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Nested-polymerase chain reaction for the detection of Helicobacter pylori infection with novel primers designed by sequence analysis of urease A gene in clinically isolated bacterial strains.

Abstract：

:We have established a highly sensitive semi-nested PCR assay for the detection of H. pylori infection using gastric juice samples, which can be aspirated with disposable nasogastric tubes. The primers targeting H. pylori urease A gene were designed based on the sequence conservation analysis of sixteen H. pylori strains isolated from Japanese patients. The efficacy of the PCR assay, designated as the URA-PCR, was confirmed by in vitro and in vivo assessments. Its sensitivity was 97.5% in the gastric juice samples aspirated from forty patients with proven H. pylori infection, and was significantly higher than that obtained with previously described PCR assays.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kawamata O,Yoshida H,Hirota K,Yoshida A,Kawaguchi R,Shiratori Y,Omata M

doi

10.1006/bbrc.1996.0216

subject

Has Abstract

pub_date

1996-02-06 00:00:00

pages

266-72

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(96)90216-8

journal_volume

219

pub_type

杂志文章

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