IL-17A modulates osteoclast precursors' apoptosis through autophagy-TRAF3 signaling during osteoclastogenesis.


:Osteoclasts play an important role in bone remodeling. The inflammatory cytokine IL-17A could modulate the RANKL-induced osteoclastogenesis by regulating the autophagic activity. It is well accepted that protective autophagy has an anti-apoptotic effect. It is necessary to elucidate whether IL-17A can influence the apoptosis of osteoclast precursors (OCPs) through autophagy responses during osteoclastogenesis. The results showed that apoptosis of RAW264.7-derived OCPs was promoted by high levels of IL-17A, but the opposite anti-apoptotic function was shown by low levels of IL-17A. Furthermore, the enhanced apoptosis by high levels of IL-17A was reversed by overexpression of autophagy protein Beclin1; conversely, the inhibited apoptosis by low levels of IL-17A was restored by knockdown of Beclin1. It was also found that Beclin1 suppression with Beclin1 inhibitor (spautin1) could block the reduced apoptosis by low levels of IL-17A, which was recovered by TRAF3 knockdown. Moreover, the enhanced apoptosis by high levels of IL-17A decreased following the downregulation of TRAF3. Importantly, overexpression of caspase3 further attenuated osteoclastogenesis treated by high levels of IL-17A, without significantly affecting osteoclastogenesis stimulated by low levels of IL-17A. In conclusion, IL-17A modulates apoptosis of OCPs through Beclin1-autophagy-TRAF3 signaling pathway, thereby influencing osteoclastogenesis. Therefore, our study sheds lights on the improvement of clinical strategies of dental implantation or orthodontic treatment by revealing the novel targets in the bone remodeling.


Xue Y,Liang Z,Fu X,Wang T,Xie Q,Ke D




Has Abstract


2019-01-22 00:00:00














  • Antiviral effects of two Ganoderma lucidum triterpenoids against enterovirus 71 infection.

    abstract::Enterovirus 71 (EV71) is a major causative agent for hand, foot and mouth disease (HFMD), and fatal neurological and systemic complications in children. However, there is currently no clinical approved antiviral drug available for the prevention and treatment of the viral infection. Here, we evaluated the antiviral ac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang W,Tao J,Yang X,Yang Z,Zhang L,Liu H,Wu K,Wu J

    更新日期:2014-07-04 00:00:00

  • A DNA-binding protein from E. coli isolation, characterization and its relationship with proteins H1 and B1.

    abstract::A protein of Mr 16,000 (protein 16 K) which tightly binds to DNA has been isolated from E. coli. The amino acid composition and amino-terminal amino acid sequence of this protein have been determined. On the basis of its physico-chemical characteristics the protein 16 K was shown to be identical to the protein H1 [Cuk...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Laine B,Sautiere P,Spassky A,Rimsky S

    更新日期:1984-03-30 00:00:00

  • UDP-glucuronosyltransferase 1A7 polymorphisms are associated with liver cirrhosis.

    abstract::Variations in the UDP-glucuronosyltransferase (UGT) 1A7 gene have been found to be related to the development of hepatocellular carcinoma (HCC). Since the pathogenesis of liver cirrhosis is not dissimilar to that of HCC, we hypothesized that UGT1A7 genetic polymorphisms may be associated with liver cirrhosis. PCR-rest...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tang KS,Lee CM,Teng HC,Huang MJ,Huang CS

    更新日期:2008-02-15 00:00:00

  • Inositol tetrakisphosphates as second messengers induce Ca(++)-dependent chloride currents in Xenopus laevis oocytes.

    abstract::Microinjection of inositol 1,3,4,5-tetrakisphosphate or inositol 1,4,5-trisphosphate induced distinct chloride membrane currents in defolliculated Xenopus laevis oocytes. To decide whether these Cl(-)-currents were due to the injected compounds or their metabolic products, [3H]Ins(1,3,4,5)P4 or [3H]Ins(1,4,5)P3 were i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Guse AH,Gercken G,Boysen H,Schwarz JR,Meyerhof W

    更新日期:1991-08-30 00:00:00

  • Structure and chromosomal locus of the mouse gene encoding a cerebellar Purkinje cell-specific helix-loop-helix factor Hes-3.

    abstract::HES-3 is a cerebellar Purkinje cell-specific helix-loop-helix factor structurally related to the products of the Drosophila hairy and Enhancer of split genes. Here, we report the nucleotide sequence and chromosomal locus of the mouse Hes-3 gene. This gene consists of four exons and the exon-intron boundaries are well ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakagami T,Sakurada K,Sakai Y,Watanabe T,Nakanishi S,Kageyama R

    更新日期:1994-08-30 00:00:00

  • A chemically defined culture medium containing Rho kinase inhibitor Y-27632 for the fabrication of stratified squamous epithelial cell grafts.

    abstract::With the development of a culture method for stratified squamous epithelial cells, tissue-engineered epithelial cell sheets have been successfully applied as clinical cell grafts. However, the implementation of these cell sheets without the use of any animal-derived materials is highly desirable. In this study, Rho-as...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Aslanova A,Takagi R,Yamato M,Okano T,Yamamoto M

    更新日期:2015-05-01 00:00:00

  • A method for specific cloning and sequencing of human hprt cDNA for mutation analysis.

    abstract::A method has been developed for the specific amplification and cloning of human hprt cDNA which can be used for mutant sequence analysis. Messenger RNA is isolated from TK6 lymphoblasts and is used to produce a first strand cDNA with reverse transcriptase primed with oligo dT. Second strand synthesis and subsequent am...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Simpson D,Crosby RM,Skopek TR

    更新日期:1988-02-29 00:00:00

  • Complete coding sequence of the Alkhurma virus, a tick-borne flavivirus causing severe hemorrhagic fever in humans in Saudi Arabia.

    abstract::To date, tick-borne flaviviruses responsible for hemorrhagic fever in humans have been isolated in Siberia (Omsk hemorrhagic fever virus), India (Kyasanur Forest disease virus, KFDV), and in Saudi Arabia (Alkhurma virus, ALKV). Prior to this study, only partial coding sequences of these severe pathogens had been deter...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Charrel RN,Zaki AM,Attoui H,Fakeeh M,Billoir F,Yousef AI,de Chesse R,De Micco P,Gould EA,de Lamballerie X

    更新日期:2001-09-21 00:00:00

  • Superior serum half life of albumin tagged TNF ligands.

    abstract::Due to their immune stimulating and apoptosis inducing properties, ligands of the TNF family attract increasing interest as therapeutic proteins. A general limitation of in vivo applications of recombinant soluble TNF ligands is their notoriously rapid clearance from circulation. To improve the serum half life of the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Müller N,Schneider B,Pfizenmaier K,Wajant H

    更新日期:2010-06-11 00:00:00

  • Transient inositol (1,4,5) trisphosphate accumulation under vasopressin stimulation in WRK1 cells: correlation with intracellular calcium mobilization.

    abstract::In the rat mammary tumoral cell line (WRK1 cells), vasopressin was previously described to stimulate a phospholipase C. In this study, we have analysed the effect of vasopressin both on intracellular calcium mobilization and on the accumulation of inositol phosphates. Maximal concentration of vasopressin simultaneousl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mouillac B,Balestre MN,Guillon G

    更新日期:1989-03-31 00:00:00

  • Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists.

    abstract::The role of PPARs in the regulation of human adipose tissue secretome has received little attention despite its potential importance in the therapeutic actions of PPAR agonists. Here, we have investigated the effect of selective PPARgamma, PPARalpha, and PPARbeta/delta agonists on the production of adipokines by human...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Klimcakova E,Moro C,Mazzucotelli A,Lolmède K,Viguerie N,Galitzky J,Stich V,Langin D

    更新日期:2007-07-06 00:00:00

  • Organization of human ACAT-2 gene and its cell-type-specific promoter activity.

    abstract::Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Song BL,Qi W,Yang XY,Chang CC,Zhu JQ,Chang TY,Li BL

    更新日期:2001-03-30 00:00:00

  • Identification of a region critical for proteolysis of the human growth hormone receptor.

    abstract::Release of soluble growth hormone binding protein (GHBP) corresponding to the extracellular domain of the GH receptor (GHR) occurs via distinct mechanisms depending on species. In human, proteolysis of full length GHR results in liberation of GHBP into the extracellular medium. A putative protease responsive for GHR c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Conte F,Salles JP,Raynal P,Fernandez L,Molinas C,Tauber M,Bieth E

    更新日期:2002-01-18 00:00:00

  • Phospholipase D is associated in a phorbol ester-dependent manner with protein kinase C-alpha and with a 220-kDa protein which is phosphorylated on serine and threonine.

    abstract::Many studies have shown that phospholipase D (PLD) is activated by protein kinase C (PKC) in vivo and in vitro. In this study, a PLD isoform (rPLD1) was shown to bind to PKC-alpha in Rat1 fibroblasts treated with phorbol ester. The PKC-alpha binding domain of rPLD1 was localized to its N-terminus. The phospholipase wa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Min DS,Exton JH

    更新日期:1998-07-30 00:00:00

  • Bone morphogenetic protein.

    abstract::Bone morphogenetic proteins (BMPs) are multi-functional growth factors belonging to the transforming growth factor-beta superfamily. It has been demonstrated that BMPs had been involved in the regulation of cell proliferation, survival, differentiation and apoptosis. However, their hallmark ability is that play a pivo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Xiao YT,Xiang LX,Shao JZ

    更新日期:2007-10-26 00:00:00

  • Swings in blood glucose levels accelerate atherogenesis in apolipoprotein E-deficient mice.

    abstract::The aim of this study was to investigate the effect of fluctuations in blood glucose levels on atherogenesis. Apolipoprotein (apo) E-deficient mice fed maltose twice daily were used as a model of repetitive postprandial glucose spikes. We investigated the number of macrophages adherent to the endothelium and the area ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mita T,Otsuka A,Azuma K,Uchida T,Ogihara T,Fujitani Y,Hirose T,Mitsumata M,Kawamori R,Watada H

    更新日期:2007-07-06 00:00:00

  • Archaeoglobus fulgidus RNase HII in DNA replication: enzymological functions and activity regulation via metal cofactors.

    abstract::RNA primer removal during DNA replication is dependent on ribonucleotide- and structure-specific RNase H and FEN-1 nuclease activities. A specific RNase H involved in this reaction has long been sought. RNase HII is the only open reading frame in Archaeoglobus fulgidus genome, while multiple RNases H exist in eukaryot...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chai Q,Qiu J,Chapados BR,Shen B

    更新日期:2001-09-07 00:00:00

  • Monoallelic expression of MAOA in skin fibroblasts.

    abstract::X chromosome inactivation in mammalian females occurs early in embryonic development and renders most genes on the inactive X chromosome transcriptionally silenced. As a consequence, females will display an X chromosomal parent-of-origin mosaiscism with regard to which parental allele that is expressed. Some genes, ho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nordquist N,Oreland L

    更新日期:2006-09-22 00:00:00

  • The mechanism of action of DuP 721, a new antibacterial agent: effects on macromolecular synthesis.

    abstract::Pulse labeling studies with Bacillus subtilis showed that DuP 721 inhibited protein synthesis. The IC50 of DuP 721 for protein synthesis was 0.25 micrograms/ml but it was greater than 32 micrograms/ml for RNA and DNA synthesis. In cell-free systems, DuP 721 concentrations up to 100 microM did not inhibit peptide chain...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Eustice DC,Feldman PA,Slee AM

    更新日期:1988-02-15 00:00:00

  • alpha-Difluoromethylornithine does not bind to ornithine decarboxylase-antizyme complex.

    abstract::alpha-Difluoromethylornithine (DFMO) has been widely used for determining the amounts of ornithine decarboxylase (ODC) in cells. However, we have now found that DFMO does not react with ODC-antizyme complex, indicating that the amounts of radioactive DFMO bound to the protein may not reflect the amounts of ODC in crud...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kitani T,Fujisawa H

    更新日期:1986-06-30 00:00:00

  • Nuclear localization of tumor necrosis factor-alpha in human osteoblast-like cells.

    abstract::We have studied the internalization and subsequent nuclear localization of [125I]-rTNF-alpha in a human osteoblast-like cell line, HOS TE85. TNF-alpha is rapidly internalized and optimum cellular levels are achieved in approximately 20 minutes at 37 degrees C. A portion of the internalized cytokine is translocated to ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Panagakos FS,Kumar S

    更新日期:1994-06-30 00:00:00

  • The anti-obesity effect of quercetin is mediated by the AMPK and MAPK signaling pathways.

    abstract::Quercetin is the most abundant flavonoid and is assumed to have protective roles against the pathogenesis of multiple diseases associated with oxidative stress. In the present study, we investigated the molecular mechanisms by which quercetin affects adipogenesis and apoptosis in 3T3-L1 cells. The exposure of 3T3-L1 p...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ahn J,Lee H,Kim S,Park J,Ha T

    更新日期:2008-09-05 00:00:00

  • Downregualtion of dynamin-related protein 1 attenuates glutamate-induced excitotoxicity via regulating mitochondrial function in a calcium dependent manner in HT22 cells.

    abstract::Glutamate-mediated excitotoxicity is involved in many acute and chronic brain diseases. Dynamin related protein 1 (Drp-1), one of the GTPase family of proteins that regulate mitochondrial fission and fusion balance, is associated with apoptotic cell death in cancer and neurodegenerative diseases. Here we investigated ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang C,Yuan XR,Li HY,Zhao ZJ,Liao YW,Wang XY,Su J,Sang SS,Liu Q

    更新日期:2014-01-03 00:00:00

  • WISP-2 as a novel estrogen-responsive gene in human breast cancer cells.

    abstract::In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Inadera H,Hashimoto S,Dong HY,Suzuki T,Nagai S,Yamashita T,Toyoda N,Matsushima K

    更新日期:2000-08-18 00:00:00

  • The role of adenine nucleotide translocation in the energization of the inner membrane of mitochondria isolated from rho + and rho degree strains of Saccharomyces cerevisiae.

    abstract::The energization of rho + and rho degrees isolated mitochondria was measured using a tetraphenylphosphonium selective electrode. In both strains translocase mediated ATP/ADP exchange energization was observed. This energization was more sensitive to uncoupler than that induced by respiration in rho + mitochondria. Thi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dupont CH,Mazat JP,Guerin B

    更新日期:1985-11-15 00:00:00

  • Hypertonic induction of COX2 expression requires TonEBP/NFAT5 in renal epithelial cells.

    abstract::TonEBP/NFAT5 transcription factor is a master regulator of genes involved in osmoprotection. Cyclooxygenase 2 (COX2) has been reported to be a cytoprotective molecule in the inner renal medulla, where cells are physiologically exposed to the highest osmolality of the body. Our aim was to study whether COX2 expression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Favale NO,Casali CI,Lepera LG,Pescio LG,Fernández-Tome MC

    更新日期:2009-04-10 00:00:00

  • Potentiation of monovalent cation effects on ligand binding to cardiac muscarinic receptors in N-ethylmaleimide treated membranes.

    abstract::Guanine nucleotides and monovalent cations decrease the affinity of cardiac muscarinic receptors for agonists and are required for muscarinic receptor mediated inhibition of adenylate cyclase. N-ethylmaleimide abolished the effects of Gpp(NH)p on the ability of the agonist oxotremorine to inhibit the binding of the an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: McMahon KK,Hosey MM

    更新日期:1983-02-28 00:00:00

  • Involvement of CREB in the transcriptional regulation of the human GM3 synthase (hST3Gal V) gene during megakaryocytoid differentiation of human leukemia K562 cells.

    abstract::We studied the transcriptional regulation of human GM3 synthase (hST3Gal V) during megakaryocytic differentiation of K562 cells induced by PMA. Northern blot and reverse transcription polymerase chain reaction (RT-PCR) indicated that the induction of hST3Gal V by phorbol 12-myristate 13-acetate (PMA) is regulated at t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Choi HJ,Chung TW,Kang NY,Kim KS,Lee YC,Kim CH

    更新日期:2004-01-02 00:00:00

  • Epidermal growth factor receptor mediated proliferation depends on increased lipid droplet density regulated via a negative regulatory loop with FOXO3/Sirtuin6.

    abstract::The proliferation of colon cancer cells is mediated in part by epidermal growth factor receptor (EGFR) signaling and requires sustained levels of cellular energy to meet its high metabolic needs. Intracellular lipid droplets (LDs) are a source of energy used for various cellular functions and they are elevated in dens...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Penrose H,Heller S,Cable C,Makboul R,Chadalawada G,Chen Y,Crawford SE,Savkovic SD

    更新日期:2016-01-15 00:00:00

  • Endothelin induced collagen remodeling in experimental pulmonary hypertension.

    abstract::To investigate pathophysiological roles of endothelin-1 (ET-1) in collagen remodeling in pulmonary hypertension, we measured: (a) mRNA expression, concentration, localization of ET-1; (b) changes in types and content of collagen in the lung; (c) and confirmed direct effects of ET-1 on type V collagen metabolism in vas...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mansoor AM,Honda M,Saida K,Ishinaga Y,Kuramochi T,Maeda A,Takabatake T,Mitsui Y

    更新日期:1995-10-24 00:00:00