HDACIs) are promising anti-tumor agents that selectively induce cell cycle arrest, differentiation and/or apoptosis of tumor cells. Fundamentally, HDACIs are proposed to function by activating the transcription of genes, including the potent cyclin dependent kinase inhibitor p21(WAF1). ...

journal_title：Biochemical and biophysical research communications

authors： Hirsch CL,Ellis DJ,Bonham K

更新日期：2010-11-26 00:00:00

abstract：:The effect of cellular stress factors, the hyperthermia and the acidification of culture medium, on mechano-growth factor (MGF) synthesis by murine myoblasts in culture was investigated. Hyperthermia was shown to stimulate MGF expression both in primary myoblasts and in differentiated multinuclear myotubes. The induct...

journal_title：Biochemical and biophysical research communications

authors： Kravchenko IV,Furalyov VA,Popov VO

更新日期：2008-10-17 00:00:00

abstract：:One of the earliest events following stimulation of human platelets with thrombin is a rise in the cytosolic pH, pHi, mediated by Na+/H+ exchange, and an increase in the cytosolic free Ca2+ concentration, [Ca2+]i. In the present study we investigated whether an increase in pHi alone, induced by the Na+/H+ ionophore mo...

journal_title：Biochemical and biophysical research communications

authors： Siffert W,Akkerman JW

更新日期：1989-06-30 00:00:00

abstract：:Capsaicin has been reported to regulate blood glucose levels and to ameliorate insulin resistance in obese mice. This study demonstrates that capsaicin increases glucose uptake directly by activating AMP-activated protein kinase (AMPK) in C2C12 muscle cells, which manifested as an attenuation of glucose uptake when co...

journal_title：Biochemical and biophysical research communications

authors： Kim SH,Hwang JT,Park HS,Kwon DY,Kim MS

更新日期：2013-09-13 00:00:00

abstract：:A complementary DNA clone encoding serum amyloid A protein has been isolated from an acute rabbit liver cDNA library. Complete nucleotide sequence analysis reveals that the cloned gene contains a 24 bases 5' untranslated region, 369 bases coding region and a 106 bases 3' untranslated region. Primer extension analysis ...

journal_title：Biochemical and biophysical research communications

authors： Ray BK,Ray A

更新日期：1991-07-15 00:00:00

abstract：:The substrate specificities of monoamine oxidase (MAO) A isolated from human placenta and of human liver expressed in yeast have been compared in homogeneous preparations with respect to Vmax and Km values for natural and synthetic substrates and Ki values for competitive inhibitors. MAO A from these two sources is kn...

journal_title：Biochemical and biophysical research communications

authors： Tan AK,Weyler W,Salach JI,Singer TP

更新日期：1991-12-31 00:00:00

abstract：:Chikungunya is a severe disease that results from infection with the chikungunya virus (CHIKV), an arbovirus. Thus, we (1) explored a new approach to combining previously researched drugs that have shown the potential to inhibit CHIKV infection; and (2) demonstrated the antiviral effects of (-)-Epigallocatechin-3-gall...

journal_title：Biochemical and biophysical research communications

authors： Lu JW,Hsieh PS,Lin CC,Hu MK,Huang SM,Wang YM,Liang CY,Gong Z,Ho YJ

更新日期：2017-09-23 00:00:00