当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > MicroRNA isolation and stability in stored RNA samples.

MicroRNA isolation and stability in stored RNA samples.

Abstract：

:MicroRNAs (miRNAs) are small RNA molecules, which act as post-transcriptional regulators of a gene expression, with important functions within the cell physiology. Whilst many authors have focused on the study of miRNA expression in physiological and pathological processes, various technical variables related to miRNA isolation have simultaneously emerged and the stability of the stored miRNA samples has been questioned. A robust method for RNA isolation is essential for reproducible results and miRNAs instability in the stored samples would make for an alarming situation for most expression studies. Here these issues are discussed and we investigate the stability of miRNAs isolated from clinical samples of B lymphocytes (chronic lymphocytic leukemia) by the most commonly utilized method based on a Trizol/TRI-Reagent solution (RNAs stored at -80 degrees C). To assess the stability of miRNAs, a Real Time-PCR analysis was performed for a panel of 29 miRNAs from a freshly isolated RNA sample and after 14 days storage at -80 degrees C. Furthermore, a Real Time-PCR analysis was repeatedly performed for a stored RNA sample over a period of approximately 10 months. We observed high stability of isolated miRNAs and respective cDNAs. The reproducibility and efficiency of the Trizol/TRI-Reagent isolation method was also tested and compared to the mirVana Isolation kit (Ambion) and RNeasy kit (Qiagen). In conclusion, Trizol/TRI-Reagent based isolation is a robust reproducible method, and obtained miRNA samples do not show any tendency to degradation when properly stored and handled.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mraz M,Malinova K,Mayer J,Pospisilova S

doi

10.1016/j.bbrc.2009.09.061

subject

Has Abstract

pub_date

2009-12-04 00:00:00

pages

1-4

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01863-4

journal_volume

390

pub_type

杂志文章,评审

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Evidence for constitutive dimerization of niacin receptor subtypes.

    abstract：:The recently deorphanized niacin receptor subtypes NIACR1 (GPR109A) and NIACR2 (GPR109B) play an essential role in the regulation of metabolic processes and immune reactions. Both receptors belong to the G-protein-coupled receptor (GPCR) family, whose members have traditionally been treated as monomeric entities, but ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.011

    authors： Mandrika I,Petrovska R,Klovins J

    更新日期：2010-04-30 00:00:00

  • In vivo formation of hepoxilin A3 in the rat.

    abstract：:Bolus intravenous injection of arachidonic acid (10 mg/kg) in the rat led to the appearance of hepoxilin A3 in the circulation. The product was assayed as the Me t-BDMSi derivative of its stable trihydroxy product trioxilin A3, by capillary gas chromatography-electron impact mass spectrometry using the stable deuteriu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80152-3

    authors： Pace-Asciak CR,Lee SP,Martin JM

    更新日期：1987-09-30 00:00:00

  • MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.

    abstract：BACKGROUND AND AIM:Aberrant expression of miR-107 is involved in the development of several human cancers. However, the role of miR-107 in hepatocellular carcinoma (HCC) is not well documented. In the present study, we aim to explore the function of miR-107 in hepatocarcinogenesis. METHODS:Bioinformatics analysis was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.070

    authors： Wang Y,Chen F,Zhao M,Yang Z,Zhang S,Ye L,Gao H,Zhang X

    更新日期：2016-11-18 00:00:00

  • Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes.

    abstract：:The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.03.057

    authors： Dossymbekova R,Bgatova N,Tungushbayeva Z,Sharipov K,Taneyeva G,Kydyrbaeva A,Solovieva A

    更新日期：2020-07-23 00:00:00

  • Dissect Kif5b in nuclear positioning during myogenesis: the light chain binding domain and the autoinhibitory peptide are both indispensable.

    abstract：:The microtubule motor kinesin-1 is responsible for the nuclear positioning during myogenesis. Here we show that the coiled-coil stalk/tail domain containing the kinesin light chain (KLC) binding sites targets to the perinuclear region like endogenous Kif5b, while the globular tail domain cannot. To investigate which f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.02.006

    authors： Wang Z,Xue W,Li X,Lin R,Cui J,Huang JD

    更新日期：2013-03-08 00:00:00

  • Binding of deoxyadenylate and deoxycytidylate antibodies to double-stranded DNA.

    abstract：:Antibodies raised against deoxyadenylate and deoxycytidylate were found to react with double stranded DNA as assessed by highly sensitive avidin-biotin microELISA. The binding was specific as it was completely inhibited by the homologous hapten. The antibodies did not react with tRNA and rRNA. These antibodies were al...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90658-4

    authors： Vaishnav YN,Antony A

    更新日期：1988-07-15 00:00:00

  • cDNA cloning of a murine homologue of Drosophila single-minded, its mRNA expression in mouse development, and chromosome localization.

    abstract：:A combination of the RT-PCR method and subsequent screening of the cDNA library of mouse skeletal muscle with the cDNA isolated by RT-PCR used as a probe led to isolation of cDNAs encoding a polypeptide (mSim) with bHLH and PAS domains which show high similarity to the corresponding regions of Drosophila Sim, a master...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0104

    authors： Ema M,Suzuki M,Morita M,Hirose K,Sogawa K,Matsuda Y,Gotoh O,Saijoh Y,Fujii H,Hamada H,Fujii-Kuriyama Y

    更新日期：1996-01-17 00:00:00

  • Molecular cloning of anti-SS-A/Ro 60-kDa peptide Fab fragments from infiltrating salivary gland lymphocytes of a patient with Sjögren's syndrome.

    abstract：:Anti-SS-A/Ro antibodies are commonly found in systemic autoimmune diseases such as Sjögren's syndrome and systemic lupus erythematosus, and some of these antibodies appear to be responsible for certain pathological lesions including congenital heart block in neonatal lupus. In this study, we generated three human anti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6233

    authors： Suzuki H,Takemura H,Suzuki M,Sekine Y,Kashiwagi H

    更新日期：1997-03-06 00:00:00

  • Homology-modeled ligand-binding domains of medaka estrogen receptors and androgen receptors: a model system for the study of reproduction.

    abstract：:Estrogen and androgen and their receptors play critical roles in physiological processes such as sexual differentiation and development. Using the available structural models for the human estrogen receptors alpha and beta and androgen receptor as templates, we designed in silico agonist and antagonist models of medak...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.01.047

    authors： Cui J,Shen X,Yan Z,Zhao H,Nagahama Y

    更新日期：2009-02-27 00:00:00

  • Adiponutrin: a multimeric plasma protein.

    abstract：:The interest in adiponutrin stems from adiponutrin variant I148M, which is strongly associated to non-alcoholic fatty liver disease. Adiponutrin has to date been considered to be solely an intracellular protein, with a role in lipid metabolism in liver and adipose tissue. However, a physiologically relevant role for a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.03.078

    authors： Winberg ME,Motlagh MK,Stenkula KG,Holm C,Jones HA

    更新日期：2014-04-18 00:00:00

  • Biocatalysts: beautiful creatures.

    abstract：:The chemical industry has come under increasing pressure to make chemical production more eco-friendly and independent to fossil resources. The development of industrial processes based on micro-organisms can especially help to eliminate the use or the generation of hazardous substances and can support the transition ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2012.07.028

    authors： Saibi W,Abdeljalil S,Masmoudi K,Gargouri A

    更新日期：2012-09-28 00:00:00

  • ZIFIBI: Prediction of DNA binding sites for zinc finger proteins.

    abstract：:The cis-regulatory region of target genes is key elements in the transcriptional regulation of gene expression. Many of these cis-regulatory regions have not been identified by either biological experiments or computational methods. Recently, a few additional C(2)H(2) zinc finger transcription factor binding sites hav...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.02.106

    authors： Cho SY,Chung M,Park M,Park S,Lee YS

    更新日期：2008-05-09 00:00:00

  • Gas6/Axl mediates tumor cell apoptosis, migration and invasion and predicts the clinical outcome of osteosarcoma patients.

    abstract：:Dysregulation of the receptor tyrosine kinase Axl and its ligand Gas6 has been shown to promote multiple tumorigenic processes, as well as to correlate with worse prognosis in many different tumor types. However, studies of Axl expression and function in osteosarcoma have rarely been reported. In this study, we report...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.05.019

    authors： Han J,Tian R,Yong B,Luo C,Tan P,Shen J,Peng T

    更新日期：2013-06-07 00:00:00

  • Combination of 3-methyladenine therapy and Asn-Gly-Arg (NGR)-modified mesoporous silica nanoparticles loaded with temozolomide for glioma therapy in vitro.

    abstract：:Mesoporous silica nanoparticles (MSNPs) of a small diameter were loaded with the anticancer drug temozolomide (TMZ), coated with polydopamine (PDA), and conjugated with Asn-Gly-Arg (NGR) for use in the treatment of glioma. The accumulation of NGR-MSNPs in C6 cells was shown to be higher than that of unmodified MSNPs. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.158

    authors： Zhang P,Tang M,Huang Q,Zhao G,Huang N,Zhang X,Tan Y,Cheng Y

    更新日期：2019-02-05 00:00:00

  • Evidence against GRB10 as the gene responsible for Silver-Russell syndrome.

    abstract：:Recent evidence shows that Silver-Russell syndrome (SRS), the major functional deficit of which is limited growth, both intrauterine and postnatal, is due to a double dose of a gene within 7p11.2-p13 that is normally expressed exclusively from the maternal copy. Of the several growth-related genes in this chromosomal ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5500

    authors： McCann JA,Zheng H,Islam A,Goodyer CG,Polychronakos C

    更新日期：2001-09-07 00:00:00

  • Unraveling the molecular mechanism of BNC105, a phase II clinical trial vascular disrupting agent, provides insights into drug design.

    abstract：:Microtubules are made up of tubulin protein and play a very important part in numerous cellular events of eukaryotic cells, which is why they are seen as attractive targets for tumor chemotherapy. BNC105, a known vascular targeting agent, has entered in phase II clinical trials. It has previously been confirmed that B...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.12.083

    authors： Wang T,Wu C,Wang C,Zhang G,Arnst KE,Yao Y,Zhang Z,Wang Y,Pu D,Li W

    更新日期：2020-02-18 00:00:00

  • Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

    abstract：:CRACR2A protein is described in T cells as an EF-hand-containing modulator of calcium-release-activated calcium (CRAC) channels. Here we sought relevance to calcium entry of endothelial cells. Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.095

    authors： Wilson LA,McKeown L,Tumova S,Li J,Beech DJ

    更新日期：2015-01-02 00:00:00

  • Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin.

    abstract：:Vasohibin is a newly identified negative feedback regulator for angiogenesis. When expressed in cultured human endothelial cells, vasohibin polypeptides were detected in multiple distinct molecular weight forms, suggesting that some proteolytic events may occur within cells or the pericellular milieu. In order to iden...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.01.185

    authors： Sonoda H,Ohta H,Watanabe K,Yamashita H,Kimura H,Sato Y

    更新日期：2006-04-07 00:00:00

  • Uniform amplification of multiple DNAs by emulsion PCR.

    abstract：:When several DNAs are amplified by PCR in one PCR tube, biased amplification is known to occur because amplification efficiency differs from one DNA to another. Therefore, we conducted PCR in the water in oil-emulsion (W/O emulsion) to examine whether the procedure allows the uniform amplification of several DNAs. In ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.037

    authors： Hori M,Fukano H,Suzuki Y

    更新日期：2007-01-12 00:00:00

  • Fast atom bombardment mass spectrometry of bovine proinsulin.

    abstract：:The application of Fast Atom Bombardment Mass Spectrometry to the measurement of small differences in molecular weight is discussed in relation to the spectra of bovine proinsulin and a genetic variant. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(85)80048-6

    authors： Cottrell JS,Frank BH

    更新日期：1985-03-29 00:00:00

  • Identification of tick periviscerokinin, the first neurohormone of Ixodidae: single cell analysis by means of MALDI-TOF/TOF mass spectrometry.

    abstract：:The first peptidergic neurohormone from the ticks Ixodes ricinus and Boophilus microplus has been identified by using a combination of immunocytochemistry and mass spectrometric analysis of single cells. The novel peptide (Ixori-PVK, PALIPFPRV-NH2) shows a high sequence homology with members of the insect periviscerok...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.10.165

    authors： Neupert S,Predel R,Russell WK,Davies R,Pietrantonio PV,Nachman RJ

    更新日期：2005-12-30 00:00:00

  • Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs.

    abstract：:Traditional reconstitution of membrane cytochromes P450 monooxygenase system requires efficient solubilization of both P450 heme enzymes and redox partner NADPH dependent reductase, CPR, either in mixed micellar solution or by incorporation in liposomes. Here we describe a simple alternative approach to assembly of so...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.06.058

    authors： Grinkova YV,Denisov IG,Sligar SG

    更新日期：2010-07-23 00:00:00

  • Prolactin stimulation of protein kinase C activity in the rat hypothalamus.

    abstract：:Stimulation of cultured hypothalamic slices with PRL causes a rapid translocation of a Ca2+/phospholipid dependent protein kinase from the cytosol to the membrane fraction. The translocation of PKC from the cytosol to the membrane occurred at physiological concentrations of PRL with a maximal response occurring at 10(...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80235-9

    authors： DeVito WJ,Stone S,Avakian C

    更新日期：1991-04-30 00:00:00

  • CXCR4 and PTEN are involved in the anti-metastatic regulation of anethole in DU145 prostate cancer cells.

    abstract：:Anethole has been known to have chemopreventive activities as a suppressor of the incidence and multiplicity of both invasive and noninvasive carcinomas. The goal of this study was to understand the anti-metastatic effect of anethole through C-X-C chemokine receptor type 4 (CXCR4)/tumor suppressor phosphatase and tens...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.121

    authors： Rhee YH,Chung PS,Kim SH,Ahn JC

    更新日期：2014-05-16 00:00:00

  • Chemical and thermal sensitivity of medaka TRPA1 analyzed in heterologous expression system.

    abstract：:TRPA1 of insects and several tetrapod vertebrates except for those of rodents have been reported to be activated by noxious chemicals and also by high temperature with a relatively clear threshold. We previously analyzed the characteristics of two TRPA1 paralogs of zebrafish (zTRPA1a, b) and demonstrated that zTRPA1a ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.057

    authors： Oda M,Saito K,Hatta S,Kubo Y,Saitoh O

    更新日期：2017-12-09 00:00:00

  • Uncoupling of oxidative phosphorylation in rat liver mitochondria following the administration of dimethyl sulphoxide.

    abstract：:A single intraperitoneal injection of 275 mg of dimethyl sulphoxide (DMSO) to rats (100-125 g body weight) effectively uncouples oxidative phosphorylation in liver mitochondria during the period from 2 hr to 5 day post-injection. Higher doses of DMSO are inhibitory to mitochondrial respiration. DMSO has however no unc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91299-8

    authors： Mhatre SS,Chetty KG,Pradhan DS

    更新日期：1983-01-14 00:00:00

  • Peroxiredoxin II is essential for preventing hemolytic anemia from oxidative stress through maintaining hemoglobin stability.

    abstract：:The pathophysiology of oxidative hemolytic anemia is closely associated with hemoglobin (Hb) stability; however, the mechanism of how Hb maintains its stability under oxidative stress conditions of red blood cells (RBCs) carrying high levels of oxygen is unknown. Here, we investigated the potential role of peroxiredox...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.08.113

    authors： Han YH,Kim SU,Kwon TH,Lee DS,Ha HL,Park DS,Woo EJ,Lee SH,Kim JM,Chae HB,Lee SY,Kim BY,Yoon DY,Rhee SG,Fibach E,Yu DY

    更新日期：2012-09-28 00:00:00

  • Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme.

    abstract：:VMA1 translational product undergoes excision of a 50-kDa intervening segment (VDE: VMA1-derived endonuclease) and religation of the flanking regions to create a 69-kDa catalytic subunit of vacuolar membrane H+-ATPase. VDEs conjugated with polypeptides at both N- and C-terminal ends were expressed in Escherichia coli ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0826

    authors： Kawasaki M,Makino S,Matsuzawa H,Satow Y,Ohya Y,Anraku Y

    更新日期：1996-05-24 00:00:00

  • Activation of the p21(Waf1/Cip1) promoter by the ets oncogene family transcription factor E1AF.

    abstract：:p21(Waf1/Cip1) is one of the key regulatory proteins in cell cycle, terminal differentiation, and apoptosis. Its promoter was shown to be transactivated by the wild-type p53 protein as well as in a p53-independent manner. In this report, we demonstrate that E1AF, an ets-related transcription factor, activates the huma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6909

    authors： Funaoka K,Shindoh M,Yoshida K,Hanzawa M,Hida K,Nishikata S,Totsuka Y,Fujinaga K

    更新日期：1997-07-09 00:00:00

  • Modulation of fluoropyrimidine metabolism by chlorpromazine.

    abstract：:Chlorpromazine is a potent inhibitor of calmodulin and can alter the accumulation, metabolism, and incorporation into RNA of several fluoropyrimidine drugs. Using S-180 murine sarcoma cells, the accumulation of the base Fluorouracil and its metabolism to ribonucleotides is enhanced by chlorpromazine treatment, as meas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80596-4

    authors： Tseng A Jr,Brooks M,Cadman E

    更新日期：1986-07-31 00:00:00