RhoC is essential for TGF-beta1-induced invasive capacity of rat ascites hepatoma cells.

Abstract:

:Transforming growth factor-beta1 (TGF-beta1) is a multifunctional growth factor that plays a role in cell proliferation, differentiation, extracellular matrix production, apoptosis, and cell motility. We show here that TGF-beta1 increased the invasiveness of MM1 cells, which are a highly invasive clone of rat ascites hepatoma cells. Both mRNA and protein levels of RhoC but not RhoA in TGF-beta1-treated MM1 cells increased. In parallel with this increase in expression, RhoC activity was induced by TGF-beta1 treatment. When RhoC was overexpressed in MM1 cells, the invasive capacity increased. The RhoC-overexpressing cells formed more nodules than did mock cells when injected into rat peritoneum. Furthermore, when RhoC expression was reduced by transfection with shRNA/RhoC, the invasiveness of MM1 cells decreased with concomitant suppression of RhoC expression. Thus, the induced expression of RhoC by TGF-beta1 in MM1 cells plays a critical role in TGF-beta1-induced cell migration.

authors

Mukai M,Endo H,Iwasaki T,Tatsuta M,Togawa A,Nakamura H,Inoue M

doi

10.1016/j.bbrc.2006.05.068

subject

Has Abstract

pub_date

2006-07-21 00:00:00

pages

74-82

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)01126-0

journal_volume

346

pub_type

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