Homogeneous DNA sensing using enzyme-inhibiting DNA aptamers.

abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

journal_title：Biochemical and biophysical research communications

authors： Tian H,Hou X,Liu X

更新日期：2013-02-08 00:00:00

abstract：:Mutations in the WNK1 and WNK4 genes result in an inherited hypertensive disease, pseudohypoaldosteronism type II (PHAII). Recently, the KLHL3 and Cullin3 genes were also identified as responsible genes for PHAII. Although we have reported that WNK4 is a substrate for the KLHL3-Cullin3 E3 ligase complex, it is not cle...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Mori T,Wakabayashi M,Mori Y,Susa K,Zeniya M,Sohara E,Rai T,Sasaki S,Uchida S

更新日期：2013-08-02 00:00:00

abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

journal_title：Biochemical and biophysical research communications

authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

更新日期：2017-04-22 00:00:00

abstract：:Plants are often exposed not only to short-term (S-) heat stress but also to diurnal long-term (L-) heat stress over several consecutive days; nevertheless, most previous studies of heat tolerance have used S-heat stress, such as 42 °C for 30-60 min, for evaluation. Yet the mechanisms underlying L-heat tolerance remai...

journal_title：Biochemical and biophysical research communications

authors： Ono M,Isono K,Sakata Y,Taji T

更新日期：2021-01-01 00:00:00

abstract：:Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as ...

journal_title：Biochemical and biophysical research communications

authors： Kumar RK,Smith GJ,Truscott JY,Hristoforidis CP,Lykke AW

更新日期：1986-09-30 00:00:00

abstract：:Dilated cardiomyopathy (DCM) is a major cause of heart failure in younger individuals. Its prognosis is poor with 40-50% of patients dying within 2 years after diagnosis. Although the etiology of DCM is poorly understood, there is increasing evidence that DCM may represent an autoimmune disease in a significant subset...

journal_title：Biochemical and biophysical research communications

authors： Nickenig G,Wolff M,Stäblein A,Pfister H,Böhm M

更新日期：1999-12-20 00:00:00

abstract：:AMP, an allosteric inhibitor at neutral pH, activates snakes muscle fructose 1,6-bisphosphatase at pH 9.2. The activation is virtually unique for the snake muscle enzyme: activation was not observed for the enzymes from either human and rabbit liver or porcine kidney. The activation is Mg(2+)-dependent but was not obs...

journal_title：Biochemical and biophysical research communications

authors： Zhao FK,Xu SQ,Xu GJ

更新日期：1998-03-27 00:00:00

abstract：:NADH-rubredoxin oxidoreductase (NROR), a flavoprotein from the obligately anaerobe Clostridium acetobutylicum is encoded by an ORF (nror) of 1140 nucleotides. Whereas primary structure analysis reveals that NROR has amino acid sequence patterns homologous with those involved in FAD and NAD-binding, the enzyme is dista...

journal_title：Biochemical and biophysical research communications

authors： Guedon E,Petitdemange H

更新日期：2001-07-13 00:00:00

abstract：:RNA interference (RNAi) mediated inhibition of virus-specific genes has emerged as a potential therapeutic strategy against virus induced diseases. Human hepatitis B virus (HBV) surface antigen (HBsAg) has proven to be a significant risk factor in HBV induced liver diseases, and an increasing number of mutations in HB...

journal_title：Biochemical and biophysical research communications

authors： Cheng TL,Chang WW,Su IJ,Lai MD,Huang W,Lei HY,Chang WT

更新日期：2005-10-28 00:00:00

abstract：:Prion diseases are fatal neurodegenerative diseases associated with structural conversion of α-helical prion protein (PrP) into its β-sheet rich isoform (PrPSc). Previous genetic analyses have indicated that several amino acid residues involved in the hydrophobic core of PrP (such as V180, F198, and V210) play a criti...

journal_title：Biochemical and biophysical research communications

authors： Kakuda K,Yamaguchi KI,Kuwata K,Honda R

更新日期：2018-11-17 00:00:00

abstract：:The filamentous fungus Aspergillus ochraceus TS produces an inducible microsomal cytochrome P-450 linked monooxygenase which is capable of hydroxylating benzo(a)pyrene in presence of O2 and NADPH. The addition of Benzo(a)pyrene, 3-Methyl cholanthrene, beta-Naphthoflavone and other aryl hydrocarbons during the inductio...

journal_title：Biochemical and biophysical research communications

authors： Dutta D,Ghosh DK,Mishra AK,Samanta TB

更新日期：1983-09-15 00:00:00

abstract：:This study provides evidence for the presence of large conductance Ca(2+)-dependent K-channels in guinea pig and human urinary bladder smooth muscle. A23187, a Ca(2+)-ionophore, increased charybdotoxin and iberiatoxin sensitive 42K efflux in human urinary bladder smooth muscle cells, suggesting that large conductance ...

journal_title：Biochemical and biophysical research communications

authors： Trivedi S,Potter-Lee L,Li JH,Yasay GD,Russell K,Ohnmacht CJ,Empfield JR,Trainor DA,Kau ST

更新日期：1995-08-15 00:00:00

abstract：:Granulocytin, a C-type lectin from Sarcophaga peregrina (flesh fly), stimulated glucose consumption and cytokine production by the mouse macrophage-like cell line J774.1. When J774.1 cells were pretreated with tunicamycin, their granulocytin-dependent TNF-alpha production was greatly reduced. These results suggest tha...

journal_title：Biochemical and biophysical research communications

authors： Fujita Y,Homma KJ,Natori S

更新日期：2000-09-07 00:00:00

abstract：:REV-ERBα is a nuclear heme receptor, transcriptional repressor and critical component of the molecular clock that drives daily rhythms of metabolism. Evidence reveals that REV-ERBα also plays an important regulatory role in clock-dependent lung physiology and inflammatory responses. We hypothesize that cigarette smoke...

journal_title：Biochemical and biophysical research communications

authors： Sundar IK,Rashid K,Sellix MT,Rahman I

更新日期：2017-12-02 00:00:00

abstract：:Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is the only known intracellular antioxidant enzyme that can directly reduce lipid hydroperoxide in membrane. Mitochondrial and non-mitochondrial PHGPx and sperm nuclei GPx are transcribed from one gene by alternative transcription using different first exons Ia...

journal_title：Biochemical and biophysical research communications

authors： Imai H,Hirao F,Sakamoto T,Sekine K,Mizukura Y,Saito M,Kitamoto T,Hayasaka M,Hanaoka K,Nakagawa Y

更新日期：2003-05-30 00:00:00

abstract：:Gonadotropin releasing hormone (GnRH), preincubated with cultured rat pituitary cells, induced down regulation of GnRH receptors in a time- and dose-dependent manner. The specific binding was inhibited by 50% after 30 min and maximal inhibition (70%) was obtained after 75 min preincubation with 1 microM GnRH. Preincub...

journal_title：Biochemical and biophysical research communications

authors： Hazum E,Schvartz I

更新日期：1984-12-14 00:00:00

abstract：:Saccharomyces cerevisiae RecQ helicase (Sgs1) combines with DNA topoisomerase III (Top3) and RecQ-mediated genome instability 1 (Rmi1) to form an evolutionarily conserved complex that is required for processing homologous recombination intermediates and restarting collapsed replication forks. It was previously reporte...

journal_title：Biochemical and biophysical research communications

authors： Lai MS,Seki M,Tada S,Enomoto T

更新日期：2012-10-26 00:00:00

abstract：:Cultivated rice (Oryza sativa) possesses various labdane-related diterpene synthase genes, homologs of ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) that are responsible for the biosynthesis of phytohormone gibberellins. The CPS homologs and KS like (KSL) homologs successively converted geranylg...

journal_title：Biochemical and biophysical research communications

authors： Toyomasu T,Miyamoto K,Shenton MR,Sakai A,Sugawara C,Horie K,Kawaide H,Hasegawa M,Chuba M,Mitsuhashi W,Yamane H,Kurata N,Okada K

更新日期：2016-11-18 00:00:00

abstract：:The brain-type glucose transporter (bGT) is assumed to be distributed among neurons and glial cells, but to be particularly enriched in brain microvascular endothelium, which makes up the blood-brain barrier (BBB) in vivo. However, in the course of the present studies it was found that the bGT is specifically expresse...

journal_title：Biochemical and biophysical research communications

authors： Boado RJ,Pardridge WM

更新日期：1990-01-15 00:00:00

abstract：:It is considered that the essence of acute lung injury (ALI) is an excessive and uncontrolled inflammatory response in lung, of which mainly is attributed to the release of inflammatory mediators. Recent studies demonstrated that irisin, which is a metabolism associated factor after physical exercise could suppression...

journal_title：Biochemical and biophysical research communications

authors： Shao L,Meng D,Yang F,Song H,Tang D

更新日期：2017-05-27 00:00:00

abstract：:We linked a 3.3 kb fragment containing the human gamma-globin gene together with a 5.5 kb fragment containing the human beta-globin gene. This construct was introduced into the germ line of mice. Analysis of the resulting transgenic mice showed that expression of the human gamma- and beta-globin genes were regulated a...

journal_title：Biochemical and biophysical research communications

authors： Katsube T,Tojo H,Sakaguchi T,Fukumaki Y

更新日期：1993-07-15 00:00:00

abstract：:Pigment epithelium-derived factor (PEDF) encoded by serpinf1 is a potent antiangiogenic factor found in a wide variety of fetal and adult tissues. Several reports have shown that lack of PEDF leads to osteogenesis imperfecta (OI) type VI whose hallmark is a defect in mineralization that leads to excessive osteoid buil...

journal_title：Biochemical and biophysical research communications

authors： Li F,Armstrong GB,Tombran-Tink J,Niyibizi C

更新日期：2016-09-23 00:00:00

abstract：:We have cloned the cDNA encoding human PICK1 (protein interacting with C kinase 1), a PDZ domain-containing protein of 415 amino acids, and also identified the Drosophila homologue by search of the databank. Northern blot analysis shows a single mRNA of about 2.0 kb ubiquitously expressed in human tissues. Although PI...

journal_title：Biochemical and biophysical research communications

authors： Takeya R,Takeshige K,Sumimoto H

更新日期：2000-01-07 00:00:00

abstract：:We recently developed the cellular model MVLN-15 in which estrogenic action can be detected by bioluminescence. Using this cellular model, we characterized the inhibitory effect of retinoic acid on the estrogen-dependent induction of luciferase transcription. We present evidence that i) the inhibitory effect of retino...

journal_title：Biochemical and biophysical research communications

authors： Demirpence E,Pons M,Balaguer P,Gagne D

更新日期：1992-02-28 00:00:00

abstract：:A major protein AA amyloid protein was purified and characterised from a Papua New Guinnean individual. This AA protein differed from all previously characterised SAA variants by the combination of Ala52, Val57, Asn60, Phe68, Phe69 and Gly72. Since the prevalence of AA-amyloidosis is unusually high in Papua New Guinea...

journal_title：Biochemical and biophysical research communications

authors： Westermark P,Sletten K,Westermark GT,Raynes J,McAdam KP

更新日期：1996-06-14 00:00:00

abstract：:Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators...

journal_title：Biochemical and biophysical research communications

authors： Olofsson KE,Andersson L,Nilsson J,Björkbacka H

更新日期：2008-05-30 00:00:00

abstract：:Inflammatory bowel disease (IBD) comprises two major subtypes, ulcerative colitis (UC) and Crohn's disease, which are multifactorial diseases that may develop due to genetic susceptibility, dysbiosis, or environmental factors. Environmental triggers of IBD include food-borne factors, and a previous nationwide survey i...

journal_title：Biochemical and biophysical research communications

authors： Tsuchiya M,Ito G,Hama M,Nagata S,Kawamoto A,Suzuki K,Shimizu H,Anzai S,Takahashi J,Kuno R,Takeoka S,Hiraguri Y,Sugihara HY,Mizutani T,Yui S,Oshima S,Tsuchiya K,Watanabe M,Okamoto R

更新日期：2021-01-20 00:00:00

abstract：:The Notch3 N-terminal sequence is conserved across several mammalian species but diverges from the three other Notch proteins. We determined the significance of the N-terminal sequence using deletion mutants. The first 39 amino acids are required for Notch3 receptor expression, processing, and functional activity. In ...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Jia L,Lee SJ,Wang MM

更新日期：2007-03-30 00:00:00

abstract：:Hepatitis C virus (HCV) infects its target cells in the form of cell-free viruses and through cell-cell contact. Here we report that HCV is associated with exosomes. Using highly purified exosomes and transmission electron microscopic imaging, we demonstrated that HCV occurred in both exosome-free and exosome-associat...

journal_title：Biochemical and biophysical research communications

authors： Liu Z,Zhang X,Yu Q,He JJ

更新日期：2014-12-12 00:00:00

abstract：:MicroRNAs (miRNAs) are small RNA molecules, which act as post-transcriptional regulators of a gene expression, with important functions within the cell physiology. Whilst many authors have focused on the study of miRNA expression in physiological and pathological processes, various technical variables related to miRNA...

journal_title：Biochemical and biophysical research communications

authors： Mraz M,Malinova K,Mayer J,Pospisilova S

更新日期：2009-12-04 00:00:00