cDNA cloning and characterization of vinculin mRNA.

abstract：:Little is known about an oncogenic signal transducer β-1,4-galactosyltransferase-V (β-1,4-GalT-V), in human colorectal cancer. Using quantitative RT-PCR, immunohistochemical staining and ELISA assays, we determined that β-1,4-GalT-V gene/protein expression is specifically increased in human colorectal cancer (CRC) tum...

journal_title：Biochemical and biophysical research communications

authors： Chatterjee SB,Hou J,Bandaru VVR,Pezhouh MK,Syed Rifat Mannan AA,Sharma R

更新日期：2019-01-08 00:00:00

abstract：:Northern hybridization studies showed that the level of ovalbumin mRNA decreases in the oviduct of the Japanese quail after adulthood. In order to find out if this is due to changes in the conformation of the chromatin containing the promoter region of the gene, nuclei of the oviduct of young, adult and old birds were...

journal_title：Biochemical and biophysical research communications

authors： Upadhyay R,Gupta S,Kanungo MS

更新日期：1996-09-13 00:00:00

abstract：:Adiponectin is an adipocyte-derived hormone and known to form several species of multimer, however, the precise components of each multimer have not been fully determined. We purified each multimer adiponectin selectively from human plasma and characterized them by affinity columns using anti-adiponectin, gelatin, or ...

journal_title：Biochemical and biophysical research communications

authors： Hada Y,Yamauchi T,Waki H,Tsuchida A,Hara K,Yago H,Miyazaki O,Ebinuma H,Kadowaki T

更新日期：2007-05-04 00:00:00

abstract：:Hematopoiesis is a complex process tightly controlled by sets of transcription factors in a context-dependent and stage-specific manner. Smad2/3 transcription factor plays a central role in differentiation and survival of erythroid cells. Here we report that follistatin-like 1 (FSTL1) treatment impairs hemin-induced e...

journal_title：Biochemical and biophysical research communications

authors： Wu J,Dong Y,Teng X,Cheng M,Shen Z,Chen W

更新日期：2015-10-30 00:00:00

abstract：:Posttranslational histone modifications play an important role in modulating gene expression and chromatin structure. Here we report the identification of histone H3K79 dimethylation in the simple eukaryote Dictyostelium discoideum. We have deleted the D. discoideum Dot1/KMT4 homologue and demonstrate that it is the s...

journal_title：Biochemical and biophysical research communications

authors： Müller-Taubenberger A,Bönisch C,Fürbringer M,Wittek F,Hake SB

更新日期：2011-01-28 00:00:00

abstract：:Folate deficiency produces a marked decrease in the ratio of S-adenosylmethionine to S-adenosylhomocysteine. This ratio regulates the activity of many cellular methylation reactions including that of DNA. We used bacterial CpG methylase to evaluate the effect of folate deficiency on methylation of DNA from livers of w...

journal_title：Biochemical and biophysical research communications

authors： Balaghi M,Wagner C

更新日期：1993-06-30 00:00:00

abstract：:The disruption of the intracellular Ca(2+) homeostasis of Leishmania mexicana represents a major target for the action of drugs, such as amiodarone and miltefosine. However, little is known about the mechanism of Ca(2+) entry to these cells. Here we show the presence of a Ca(2+) channel in the plasma membrane of these...

journal_title：Biochemical and biophysical research communications

authors： Benaim G,García-Marchán Y,Reyes C,Uzcanga G,Figarella K

更新日期：2013-01-18 00:00:00

abstract：:A novel aptameric enzyme subunit (AES) which can detect target DNAs has been developed. AES is an enzyme-inhibiting aptamer bearing a target-molecule binding site which can allosterically control enzymatic activity. The thrombin-inhibiting aptamer bearing a G-quartet structure was chosen as the enzyme-inhibiting aptam...

journal_title：Biochemical and biophysical research communications

authors： Yoshida W,Sode K,Ikebukuro K

更新日期：2006-09-15 00:00:00

abstract：:Nitric oxide (NO) production is increased in the intestine and may contribute to intestinal injury in sepsis. However, the tissue expression of inducible NO synthase (iNOS) mRNA throughout the digestive tract and its relation with the mucosal damage after endotoxin challenge remain unknown. We therefore measured tissu...

journal_title：Biochemical and biophysical research communications

authors： Chen K,Inoue M,Okada A

更新日期：1996-07-25 00:00:00

abstract：:Receptor binding properties of the hemoglobin-derived nonapeptide VV-hemorphin 7 (Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg-Phe-OH) were studied using both the unlabelled form and tritium-labelled derivative of the peptide. In binding studies using selective opioid radioligands, VV-hemorphin 7 exhibited a rank order of potency ...

journal_title：Biochemical and biophysical research communications

authors： Szikra J,Benyhe S,Orosz G,Darula Z,Piot JM,Fruitier I,Monory K,Hanoune J,Borsodi A

更新日期：2001-03-02 00:00:00

abstract：:We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of ex...

journal_title：Biochemical and biophysical research communications

authors： Chavez JA,Gridley S,Sano H,Lane WS,Lienhard GE

更新日期：2006-04-21 00:00:00

abstract：:MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB...

journal_title：Biochemical and biophysical research communications

authors： Tian W,Wang G,Liu Y,Huang Z,Zhang C,Ning K,Yu C,Shen Y,Wang M,Li Y,Wang Y,Zhang B,Zhao Y

更新日期：2017-03-25 00:00:00

abstract：:Although N-acetyl-L-aspartate (NAA) has been shown to be important to myelin synthesis and osmotic regulation, the biological rationale for the high levels of NAA found in the brain remains unknown. Here, a human astroglial cell line (STTG) was treated with NAA and stimulated with ionomycin, ionomycin/PMA, or IL-1beta...

journal_title：Biochemical and biophysical research communications

authors： Rael LT,Thomas GW,Bar-Or R,Craun ML,Bar-Or D

更新日期：2004-07-02 00:00:00

abstract：:Airway viral infection disturbs the health-related quality of life. B7-H1 (also known as PD-L1) is a coinhibitory molecule associated with the escape of viruses from the mucosal immunity, leading to persistent infection. Most respiratory viruses generate double-stranded (ds) RNA during replication. The stimulation of ...

journal_title：Biochemical and biophysical research communications

authors： Kan-o K,Matsumoto K,Asai-Tajiri Y,Fukuyama S,Hamano S,Seki N,Nakanishi Y,Inoue H

更新日期：2013-05-31 00:00:00

abstract：:In humans, mutations in the gene encoding cereblon (CRBN) are associated with mental retardation. Although CRBN has been investigated in several cellular contexts, its function remains unclear. Here, we demonstrate that CRBN plays a role in regulating the ubiquitin-proteasome system (UPS). Heterologous expression of C...

journal_title：Biochemical and biophysical research communications

authors： Lee KM,Lee J,Park CS

更新日期：2012-10-26 00:00:00

abstract：:The initiating factors in primary, idiopathic osteoarthritis are unknown, the characteristic bone and cartilage changes being late features of the disease. We have proposed that biochemical cruciate ligament alteration may be important in early osteoarthritis by mediating loading consequences on the bone and cartilage...

journal_title：Biochemical and biophysical research communications

authors： Anderson-MacKenzie JM,Billingham ME,Bailey AJ

更新日期：1999-05-19 00:00:00

abstract：:Although D-glucosamine has been reported as an inhibitor of tumor growth both in vivo and in vitro, the mechanism for the anticancer effect of D-glucosamine is still unclear. Since there are several reports suggesting D-glucosamine inhibits protein synthesis, we examined whether D-glucosamine affects p70S6K activity, ...

journal_title：Biochemical and biophysical research communications

authors： Oh HJ,Lee JS,Song DK,Shin DH,Jang BC,Suh SI,Park JW,Suh MH,Baek WK

更新日期：2007-09-07 00:00:00

abstract：:A novel neuropeptide which stimulates adenylate cyclase in rat anterior pituitary cell cultures was isolated from ovine hypothalamic tissues. Its amino acid sequence was revealed as: His-Ser-Asp-Gly-Ile-Phe-Thr-Asp-Ser-Tyr-Ser-Arg-Tyr-Arg-Lys-Gln- Met-Ala- Val-Lys-Lys-Tyr-Leu-Ala-Ala-Val-Leu-Gly-Lys-Arg-Tyr-Lys-Gln-Ar...

journal_title：Biochemical and biophysical research communications

authors： Miyata A,Arimura A,Dahl RR,Minamino N,Uehara A,Jiang L,Culler MD,Coy DH

更新日期：1989-10-16 00:00:00

abstract：:We have examined the role of K+ channels in mediating vasorelaxation produced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat aorta precontracted with phenylephrine (1 microM). Salmonella typhosa LPS (0.1 microgram/ml) caused significant relaxation of tension which peaked at approximately 4h...

journal_title：Biochemical and biophysical research communications

authors： Hall S,Turcato S,Clapp L

更新日期：1996-07-05 00:00:00

abstract：:A polyclonal antiserum raised against purified alpha-agglutinin was made specific for alpha-agglutinin after adsorption with a cells. The adsorbed antiserum identified alpha-agglutinin peptides on Western blots and bound to cell surface alpha-agglutinin, inhibiting the binding of alpha cells to a cells. Using the anti...

journal_title：Biochemical and biophysical research communications

authors： Wojciechowicz D,Lipke PN

更新日期：1989-05-30 00:00:00

abstract：:Poly (ADP-ribose) polymerase 1 (PARP1) is an ADP- ribosylation enzyme and plays important roles in a variety of cellular processes, including DNA damage response and tumor development. However, the post-transcriptional regulation of PARP1 remains largely unknown. In this study, we identified that the mRNA of PARP1 is ...

journal_title：Biochemical and biophysical research communications

authors： Song D,Huang H,Wang J,Zhao Y,Hu X,He F,Yu L,Wu J

更新日期：2017-06-17 00:00:00

abstract：:Mutations of the GJB2 gene, encoding connexin 26, are the most common cause of hereditary congenital hearing loss in many countries and account for up to 50% of cases of autosomal-recessive non-syndromic deafness. By contrast, only a few GJB2 mutations have been reported to cause an autosomal-dominant form of non-synd...

journal_title：Biochemical and biophysical research communications

authors： Melchionda S,Bicego M,Marciano E,Franzè A,Morgutti M,Bortone G,Zelante L,Carella M,D'Andrea P

更新日期：2005-11-25 00:00:00

abstract：:Several studies have shown that transplantation of embryonic stem cells into diabetic animals either improved or normalized blood glucose levels. In this study, we examined the dose-dependent effect of early (prediabetic stage) intravenous administration of human umbilical cord blood (HUCB) mononuclear cells on blood ...

journal_title：Biochemical and biophysical research communications

authors： Ende N,Chen R,Reddi AS

更新日期：2004-12-17 00:00:00

abstract：:Expression of TNF-alpha gene in the retinal pigment epithelial (RPE) cells was studied by using polymerase chain reaction (PCR). PCR for human TNF-alpha gene using complementary DNA (cDNA) of control RNA prepared from non-stimulated RPE cells failed to show expression of TNF-alpha gene. However, PCR by using cDNA prep...

journal_title：Biochemical and biophysical research communications

authors： Tanihara H,Yoshida M,Yoshimura N

更新日期：1992-09-16 00:00:00

abstract：:Vaspin, an adipocytokine recently identified in a rat model of type 2 diabetes, has been suggested to have an insulin-sensitizing effect. However, the exact mechanism underlying this action has not been fully elucidated. Furthermore, the specific function of vaspin is largely unknown, especially in vascular cells. We ...

journal_title：Biochemical and biophysical research communications

authors： Jung CH,Lee WJ,Hwang JY,Seol SM,Kim YM,Lee YL,Park JY

更新日期：2011-09-23 00:00:00

abstract：:Lafora disease, a progressive myoclonus epilepsy, is an autosomal recessive disease caused in approximately 80% of cases by mutation of the EPM2A gene, which encodes a dual specificity protein phosphatase called laforin. In addition to its phosphatase domain, laforin contains an N-terminal carbohydrate-binding domain ...

journal_title：Biochemical and biophysical research communications

authors： Wang W,Roach PJ

更新日期：2004-12-17 00:00:00

abstract：:Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced prote...

journal_title：Biochemical and biophysical research communications

authors： Pride H,Yu Z,Sunchu B,Mochnick J,Coles A,Zhang Y,Buffenstein R,Hornsby PJ,Austad SN,Pérez VI

更新日期：2015-02-20 00:00:00

abstract：:In the mouse fibroblasts BP-A31 as well as in the human epidermoid carcinoma cells KB-3-1, both cyclin D1 mRNA and protein contents decreased rapidly during incubation with sodium butyrate. The decrease of cyclin D1 mRNA was not prevented by cycloheximide indicating that protein synthesis is not required for the inhib...

journal_title：Biochemical and biophysical research communications

authors： Lallemand F,Courilleau D,Sabbah M,Redeuilh G,Mester J

更新日期：1996-12-04 00:00:00

abstract：:To simultaneously assess the effects of exonic mutations on RNA splicing and protein functions, we report here an intron-inclusive cDNA (Intinc) expression system. As a test model, twenty-four mutations in exon 9 of the phenylalanine hydroxylase (PAH) gene were examined in an Intinc expression plasmid composed of the ...

journal_title：Biochemical and biophysical research communications

authors： Ho PY,Huang MZ,Fwu VT,Lin SC,Hsiao KJ,Su TS

更新日期：2008-09-05 00:00:00

abstract：:The internucleosomal cleavage of genomic DNA is a biochemical hallmark of apoptosis. DNase gamma, a Mg2+/Ca2+-dependent endonuclease, has been suggested to be one of the apoptotic endonucleases, but its biochemical characteristic has not been fully elucidated. Here, using recombinant DNase gamma, we showed that DNase ...

journal_title：Biochemical and biophysical research communications

authors： Mizuta R,Mizuta M,Araki S,Shiokawa D,Tanuma S,Kitamura D

更新日期：2006-06-30 00:00:00