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Expression of a long variant of CRACR2A that belongs to the Rab GTPase protein family in endothelial cells.

Abstract：

:CRACR2A protein is described in T cells as an EF-hand-containing modulator of calcium-release-activated calcium (CRAC) channels. Here we sought relevance to calcium entry of endothelial cells. Unexpectedly, short interfering RNA designed to deplete CRACR2A had no effect on CRAC channels in endothelial cells but reduced the abundance of a protein with about twice the mass of CRACR2A. Reference to gene sequence data indicated the potential for a variant transcript encoding a C-terminal Rab GTPase extension of CRACR2A. Full-length cloning demonstrated expression of the long variant in endothelial cells. It was designated CRACR2A-L. Sequence analysis suggested it to be a previously unrecognised member of the Rab GTPase family. It made a positive contribution to endothelial tube formation. The data suggest that endothelial cells contain a long variant of CRACR2A which is an EF-hand-containing Rab protein that lacks impact on CRAC channels.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wilson LA,McKeown L,Tumova S,Li J,Beech DJ

doi

10.1016/j.bbrc.2014.11.095

subject

Has Abstract

pub_date

2015-01-02 00:00:00

pages

398-402

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)02136-6

journal_volume

456

pub_type

杂志文章

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