Abstract:
:From a lambda UNI-ZAP XR cDNA library derived from poly(A)+RNA of human ovarian granulosa cells a cDNA clone pHG51 was isolated. Sequence analysis showed significant homology to the C-terminal region of rat ribosomal protein L8 cDNA. The 5'-end of the cDNA was completed by PCR with cloned total cDNA. Aligning of DNA sequences from PCR clones with the sequence of the pHG51 insert yielded the full-length cDNA. From the open reading frame of the cDNA an amino acid sequence for a polypeptide of 257 residues was derived, which was identical with rat ribosomal protein L8 and also possessed a high degree of identity to ribosomal proteins L8 of other species. It is therefore assumed that the characterized cDNA represents the mRNA of the human ribosomal protein L8. Southern analysis revealed that human ribosomal protein L8 is specified by multi copy genes.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Hanes J,Klaudiny J,von der Kammer H,Scheit KHdoi
10.1006/bbrc.1993.2607subject
Has Abstractpub_date
1993-12-30 00:00:00pages
1223-8issue
3eissn
0006-291Xissn
1090-2104pii
S0006291X83726070journal_volume
197pub_type
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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更新日期:2014-09-26 00:00:00
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journal_title:Biochemical and biophysical research communications
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更新日期:2020-01-01 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章,收录出版
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.2000.3255
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(83)91622-4
更新日期:1983-07-18 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2007.04.173
更新日期:2007-07-13 00:00:00
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journal_title:Biochemical and biophysical research communications
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更新日期:2020-03-26 00:00:00