Differential control of activin, inhibin and follistatin proteins in cultured rat granulosa cells.

abstract：:The ubiquitously expressed Calpains 1 and 2 belong to a family of calcium-dependent intracellular cysteine proteases. Both calpains are heterodimers consisting of a large subunit and a small regulatory subunit encoded by the gene Capns1. To investigate a role for the calpain small subunit in cells of the osteoblast li...

journal_title：Biochemical and biophysical research communications

authors： Kashiwagi A,Fein MJ,Shimada M

更新日期：2011-06-03 00:00:00

abstract：:The objective of this study is to determine whether Rab7 could delay intervertebral disc degeneration and study the mechanism. We chose old mice to established intervertebral disc degeneration model and study the effect of Rab7 on intervertebral disc by western blotting, quantitative real-time PCR, immunohistochemistr...

journal_title：Biochemical and biophysical research communications

authors： Chen X,Zhang P,Ma X

更新日期：2019-06-30 00:00:00

abstract：:Previous studies have demonstrated expression of Toll-like receptors (TLRs) in the surface epithelium of normal ovaries (OSE) and in epithelial ovarian tumors. Most notably, OSE-derived cancers express TLR4, which activates the nuclear factor-kappa B (NF-κB) signaling cascade as a mediator of inflammatory response. Cu...

journal_title：Biochemical and biophysical research communications

authors： Woods DC,White YA,Dau C,Johnson AL

更新日期：2011-06-17 00:00:00

abstract：:Lipocortin I-S100 (calcyclin) heterotetramer exhibited ATPase activity in the presence of dsDNA but not ssDNA. To demonstrate its helicase activity, an 80-mer polynucleotide complementary to the replication origin of M13mp18 was synthesized, and the oligonucleotide, (dC)(20), was ligated to either its 5'- or 3'- end f...

journal_title：Biochemical and biophysical research communications

authors： Hirata A,Hirata F

更新日期：2002-02-22 00:00:00

abstract：:The possible formation of a stable complex between extracellular signal-regulated kinases (ERKs) and Raf protein kinases was investigated in an attempt to understand the molecular mechanism by which Raf protein kinases were differentially regulated in response to the various stimuli, leading to the activation of ERKs ...

journal_title：Biochemical and biophysical research communications

authors： Kim SH,Yang YC

更新日期：1996-12-13 00:00:00

abstract：:Pyrrolidine dithiocarbamate (PDTC) is a synthetic antioxidant molecule, which has been recently proposed as an antitumoral agent on the basis of its capability of inducing apoptosis. We investigated the effect of PDTC on the proliferation and survival of the promyelocitic cell line HL-60. Concentration as low as 10 mi...

journal_title：Biochemical and biophysical research communications

authors： Della Ragione F,Cucciolla V,Borriello A,Della Pietra V,Manna C,Galletti P,Zappia V

更新日期：2000-02-24 00:00:00

abstract：:Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG bi...

journal_title：Biochemical and biophysical research communications

authors： Ojala K,Mottershead DG,Suokko A,Oker-Blom C

更新日期：2001-06-15 00:00:00

abstract：:It has recently been reported that expression of heme oxygenase-1 (HO-1) plays a protective role against many diseases. Furthermore, n-3 polyunsaturated fatty acids (PUFAs) were shown to induce HO-1 expression in several cells in vitro, and in a few cases also in vivo. However, very few reports have demonstrated that ...

journal_title：Biochemical and biophysical research communications

authors： Nakagawa F,Morino K,Ugi S,Ishikado A,Kondo K,Sato D,Konno S,Nemoto K,Kusunoki C,Sekine O,Sunagawa A,Kawamura M,Inoue N,Nishio Y,Maegawa H

更新日期：2014-01-17 00:00:00

abstract：:When treated with T3, P19 cells differentiate into ultrastructurally proven cardiac myocytes and express the cardiac ventricular specific marker ventricular myosin light chain 2V. This differentiation is irreversibly induced in culture during the first 48 hrs of exposure to T3. We studied the binding of P19-indigenous...

journal_title：Biochemical and biophysical research communications

authors： Rodriguez ER,Tan CD,Onwuta US,Parrillo JE

更新日期：1994-12-30 00:00:00

abstract：:Immunoblotting studies on mitochondria prepared from the striata of patients who died of Parkinson's disease were performed using specific antisera against Complexes I, III and IV. In 4 out of 5 patients with Parkinson's disease, the 30-, 25- and 24-kDa subunits of Complex I were moderately to markedly decreased. No c...

journal_title：Biochemical and biophysical research communications

authors： Mizuno Y,Ohta S,Tanaka M,Takamiya S,Suzuki K,Sato T,Oya H,Ozawa T,Kagawa Y

更新日期：1989-09-29 00:00:00

abstract：:Elevations in intracellular Ca(2+) ([Ca(2+)](i)) initiate insulin secretion from pancreatic beta-cells, but the secretory responses become rapidly desensitised to maintained elevations in [Ca(2+)](i). We have investigated the mechanisms underlying the Ca(2+) desensitization of insulin secretion using electrically perm...

journal_title：Biochemical and biophysical research communications

authors： Harris TE,Persaud SJ,Squires PE,Jones PM

更新日期：2000-04-21 00:00:00

abstract：:The aim of current study was to elucidate whether aquaporin-9 (AQP9) expression was involved in the progression of bone loss induced by microgravity. We used the hind-limb suspension (HLS) mice model to simulate microgravity and induce bone loss. It was found that HLS exposure decreased femur bone mineral density (BMD...

journal_title：Biochemical and biophysical research communications

authors： Bu G,Shuang F,Wu Y,Ren D,Hou S

更新日期：2012-03-23 00:00:00

abstract：:Breast cancer anti-estrogen resistance 3 (BCAR3) is an SH2-containing signal transducer and is implicated in tumorigenesis of breast cancer cells. In this study, we found that BCAR3 mediates the induction of ERK activation and DNA synthesis by insulin, but not by IGF-1. Specifically, the SH2 domain of BCAR3 is involve...

journal_title：Biochemical and biophysical research communications

authors： Oh MJ,Yi SJ,Kim HS,Kim JH,Jeong YH,van Agthoven T,Jhun BH

更新日期：2013-11-29 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) have emerged as critical regulators of the progression of human cancers, including colorectal cancer (CRC). The study of genome-wide lncRNA expression patterns in metastatic CRC could provide novel mechanism underlying CRC carcinogenesis. In here, we determined the lncRNA expression prof...

journal_title：Biochemical and biophysical research communications

authors： Shen B,Yuan Y,Zhang Y,Yu S,Peng W,Huang X,Feng J

更新日期：2017-06-17 00:00:00

abstract：:Streptococcus pneumoniae is a pathogenic bacterium that can cause severe invasive diseases, such as pneumonia, otitis media and meningitis. The pro-inflammatory cytokine, IL-1β, has been reported to play important role in host defense against S. pneumoniae. The mechanism of IL-1β maturation and secretion in macrophage...

journal_title：Biochemical and biophysical research communications

authors： Zhang T,Du H,Feng S,Wu R,Chen T,Jiang J,Peng Y,Ye C,Fang R

更新日期：2019-06-04 00:00:00

abstract：:Naringenin is a flavanone compound that alters critical cellular processes such as cell multiplication, glucose uptake, and mitochondrial activity. In this study, we used the social amoeba, Dictyostelium discoideum, as a model system for examining the cellular processes and signaling pathways affected by naringenin. W...

journal_title：Biochemical and biophysical research communications

authors： Misty R,Martinez R,Ali H,Steimle PA

更新日期：2006-06-23 00:00:00

abstract：:The Na(+)/K(+) ATPase generates the Na(+) and K(+) concentration gradients across the plasma membrane and is thus essential for cellular electrolyte homeostasis, cell membrane potential and cell volume maintenance. A powerful regulator of Na(+)/K(+) ATPase is the serum- and glucocorticoid-inducible kinase 1 (SGK1). Th...

journal_title：Biochemical and biophysical research communications

authors： Hosseinzadeh Z,Schmid E,Shumilina E,Laufer S,Borst O,Gawaz M,Lang F

更新日期：2014-09-26 00:00:00

abstract：:This study investigated regulation of autophagy in slow-twitch soleus and fast-twitch plantaris muscles in fasting-related atrophy. Male Fischer-344 rats were subjected to fasting for 1, 2, or 3 days. Greater weight loss was observed in plantaris muscle than in soleus muscle in response to fasting. Western blot analys...

journal_title：Biochemical and biophysical research communications

authors： Ogata T,Oishi Y,Higuchi M,Muraoka I

更新日期：2010-03-26 00:00:00

abstract：:The tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL or Apo2L) and its receptors are members of the tumor necrosis factor superfamily. TRAIL triggers apoptosis by binding to its two proapoptotic receptors DR4 and DR5, a process which is negatively regulated by binding of TRAIL to its two decoy rece...

journal_title：Biochemical and biophysical research communications

authors： Sun SY,Yue P,Zhou JY,Wang Y,Choi Kim HR,Lotan R,Wu GS

更新日期：2001-01-26 00:00:00

abstract：:Regulation of the cAMP-elevating effects of isoproterenol and forskolin in cardiac myocytes by treatments that cause increases in cAMP. We have found that elevations in cyclic AMP (cAMP) have long-term effects on both the beta-adrenergic receptor and adenylyl cyclase in cultured chick ventricular myocytes. Pretreatmen...

journal_title：Biochemical and biophysical research communications

authors： Cui H,Green RD

更新日期：2003-07-18 00:00:00

abstract：:Reoxidation of the reduced insulin A and B chains in 8 M urea leads to a recovery of native insulin of 2-7% whereas in 6 M guanidine or 0.5 mM dodecylsulfate very little, if any, resynthesis of insulin could be detected. Considering all the possibilities of different oligomeric forms containing one or both of the chai...

journal_title：Biochemical and biophysical research communications

authors： Qian YQ,Tsou CL

更新日期：1987-07-31 00:00:00

abstract：:The complex of immobilized human plasminogen (iHPlg) and streptokinase (SK) has a lower catalytic activity in hydrolysis of plasmin-specific substrate S-2251 with a kcat/Km of 0.005 microM-1 x s-1, compared to 0.031 microM-1 x s-1 of immobilized human plasmin (iHPlm) and 0.078 microM-1 x s-1 of HPlm. The SK in the com...

journal_title：Biochemical and biophysical research communications

authors： Shi GY,Chang BI,Wu DH,Wu HL

更新日期：1993-08-31 00:00:00

abstract：:MicroRNA let-7i is up-regulated in T cells from patients with Ankylosing Spondylitis (AS). In this study, we investigated the role of let-7i in T cells survival. Our results demonstrated down-regulation of insulin-like growth factor-1 receptor (IGF1R) in T cells from patients with AS. Luciferase reporter assay suggest...

journal_title：Biochemical and biophysical research communications

authors： Hou C,Zhu M,Sun M,Lin Y

更新日期：2014-10-31 00:00:00

abstract：:We linked a 3.3 kb fragment containing the human gamma-globin gene together with a 5.5 kb fragment containing the human beta-globin gene. This construct was introduced into the germ line of mice. Analysis of the resulting transgenic mice showed that expression of the human gamma- and beta-globin genes were regulated a...

journal_title：Biochemical and biophysical research communications

authors： Katsube T,Tojo H,Sakaguchi T,Fukumaki Y

更新日期：1993-07-15 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPAR-gamma) decreases the growth of certain cancer cells. In the present study, we found that six different human pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-2, HPAF-II, MIA PaCa-2, and PANC-1) expressed PPAR-gamma m-RNA and synthesized the protein. The endogen...

journal_title：Biochemical and biophysical research communications

authors： Eibl G,Wente MN,Reber HA,Hines OJ

更新日期：2001-09-21 00:00:00

abstract：:The α1,3/4-fucosyltransferases (FUT) subfamily are key enzymes in cell surface antigen synthesis during various biological processes. A novel role of FUTs in tumorigenesis has been discovered recently, however, the underlying mechanism remains largely unknown. Here, we characterized FUT6, a member of α1,3/4-FUT subfam...

journal_title：Biochemical and biophysical research communications

authors： Guo Q,Guo B,Wang Y,Wu J,Jiang W,Zhao S,Qiao S,Wu Y

更新日期：2012-01-06 00:00:00

abstract：:Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its eff...

journal_title：Biochemical and biophysical research communications

authors： Saijo S,Nagata K,Nakano Y,Tobe T,Kobayashi Y

更新日期：2005-09-09 00:00:00

abstract：:An important role for the Rel/NF-kappaB family of transcription factors in the differentiation process of dendritic cells (DC) and macrophages (MAC) was recently suggested by a number of mouse knockout studies but only little information is available for defined populations of human cells. To investigate the role of i...

journal_title：Biochemical and biophysical research communications

authors： Ammon C,Mondal K,Andreesen R,Krause SW

更新日期：2000-02-05 00:00:00

abstract：:Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

journal_title：Biochemical and biophysical research communications

authors： Aster JC,Brinn K,Maisel H,Brewer GJ

更新日期：1985-02-15 00:00:00

abstract：:Hammerhead ribozymes may be of great therapeutic importance, since they can, in theory, be developed to cleave any undesired target RNA. In order to design ribozymes which are stable against endogenous RNases, we incorporated 2'-acetamido-2'-deoxynucleosides in the catalytic core of a hammerhead ribozyme. The 2'-aceta...

journal_title：Biochemical and biophysical research communications

authors： Hendrix C,Mahieu M,Anné J,Van Calenbergh S,Van Aerschot A,Content J,Herdewijn P

更新日期：1995-05-05 00:00:00