Prolactin stimulation of protein kinase C activity in the rat hypothalamus.

abstract：:We report the discovery, semi-purification and characterization of a novel Ca2+/calmodulin-dependent protein kinase (peak I kinase) using syntide 2 as a substrate from the rabbit heart. In the study of dependence of peak I kinase on the concentration of calmodulin, half-maximal activation was obtained at approx. 2.0 x...

journal_title：Biochemical and biophysical research communications

authors： Uemura A,Okazaki K,Takesue H,Matsubara T,Hidaka H

更新日期：1995-06-15 00:00:00

abstract：:We have identified a novel reaction in which acetaldehyde promotes rat hepatic cytosolic catalysis of O2 consumption coupled with glutathione oxidation without apparent release of activated forms of O2. Acetaldehyde is not consumed in the reaction. The reaction (O2 consumption or oxidized glutathione production) is sa...

journal_title：Biochemical and biophysical research communications

authors： Lyon I,Kaplowitz N

更新日期：1985-06-28 00:00:00

abstract：:Increased ubiquitin-specific protease 5 (USP5) has been associated with tumorigenesis of malignancy including glioblastoma, melanoma and hepatocellular carcinoma. However, the role of USP5 in tumorigenesis of pancreatic ductal adenocarcinoma (PDAC) has not been studied yet. In this study, we demonstrated that USP5 was...

journal_title：Biochemical and biophysical research communications

authors： Li XY,Wu HY,Mao XF,Jiang LX,Wang YX

更新日期：2017-10-07 00:00:00

abstract：:The genomic sequence of the halophilic archaeon Halobacterium NRC-1 has been analyzed by the Z curve method. The Z curve is a three-dimensional curve that uniquely represents a given DNA sequence. Based on the known behaviors of the Z curves for the archaea whose replication origins have been identified, the analysis ...

journal_title：Biochemical and biophysical research communications

authors： Zhang R,Zhang CT

更新日期：2003-03-21 00:00:00

abstract：:We have previously reported that endothelin-1 (ET-1) stimulates interleukin-6 (IL-6), a potent bone resorptive agent, through p44/p42 mitogen-activated protein (MAP) kinase and p38 MAP kinase in osteoblast-like MC3T3-E1 cells. In the present study, we investigated the involvement of Rho-kinase in the ET-1-stimulated I...

journal_title：Biochemical and biophysical research communications

authors： Tokuda H,Hanai Y,Matsushima-Nishiwaki R,Yamauchi J,Doi T,Harada A,Takai S,Kozawa O

更新日期：2007-11-03 00:00:00

abstract：:Pyruvate dehydrogenase kinase (PDK) specifically phosphorylates the E1alpha subunit of the pyruvate dehydrogenase complex (PDC). Sequence analysis of cloned PDKs led to the proposal that they are mechanistically related to prokaryotic 2-component His-kinases. The reaction mechanism of protein His-kinases involves auto...

journal_title：Biochemical and biophysical research communications

authors： Mooney BP,David NR,Thelen JJ,Miernyk JA,Randall DD

更新日期：2000-01-19 00:00:00

abstract：:A study was made of the effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes. 1 microg/ml of the venom made the resting plasma membrane potential more negative in cells voltage-clamped at -60 mV. The effect was potentially due to the closure of one or seve...

journal_title：Biochemical and biophysical research communications

authors： Parodi J,Romero F,Miledi R,Martínez-Torres A

更新日期：2008-10-31 00:00:00

abstract：:Antithrombin III (ATIII) performs a critical anticoagulant function by precluding the activation of blood clotting proteinases, aided by its two cofactors, heparin and heparan sulfate. Though several studies have been carried out on physiological, biochemical and structural perspectives on ATIII, so far there are limi...

journal_title：Biochemical and biophysical research communications

authors： Kumar A,Bhandari A,Sarde SJ,Goswami C

更新日期：2013-11-01 00:00:00

abstract：:The gene encoding rat cystatin S (Cys S), a salivary gland-specific secretory protein, has CAAT and TATA boxes upstream of the inititation codon (Cox and Shaw, 1992), and contains regions that resemble those of other hormonally responsive eukaryotic genes. The 5'-flanking sequence of the rat Cys S gene has a potential...

journal_title：Biochemical and biophysical research communications

authors： Shaw PA,Chaparro O

更新日期：1999-08-11 00:00:00

abstract：:Nonalcoholic fatty liver disease (NAFLD) is featured by hepatic steatosis, insulin resistance, lipid deposition and inflammation. However, the pathogenic mechanism of NAFLD is still poorly understood. Dual-specificity phosphatase 16 (DUSP16), a c-Jun N-terminal kinase-specific phosphatase, has been reported to negativ...

journal_title：Biochemical and biophysical research communications

authors： Wu YK,Hu LF,Lou DS,Wang BC,Tan J

更新日期：2020-03-26 00:00:00

abstract：:Irradiation induces severe damage in the hematopoietic system, which leads to bone marrow hyperplasia, pancytopenia, and aggravated tissue formation in bone marrow. Studies have shown that Toll-like receptor 4 (TLR4) has a protective effect against irradiation, but the underlying mechanism remains unclear. In this stu...

journal_title：Biochemical and biophysical research communications

authors： Sun Z,Wu K,Lin Q,Fei H,Jiang H,Chen T,Yuan Y

更新日期：2019-12-03 00:00:00

abstract：:miRNA is increasingly being recognized as a key regulator of metabolism in animals. A wealth of evidence has suggested that miRNA mainly binds 3' UTR of mRNA and modulates the cell activities via repressing the mRNA translation. However, as the translation initiates at 5' UTR, cis elements like upstream open reading f...

journal_title：Biochemical and biophysical research communications

authors： Hu W,Wang T,Yang Y,Zheng S

更新日期：2014-09-12 00:00:00

abstract：:Neurodegenerative diseases are characterized by distinctive neuropathological alterations, including the cerebral accumulation of misfolded protein aggregates, neuroinflammation, synaptic dysfunction, and neuronal loss, along with behavioral impairments. Traumatic brain injury (TBI) is believed to be an important risk...

journal_title：Biochemical and biophysical research communications

authors： Edwards G 3rd,Moreno-Gonzalez I,Soto C

更新日期：2017-02-19 00:00:00

abstract：:The mRNA coding for thyroglobulin in cattle homozygous for an autosomal recessive defect of thyroglobulin synthesis was investigated using a recombinant plasmid containing bovine mRNA coding sequence. Total RNA preparations from goiter contained one third of the thyroglobulin mRNA sequences found in normal thyroid tis...

journal_title：Biochemical and biophysical research communications

authors： Ricketts MH,Vandenplas S,van der Walt M,van Jaarsveld PP,Bester AJ,Boyd CD

更新日期：1985-01-16 00:00:00

abstract：:Kinetic analyses were performed to understand the mechanism of hyperoxic induced inhibition of prostacyclin synthesis by human umbilical arteries. Brief exposure of arterial segments to oxygen resulted in over 30% decrease in Vmax of cyclooxygenase in treated vessels. In contrast, cyclooxygenase from hypoxic arterial ...

journal_title：Biochemical and biophysical research communications

authors： Yamaja Setty BN,Walenga RW,Stuart MJ

更新日期：1984-11-30 00:00:00

abstract：:The immunoglobulin G of a polyclonal antiserum (pIgG) from a patient with insulin resistance and hypoglycemia was tested for its ability to inhibit insulin binding and to affect the autophosphorylation of partially-purified insulin receptors extracted from rat liver membranes. pIgG, when added 4 hr prior to insulin, i...

journal_title：Biochemical and biophysical research communications

authors： Cordera R,Gherzi R,De Pirro R,Rossetti L,Freidenberg GR,Andraghetti G,Lauro R,Adezati L

更新日期：1985-11-15 00:00:00

abstract：:Proteins modified by oxidants are rapidly degraded by intracellular proteases. Oxidatively modified superoxide dismutase (Ox-SOD) was degraded 2-8 times faster at both acidic and alkaline pH than the native protein in bovine cardiac tissue extracts. At acidic pH, Ox-SOD hydrolysis was stimulated by ATP and by non-hydr...

journal_title：Biochemical and biophysical research communications

authors： Strack PR,Waxman L,Fagan JM

更新日期：1996-02-15 00:00:00

abstract：:The Frizzled-type cysteine-rich domain (CRD) is a binding motif for soluble-type glycoprotein WNTs, which play key roles in embryogenesis and carcinogenesis. Here, we have cloned and characterized a novel gene MFRP, encoding a type II transmembrane protein with CRD. In addition to CRD, two tandem-repeats containing th...

journal_title：Biochemical and biophysical research communications

authors： Katoh M

更新日期：2001-03-23 00:00:00

abstract：:Our previous data from a human leukemic cell line made resistant to the nucleoside analog (NA) 9-β-D-arabinofuranosylguanine (AraG) revealed a massive upregulation of fetal hemoglobin (HbF) genes and the ABCB1 gene coding for the multidrug resistance P-glycoprotein (P-gp). The expression of these genes is regulated th...

journal_title：Biochemical and biophysical research communications

authors： Fyrberg A,Skoglund K,Wolk M,Lotfi K

更新日期：2012-10-26 00:00:00

abstract：:Because the expression of EBNA1 is prevalent in all EBV-associated tumors, it has become one of the most attractive drug targets for the discovery of anti-EBV compounds. In a cell-based reporter system, EBNA1 consistently upregulated the transcription of an oriP-Luc mini-EBV episome by 6- to 8-fold. The treatment of c...

journal_title：Biochemical and biophysical research communications

authors： Chen YL,Tsai HL,Peng CW

更新日期：2012-01-20 00:00:00

abstract：:Rap1GAP (for Rap1 GTPase Activating Protein) is an 89 kD protein that highly stimulates the intrinsic GTPase activity of the small GTP binding protein Rap1. It has been shown that Rap1GAP is phosphorylated in vitro by purified p34cdc2 kinase, which regulates the G2/M transition of the cell cycle. In this work, we have...

journal_title：Biochemical and biophysical research communications

authors： Janoueix-Lerosey I,Fontenay M,Tobelem G,Tavitian A,Polakis P,de Gunzburg J

更新日期：1994-07-29 00:00:00

abstract：:The cytosolic sulfotransferases (SULTs) in vertebrates catalyze the sulfonation of endogenous thyroid/steroid hormones and catecholamine neurotransmitters, as well as a variety of xenobiotics, using 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as the sulfonate donor. In this study, we determined the structures of SULT...

journal_title：Biochemical and biophysical research communications

authors： Lu J,Li H,Zhang J,Li M,Liu MY,An X,Liu MC,Chang W

更新日期：2010-05-28 00:00:00

abstract：:ERK5, a member of the mitogen activated protein kinase, expressed in the kidneys was smaller (∼80kDa) in apparent molecular mass compared to other organs (∼120kDa). A blocking peptide experiment confirmed that the ∼80kDa detected on Western blots was a specific band detected by the anti-ERK5 antibody. Expression of th...

journal_title：Biochemical and biophysical research communications

authors： Kawakami T,Park SW,Kaku R,Yang J

更新日期：2012-02-24 00:00:00

abstract：:A 1763-bp cDNA for rat liver bilirubin UDP-glucuronosyltransferase (UDPGT) was isolated. Bilirubin UDPGT activity was demonstrated by transfection of the pcDL1 vector carrying the cDNA into COS7 monkey kidney cells. The cDNA shares an identical 913-bp sequence (corresponding to the C-terminal 247 amino acid residues) ...

journal_title：Biochemical and biophysical research communications

authors： Sato H,Koiwai O,Tanabe K,Kashiwamata S

更新日期：1990-05-31 00:00:00

abstract：:Metallothionein (MT) is believed to be a sensitive and effective "biomarker" for monitoring metal contamination in fish. Comparison of amino acid sequences between flounder MT and trout MT revealed that there is a conserved region at their N-terminals. Using oligonucleotides derived from this conserved region, reverse...

journal_title：Biochemical and biophysical research communications

authors： Chan KM

更新日期：1994-11-30 00:00:00

abstract：:A chemical inhibitor library of 84 compounds was screened to investigate the signaling pathway(s) leading to activation of Nrf2 in response to nitric oxide (NO). We identified the protein kinase C delta (PKCdelta) inhibitor rottlerin as the only compound that reduced NO-induced ARE-luciferase reporter activity and dim...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Hung AC,Ng PY,Nakayama K,Hu Y,Li B,Porter AG,Dhakshinamoorthy S

更新日期：2009-09-04 00:00:00

abstract：:RNF8 is a nuclear protein having an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-finger (RF) domain. Depletion of RNF8 caused cell growth inhibition and cell cycle arrest at not only S but also G2/M phases. In addition, cell death was frequently observed in RNF8-depleted cells. Analyses of time-la...

journal_title：Biochemical and biophysical research communications

authors： Yoshioka T,Kimura M,Saio M,Era S,Okano Y

更新日期：2011-06-24 00:00:00

abstract：:PSI domains are cysteine-rich modules found in extracellular fragments of hundreds of signaling proteins, including plexins, semaphorins, integrins, and attractins. Here, we report the solution structure of the PSI domain from the human Met receptor, a receptor tyrosine kinase critical for proliferation, motility, and...

journal_title：Biochemical and biophysical research communications

authors： Kozlov G,Perreault A,Schrag JD,Park M,Cygler M,Gehring K,Ekiel I

更新日期：2004-08-13 00:00:00

abstract：:Binding of [3H]-staurosporine to different protein kinases was time-dependent, reversible and saturable. Scatchard analysis of saturation isotherms indicated one class of binding sites for [3H]-staurosporine with dissociation constants (KD) of 9.6, 2.0, 3.0 and 7.4 nM for protein kinase C, cAMP-dependent protein kinas...

journal_title：Biochemical and biophysical research communications

authors： Herbert JM,Seban E,Maffrand JP

更新日期：1990-08-31 00:00:00

abstract：:It has been well documented that tumor suppressor p53 is mutated in about 50% of all human tumors. p53 status might be one of the critical determinants for the chemo-sensitivity of human tumors. In the present study, we have found that p53 family member p73 as well as 14-3-3sigma is down-regulated in response to adria...

journal_title：Biochemical and biophysical research communications

authors： Sang M,Li Y,Ozaki T,Ono S,Ando K,Yamamoto H,Koda T,Geng C,Nakagawara A

更新日期：2006-08-18 00:00:00