Abstract:
:The interest in adiponutrin stems from adiponutrin variant I148M, which is strongly associated to non-alcoholic fatty liver disease. Adiponutrin has to date been considered to be solely an intracellular protein, with a role in lipid metabolism in liver and adipose tissue. However, a physiologically relevant role for adiponutrin has not been found. The aim of this study was to investigate the presence of adiponutrin in human plasma, a new facet of adiponutrin research. We demonstrate that adiponutrin is present in plasma as disulfide-bond dependent multimers, estimated to circulate at a concentration of 1.25-4 nM. Experiments reveal that adiponutrin is released from HepG2 cells in the presence of oleate. The presence of adiponutrin in plasma makes it accessible for clinical investigations and use as a potential biomarker for metabolic disease.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Winberg ME,Motlagh MK,Stenkula KG,Holm C,Jones HAdoi
10.1016/j.bbrc.2014.03.078subject
Has Abstractpub_date
2014-04-18 00:00:00pages
1114-9issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(14)00521-Xjournal_volume
446pub_type
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