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Folding-dependent in vitro protein splicing of the Saccharomyces cerevisiae VMA1 protozyme.

Abstract：

:VMA1 translational product undergoes excision of a 50-kDa intervening segment (VDE: VMA1-derived endonuclease) and religation of the flanking regions to create a 69-kDa catalytic subunit of vacuolar membrane H+-ATPase. VDEs conjugated with polypeptides at both N- and C-terminal ends were expressed in Escherichia coli and examined for their ability to catalyze self-splicing. Processed VDE was found in soluble pools, while unspliced precursors accumulated in insoluble pools, forming inclusion bodies. We demonstrate in vitro protein splicing by refolding of the denatured precursor molecules. The processing reaction efficiently occurs with the purified precursor peptide. VDE bracketed by only 6 proximal and 4 distal amino acids is autocatalytically processed.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kawasaki M,Makino S,Matsuzawa H,Satow Y,Ohya Y,Anraku Y

doi

10.1006/bbrc.1996.0826

subject

Has Abstract

pub_date

1996-05-24 00:00:00

pages

827-32

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X96908268

journal_volume

222

pub_type

杂志文章

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