Abstract:
:A study was made of the effects of the venom of the Chilean spider Latrodectus mactans on endogenous ion-currents of Xenopus laevis oocytes. 1 microg/ml of the venom made the resting plasma membrane potential more negative in cells voltage-clamped at -60 mV. The effect was potentially due to the closure of one or several conductances that were investigated further. Thus, we determined the effects of the venom on the following endogenous ionic-currents: (a) voltage-activated potassium currents, (b) voltage-activated chloride-currents, and (c) calcium-dependent chloride-currents (Tout). The results suggest that the venom exerts its action mainly on a transient outward potassium-current that is probably mediated by a Kv channel homologous to shaker. Consistent with the electrophysiological evidence we detected the expression of the mRNA coding for xKv1.1 in the oocytes.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Parodi J,Romero F,Miledi R,Martínez-Torres Adoi
10.1016/j.bbrc.2008.08.070subject
Has Abstractpub_date
2008-10-31 00:00:00pages
571-5issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(08)01582-9journal_volume
375pub_type
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