当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Human macrophages produce dimeric forms of IL-18 which can be detected with monoclonal antibodies specific for inactive IL-18.

Human macrophages produce dimeric forms of IL-18 which can be detected with monoclonal antibodies specific for inactive IL-18.

Abstract：

:We established two monoclonal antibodies (mAbs) which specifically recognize human 'functionally inactive' recombinant IL-18, and IL-18 protein polymorphism was examined using human monocytes and macrophages (M phi). In 6 day GM-CSF-treated M phi, an 'inactive' IL-18-recognizing mAb 21 detected the IL-18 proform (24 kDa) and a 48-kDa protein, which were gradually increased concomitant with maturation stage. Majority of the 24- and 48-kDa forms were barely detectable with other mAbs recognizing 'active' IL-18. No reagents including Toll stimulators up-regulated these IL-18 populations in M phi. The 21-recognizable IL-18 species were separated using an anion-exchanger column and their IFN gamma-inducing activity was assessed with human lymphocytes plus IL-12. Virtually no as yet known activity was detected with these IL-18 species. After processed with M phi proteases, an 18-kDa form was generated to express the IFN gamma-inducing activity, although the activity was far weaker than that of control 'active' IL-18. These observations suggested that large amounts of various IL-18 species are produced with monocyte-M phi differentiation and most of these IL-18 species are functionally 'inactive' in terms of the reported IL-18 function even after proteolytic 18-kDa conversion.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kikkawa S,Matsumoto M,Shida K,Fukumori Y,Toyoshima K,Seya T

doi

10.1006/bbrc.2001.4378

subject

Has Abstract

pub_date

2001-02-23 00:00:00

pages

461-7

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(01)94378-5

journal_volume

281

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Cytotoxicity of 6-biopterin to human melanocytes.

    abstract：:(6R)5,6,7,8 tetrahydrobiopterin (6-BH4) is an important cofactor in the regulation of melanogenesis in melanocytes, where it controls: (a) the supply of L-tyrosine from L-phenylalanine via phenylalanine hydroxylase, and (b) regulates directly dopaquinone formation from L-tyrosine via tyrosinase. 6-BH4 undergoes redox-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2423

    authors： Schallreuter KU,Büttner G,Pittelkow MR,Wood JM,Swanson NN,Körner C

    更新日期：1994-10-14 00:00:00

  • Thyroid hormone binding protein contains glycosylation site binding protein activity.

    abstract：:Several lines of evidence provided by other workers indicate that within the same species thyroid hormone binding protein, the beta-subunit of prolyl hydroxylase, and protein disulfide isomerase are the same protein. We sought to determine if glycosylation site binding protein, a lumenal protein of the endoplasmic ret...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90538-x

    authors： Kimura H,Noiva R,Mizunaga T,Yamauchi K,Horiuchi R,Cheng SY,Lennarz WJ

    更新日期：1990-08-16 00:00:00

  • Cloning, sequencing, and expression of an Escherichia coli acid phosphatase/phytase gene (appA2) isolated from pig colon.

    abstract：:Bacterial strains were isolated from the pig colon to screen for phytase and acid phosphatase activities. Among 93 colonies, Colony 88 had the highest activities for both enzymes and was identified as an Escherichia coli strain. Using primers derived from the E. coli pH 2.5 acid phosphatase appA sequence (Dassa et al....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0361

    authors： Rodriguez E,Han Y,Lei XG

    更新日期：1999-04-02 00:00:00

  • Ghrelin increases anxiety-like behavior and memory retention in rats.

    abstract：:Ghrelin is a peptide found in the hypothalamus and stomach that stimulates food intake and whose circulating concentrations are affected by nutritional state. Very little is known about other central behavioral effects of ghrelin, and thus, we investigated the effects of ghrelin on anxiety and memory retention. The pe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02740-7

    authors： Carlini VP,Monzón ME,Varas MM,Cragnolini AB,Schiöth HB,Scimonelli TN,de Barioglio SR

    更新日期：2002-12-20 00:00:00

  • Physical and functional interactions between protein tyrosine phosphatase alpha, PI 3-kinase, and PKCdelta.

    abstract：:The somatostatin analogue, TT-232 inhibits cell proliferation and induces apoptosis in a variety of tumor cells both in vivo and in vitro. While the early transient activation of Erk/MAPK was found to be important for the induction of cell cycle arrest, the signaling pathway leading to the activation of Erk/MAPK had n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5811

    authors： Steták A,Csermely P,Ullrich A,Kéri G

    更新日期：2001-11-02 00:00:00

  • Tissue- and stressor-specific differential expression of two hsc70 genes in carp.

    abstract：:Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitativ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01206-3

    authors： Ali KS,Dorgai L,Abrahám M,Hermesz E

    更新日期：2003-08-01 00:00:00

  • Silica-induced generation of reactive oxygen species in Rat2 fibroblast: role in activation of mitogen-activated protein kinase.

    abstract：:Exposure to silica has been associated with progressive pulmonary inflammation and fibrosis. While the fibroblasts play an important role in the pathogenesis of silicosis, the direct interaction between silica and fibroblasts is poorly understood. We observed that silica particles stimulated intracellular ROS generati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1274

    authors： Cho YJ,Seo MS,Kim JK,Lim Y,Chae G,Ha KS,Lee KH

    更新日期：1999-09-07 00:00:00

  • MIDA1, an Id-associating protein, has two distinct DNA binding activities that are converted by the association with Id1: a novel function of Id protein.

    abstract：:Id proteins not only regulate cell differentiation negatively, but they also promote growth, immortalization, and apoptosis. To know the mechanism of how Id regulates cell fate, we previously isolated an Id-associating protein, MIDA1, which positively regulates cell growth (1). Its predicted amino acid sequence consis...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1779

    authors： Inoue T,Shoji W,Obinata M

    更新日期：1999-12-09 00:00:00

  • Multi-frequency EPR evidence for a binuclear CuA center in cytochrome c oxidase: studies with a 63Cu- and 65Cu-enriched, soluble domain of the cytochrome ba3 subunit II from Thermus thermophilus.

    abstract：:We have recorded multi-frequency EPR spectra of 63Cu- and 65Cu-labeled, water-soluble CuA-protein from the cytochrome ba3 of T. thermophilus. The spectrum taken at the highest frequency (34.03 GHz) shows no hyperfine structure and is nominally axial with apparent gz approximately 2.18 and gxy approximately 2.00. The s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1938

    authors： Fee JA,Sanders D,Slutter CE,Doan PE,Aasa R,Karpefors M,Vänngård T

    更新日期：1995-07-06 00:00:00

  • Polyethylenimine-mediated suicide gene transfer induces a therapeutic effect for hepatocellular carcinoma in vivo by using an Epstein-Barr virus-based plasmid vector.

    abstract：:The present study aimed to establish a novel efficient nonviral strategy for suicide gene transfer in hepatocellular carcinoma (HCC) in vivo. We employed branched polyethylenimine (PEI) and combined it with Epstein-Barr virus (EBV)-based plasmid vectors. The HCC cells transfected with an EBV-based plasmid carrying the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6383

    authors： Iwai M,Harada Y,Tanaka S,Muramatsu A,Mori T,Kashima K,Imanishi J,Mazda O

    更新日期：2002-02-15 00:00:00

  • Overexpression of stearoyl-CoA desaturase-1 results in an increase of conjugated linoleic acid (CLA) and n-7 fatty acids in 293 cells.

    abstract：:The effect of human SCD1 heterologous expression on cellular fatty acid synthesis was investigated in the current study. The SCD1 gene expression cassette and PGK-neomycin-selectable marker cassette were co-introduced into HEK 293 cells by electroporation, and subsequently, SCD1 expression was evaluated by fatty acid ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.06.102

    authors： Wu Z,Li D,Gou K

    更新日期：2010-07-30 00:00:00

  • Role of CXC chemokines in the enhancement of LPS-induced neutrophil accumulation in the lung of mice by dexamethasone.

    abstract：:Lipopolysaccharide (LPS)-induced multiple organ injury was mediated in part by a transcription factor, nuclear factor-kappaB (NF-kappaB). Mice were pretreated with dexamethasone (DEX), an inhibitor of NF-kappaB activation, to elucidate its effects on LPS-induced early responses in vivo. Early responses measured 1 h af...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00573-9

    authors： Aoki K,Ishida Y,Kikuta N,Kawai H,Kuroiwa M,Sato H

    更新日期：2002-06-28 00:00:00

  • High-level expression in Escherichia coli and purification of recombinant plant profilins: comparison of IgE-binding capacity and allergenic activity.

    abstract：:Because of their structural similarity and ubiquitous distribution as actin binding proteins, plant profilins represent important cross-reactive allergens for almost 20% of patients suffering from Type I allergy to pollen and other plant products. The cDNAs coding for three birch profilin variants (Tyr44, Glu47, and A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1309

    authors： Vrtala S,Wiedemann P,Mittermann I,Eichler HG,Sperr WR,Valent P,Kraft D,Valenta R

    更新日期：1996-09-04 00:00:00

  • Endothelin-1 peptides and IL-5 synergistically increase the expression of IL-13 in eosinophils.

    abstract：:The 21-amino acid-length endothelin-1 (ET-1)(1-21) and its novel derivative, 31 amino acid-length ET-1(1-31), have proinflammatory properties and induce significant eosinophil migration mediated by an increase in the local levels of eotaxin and IL-5. We have analyzed by reverse transcription polymerase chain reaction ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.168

    authors： Cui P,Sharmin S,Okumura Y,Yamada H,Yano M,Mizuno D,Kido H

    更新日期：2004-03-19 00:00:00

  • Crystal structure and biochemical characterization of malate dehydrogenase from Metallosphaera sedula.

    abstract：:Metallosphaera sedula is a thermoacidophilic autotrophic archaeon and known to utilize the 3-hydroxypropionate/4-hydroxybutyrate cycle (3-HP/4-HB cycle) as a carbon fixation pathway. The 3-HP/4-HB cycle in M. sedula is associated with central metabolism, and malate dehydrogenase (MDH) is an enzyme involved in the cent...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.018

    authors： Lee D,Hong J,Kim KJ

    更新日期：2019-02-12 00:00:00

  • Identification of brain-specific angiogenesis inhibitor 2 as an interaction partner of glutaminase interacting protein.

    abstract：:The vast majority of physiological processes in living cells are mediated by protein-protein interactions often specified by particular protein sequence motifs. PDZ domains, composed of 80-100 amino acid residues, are an important class of interaction motif. Among the PDZ-containing proteins, glutaminase interacting p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.029

    authors： Zencir S,Ovee M,Dobson MJ,Banerjee M,Topcu Z,Mohanty S

    更新日期：2011-08-12 00:00:00

  • The role of active site residues in the oxidant specificity of the Orp1 thiol peroxidase.

    abstract：:In this study we investigated the role of active site residues in the peroxidase activity of Orp1 (GPx3) using three different peroxide substrates. Using a structural homology model of the reduced form of Orp1, we identified Asn126 and Phe127 as evolutionarily conserved residues that line the back of the Orp1 active s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.10.109

    authors： Takanishi CL,Ma LH,Wood MJ

    更新日期：2010-12-03 00:00:00

  • Wheat germ agglutinin-induced intracellular calcium mobilization in human platelets: suppression by staurosporine and resistance to cyclic AMP inhibition.

    abstract：:The lectin wheat germ agglutinin (WGA) elicited a prompt and sharp increase in intracellular Ca2+ concentration in human platelets. The WGA-induced Ca2+ mobilization was markedly inhibited by a protein kinase inhibitor staurosporine, whereas Ca2+ mobilization by receptor-mediated agonists, including thrombin, platelet...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1239

    authors： Yatomi Y,Ozaki Y,Koike Y,Satoh K,Kume S

    更新日期：1993-03-15 00:00:00

  • Involvement of P2X4 receptor in P2X7 receptor-dependent cell death of mouse macrophages.

    abstract：:Interaction of P2X7 receptor with P2X4 receptor has recently been suggested, but it remains unclear whether P2X4 receptor is involved in P2X7 receptor-mediated events, such as cell death of macrophages induced by high concentrations of extracellular ATP. Here, we present evidence that P2X4 receptor does play a role in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.156

    authors： Kawano A,Tsukimoto M,Noguchi T,Hotta N,Harada H,Takenouchi T,Kitani H,Kojima S

    更新日期：2012-03-09 00:00:00

  • Oxidative DNA damage by a metabolite of carcinogenic and reproductive toxic nitrobenzene in the presence of NADH and Cu(II).

    abstract：:The mechanism of DNA damage induced by metabolites of nitrobenzene was investigated in relation to the carcinogenicity and reproductive toxicity of nitrobenzene. Nitrosobenzene, a nitrobenzene metabolite, induced NADH plus Cu(II)-mediated DNA cleavage frequently at thymine and cytosine residues. Catalase and bathocupr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0525

    authors： Ohkuma Y,Kawanishi S

    更新日期：1999-04-13 00:00:00

  • A specific association of ERK3 with B-Raf in rat hippocampus.

    abstract：:The possible formation of a stable complex between extracellular signal-regulated kinases (ERKs) and Raf protein kinases was investigated in an attempt to understand the molecular mechanism by which Raf protein kinases were differentially regulated in response to the various stimuli, leading to the activation of ERKs ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1846

    authors： Kim SH,Yang YC

    更新日期：1996-12-13 00:00:00

  • Androgen receptor regulates cardiac fibrosis in mice with experimental autoimmune myocarditis by increasing microRNA-125b expression.

    abstract：:Cardiac fibrosis is an important cardiac remodeling event in the development of inflammation dilated cardiomyopathy （iDCM）. We have previously observed that degradation enhancer of androgen receptor (ASC-J9®) could improve cardiac inflammation and fibrosis. Using Primary CFs, we demonstrated that ASC-J9® attenuates th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.092

    authors： Wang Y,Ma W,Lu S,Yan L,Hu F,Wang Z,Cheng B

    更新日期：2018-11-17 00:00:00

  • BRG1 is indispensable for IFN-γ-induced TRIM22 expression, which is dependent on the recruitment of IRF-1.

    abstract：:The modification of chromatin structure is increasingly recognized to be an important facet of transcriptional regulation. Here, we report that Brahma-related gene 1 (BRG1), a chromatin remodeling enzyme, plays a crucial role in IFN-γ-induced TRIM22 expression. Our results showed that IFN-γ failed to induce TRIM22 exp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.022

    authors： Wang Y,Gao B,Xu W,Xiong S

    更新日期：2011-07-08 00:00:00

  • Fluorescein fluorescence hyperpolarization as an early kinetic measure of the apoptotic process.

    abstract：:The ability to identify apoptotic cells within a complex population is crucial in the research and diagnosis of normal physiology and disease states. The Cellscan mark S (CS-S) cytometer was used in this study to detect intracellular fluorescence intensity and polarization (FI and FP) in several well-established model...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1934

    authors： Zurgil N,Schiffer Z,Shafran Y,Kaufman M,Deutsch M

    更新日期：2000-02-05 00:00:00

  • Identification of genes responsive to low-intensity pulsed ultrasound stimulations.

    abstract：:This study was designed to compare the temporal changes of gene expression profile in osteoblastic cell lines (SaOS-2) treated with low-intensity pulsed ultrasound stimulation (LIPUS) using complementary DNA (cDNA) microarrays. SaOS-2 cells were treated with LIPUS for 20min. Thereafter, cells were harvested and RNA wa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.074

    authors： Lu H,Qin L,Lee K,Cheung W,Chan K,Leung K

    更新日期：2009-01-16 00:00:00

  • Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN.

    abstract：:The mouse disabled 2 interacting protein 2 (mDaIP2) had been obtained through yeast two hybrid system. It consists of 506 amino acids and its calculated molecular weight is 57.7 kDa. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain interacts with the proline rich domain of mDab2 whi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.11.079

    authors： Choi YJ,Cho SY,Kim HW,Kim JA,Bae SH,Park SS

    更新日期：2005-01-21 00:00:00

  • DJ-1 overexpression confers the multidrug resistance phenotype to SGC7901 cells by upregulating P-gp and Bcl-2.

    abstract：:Gastric cancer (GC) is one of the most malignant tumors with high incidence and mortality worldwide, and the multidrug resistance (MDR) often results in chemotherapy failure in GC. DJ-1 has been well indicated to be associated with drug resistance in multiple cancers. However, the role of DJ-1 in the MDR of gastric ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.131

    authors： Liu HY,Duan GL,Xu RY,Li XR,Xiao L,Zhao L,Ma ZX,Xu XW,Qiu LJ,Zhu ZM,Chen HP

    更新日期：2019-10-29 00:00:00

  • The inhibition of sugar-induced structural alterations in collagen by aspirin and other compounds.

    abstract：:With age human collagen demonstrates, amongst other changes, reductions in solubility, elasticity and permeability. Many of these changes have been attributed to non-enzymic glycosylation (glycation)-a spontaneous addition of sugar molecules to any protein with free amino groups. The resulting formation and accumulati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1282

    authors： Malik NS,Meek KM

    更新日期：1994-03-15 00:00:00

  • Interaction of soluble CD4 with the chemokine receptor CCR5.

    abstract：:The chemokine receptor CCR5 is constitutively associated with the T cell co-receptor CD4 in plasma cell membranes. The CD4-CCR5 complex exhibits distinct binding properties for macrophage inflammatory protein 1beta (MIP-1beta) and enhanced G-protein signaling as compared with those of CCR5 alone. Here we report that r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01315-9

    authors： Wang X,Staudinger R

    更新日期：2003-08-08 00:00:00

  • Establishment of the ELISA for murine soluble gp130, a signal transducer for the IL-6 family cytokine, and its detection in the ascitic fluids of tumor-bearing mice.

    abstract：:Interleukin-6 (IL-6) signal is transduced through a membrane glycoprotein gp130, which associates with the IL-6 receptor alpha chain (IL-6R alpha) in the presence of IL-6. We prepared monoclonal antibodies (mAbs) specific to murine gp130 by immunizing rats and hamsters with a chimeric molecule consisting of the extrac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1975

    authors： Matsuda T,Hirano T

    更新日期：1994-07-15 00:00:00