Lactosylceramide synthase β-1,4-GalT-V: A novel target for the diagnosis and therapy of human colorectal cancer.


:Little is known about an oncogenic signal transducer β-1,4-galactosyltransferase-V (β-1,4-GalT-V), in human colorectal cancer. Using quantitative RT-PCR, immunohistochemical staining and ELISA assays, we determined that β-1,4-GalT-V gene/protein expression is specifically increased in human colorectal cancer (CRC) tumors, compared to visibly normal tissue. Furthermore, we observed a marked increase in its enzymatic activity, and its product lactosylceramide. Moreover, we found increased dihydrosphingolipid metabolites, in particular dihydrosphingomyelin in cancer tissue compared to normal. Further, inhibition of glycosphingolipid synthesis by the synthetic ceramide analog, D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), concurrently inhibited colorectal cancer cell (HCT-116) proliferation, as well as β-1,4-GalT-V mass and several glycosphingolipid levels. We conclude that β-1,4-GalT-V may serve as a diagnostic and therapeutic biomarker for the progression of human colorectal cancer, and consequently, inhibition of GSL synthesis may be a novel approach for the treatment of this life-threatening disease.


Chatterjee SB,Hou J,Bandaru VVR,Pezhouh MK,Syed Rifat Mannan AA,Sharma R




Has Abstract


2019-01-08 00:00:00














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    abstract::Glycosylation of endothelin (ET) receptors was found to occur in rat cerebellar and atrial membranes. Specifically, we investigated whether the ETA and ETB receptor subtypes differed in their sensitivity to deglycosylation treatment and whether the two affinity states (nanomolar and picomolar) observed in each recepto...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shraga-Levine Z,Sokolovsky M

    更新日期:1998-05-19 00:00:00

  • Preliminary assessment of the C13-side chain 2'-hydroxylase involved in taxol biosynthesis.

    abstract::The biosynthesis of the anticancer drug Taxol in yew (Taxus) species is thought to involve the preliminary formation of the advanced taxane diterpenoid intermediate baccatin III upon which the functionally important N-benzoyl phenylisoserinoyl side chain is subsequently assembled at the C13-O-position. In vivo feeding...

    journal_title:Biochemical and biophysical research communications

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    authors: Long RM,Croteau R

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  • GLP-1 contributes to increases in PGC-1α expression by downregulating miR-23a to reduce apoptosis.

    abstract::GLP-1 can help to overcome problems of liver cells metabolism, not only pancreatic cell. But the explicit mechanism of this effect remains unclear. In recent years, microRNAs have received the attention of researchers and some microRNAs have important implications for diabetes. The mitochondrial protective gene PGC-1α...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang C,Li Q,Wang W,Guo L,Guo C,Sun Y,Zhang J

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  • Cancer cell binding to E-selectin transfected human endothelia.

    abstract::Human endothelial cells were transiently transfected with E-Selectin which enabled us to study tumor cell/endothelial interactions following engagement of E-Selectin without the added complications of metabolic stimulation, morphological changes, and/or up regulation of other adhesion molecules due to cytokine inducti...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

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  • hnRNP A2 and hnRNP L bind the 3'UTR of glucose transporter 1 mRNA and exist as a complex in vivo.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • Glycyrrhetinic acid alleviates acute lung injury by PI3K/AKT suppressing macrophagic Nlrp3 inflammasome activation.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang K,Zhang Y,Cao Y,Shi Z,Lin Y,Chen Y,Zhao H,Liu X

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    journal_title:Biochemical and biophysical research communications

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  • Leukotriene signaling via ALOX5 and cysteinyl leukotriene receptor 1 is dispensable for in vitro growth of CD34+CD38- stem and progenitor cells in chronic myeloid leukemia.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 临床试验,杂志文章


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    journal_title:Biochemical and biophysical research communications

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • (2R,5R)-6-heptyne-2,5-diamine, an extremely potent inhibitor of mammalian ornithine decarboxylase.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • Dephosphorylation of Girdin by PP2A inhibits breast cancer metastasis.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • Tissue-specific splice variants of HARE/Stabilin-2 are expressed in bone marrow, lymph node, and spleen.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • Esco2 is a novel corepressor that associates with various chromatin modifying enzymes.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • Ubiquitin-conjugating enzyme Cdc34 mediates cadmium resistance in budding yeast through ubiquitination of the transcription factor Met4.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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  • miR-135a inhibition protects A549 cells from LPS-induced apoptosis by targeting Bcl-2.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kaur R,Tiwari SK

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Salimath BP,Satyanarayana MN

    更新日期:1987-10-14 00:00:00

  • The binding of monomeric amyloid β peptide to serum albumin is affected by major plasma unsaturated fatty acids.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Litus EA,Kazakov AS,Sokolov AS,Nemashkalova EL,Galushko EI,Dzhus UF,Marchenkov VV,Galzitskaya OV,Permyakov EA,Permyakov SE

    更新日期:2019-03-05 00:00:00

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Owusu S,Cunliffe WJ,Wood EJ

    更新日期:1989-06-30 00:00:00

  • Elevated glucose concentrations inhibit DNA synthesis and expression of c-myc in cultured hepatocytes.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    更新日期:1994-08-15 00:00:00

  • Involvement of adenosine kinase in the phosphorylation of formycin B in CHO cells.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mehta KD,Gupta RS

    更新日期:1985-07-31 00:00:00