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Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes.

Abstract：

:Chondrocyte mechanotransduction in response to mechanical loading is essential for the health and homeostasis of articular cartilage. The actin cytoskeleton has been implicated in cell mechanics and mechanotransduction. This study tests the hypothesis that loading modulates actin dynamics and organisation with subsequent changes in gene expression for actin associated proteins. Chondrocytes were transfected with eGFP-actin, seeded in agarose and subjected to cyclic compression (10 cycles, 1 Hz, 0-15% strain) on the stage of a confocal microscope. Compression resulted in a subsequent reduction in cortical eGFP-actin intensity and a reduction in fluorescence recovery after photobleaching (FRAP), suggesting net cortical actin de-polymerisation, compared to unloaded controls. Cyclic compression for 10 min up-regulated gene expression for the actin depolymerising proteins, cofilin and destrin. Thus mechanical loading alters cortical actin dynamics, providing a potential mechanism through which chondrocytes can adapt their mechanical properties and mechanosensitivity to the local mechanical environment.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Campbell JJ,Blain EJ,Chowdhury TT,Knight MM

doi

10.1016/j.bbrc.2007.06.185

subject

Has Abstract

pub_date

2007-09-21 00:00:00

pages

329-34

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(07)01436-2

journal_volume

361

pub_type

杂志文章

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