Alpha-bulnesene, a novel PAF receptor antagonist isolated from Pogostemon cablin.


:Alpha-bulnesene is a sesquiterpenoid isolated from the water extract of Pogostemon cablin. It showed a potent and concentration-dependent inhibitory effect on platelet-activating factor (PAF) and arachidonic acid (AA) induced rabbit platelet aggregation. In a radioligand binding assay for the PAF receptor, alpha-bulnesene competitively inhibited [(3)H]PAF binding to the PAF receptor with an IC(50) value of 17.62+/-5.68microM. alpha-Bulnesene also dose-dependently inhibited PAF-induced intracellular Ca(2+) increase in fluo-3/AM-loaded platelets (IC(50) values of 19.62+/-1.32microM). Furthermore, alpha-bulnesene inhibited AA-induced thromboxane B(2) (TXB(2)) formation and prostaglandin E(2) (PGE(2)) formation. These results indicate that the inhibitory effect of alpha-bulnesene on platelet aggregation was due to a dual activity; specifically the chemical blocked PAF-induced intracellular signal transduction and interfered with cyclooxygenase activity, which resulted in a decrease in thromboxane formation. This study is the first to demonstrate that alpha-bulnesene is a PAF receptor antagonist as well as an anti-platelet aggregation agent.


Hsu HC,Yang WC,Tsai WJ,Chen CC,Huang HY,Tsai YC




Has Abstract


2006-07-07 00:00:00














  • Role of FOXA and Sp1 in mitochondrial acylcarnitine carrier gene expression in different cell lines.

    abstract::This study investigates the transcriptional role of the human mitochondrial carnitine/acylcarnitine carrier (CAC) proximal promoter. Through deletion analysis, an activation domain (-334/-80 bp) was identified which contains FOXA and Sp1 active sites. The wild-type (but not mutated) -334/-80 bp region of the CAC gene ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Convertini P,Infantino V,Bisaccia F,Palmieri F,Iacobazzi V

    更新日期:2011-01-07 00:00:00

  • Sphingolipids increase calcium concentration in isolated rat liver nuclei.

    abstract::The sphingolipids, sphingosine (SPH), sphingosylphosphorylcholine (SPC) and psycosine induce a rapid and transient rise in nuclear free Ca2+ concentration in a dose dependent manner. To determine whether these sphingolipids act by a IP3-dependent pathway, we tested the increase of Ca2+ in the presence of heparin, an a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Catalán RE,Miguel BG,Calcerrada MC,Ruiz S,Martínez AM

    更新日期:1997-09-18 00:00:00

  • Salvianolic acid A protects human SH-SY5Y neuroblastoma cells against H₂O₂-induced injury by increasing stress tolerance ability.

    abstract::Salvianolic acid A (Sal A) is a polyphenol extracted from the root of the Salvia miltiorrhiza bunge. Hydrogen peroxide (H(2)O(2)) is a major reactive oxygen species (ROS), which has been implicated in stroke and other neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. In this study, we ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang HA,Gao M,Zhang L,Zhao Y,Shi LL,Chen BN,Wang YH,Wang SB,Du GH

    更新日期:2012-05-11 00:00:00

  • An investigation on reduction process of cucumber ascorbate oxidase.

    abstract::Reduction process of cucumber ascorbate oxidase with L-ascorbate was investigated in detail through absorption and electron paramagnetic resonance (EPR) spectra under anaerobic condition. One of the three type I coppers (the type I copper which is oxidized rapidly (Sakurai, T. et al. (1985) Biochem. Biophys. Res. Comm...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakurai T,Sawada S,Suzuki S,Nakahara A

    更新日期:1986-03-13 00:00:00

  • Active-site probes of carnitine acyltransferases. Inhibition of carnitine acetyltransferase by hemiacetylcarnitinium, a reaction intermediate analogue.

    abstract::Hemiacetylcarnitinium (2S,6R:2R,65)-6-carboxymethyl-2-hydroxy-2,4,4- trimethylmorpholinium) chloride is a relatively potent competitive inhibitor (Ki = 0.89 mM) of pigeon breast carnitine acetyltransferase (CAT) and of the crude rat liver CAT (Ki = 4.72 mM) but is neither an inhibitor nor an effective substrate for pu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gandour RD,Colucci WJ,Stelly TC,Brady PS,Brady LJ

    更新日期:1986-07-31 00:00:00

  • p70s6 kinase is a functional target of insulin activated Akt cell-survival signaling.

    abstract::Insulin administration attenuates cardiac ischemia-reperfusion apoptosis via activation of Akt-mediated cell-survival signaling. As p70s6 kinase is a cognate Akt-mediated phosphorylation target we evaluated whether p70s6 kinase activation is a functional requirement in insulin-mediated cell survival program during pos...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jonassen AK,Mjøs OD,Sack MN

    更新日期:2004-02-27 00:00:00

  • Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells.

    abstract::Cepharanthine is a medicinal plant-derived natural compound which possesses potent anti-cancer properties. However, there is little report about its effects on lung cancer cells. In this study, we investigated the effects of cepharanthine on the cell viability and apoptosis in human non-small-cell lung cancer H1299 an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hua P,Sun M,Zhang G,Zhang Y,Tian X,Li X,Cui R,Zhang X

    更新日期:2015-05-01 00:00:00

  • The effect of interaction between human urokinase and its competitive inhibitor, N alpha-benzoyl-L-arginine amide, on reduction of a specific SS bond related to enzymatic activity.

    abstract::The 55- (H-UK) and 36-kDa forms (L-UK) of human urinary urokinase lost most of esterase activity toward acetyl-glycyl-L-lysine methyl ester upon reductive cleavage of 3 SS bonds with dithiothreitol in the presence of the competitive inhibitor, N alpha-benzoyl-L-arginine amide (BAA), bound to polyacrylyl azide with C16...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Miwa N,Obata Y,Suzuki A

    更新日期:1983-04-29 00:00:00

  • Bisulfite conversion-specific and methylation-specific PCR: a sensitive technique for accurate evaluation of CpG methylation.

    abstract::Methylation-specific PCR (MSP) is frequently used to distinguish methylated alleles in the genome. Sequences that have been incompletely converted during bisulfite treatment are frequently co-amplified during MSP. For accurate MSP, it is important to detect methylated sequences in a background of unconverted DNA with ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sasaki M,Anast J,Bassett W,Kawakami T,Sakuragi N,Dahiya R

    更新日期:2003-09-19 00:00:00

  • ATP is a cryptic binder of TDP-43 RRM domains to enhance stability and inhibit ALS/AD-associated fibrillation.

    abstract::ATP is the universal energy currency for all cells but has cellular concentrations of 2-12 mM, much higher than required for its classic functions. RNA-recognition motif (RRM) constitutes one of the most abundant domains in eukaryotes and most heterogeneous nuclear ribonucleoproteins (hnRNP) contain RRM domains which ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dang M,Kang J,Lim L,Li Y,Wang L,Song J

    更新日期:2020-01-29 00:00:00

  • A common peptide stretch among enzymes localized to the Golgi apparatus: structural similarity of Golgi-associated glycosyltransferases.

    abstract::A common peptide motif has been discovered among a series of Golgi-localized glycosyltransferases. The peptide stretch, (Ser/Thr)-X-(Glu/Gln)-(Arg/Lys), always occurs near a hydrophobic domain close to the N-terminus of these enzymes which is believed to anchor them to the membrane lipid bilayer (Paulson and Colley, J...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bendiak B

    更新日期:1990-07-31 00:00:00

  • High glucose induced VEGF expression via PKC and ERK in glomerular podocytes.

    abstract::Podocytes are the major site of vascular endothelial growth factor (VEGF) production in the kidney, and up-regulation of VEGF plays a critical role in the progression of diabetic nephropathy. Using a differentiated mouse podocyte cell line, we investigated the roles of protein kinase C (PKC) and extracellular signal-r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hoshi S,Nomoto K,Kuromitsu J,Tomari S,Nagata M

    更新日期:2002-01-11 00:00:00

  • Macrophage and endothelial cell nitric oxide synthesis: cell-type selective inhibition by NG-aminoarginine, NG-nitroarginine and NG-methylarginine.

    abstract::Many cell types are known to synthesize nitric oxide (NO.) from L-arginine. There appear to be at least two forms of NO. synthase: an inducible, tetrahydrobiopterin- and flavin-dependent activity exemplified by the macrophage enzyme and a constitutive, Ca+(+)-dependent activity exemplified by the endothelial cell enzy...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gross SS,Stuehr DJ,Aisaka K,Jaffe EA,Levi R,Griffith OW

    更新日期:1990-07-16 00:00:00

  • Oxidation-reduction potential and paraoxonase-arylesterase activity in trauma patients.

    abstract::The amount of oxidative stress in severely traumatized patients is usually based on various individual parameters such as total antioxidants and lipid peroxidation. Serial measurements of plasma oxidation-reduction potential (ORP) in severely traumatized patients as a simple mean of assessing overall oxidative stress ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rael LT,Bar-Or R,Aumann RM,Slone DS,Mains CW,Bar-Or D

    更新日期:2007-09-21 00:00:00

  • Biocatalysts: beautiful creatures.

    abstract::The chemical industry has come under increasing pressure to make chemical production more eco-friendly and independent to fossil resources. The development of industrial processes based on micro-organisms can especially help to eliminate the use or the generation of hazardous substances and can support the transition ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Saibi W,Abdeljalil S,Masmoudi K,Gargouri A

    更新日期:2012-09-28 00:00:00

  • Duplicated cytoglobin genes in teleost fishes.

    abstract::Cytoglobin is a recently discovered myoglobin-related O2-binding protein of vertebrates with uncertain function. It occurs as single-copy gene in mammals. Here, we demonstrate the presence of two paralogous cytoglobin genes (Cygb-1 and Cygb-2) in the teleost fishes Danio rerio, Oryzias latipes, Tetraodon nigroviridis,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fuchs C,Luckhardt A,Gerlach F,Burmester T,Hankeln T

    更新日期:2005-11-11 00:00:00

  • Some fused heterocyclic compounds as eukaryotic topoisomerase II inhibitors.

    abstract::Our previously synthesized 37 compounds, which are 2,5,6-substituted benzoxazole, benzimidazole, benzothiazole, and oxazolo(4,5-b)pyridine derivatives, were tested for their eukaryotic DNA topoisomerase II inhibitory activity in cell free system and 28 were found to inhibit the topoisomerase II at an initial concentra...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pinar A,Yurdakul P,Yildiz I,Temiz-Arpaci O,Acan NL,Aki-Sener E,Yalcin I

    更新日期:2004-04-30 00:00:00

  • Reduction in number of sarcolemmal KATP channels slows cardiac action potential duration shortening under hypoxia.

    abstract::The cardiovascular system operates under demands ranging from conditions of rest to extreme stress. One mechanism of cardiac stress tolerance is action potential duration shortening driven by ATP-sensitive potassium (K(ATP)) channels. K(ATP) channel expression has a significant physiologic impact on action potential d...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhu Z,Burnett CM,Maksymov G,Stepniak E,Sierra A,Subbotina E,Anderson ME,Coetzee WA,Hodgson-Zingman DM,Zingman LV

    更新日期:2011-12-02 00:00:00

  • The pkI gene encoding pyruvate kinase I links to the luxZ gene which enhances bioluminescence of the lux operon from Photobacterium leiognathi.

    abstract::Partial 3'-end nucleotide sequence of the pkI gene (GenBank accession No. AF019143) from Photobacterium leiognathi ATCC 25521 has been determined, and the encoded pyruvate kinase I is deduced. Pyruvate kinase I is the key enzyme of glycolysis, which converts phosphoenol pyruvate to pyruvate. Alignment and comparison o...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin JW,Lu HC,Chen HY,Weng SF

    更新日期:1997-10-09 00:00:00

  • Bioactive synthetic peptide of NDF/heregulin.

    abstract::A folded synthetic peptide analog of NDF (NDF5), which has the 52 amino acid EGF-like domain of NDF alpha 2, has been characterized. The folded peptide stimulates tyrosine phosphorylation of Her2, Her3 and Her4 in breast cancer cells and competes with low affinity with full-length NDF alpha 2 for binding to the cells,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kita Y,Mayer J,Zamborelli T,Hara S,Rohde M,Watson E,Koski R,Ratzkin B,Nicolson M

    更新日期:1995-05-16 00:00:00

  • Identification of SIV Nef CD8(+) T cell epitopes restricted by a MHC class I haplotype associated with lower viral loads in a macaque AIDS model.

    abstract::Virus-specific CD8(+) T-cell responses are crucial for the control of human immunodeficiency virus (HIV) and simian immunodeficiency virus (SIV) replication. Multiple studies on HIV-infected individuals and SIV-infected macaques have indicated association of several major histocompatibility complex class I (MHC-I) gen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nomura T,Yamamoto H,Takahashi N,Naruse TK,Kimura A,Matano T

    更新日期:2014-07-25 00:00:00

  • X-ray structure of a protease-resistant mutant form of human galectin-9 having two carbohydrate recognition domains with a metal-binding site.

    abstract::Galectin-9 (G9) is a tandem-repeat type β-galactoside-specific animal lectin having N-terminal and C-terminal carbohydrate recognition domains (N-CRD and C-CRD, respectively) joined by a linker peptide that is involved in the immune system. G9 is divalent in glycan binding, and structural information about the spatial...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yoshida H,Nishi N,Wada K,Nakamura T,Hirashima M,Kuwabara N,Kato R,Kamitori S

    更新日期:2017-09-02 00:00:00

  • Hypertonic induction of COX2 expression requires TonEBP/NFAT5 in renal epithelial cells.

    abstract::TonEBP/NFAT5 transcription factor is a master regulator of genes involved in osmoprotection. Cyclooxygenase 2 (COX2) has been reported to be a cytoprotective molecule in the inner renal medulla, where cells are physiologically exposed to the highest osmolality of the body. Our aim was to study whether COX2 expression ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Favale NO,Casali CI,Lepera LG,Pescio LG,Fernández-Tome MC

    更新日期:2009-04-10 00:00:00

  • GRK3 regulation during CRF- and urocortin-induced CRF1 receptor desensitization.

    abstract::The EC(50) values for concentration-dependent stimulation of cAMP accumulation by CRF (1.3nM) and urocortin (1.0nM) were equivalent in human retinoblastoma Y79 cells. The time course and magnitude of CRF- and urocortin-induced CRF(1) receptor desensitization were similar. A significant 3-fold increase in GRK3, but not...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dautzenberg FM,Wille S,Braun S,Hauger RL

    更新日期:2002-11-01 00:00:00

  • Sequence of the gene, amoB, for the 43-kDa polypeptide of ammonia monoxygenase of Nitrosomonas europaea.

    abstract::The sequence for the 3' portion of amoB, a gene encoding a 43-kDa polypeptide component ammonia monooxygenase of Nitrosomonas europaea, is presented. The derived polypeptide has no homology with other known proteins. AmoA and amoB are the only open reading frames in the putative amo operon. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bergmann DJ,Hooper AB

    更新日期:1994-10-28 00:00:00

  • Genistein, a tyrosine kinase inhibitor, blocks the cell cycle progression but not Ca2+ influx induced by BAY K8644 in FRTL-5 cells.

    abstract::IGF-I, when added to TSH-primed FRTL-5 cells, induces a long lasting Ca2+ influx and then DNA synthesis. Ca2+ channel agonist, BAYK 8644, is capable to mimic these effects on cell proliferation. We studied the effect of genistein, a specific tyrosine kinase inhibitor, on BAY K8644- or IGF-I- induced cell cycle progres...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takano T,Takada K,Tada H,Nishiyama S,Amino N

    更新日期:1993-02-15 00:00:00

  • The sterol-binding antibiotic nystatin inhibits entry of non-opsonized Leishmania donovani into macrophages.

    abstract::Leishmania donovani is an obligate intracellular parasite that infects macrophages of the vertebrate host resulting in visceral leishmaniasis in humans, a major public health problem worldwide. The molecular mechanisms involved in internalization of Leishmania are still poorly characterized. We report here that choles...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tewary P,Veena K,Pucadyil TJ,Chattopadhyay A,Madhubala R

    更新日期:2006-01-13 00:00:00

  • Molecular cloning of the human volume-sensitive chloride conductance regulatory protein, pICln, from ocular ciliary epithelium.

    abstract::Chloride channels in the ocular ciliary epithelium are believed to play a key role in aqueous humor formation. We isolated a cDNA clone from a lambda Uni-ZAP cDNA library of human nonpigmented ciliary epithelial (NPE) cells encoding the swelling-induced chloride channel/channel regulator pICln. The human clone contain...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Anguita J,Chalfant ML,Civan MM,Coca-Prados M

    更新日期:1995-03-08 00:00:00

  • Variations in ADP-ribosylation of nuclear scaffold proteins during the HeLa cell cycle.

    abstract::Cell cycle variations in ADP-ribosylation of nuclear scaffold proteins were determined. Nuclei of synchronized cells were isolated and labeled with [32P]NAD before nuclear scaffolds were obtained by digestion of DNA with DNase I and extraction of proteins with 2M NaCl. Autoradiograms revealed the three groups of "lami...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Adolph KW,Song MK

    更新日期:1985-01-31 00:00:00

  • Activation of the Sendai virus fusion protein by receptor binding.

    abstract::2,3 Dehydro-2-deoxy-N-acetyl-neuraminic acid (DNANA) competitively inhibits the neuraminidase activity of Hemagglutinin-neuraminidase (HN) from Sendai virus. The inhibition constant depends on the presence of the Fusion (F) protein, which is 30 microM in the presence of active F protein and 50 microM when the F protei...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dallocchio F,Tomasi M,Bellini T

    更新日期:1995-03-08 00:00:00