Abstract:
:Recent identification of intracellular proteins that bind ammodytoxin (calmodulin, 14-3-3 proteins, and R25) suggests that this snake venom presynaptically active phospholipase A(2) acts intracellularly. As these ammodytoxin acceptors are cytosolic and mitochondrial proteins, the toxin should be able to enter the cytosol of a target cell and remain stable there to interact with them. Using laser scanning confocal microscopy we show here that Alexa-labelled ammodytoxin entered the cytoplasm of the rat hippocampal neuron and subsequently also its nucleus. The transport of proteins into the nucleus proceeds via the cytosol of a cell, therefore, ammodytoxin passed the cytosol of the neuron on its way to the nucleus. Although it is not yet clear how ammodytoxin is translocated into the cytosol of the neuron, our results demonstrate that its stability in the cytosol is not in question, providing the evidence that the toxin can act in this cellular compartment.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Petrovic U,Sribar J,Paris A,Rupnik M,Krzan M,Vardjan N,Gubensek F,Zorec R,Krizaj Idoi
10.1016/j.bbrc.2004.09.144subject
Has Abstractpub_date
2004-11-19 00:00:00pages
981-5issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(04)02201-6journal_volume
324pub_type
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