Development of an affinity chromatography resin for the purification of carcinogen binding proteins from mouse liver.

Abstract:

:Pyrene, a structural analog of benzo[a]pyrene, is an effective competing ligand for high affinity carcinogen binding proteins in mouse liver. A pyrene-derivatized Sepharose gel was prepared for affinity chromatography purification of these proteins, and adsorbs all detectable [3H]B[a]P-binding activity from hepatic cytosol with the adsorption of less than 1% of total protein. Specific carcinogen binding activity is recovered from pyrene-derivatized Sepharose columns with the enrichment of a 33 kDa polypeptide. This chromatography resin represents a major step in the isolation of these unusual receptor-like binding proteins for aromatic hydrocarbon carcinogens.

authors

Collins S,Altman JD,Marletta MA

doi

10.1016/0006-291x(85)91416-0

subject

Has Abstract

pub_date

1985-05-31 00:00:00

pages

155-62

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(85)91416-0

journal_volume

129

pub_type

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