Abstract:
:A novel ribosome-inactivating protein designated beta-kirilowin was isolated from the seeds of Trichosanthes kirilowii. The proteins exhibited strong abortifacient activity in pregnant mice and produced a single band after SDS-polyacrylamide gel electrophoresis with an apparent mol. wt. of 27,500 daltons. It was active in inhibiting the cell-free translation system with an ID50 of about 1.8 ng/ml. No cross-reactivity with trichosanthin could be detected by immunodiffusion. Sequence comparison of the first 10 residues of beta-kirilowin with ribosome-inactivating proteins isolated from T. kirilowii such as trichosanthin, trichokirin and karasurin indicated 70%, 60% and 70% identity, respectively.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Dong TX,Ng TB,Yeung HW,Wong RNdoi
10.1006/bbrc.1994.1241subject
Has Abstractpub_date
1994-02-28 00:00:00pages
387-93issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)71241-1journal_volume
199pub_type
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