Nucleotide triphosphatase activity of the N-terminal nucleotide-binding domains of the multidrug resistance proteins P-glycoprotein and MRP1.

abstract：:A gene encoding the human immunodeficiency virus-1 (HIV-1) TAT protein was chemically synthesized and expressed in HeLa cells and in a cell-free system. To facilitate both the assembly of the synthetic gene and further mutagenesis and gene fusion studies, several unique restriction endonuclease cleavage sites were inc...

journal_title：Biochemical and biophysical research communications

authors： Ferguson BQ,Friedland BK,Hendrickson E,Strehl L,Petteway SR,Jonak GJ

更新日期：1989-05-15 00:00:00

abstract：:The repair of point mutations in hprt gene by single-stranded oligonucleotides represents a model to test targeted nucleotide exchange. We studied the concurrent nucleotide exchange of two or three nucleotides in the hprt deficient hamster cell line V79-151. The used oligonucleotides resulted in mismatches at two (151...

journal_title：Biochemical and biophysical research communications

authors： Kenner O,Lutomska A,Speit G,Vogel W,Kaufmann D

更新日期：2004-09-03 00:00:00

abstract：:The processing of human gastric procathepsin E to its mature form, cathepsin E, was studied at pH 3.5. The results revealed the autocatalytic and apparently one-step conversion of procathepsin E to cathepsin E within 10 min of incubation at 14 degrees C under the conditions used. Analyses of the amino acid sequences o...

journal_title：Biochemical and biophysical research communications

authors： Athauda SB,Takahashi T,Kageyama T,Takahashi K

更新日期：1991-02-28 00:00:00

abstract：:A protein which inhibits the prophenoloxidase----phenoloxidase (EC 1.14.18.1) proteolytic activation in hemocyte extracts of Locusta migratoria was isolated from the plasma of the same insect and partially characterized. It shows a molecular weight of 14,000, an inhibiting activity toward the cascade system in the ins...

journal_title：Biochemical and biophysical research communications

authors： Brehélin M,Boigegrain RA,Drif L,Coletti-Previero MA

更新日期：1991-09-16 00:00:00

abstract：:Cilostazol is a drug licensed for the treatment of intermittent claudication. Its main action is to elevate intracellular levels of cyclic monophosphate (cAMP) by inhibiting the activity of type III phosphodiesterase, a cAMP-degrading enzyme. The effects of cilostazol on fatty acid oxidation (FAO) are as yet unknown. ...

journal_title：Biochemical and biophysical research communications

authors： Wang B,Zhu L,Sui S,Sun C,Jiang H,Ren D

更新日期：2014-05-09 00:00:00

abstract：:In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat alpha 1(I) procollagen promoter with a Kd of 0.1-0.2 microM. The formation of triplexes by the triplex forming oligoribonucleotide ...

journal_title：Biochemical and biophysical research communications

authors： Ririe SS,Guntaka RV

更新日期：1998-08-10 00:00:00

abstract：:Granulocytin, a C-type lectin from Sarcophaga peregrina (flesh fly), stimulated glucose consumption and cytokine production by the mouse macrophage-like cell line J774.1. When J774.1 cells were pretreated with tunicamycin, their granulocytin-dependent TNF-alpha production was greatly reduced. These results suggest tha...

journal_title：Biochemical and biophysical research communications

authors： Fujita Y,Homma KJ,Natori S

更新日期：2000-09-07 00:00:00

abstract：:Human erythrocyte G6PD activity was measured in more than 500 subjects in Isfahan, Iran, and the percent of enzyme deficiency for males and females are reported. Some properties of the abnormal enzyme is compared with its normal counterpart. Apparent Km values of glucose 6-phosphate for the variant and normal enzymes ...

journal_title：Biochemical and biophysical research communications

authors： Haghighi B,Suzangar M,Yazdani A,Mehnat M

更新日期：1985-11-15 00:00:00

abstract：:The effect of eicosapentaenoic acid (EPA) on natural killer (NK) cell activity of human lymphocytes was examined. The addition of an emulsion of trieicosapentaenoyl-glycerol (EPA-TG) emulsified with purified phosphatidylcholine from krill to a cytotoxicity assay system resulted in a marked depression of NK activity. T...

journal_title：Biochemical and biophysical research communications

authors： Yamashita N,Yokoyama A,Hamazaki T,Yano S

更新日期：1986-08-14 00:00:00

abstract：:The purpose of the present study was to investigate the effect of acute exercise on lipolysis via coordination of hormone-sensitive lipase (HSL) and scaffold proteins, i.e., perilipin A and comparative gene identification-58 (CGI-58), in rat primary adipocytes. Glycerol release was significantly elevated immediately (...

journal_title：Biochemical and biophysical research communications

authors： Ogasawara J,Nomura S,Rahman N,Sakurai T,Kizaki T,Izawa T,Ishida H,Haga S,Ohno H

更新日期：2010-09-10 00:00:00

abstract：:Tankyrases (TNKS and TNKS2) are enzymes that catalyze poly-ADP-ribosylation (PARsylation) of their target proteins. Tankyrase-mediated PARsylation plays critical regulatory roles in cell signaling, particularly in the Wnt/β-catenin pathway. The sterile alpha motif (SAM) domain in tankyrases mediates their oligomerizat...

journal_title：Biochemical and biophysical research communications

authors： Fan C,Yarravarapu N,Chen H,Kulak O,Dasari P,Herbert J,Yamaguchi K,Lum L,Zhang X

更新日期：2018-09-10 00:00:00

abstract：:Identification of TNF alpha receptors and their regulation by the endocrine system were examined in testicular Sertoli cells. By using cross-linking experiments as well as northern blotting analysis, we detected the p55 receptor type (MW: 55 kDa, mRNA: 2.3 kb), but not the p75 TNF alpha receptor type. Furthermore, we ...

journal_title：Biochemical and biophysical research communications

authors： Mauduit C,Besset V,Caussanel V,Benahmed M

更新日期：1996-07-25 00:00:00

abstract：:This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells. We demonstrate that MEFvin(-/-) cells transfected with the eGFP-vinculin mutant A50I (talin-binding-deficient-vinculin in a constitutively closed conformation) show 2-fold lower stiffness and focal adhesion den...

journal_title：Biochemical and biophysical research communications

authors： Diez G,Auernheimer V,Fabry B,Goldmann WH

更新日期：2011-03-04 00:00:00

abstract：:Excessive cellular cholesterol is transported to the liver by a pathway called 'reverse cholesterol transport.' Scavenger receptor class B, type I (SR-BI) mediates cholesterol uptake in the liver. Polyunsaturated fatty acids, known to activate peroxisome proliferator-activated receptor (PPAR), have been reported to in...

journal_title：Biochemical and biophysical research communications

authors： Malerød L,Sporstøl M,Juvet LK,Mousavi A,Gjøen T,Berg T

更新日期：2003-06-06 00:00:00

abstract：:Nanosecond and steady fluorescence techniques have been employed to study the interaction of retinol with protein disulfide isomerase (PDI). Retinol binds tightly to PDI; and the rotational correlation time (θ = 36 ns) corresponds to a monomeric subunit of 55 kDa. The enzyme does not undergo aggregation in the p...

journal_title：Biochemical and biophysical research communications

authors： Churchich JE

更新日期：1999-07-22 00:00:00

abstract：:We have previously reported that endothelin-1 (ET-1) stimulates interleukin-6 (IL-6), a potent bone resorptive agent, through p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the involvement of Rho-kinase in the ET-1-stimulated I...

journal_title：Biochemical and biophysical research communications

authors： Tokuda H,Hanai Y,Matsushima-Nishiwaki R,Yamauchi J,Doi T,Harada A,Takai S,Kozawa O

更新日期：2007-11-03 00:00:00

abstract：:Cigarette smoke (CS) is the principal cause of pulmonary inflammatory response. IL-28 (IFN-λ) is a novel group of class II cytokines targeting the epithelial cells and IL-28 responses prominent in lungs can exert important immunomodulatory effects. We tested the hypothesis that IL-28B may modulate the lung inflammatio...

journal_title：Biochemical and biophysical research communications

authors： Qiu C,Li Y,Zhou M,Liu J,Li M,Wu Y,Xu D,Li M

更新日期：2014-05-09 00:00:00

abstract：:A cDNA for a GnRH receptor (mtGnRH-R) was obtained from a mouse gonadotropic pituitary cell line (alpha T3-1) by expression cloning. This full-length cDNA was subsequently used as a probe to clone a rat pituitary GnRH receptor (rGnRH-R). The two receptors differ by 13 amino acids and are 100% identical to those recent...

journal_title：Biochemical and biophysical research communications

authors： Perrin MH,Bilezikjian LM,Hoeger C,Donaldson CJ,Rivier J,Haas Y,Vale WW

更新日期：1993-03-31 00:00:00

abstract：:α-Dystroglycan is an extracellular adhesion protein that is known to interact with different ligands. The interaction is thought to stabilize the integrity of the plasma membrane. The N-terminal part of α-dystroglycan may be proteolytically processed to generate a small 38 kDa protein (α-DG-N). The physiological signi...

journal_title：Biochemical and biophysical research communications

authors： Hesse C,Johansson I,Mattsson N,Bremell D,Andreasson U,Halim A,Anckarsäter R,Blennow K,Anckarsäter H,Zetterberg H,Larson G,Hagberg L,Grahn A

更新日期：2011-09-02 00:00:00

abstract：:A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between ...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Oida S,Ichijo H,Iimura T,Maruoka Y,Amagasa T,Sasaki S

更新日期：1994-10-14 00:00:00

abstract：:Primary in vitro immunization of peripheral blood lymphocytes has been studied for the production of human monoclonal antibodies. Depletion of a lysosome-rich cell population, containing large granular lymphocytes, monocytes, and a subset of T suppressor cells, was shown to be crucial before the cells could be immuniz...

journal_title：Biochemical and biophysical research communications

authors： Borrebaeck CA,Danielsson L,Möller SA

更新日期：1987-11-13 00:00:00

abstract：:Apoptosis is a well defined physiological process characterised by many specific features including DNA fragmentation and protease activation. Cell membrane-associated changes such as the altered exposure of phosphatidylserine and glycosylation patterns have also been described. We investigate here the change in expos...

journal_title：Biochemical and biophysical research communications

authors： Russell L,Waring P,Beaver JP

更新日期：1998-09-18 00:00:00

abstract：:Hepatitis delta virus (HDV) is a human pathogen causing fulminant hepatitis and liver cirrhosis. HDV has a circular single-stranded RNA genome, which encodes only a single protein, hepatitis delta antigen (HDAg), from the antigenomic strand. Although the functional roles of HDAg have been extensively studied, the mole...

journal_title：Biochemical and biophysical research communications

authors： Hwang SB,Park KJ,Kim YS

更新日期：1998-03-27 00:00:00

abstract：:In several inflammatory conditions such as rheumatoid arthritis or sepsis, the regulatory mechanisms of inflammation are inefficient and the excessive inflammatory response leads to damage to the host. Sirtuins are class III histone deacetylases that modulate the activity of several transcription factors that are impl...

journal_title：Biochemical and biophysical research communications

authors： Fernandes CA,Fievez L,Neyrinck AM,Delzenne NM,Bureau F,Vanbever R

更新日期：2012-04-20 00:00:00

abstract：:The compounds [Pt(IV)(dach)(9-methylguanine)2X2]2+ (X = Cl, OH) have been prepared and structurally characterized. Their existence shows that it is possible to accommodate two purine bases (in a cis configuration) and four other ligands around a Pt(IV) atom. The geometries of these complexes have very different orient...

journal_title：Biochemical and biophysical research communications

authors： Choi HK,Huang SK,Bau R

更新日期：1988-11-15 00:00:00

abstract：:Cytotoxic lymphocytes utilize granule associated serine proteases (granzymes) and perforin to induce apoptosis. Although the importance of granzyme B has been established by gene ablation experiments, biochemical events initiated by the granzyme remain enigmatic. We show here that exposure of Jurkat cells to granzyme ...

journal_title：Biochemical and biophysical research communications

authors： Froelich CJ,Hanna WL,Poirier GG,Duriez PJ,D'Amours D,Salvesen GS,Alnemri ES,Earnshaw WC,Shah GM

更新日期：1996-10-23 00:00:00

abstract：:Due to the structural and functional importance of tight turns, some methods have been proposed to predict gamma-turns, beta-turns, and alpha-turns in proteins. In the past, studies of pi-turns were made, but not a single prediction approach has been developed so far. It will be useful to develop a method for identify...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Xue ZD,Shi XH,Xu J

更新日期：2006-09-01 00:00:00

abstract：:When human ovarian carcinoma cells are challenged with endotoxin, an I kappa B kinase is transiently induced within 3 to 5 min. This enzyme activity causes the hyperphosphorylation and subsequent degradation of I kappa B which allows NF-kappa B to translocate to the nucleus where it activates transcription. When endot...

journal_title：Biochemical and biophysical research communications

authors： Kohler NG,Joly A

更新日期：1997-03-27 00:00:00

abstract：:GTP gamma S-dependent phospholipase D (PLD) activity time-dependently increased during differentiation of rat C6 glioma cells to astrocytic phenotypes induced by dibutyryl cyclic AMP (dbcAMP)/theophylline. The changes in PLD mRNA level were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) method us...

journal_title：Biochemical and biophysical research communications

authors： Yoshimura S,Nakashima S,Ohguchi K,Sakai H,Shinoda J,Sakai N,Nozawa Y

更新日期：1996-08-14 00:00:00

abstract：:The conformational dependence of the interresidue interproton distances in peptides, C alpha H ... Ni + 1 H and NiH ... Ni + 1 H, have been used to identify zones of sterically allowed phi, psi space, where both distances are less than 3A and expected to yield nuclear Overhauser effects (NOEs). L-residues in left-hand...

journal_title：Biochemical and biophysical research communications

authors： Ramakrishnan C,Sukumar M,Balaram P

更新日期：1987-12-31 00:00:00