Abstract:
:Four analogues of parathyroid hormone-related protein (PTHrP), PTHrP(7-34)NH2, (10-34)NH2, (15-34)NH2 and (20-34)NH2, were synthesized and their antagonistic activity against PTHrP(1-34) was examined in vitro and in vivo. In vitro studies revealed that all four analogues antagonized PTHrP-stimulated cyclic AMP production in rat osteosarcoma cells (ROS 17/2.8), and that PTHrP(7-34)NH2 and PTHrP(10-34)NH2 had potent antagonistic activity. In vivo experiments in nude mice also revealed that PTHrP(7-34)NH2 completely inhibited hypercalcemia induced by PTHrP(1-34), indicating that these analogues antagonize the effects of PTHrP(1-34) in vitro and in vivo.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Nagasaki K,Yamaguchi K,Miyake Y,Hayashi C,Honda S,Urakami K,Miki K,Kimura S,Watanabe T,Abe Kdoi
10.1016/0006-291x(89)92826-xsubject
Has Abstract,Author List Incompletepub_date
1989-02-15 00:00:00pages
1036-42issue
3eissn
0006-291Xissn
1090-2104pii
0006-291X(89)92826-Xjournal_volume
158pub_type
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