当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > DNA chain unwinding and annealing reactions of lipocortin (annexin) I heterotetramer: regulation by Ca(2+) and Mg(2+).

DNA chain unwinding and annealing reactions of lipocortin (annexin) I heterotetramer: regulation by Ca(2+) and Mg(2+).

Abstract：

:Lipocortin I-S100 (calcyclin) heterotetramer exhibited ATPase activity in the presence of dsDNA but not ssDNA. To demonstrate its helicase activity, an 80-mer polynucleotide complementary to the replication origin of M13mp18 was synthesized, and the oligonucleotide, (dC)(20), was ligated to either its 5'- or 3'- end for binding to lipocortin. Lipocortin I heterotetramer displaced chains of the partially Y-shaped duplexes with a dC-tail at either the 5'- or 3'- end. The chain displacement required ATP and Mg(2+). Nonhydrolyzable ATP analogues were not effective. Lipocortin I heterotetramer also catalyzed annealing of the polynucleotides to M13mp18. Ca(2+) and phospholipids but not ATP and Mg(2+) were essential for this reaction. Since the chain displacing and annealing reactions were inhibited by monospecific anti-lipocortin I or anti-S100 antibodies, the present observations suggest that the lipocortin I heterotetramer regulates unwinding and annealing of DNA by Mg(2+) (plus ATP) and Ca(2+) (and phospholipids), respectively.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hirata A,Hirata F

doi

10.1006/bbrc.2002.6422

subject

Has Abstract

pub_date

2002-02-22 00:00:00

pages

205-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X02964223

journal_volume

291

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • The β-domain of cluster 2b streptokinase is a major determinant for the regulation of its plasminogen activation activity by cellular plasminogen receptors.

    abstract：:Cluster 2b streptokinase (SK2b), secreted by invasive skin-trophic strains of Streptococcus pyogenes (GAS), is a human plasminogen (hPg) activator that optimally functions when human plasma hPg is bound, via its kringle-2 domain, to cognizant bacterial cells through the a1a2 domain of the major cellular hPg receptor, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.109

    authors： Zhang Y,Mayfield JA,Ploplis VA,Castellino FJ

    更新日期：2014-02-21 00:00:00

  • Mitochondria and caspases in induced apoptosis in human luteinized granulosa cells.

    abstract：:Apoptosis occurs as a physiologic process in the ovarian life cycle. Staurosporine, a protein kinase inhibitor, is reported to induce apoptosis. Here, we hypothesize that staurosporine will induce apoptosis in human luteinized granulosa cells and that mitochondria and the caspase cascade participate in this process. L...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2321

    authors： Khan SM,Dauffenbach LM,Yeh J

    更新日期：2000-03-16 00:00:00

  • FoxA1 as a lineage-specific oncogene in luminal type breast cancer.

    abstract：:The forkhead transcription factor FoxA1 is thought to be involved in mammary tumorigenesis. However, the precise role of FoxA1 in breast cancer development is controversial. We examined expression of FoxA1 in 35 human breast cancer cell lines and compared it with that of ErbB2, a marker of poor prognosis in breast can...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.064

    authors： Yamaguchi N,Ito E,Azuma S,Honma R,Yanagisawa Y,Nishikawa A,Kawamura M,Imai J,Tatsuta K,Inoue J,Semba K,Watanabe S

    更新日期：2008-01-25 00:00:00

  • Engineered peptides corresponding to segments of the H3 domain of syntaxin inhibit insulin release both in intact and permeabilized mouse pancreatic beta cells.

    abstract：:Syntaxin is one of the proteins involved in the exocytotic event through sequential binding to specific proteins, including SNAP25 and synaptobrevin. In a previous work in digitonin-permeabilized beta cells, we characterized the functional role of two segments: synA and synB of the H3 domain of syntaxin. As a continua...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8923

    authors： Martin F,Salinas E,Barahona F,Vázquez J,Soria B,Reig JA

    更新日期：1998-07-09 00:00:00

  • The transcriptional efficiency of clustered tRNA genes is affected by their position within the cluster.

    abstract：:The transcription of a mouse genomic segment containing four tRNA genes, coding for a tRNA(Ala), a tRNA(Ile), a tRNA(Pro) and a tRNA(Lys), has been studied in a HeLa cell extract, demonstrating that differences among their transcriptional efficiencies are evident using as templates either the natural cluster or an equ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90524-9

    authors： Russo T,Oliva A,Duilio A,Ammendola R,Costanzo F,Zannini M,Cimino F

    更新日期：1987-12-31 00:00:00

  • Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors.

    abstract：:We have cloned the cDNA encoding human PICK1 (protein interacting with C kinase 1), a PDZ domain-containing protein of 415 amino acids, and also identified the Drosophila homologue by search of the databank. Northern blot analysis shows a single mRNA of about 2.0 kb ubiquitously expressed in human tissues. Although PI...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1932

    authors： Takeya R,Takeshige K,Sumimoto H

    更新日期：2000-01-07 00:00:00

  • Can microbes be patented?

    abstract：:Native microorganisms in their original form cannot be patented. However, microbes like yeasts, bacteria, protozoa, unicellular algae, fungi, actinomycetes and viruses can be patented if they have been genetically modified. The process and the product obtained can also be patented. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 信件

    doi：10.1016/j.bbrc.2012.11.032

    authors： Cameotra SS

    更新日期：2013-01-04 00:00:00

  • A metal-dependent form of protein phosphatase 2A.

    abstract：:Highly purified bovine heart protein phosphatase 2A catalytic subunit lost virtually all of its activity during storage at -70 degrees. When the enzyme was preincubated with Co2+, over 35% of the original activity was restored. Freshly prepared protein phosphatase 2A purified from bovine heart was stimulated at least ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1334

    authors： Cai L,Chu Y,Wilson SE,Schlender KK

    更新日期：1995-03-08 00:00:00

  • Role of protein kinase C-alpha in activation of ecto-5'-nucleotidase in the preconditioned canine myocardium.

    abstract：:We have reported that activation of protein kinase C (PKC) increases ecto-5'-nucleotidase activity, which may contribute to the infarct size-limiting effect of ischemic preconditioning. Since we have reported that Ca(2+)- and phospholipid-sensitive PKC is activated due to ischemic preconditioning, we further tested 1)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7445

    authors： Kitakaze M,Funaya H,Minamino T,Node K,Sato H,Ueda Y,Okuyama Y,Kuzuya T,Hori M,Yoshida K

    更新日期：1997-10-09 00:00:00

  • Oxidized low-density lipoprotein stimulates nitric oxide release by rabbit aortic endothelial cells.

    abstract：:Incubation of rabbit aortic endothelial cells (RAEC) with oxidized low-density lipoprotein (LDL) for 16 h resulted in stimulation of calcium uptake and increased release of nitric oxide (NO) by these cells. Accompanying inhibition of protein tyrosine phosphatase (PTP) activity in these cells was also observed. Convers...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1177

    authors： Fries DM,Penha RG,D'Amico EA,Abdalla DS,Monteiro HP

    更新日期：1995-02-06 00:00:00

  • Inhibition of wortmannin activities by amino compounds.

    abstract：:Wortmannin caused normal and strong inhibition on catecholamine secretion from bovine adrenal chromaffin cells and in vitro phosphoinositide 3-kinase activity in NaCl-, Na isethionate-, choline Cl-, Na acetate-, and N-acetyl glycine-based media. However, brief preincubation of wortmannin with the media containing amin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.200

    authors： Isosaki M

    更新日期：2004-11-26 00:00:00

  • Anticancer activity of arborinine from Glycosmis parva leaf extract in human cervical cancer cells.

    abstract：:Glycosmis parva is a small shrub found in Thailand. Ethyl acetate (EtOAc) extract from its leaves has been shown to exert anticancer effects in vitro; however, the compound responsible for this activity has not been isolated and characterized. In this study, we demonstrate that arborinine, a major acridone alkaloid in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.04.175

    authors： Piboonprai K,Khumkhrong P,Khongkow M,Yata T,Ruangrungsi N,Chansriniyom C,Iempridee T

    更新日期：2018-06-12 00:00:00

  • Involvement of Gi/o in the PAR-4-induced NO production in endothelial cells.

    abstract：:We investigated the involvement of G(i/o) protein in NO production following the activation of proteinase-activated receptor-4 (PAR-4) in cultured bovine aortic endothelial cells. AYPGKF-NH(2) (PAR-4 activating peptide), thrombin, and ionomycin induced a concentration-dependent NO production, with the maximal producti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.165

    authors： Momota F,Hirano K,Hirano M,Nishimura J,Kanaide H

    更新日期：2006-04-07 00:00:00

  • Caffeine lengthens circadian rhythms in mice.

    abstract：:Although caffeine alters sleep in many animals, whether or not it affects mammalian circadian clocks remains unknown. Here, we found that incubating cultured mammalian cell lines, human osteosarcoma U2OS cells and mouse fibroblast NIH3T3 cells, with caffeine lengthened the period of circadian rhythms. Adding caffeine ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.049

    authors： Oike H,Kobori M,Suzuki T,Ishida N

    更新日期：2011-07-08 00:00:00

  • Evidence for constitutive dimerization of niacin receptor subtypes.

    abstract：:The recently deorphanized niacin receptor subtypes NIACR1 (GPR109A) and NIACR2 (GPR109B) play an essential role in the regulation of metabolic processes and immune reactions. Both receptors belong to the G-protein-coupled receptor (GPCR) family, whose members have traditionally been treated as monomeric entities, but ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.011

    authors： Mandrika I,Petrovska R,Klovins J

    更新日期：2010-04-30 00:00:00

  • Molecular architecture of CTCFL.

    abstract：:The CTCF-like protein, CTCFL, is a DNA-binding factor that regulates the transcriptional program of mammalian male germ cells. CTCFL consists of eleven zinc fingers flanked by polypeptides of unknown structure and function. We determined that the C-terminal fragment predominantly consists of extended and unordered con...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.146

    authors： Campbell AE,Martinez SR,Miranda JJ

    更新日期：2010-06-04 00:00:00

  • Overexpression of adenine nucleotide translocase reduces Ca2+ signal transmission between the ER and mitochondria.

    abstract：:The adenine nucleotide translocase (ANT), besides transferring ATP from the mitochondrial matrix to the rest of the cell, has also been proposed to be involved in mitochondrial permeability transition (MPT), and accordingly in mitochondrial Ca2+ homeostasis. In order to assess the role of ANT in Ca2+ signal transmissi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.07.072

    authors： Wieckowski MR,Szabadkai G,Wasilewski M,Pinton P,Duszyński J,Rizzuto R

    更新日期：2006-09-22 00:00:00

  • Insulin/NFκB protects against ischemia-induced necrotic cardiomyocyte death.

    abstract：:In the heart, insulin controls key functions such as metabolism, muscle contraction and cell death. However, all studies have been focused on insulin action during reperfusion. Here we explore the cardioprotective action of this hormone during ischemia. Rat hearts were perfused ex vivo with an ischemia/reperfusion Lan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.171

    authors： Díaz A,Humeres C,González V,Gómez MT,Montt N,Sanchez G,Chiong M,García L

    更新日期：2015-11-13 00:00:00

  • Vicinal thiols are involved in inositol 1,2,3,5,6-pentakisphosphate 5-phosphatase activity from fetal calf thymus.

    abstract：:Inositol 1,2,3,5,6-pentakisphosphate (Ins(1,2,3,5,6)P5) 5-phosphatase present in fetal calf thymus has been partially purified. This enzyme was inhibited dose-dependently by different thiol modifiers like N-ethylmaleimide (NEM), p-chloromercuribenzene sulfonate (PCMBS), diamide, and phenylarsine oxide (PAO). The inhib...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7629

    authors： Bandyopadhyay U,Kaiser T,Rudolf MT,Schultz C,Guse AH,Mayr GW

    更新日期：1997-11-07 00:00:00

  • Decrease of NADH in HeLa cells in the presence of transferrin or ferricyanide.

    abstract：:The short-term incubation of HeLa cells in the presence of diferric transferrin or ferricyanide, which are reduced externally by the transplasma membrane reductase, produces a stoichiometric decrease in NADH and increase in NAD+, which is stimulated by insulin. The NADP/NADPH ratio does not change during 15 min incuba...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90949-6

    authors： Navas P,Sun IL,Morré DJ,Crane FL

    更新日期：1986-02-26 00:00:00

  • C57BL/6JRj mice are protected against diet induced obesity (DIO).

    abstract：:The C57BL/6 (B6) is one of the oldest inbred mouse strains. It has been widely used as control strain in metabolism research for many decades. Preliminary data from our lab indicated that C57BL/6JRj mice are not responding to diet induced obesity. Therefore, the aim of this study was to compare the two different B6 su...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.008

    authors： Kern M,Knigge A,Heiker JT,Kosacka J,Stumvoll M,Kovacs P,Blüher M,Klöting N

    更新日期：2012-01-13 00:00:00

  • Different roles of GPR120 and GPR40 in the acquisition of malignant properties in pancreatic cancer cells.

    abstract：:Free fatty acids (FFAs) act as extracellular signaling molecules through binding to G-protein-coupled FFA receptors (FFARs). GPR120 and GPR40 are identified as FFARs for medium- and long-chain fatty acids. In the present study, we investigated roles of GPR120 and GPR40 in cellular functions of pancreatic cancer PANC-1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.08.050

    authors： Fukushima K,Yamasaki E,Ishii S,Tomimatsu A,Takahashi K,Hirane M,Fukushima N,Honoki K,Tsujiuchi T

    更新日期：2015-09-25 00:00:00

  • Retroviral suicide vector does not inhibit neointimal growth in a porcine coronary model of restenosis.

    abstract：:We attempted to attenuate neointimal formation following vascular injury using a retroviral suicide vector. Epicardial coronary arteries of adult miniature swine were injured by deployment of oversized tantalum stents. One week later, injured segments were exposed to packaged retroviral constructs with or without the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1157

    authors： Barbee RW,Stapleton DD,Madras DE,Ré RN,Murgo JP,Davenport WD,Giardina J,Cook JL

    更新日期：1995-02-06 00:00:00

  • Biochemical analysis of Lgt3, a glycosyltransferase of the bacterium Moraxella catarrhalis.

    abstract：:The lipooligosaccharide (LOS) of Moraxella catarrhalis is unusual in that it lacks heptose. The sugar linking oligosaccharide to Lipid A is a trisubstituted glucose. A single enzyme, Lgt3, is suggested to trisubstitute this core sugar. The lgt3 gene encodes two distinct domains with high similarity to glucosyltransfer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.02.028

    authors： Faglin I,Tiralongo J,Wilson JC,Collins PM,Peak IR

    更新日期：2010-03-19 00:00:00

  • Effects of inhibiting transglutaminase during egg fertilization and development.

    abstract：:Transglutaminase inhibitor (1,3,4,5-tetramethyl-2-[(2-oxopropyl)thio]imidazolium chloride or L-682777) affected the appearance of the fertilization envelope (FE) and subsequent development in Sphaerechinus granularis and Arbacia punctulata in a concentration-dependent manner. Abnormalities were first visible in formin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2702

    authors： Cariello L,Zanetti L,Lorand L

    更新日期：1994-11-30 00:00:00

  • PRS3 encoding an essential subunit of yeast proteasomes homologous to mammalian proteasome subunit C5.

    abstract：:We found by computer analysis that a putative yeast proteasome subunit gene named PRS3 that encodes a protein very similar to subunit C5 of rat and human proteasomes is located immediately 3' to the ERD2 gene of Saccharomyces cerevisiae. The similarity of the primary structures of the two suggests that this subunit ma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91753-d

    authors： Lee DH,Tanaka K,Tamura T,Chung CH,Ichihara A

    更新日期：1992-01-31 00:00:00

  • Exosomes expressing carbonic anhydrase 9 promote angiogenesis.

    abstract：:Exosomes or microvesicles that are secreted from cells are considered to play important roles in tumor microenvironment. Carbonic anhydrase 9 (CA9), which is induced by hypoxia-inducible factor 1 (HIF1) in response to hypoxia, is overexpressed in many types of cancer including renal cell carcinoma (RCC). We examined t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.08.107

    authors： Horie K,Kawakami K,Fujita Y,Sugaya M,Kameyama K,Mizutani K,Deguchi T,Ito M

    更新日期：2017-10-21 00:00:00

  • GP2/THP gene family of self-binding, GPI-anchored proteins forms a cluster at chromosome 7F1 region in mouse genome.

    abstract：:We investigated the genomic organization of pancreatic zymogen granule membrane-associated protein GP2, a GPI-anchored protein exhibiting self-aggregation at acidic pH, in order to construct a gene-knockout mouse. Cloning and analysis of lambda clones encoding GP2 from 129 Svj mouse genomic DNA libraries showed that t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.197

    authors： Kobayashi K,Yanagihara K,Ishiguro K,Fukuoka S

    更新日期：2004-09-17 00:00:00

  • Detection of purine cytosine permease of S. cerevisiae: use of antibodies against a synthetic peptide corresponding to a predicted sequence in the N-terminal domain of the protein.

    abstract：:A synthetic peptide, selected in the predicted N-terminal amino-acid sequence of the purine cytosine permease (gene FCY2), linked to albumins proved a remarkably good immunogen in rabbits. In ELISA, sera reacted with the synthetic peptide and with specific proteins of plasma-membrane-enriched fractions of mutant Sacch...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2489

    authors： Grandier-Vazeille X,Neaud V,Geoffre S

    更新日期：1993-12-15 00:00:00

  • Structural modifications of interleukin-2 at positions 47 and 65.

    abstract：:Interleukin-2 (IL-2) is a cytokine essential for the growth and proliferation of T-cells. The purpose of this research was to study the effects of altering the "kink" caused by Pro in an alpha-helix of the protein. The Pro-47 residue was chosen because it was originally, but mistakenly, traced to the kink of an alpha-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6453

    authors： Lee BL,Ciardelli TL

    更新日期：1997-04-17 00:00:00