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Dynamic association of topoisomerase II to the mitotic chromosomes in live cells of Aspergillus nidulans.

Abstract：

:DNA topoisomerase II (Topo II) is an essential enzyme that catalyzes topological changes of DNA and consists of a major member of mitotic chromosomes. To investigate the dynamic localization of Topo II in nuclei, we engineered the strain of Aspergillus nidulans expressing Topo II fused with green fluorescent protein (GFP). Time-lapse microscopy revealed that the distribution of Topo II-GFP in nuclei varied depending on the cell cycle. In interphase, Topo II-GFP distributed evenly in the nucleoplasm and at the onset of G2 phase became concentrated into nucleolus. During mitosis, Topo II-GFP accumulated on chromosomes, when the chromosomes condensed. In the early mitosis, the Topo II also showed a single or two brighter spots among the fluorescence of clumped chromosomes. The spots once divided into several spots and then concentrated again into a spot per nucleus in the dividing nuclei of anaphase. Along with the subsequent decondensation of chromosomes, Topo II diffused back into nucleoplasm.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kawagishi M,Akashi T,Kikuchi A

doi

10.1016/j.bbrc.2005.06.135

subject

Has Abstract

pub_date

2005-08-26 00:00:00

pages

324-32

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)01353-7

journal_volume

334

pub_type

杂志文章

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