Abstract:
:We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence revealed that neurocalcin is composed of 193 amino acids, has a molecular mass of 22,284 daltons, and contains three putative calcium-binding sites (EF-hand motifs). By Northern blot analysis, 3.8kbp mRNA was detected in brain. The deduced amino acid sequence had a strong homology to visinin (46.5%) and recoverin (51.6%) in retina, suggesting that neurocalcin may play a visinin- or recoverin-like role in brain.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Okazaki K,Watanabe M,Ando Y,Hagiwara M,Terasawa M,Hidaka Hdoi
10.1016/s0006-291x(05)80968-4subject
Has Abstractpub_date
1992-05-29 00:00:00pages
147-53issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(05)80968-4journal_volume
185pub_type
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