Abstract:
:The anomeric proton of the reducing N-acetylglucosamine residue of beta-N,N'-diacetylchitobiose exhibited an unusual lineshape indicative of virtual coupling in the 250 MHz proton NMR spectrum. The two-dimensional J-resolved spectrum contained four lines associated with this proton, and this pattern can be used to distinguish virtual coupling from other effects. Anomeric protons of previously studied asparagine-linked glycopeptides which exhibited unusual lineshapes in the conventional NMR spectra did not exhibit the four line patterns indicative of virtual coupling in the two-dimensional J-resolved spectra. Although virtual coupling may partially account for the unusual lineshapes observed in the normal proton NMR spectra, the anomalous behavior observed in the two-dimensional J-resolved spectra of these glycopeptides cannot be explained solely by this phenomenon.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Bruch RC,Bruch MD,Noggle JH,White HB 3rddoi
10.1016/0006-291x(84)90265-1subject
Has Abstractpub_date
1984-09-17 00:00:00pages
555-61issue
2eissn
0006-291Xissn
1090-2104pii
0006-291X(84)90265-1journal_volume
123pub_type
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