Preparation of monomeric cytochrome C oxidase: its kinetics differ from those of the dimeric enzyme.

Abstract:

:Bovine cytochrome c oxidase in 0.1% dodecylmaltoside, 50 mM KCl and 10 mM Tris-HCl, pH 7.4 is monodisperse with an apparent Mr 360,000 (dimer) as estimated by filtration on Ultrogel AcA 34. In the absence of added KCl the apparent Mr is 160,000 (monomer). The dimeric enzyme has a high and a low affinity site for cytochrome c; the monomeric, only the high affinity site. The results are consistent with the existence of one active site per monomer, having high affinity for cytochrome c. Since in a dimer the two sites are in close proximity, the binding of the first molecule of cytochrome c to the first site hinders the binding of the second molecule to the second site. The kinetic data fit with a model of homotropic negative cooperativity. The effect of salts on the cytochrome c oxidase kinetics is also present in isolated bovine heart mitochondria.

authors

Nałecz KA,Bolli R,Azzi A

doi

10.1016/0006-291x(83)90855-0

subject

Has Abstract

pub_date

1983-07-29 00:00:00

pages

822-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)90855-0

journal_volume

114

pub_type

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