Abstract:
:Genomic DNA analysis revealed that the coding region of the rat histamine H3 receptor comprises three exons interrupted by two introns of approximately 1 kb each. Several H3 receptor mRNA variants were identified by PCR and cDNA cloning and sequencing. Four variants generated by pseudo-intron retention/deletion at the level of the third intracellular loop were designated H3(445), H3(413), H3(410), and H3(397), according to the length of their deduced amino acid sequence and display differential tissue expression. When expressed in CHO-K1 or Cos-1 cells, the H3(445), H3(413), and H3(397) were found to generate specific 125I iodoproxyfan binding of similar pharmacological profile. In addition, we identified two short variants, termed H3(nf1) and H3(nf2), which correspond to frame shift and stop codon interposition, respectively, and are presumably nonfunctional, among which H3(nf2) displays brain expression similar to that of the longer isoforms.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Morisset S,Sasse A,Gbahou F,Héron A,Ligneau X,Tardivel-Lacombe J,Schwartz JC,Arrang JMdoi
10.1006/bbrc.2000.4073subject
Has Abstractpub_date
2001-01-12 00:00:00pages
75-80issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(00)94073-7journal_volume
280pub_type
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