当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Transposon-independent increase of transcription by the Sleeping Beauty transposase.

Transposon-independent increase of transcription by the Sleeping Beauty transposase.

Abstract：

:When a plasmid encoding the Sleeping Beauty (SB) transposase (pCMV-SB) was cointroduced with luciferase expression plasmid DNA into mouse skeletal muscle at a molar ratio of 4:1, luciferase gene expression was 5 times higher than the expression without pCMV-SB on day 28. This enhancement was not dependent on the presence of transposon (Tn) sequence in luciferase expression plasmid. Southern blot analysis failed to detect luciferase gene insertion into the host genome. Then, expression, a luciferase expression plasmid without Tn, was cointroduced into HeLa cells with or without pCMV-SB. With pCMV-SB, the mRNA amount and the luciferase activity were 1.5 times and 2 times higher, respectively, than without pCMV-SB, even though the cells with pCMV-SB had a smaller copy number of luciferase plasmids than the cells without pCMV-SB. These results suggest that SB transposase enhances the transcription of an exogenous gene regardless of the presence of the Tn sequence.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Masuda K,Yamamoto S,Endoh M,Kaneda Y

doi

10.1016/j.bbrc.2004.03.116

subject

Has Abstract

pub_date

2004-05-07 00:00:00

pages

796-800

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X04006126

journal_volume

317

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Polymorphisms of TNFalpha, IL1beta, and IL1RN genes in chronic obstructive pulmonary disease.

    abstract：:It is recognized that genetic factors play a role in the susceptibility to COPD. COPD is characterized by airflow limitation. Chronic inflammation causes small airway disease and parenchymal destruction, leading to the airflow limitation. Polymorphisms in pro-inflammatory cytokine genes may confer a risk for the devel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.099

    authors： Hegab AE,Sakamoto T,Saitoh W,Nomura A,Ishii Y,Morishima Y,Iizuka T,Kiwamoto T,Matsuno Y,Massoud HH,Massoud HM,Hassanein KM,Sekizawa K

    更新日期：2005-04-22 00:00:00

  • Activity of the isolated HIV RNase H domain and specific inhibition by N-hydroxyimides.

    abstract：:This report describes a procedure to generate enzymatically active, isolated HIV RNase H domain. In contrast to previously described preparations, the RNA cleavage activity of the untagged RNase H domain was surprisingly similar to that of the full-length HIV-RT protein. Signature cleavages at 18 and 9 nucleotides dow...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.061

    authors： Hang JQ,Rajendran S,Yang Y,Li Y,In PW,Overton H,Parkes KE,Cammack N,Martin JA,Klumpp K

    更新日期：2004-04-30 00:00:00

  • Identification of human semaphorin E gene expression in rheumatoid synovial cells by mRNA differential display.

    abstract：:Rheumatoid arthritis (RA) is characterised by chronic inflammation of synovial tissue with aggressive proliferation of synovial cells causing destruction of cartilage and bone. Immunopathological mechanisms, infectious causes and genetic factors have been discussed, but the etiology of the disease has not been underst...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6607

    authors： Mangasser-Stephan K,Dooley S,Welter C,Mutschler W,Hanselmann RG

    更新日期：1997-05-08 00:00:00

  • Malignant transformation of an epithelial cell by v-Src via tv-a-mediated retroviral infection: a new cell model for studying carcinogenesis.

    abstract：:Most human cancers are of epithelial origin, but many cell culture models for the study of cancer-causing genes use fibroblasts. In addition, efficient delivery and stable expression of foreign genes into non-transformed cell lines are often difficult. To address both questions, we here established a non-transformed r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.045

    authors： Fu SL,Huang YJ,Liang FP,Huang YF,Chuang CF,Wang SW,Yao JW

    更新日期：2005-12-16 00:00:00

  • Phorbol ester regulation of the human gamma-glutamyltransferase gene promoter.

    abstract：:In the present study the molecular mechanisms underlying tetradecanoylphorbol-13-acetate (TPA) mediated regulation of the human gamma-glutamyltransferase (GGT) gene were examined. TPA challenge of HeLa cells resulted in an increase of GGT mRNA and enzyme activity. Deletion analysis of the promoter revealed that the -3...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.121

    authors： Daubeuf S,Duvoix A,Wellman-Rousseau M,Diederich M,Visvikis A

    更新日期：2004-01-09 00:00:00

  • Identification of novel initiation sites for human DNA replication around ARSH1, a previously characterized yeast replicator.

    abstract：:Replication of mammalian chromosomes depends on the activation of a large number of origins of DNA replication distributed along the chromosomes. We have focused our attention on a human DNA region, named ARSH1, localized to chromosome 2, that had been previously shown to act as an episomal origin in the yeast Sacchar...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.040

    authors： Hu L,Xu X,Valenzuela MS

    更新日期：2004-01-23 00:00:00

  • In vitro transcription termination activity of the Drosophila mitochondrial DNA-binding protein DmTTF.

    abstract：:DmTTF is a Drosophila melanogaster mitochondrial DNA-binding protein which binds specifically to two homologous non-coding sequences located at the 3' ends of blocks of genes encoded on opposite strands. In order to test whether this protein acts as transcription termination factor, we assayed the capacity of DmTTF to...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.173

    authors： Roberti M,Fernandez-Silva P,Polosa PL,Fernandez-Vizarra E,Bruni F,Deceglie S,Montoya J,Gadaleta MN,Cantatore P

    更新日期：2005-05-27 00:00:00

  • Affinity labeling of delta opioid receptors by an enkephalin-derivative alkylating agent, DSLET-Mal.

    abstract：:Opioid binding properties of Tyr-D-Ser-Gly-Phe-Leu-Thr-NH-NH-Gly-Mal (DSLET-Mal), a novel enkephalin-framed affinity label, was determined in rat brain membranes. In competition studies the ligand showed high affinity for the delta opioid sites, labelled by [(3)H][Ile(5,6)]deltorphin II (K(i) = 8 nM), whereas its bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1682

    authors： Szatmári I,Orosz G,Medzihradszky K,Borsodi A

    更新日期：1999-11-19 00:00:00

  • Effect of retinoic acid on osteocalcin gene expression in human osteoblasts.

    abstract：:It is well known that the expression of osteocalcin, the only recognized osteoblast-specific protein, is regulated by 1 alpha-25-dihydroxyvitamin D3 through a distinct vitamin D3 receptor and a cis-acting DNA response element. In addition, recent data obtained with plasmid constructs have shown a positive interaction ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1303

    authors： Oliva A,Della Ragione F,Fratta M,Marrone G,Palumbo R,Zappia V

    更新日期：1993-03-31 00:00:00

  • Non-cyclic photoreductive carbon fixation in photosynthesis. Light and dark transients of the glycerate-3-P special pair.

    abstract：:It is demonstrated that carbon fixation in photosynthesis is regulated in two kinetically coupled pathways involving the specialized pair of non-equivalent, enzyme-bound glycerate-3-P (3-PGA) molecules obtained from ribulose 1,5-bisphosphate (RuBP) carboxylation in the light. A non-cyclic pathway is suggested (reactio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90534-7

    authors： Fong FK,Butcher KA

    更新日期：1988-01-15 00:00:00

  • Hyperpolarization-activated cation current Ih of dentate gyrus granule cells is upregulated in human and rat temporal lobe epilepsy.

    abstract：:The hyperpolarization-activated cation current I(h) is an important regulator of neuronal excitability and may contribute to the properties of the dentate gyrus granule (DGG) cells, which constitute the input site of the canonical hippocampal circuit. Here, we investigated changes in I(h) in DGG cells in human tempora...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.02.133

    authors： Surges R,Kukley M,Brewster A,Rüschenschmidt C,Schramm J,Baram TZ,Beck H,Dietrich D

    更新日期：2012-03-30 00:00:00

  • Caspase-7 mediated cleavage of proteasome subunits during apoptosis.

    abstract：:Caspase-3 and caspase-7 are structurally closely related and demonstrate overlapping substrate specificity. However, during apoptosis, they are differentially regulated and show distinct subcellular localizations, implying the presence of specific substrates. In this study, to identify caspase-7 substrates, we treated...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.183

    authors： Jang M,Park BC,Lee AY,Na KS,Kang S,Bae KH,Myung PK,Chung BC,Cho S,Lee DH,Park SG

    更新日期：2007-11-16 00:00:00

  • Homeodomain protein CDX2 regulates COX-2 expression in colorectal cancer.

    abstract：:CDX2 is an intestine-specific tumor suppressor gene encoding homeodomain-containing transcription factor, which is involved in a variety of developmental, proliferating, and differentiating processes. Moreover, the expression of CDX2 is reduced in a subset of primary colorectal cancers. In contrast, cyclooxygenase-2 (...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.020

    authors： Kim SP,Park JW,Lee SH,Lim JH,Jang BC,Lee SH,Jang IH,Freund JN,Suh SI,Mun KC,Song DK,Ha EM,Lee WJ,Kwon TK

    更新日期：2004-02-27 00:00:00

  • Receptor binding and Nb2 cell mitogenic activities of glycosylated vs. unglycosylated porcine prolactin.

    abstract：:Purified fractions of glycosylated (pGPrl) and unglycosylated (pUGPrl) porcine prolactin were prepared by affinity chromatography on Concanavalin A-Sepharose. The relative binding activities of these two forms of prolactin for receptors from porcine mammary, adrenal cortex and rabbit mammary, as well as their Nb2 cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)81265-8

    authors： Atkinson PR,Seely JE,Klemcke HG,Hughes JP

    更新日期：1988-09-30 00:00:00

  • Endothelin action on vascular smooth muscle involves inositol trisphosphate and calcium mobilization.

    abstract：:Cultured endothelial cells release a potent vasoconstrictor peptide, endothelin. Cumulative addition of synthetic endothelin to isolated rabbit aortic rings elicited a concentration-dependent increase in contractile tension which was endothelium-independent. In cultured rabbit vascular smooth muscle cells loaded with ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80180-9

    authors： Marsden PA,Danthuluri NR,Brenner BM,Ballermann BJ,Brock TA

    更新日期：1989-01-16 00:00:00

  • Impairment of social and emotional behaviors in Cadm1-knockout mice.

    abstract：:Cell adhesion molecule 1 (CADM1), a member of the immunoglobulin superfamily, mediates synaptic cell adhesion. Missense mutations in the CADM1 gene have been identified in autism spectrum disorder (ASD) patients. In the present study, we examined emotional behaviors, social behaviors and motor performances in Cadm1-kn...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.165

    authors： Takayanagi Y,Fujita E,Yu Z,Yamagata T,Momoi MY,Momoi T,Onaka T

    更新日期：2010-06-04 00:00:00

  • Octahedral complexes of anti-cancer Pt(IV)(cyclohexyldiamine) agents with 9-methylguanine.

    abstract：:The compounds [Pt(IV)(dach)(9-methylguanine)2X2]2+ (X = Cl, OH) have been prepared and structurally characterized. Their existence shows that it is possible to accommodate two purine bases (in a cis configuration) and four other ligands around a Pt(IV) atom. The geometries of these complexes have very different orient...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80749-6

    authors： Choi HK,Huang SK,Bau R

    更新日期：1988-11-15 00:00:00

  • Protostadienol synthase from Aspergillus fumigatus: functional conversion into lanosterol synthase.

    abstract：:Oxidosqualene:protostadienol cyclase (OSPC) from the fungus Aspergillus fumigatus, catalyzes the cyclization of (3S)-2,3-oxidosqualene into protosta-17(20)Z,24-dien-3beta-ol which is the precursor of the steroidal antibiotic helvolic acid. To shed light on the structure-function relationship between OSPC and oxidosqua...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.160

    authors： Kimura M,Kushiro T,Shibuya M,Ebizuka Y,Abe I

    更新日期：2010-01-01 00:00:00

  • Target disruption of ribosomal protein pNO40 accelerates aging and impairs osteogenic differentiation of mesenchymal stem cells.

    abstract：:pNO40/PS1D, a novel nucleolar protein, has been characterized as a core protein of eukaryotic 60S ribosome and at least two splicing forms of pNO40 mRNAs with alternative starting sites have been identified. Through production of knockout (ko) mice with either exon 2 (△E2), exon 4 (△E4) or △E2+E4 targeted disruption w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.003

    authors： Lin YM,Wu CC,Chang YC,Wu CH,Ho HL,Hu JW,Chang RC,Wang CT,Ouyang P

    更新日期：2016-01-22 00:00:00

  • Altered localization of amyloid precursor protein under endoplasmic reticulum stress.

    abstract：:Recent reports have shown that the endoplasmic reticulum (ER) stress is relevant to the pathogenesis of Alzheimer disease. Following the amyloid cascade hypothesis, we therefore attempted to investigate the effects of ER stress on amyloid-beta peptide (Abeta) generation. In this study, we found that ER stress altered ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.03.173

    authors： Kudo T,Okumura M,Imaizumi K,Araki W,Morihara T,Tanimukai H,Kamagata E,Tabuchi N,Kimura R,Kanayama D,Fukumori A,Tagami S,Okochi M,Kubo M,Tanii H,Tohyama M,Tabira T,Takeda M

    更新日期：2006-06-02 00:00:00

  • Oxidative DNA damage by minor metabolites of toluene may lead to carcinogenesis and reproductive dysfunction.

    abstract：:Recently, the concern that toluene might have carcinogenic and reproductive toxic potential has been raised. We investigated the ability of DNA damage by minor metabolites of toluene, methylhydroquinone, and methylbenzoquinone, using (32)P-5'-end-labeled DNA fragments obtained from the human genes. Methylhydroquinone ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1041

    authors： Murata M,Tsujikawa M,Kawanishi S

    更新日期：1999-08-02 00:00:00

  • Dicarbanonaborates--new inhibitors of mitochondrial L-glycerol-3-phosphate dehydrogenase.

    abstract：:The inhibitory effect of mercapto- and chlorodicarbanonaborates on mitochondrial, FAD-linked, L-glycerol-3-phosphate dehydrogenase activity was tested. The mercaptodicarbanonaborate was found to be a more potent inhibitor than the chloro derivative. The Ki of mercapto- and chlorodicarbanonaborates were 0.8 M and 480 M...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1724

    authors： Drahota Z,Saf P,Rauchová H,Kalous M

    更新日期：1995-05-25 00:00:00

  • Enthalpy, entropy and heat capacity changes induced by binding of calcium ions to cardiac troponin C.

    abstract：:Microcalorimetric titrations have been used to study the binding of Ca2+ to cardiac troponin C. Measurements were made both in the presence and in the absence of Mg2+, and at temperatures of 5 degrees, 15 degrees and 25 degrees C. Changes in enthalpy, entropy and heat capacity of troponin C associated with Ca binding ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91608-x

    authors： Kometani K,Yamada K

    更新日期：1983-07-18 00:00:00

  • AJAP1 affects behavioral changes and GABABR1 level in epileptic mice.

    abstract：:Adherens junction-associated protein-1 (AJAP1), also called SHREW1, was first discovered as a novel component of adherens junctions in 2004. In later studies, AJAP1 was found to suppress invasion and predict recurrence of some tumors. Apart from its function as a putative tumor suppressor, AJAP1 is still poorly unders...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.036

    authors： Zhang M,Zhou X,Jiang W,Li M,Zhou R,Zhou S

    更新日期：2020-04-16 00:00:00

  • PPARγ alleviated hepatocyte steatosis through reducing SOCS3 by inhibiting JAK2/STAT3 pathway.

    abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.110

    authors： Bi J,Sun K,Wu H,Chen X,Tang H,Mao J

    更新日期：2018-04-15 00:00:00

  • Studies on immunoproteasome in human liver. Part I: absence in fetuses, presence in normal subjects, and increased levels in chronic active hepatitis and cirrhosis.

    abstract：:Despite the central role of proteasomes in relevant physiological pathways and pathological processes, this topic is unexpectedly largely unexplored in human liver. Here we present data on the presence of proteasome and immunoproteasome in human livers from normal adults, fetuses and patients affected by major hepatic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.05.104

    authors： Vasuri F,Capizzi E,Bellavista E,Mishto M,Santoro A,Fiorentino M,Capri M,Cescon M,Grazi GL,Grigioni WF,D'Errico-Grigioni A,Franceschi C

    更新日期：2010-06-25 00:00:00

  • Development-related and cell-type specific nuclear localization of annexin XI: immunolocalization analysis in rat tissues.

    abstract：:Subcellular distribution of a novel annexin, annexin XI, was examined in rat tissues. As we have previously reported, annexin XI mainly localizes in nuclei of 3Y1, the rat embryonic fibroblast cell line; however, immunoperoxidase staining was not particularly nuclear in most of the tissues examined in adult rats. Nucl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1942

    authors： Mamiya N,Iino S,Mizutani A,Kobayashi S,Hidaka H

    更新日期：1994-07-15 00:00:00

  • Aggresome formation and pharmacogenetics: sulfotransferase 1A3 as a model system.

    abstract：:A common cause for pharmacogenetic alteration in drug response is genetic variation in encoded amino acid sequence. We have used the catecholamine and drug-metabolizing enzyme sulfotransferase (SULT)1A3 to create an artificial model system to study mechanisms-especially possible aggresome formation-by which genetic al...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.040

    authors： Wang L,Yee VC,Weinshilboum RM

    更新日期：2004-12-10 00:00:00

  • The chimeric gene linked to glucocorticoid-suppressible hyperaldosteronism encodes a fused P-450 protein possessing aldosterone synthase activity.

    abstract：:Glucocorticoid-suppressible hyperaldosteronism (GSH) is one variety of primary aldosteronism with hypertension and is inherited in an autosomal dominant mode. A recent report has indicated that GSH is caused by a gene duplication arising from unequal crossing over between the two genes, CYP11B1 and CYP11B2, encoding P...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92286-7

    authors： Miyahara K,Kawamoto T,Mitsuuchi Y,Toda K,Imura H,Gordon RD,Shizuta Y

    更新日期：1992-12-15 00:00:00

  • Effects of sequence selective drugs on the gel mobility of a bent DNA fragment.

    abstract：:The effects of various drugs on the structure of a bent DNA fragment have been investigated by studying DNA mobility in polyacrylamide gels. This DNA fragment has an anomalously slow rate of migration on account of its phased runs of adenines. Nogalamycin and echinomycin increase the gel mobility of kinetoplast DNA su...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90792-l

    authors： Cons BM,Fox KR

    更新日期：1990-09-28 00:00:00