当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Cloning and expression of genes coding for carbohydrate degrading enzymes of Anaerocellum thermophilum in E.coli.

Cloning and expression of genes coding for carbohydrate degrading enzymes of Anaerocellum thermophilum in E.coli.

Abstract：

:A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screening for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clones expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. Clones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bolshakova EV,Ponomariev AA,Novikov AA,Svetlichnyi VA,Velikodvorskaya GA

doi

10.1006/bbrc.1994.2038

subject

Has Abstract

pub_date

1994-07-29 00:00:00

pages

1076-80

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X84720389

journal_volume

202

pub_type

杂志文章

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