Nuclear-factor-kappaB (NF-kappaB) and radical oxygen species play contrary roles in transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in hepatocellular carcinoma (HCC) cells.


:Nuclear-Factor-kappaB (NF-kappaBeta can counteract transforming growth factor-beta1 (TGF-beta1)-induced apoptosis in malignant hepatocytes through up-regulation of its downstream genes, such as X-linked inhibitor of apoptosis protein (XIAP). Reports have demonstrated that TGF-beta1 can induce oxidative stress, and c-Jun N-terminal Kinase1 (JNK1) is indispensable for TGF-beta1-induced apoptosis pathway, but the relationship between radical oxygen species (ROS) and the activation of JNKs is still unclear. In the present study, we found that ROS can induce JNK activation in TGF-beta1 mediated apoptosis in hepatocytes. The inhibitors of hydrogen peroxide and superoxide, which were produced by mitochondria under stress, could inhibit the phosphorylation of c-Jun in XIAP knockdown cells. In conclusion, it is the first time to show that both NF-kappaB and antioxidants can counteract TGF-beta1-induced apoptosis in hepatic cell death through JNK1 pathway.


Wang F,Kaur S,Cavin LG,Arsura M




Has Abstract


2008-12-26 00:00:00














  • Overexpression of BCL2 blocks TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in human lung cancer cells.

    abstract::The tumor necrosis factor (TNF) related apoptosis-inducing ligand (TRAIL or Apo2L) and its receptors are members of the tumor necrosis factor superfamily. TRAIL triggers apoptosis by binding to its two proapoptotic receptors DR4 and DR5, a process which is negatively regulated by binding of TRAIL to its two decoy rece...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sun SY,Yue P,Zhou JY,Wang Y,Choi Kim HR,Lotan R,Wu GS

    更新日期:2001-01-26 00:00:00

  • The gene, expression pattern and subcellular localization of chitin synthase B from the insect Ostrinia furnacalis.

    abstract::Insect midgut peritrophic membrane (PM) is a functional structure that protects insects against chemical damage and microorganism infection. The essential component in PM is chitin and its synthesis is catalyzed by Class B chitin synthase (CHSB), which plays a unique role in chitin-containing organisms and thus repres...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Qu M,Liu T,Yang J,Yang Q

    更新日期:2011-01-07 00:00:00

  • Distribution of D(5) dopamine receptor mRNA in rat ventromedial hypothalamic nucleus.

    abstract::Estrogen induces lordosis through, in part, estrogen receptor (ER)-mediated synthesis of progesterone receptors (PR) in the ventromedial nucleus (VMN). In vitro, PR is activated by the neurotransmitter dopamine through D1-like receptors (1). In vivo, lordosis is induced by dopamine, an effect mediated in part by PR an...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhou D,Apostolakis EM,O'Malley BW

    更新日期:1999-12-20 00:00:00

  • APC0576, a novel inhibitor of NF-kappaB-dependent gene activation, prevents pro-inflammatory cytokine-induced chemokine production in human endothelial cells.

    abstract::Endothelial cells participate in the inflammatory and immune reactions. Endothelial cell activation is a recurrent phenomenon linked to the pathogenesis of diverse human diseases, such as acute and chronic inflammation and cardiovascular disorders. Pro-inflammatory cytokines (e.g., IL-1, TNF) are well-known activators...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takehana K,Konishi A,Oonuki A,Noguchi M,Fujita K,Iino Y,Kobayashi T

    更新日期:2002-05-10 00:00:00

  • Inhibition of P-glycoprotein activity and chemosensitization of multidrug-resistant ovarian carcinoma 2780AD cells by hexanoylglucosylceramide.

    abstract::In the present study we show that neutral hexanoyl-(glyco)sphingolipids inhibit P-glycoprotein (Pgp) activity in human ovarian 2780AD cells. By contrast, hexanoylceramide and the gangliosides GM(3) and GM(2) had no effect on Pgp activity, whereas sphingosine had a stimulating effect. In the case of hexanoylglucosylcer...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Veldman RJ,Sietsma H,Klappe K,Hoekstra D,Kok JW

    更新日期:1999-12-20 00:00:00

  • Characterization of the binding of the RNA-binding protein AUF1 to the human AT(1) receptor mRNA.

    abstract::An important mechanism of regulation of the expression of the AT(1) receptors is the modulation of the mRNA stability. AUF1, a human RNA-binding protein, may play an important role. Since AUF1 seems to bind to AU-rich regions of the 3'-untranslated region of the mRNAs, we verified the nucleotide sequence of human AT(1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pende A,Giacchè M,Castigliola L,Contini L,Passerone G,Patrone M,Port JD,Lotti G

    更新日期:1999-12-20 00:00:00

  • Oxytocin binding by myoepithelial cell membranes from involuted mammary tissue.

    abstract::Oxytocin binding activity of myoepithelial cell membranes from mammary tissue was measured under a variety of different experimental conditions. Mammary tissue from non-lactating rats bound oxytocin with a Kd of 9.2 +/- 1.6 nM (+/- S.E.) and indicates that receptors are retained by the myoepithelial cells in a non-lac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ruberti A,Olins GM,Eakle KA,Bremel RD

    更新日期:1983-04-29 00:00:00

  • Cyclic stretch induces upregulation of endothelin-1 with keratinocytes in vitro: possible role in mechanical stress-induced hyperpigmentation.

    abstract::The aim of this study was to investigate the possible pathological relation between mechanical stress and hyperpigmentation. We did this by investigating the influence of cyclic stretch on the expression of keratinocyte- and fibroblast-derived melanogenetic paracrine cytokines in vitro. Using primary human keratinocyt...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kurita M,Okazaki M,Fujino T,Takushima A,Harii K

    更新日期:2011-05-27 00:00:00

  • NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis.

    abstract::Endostatin, a natural angiogenesis inhibitor, had been identified for years. It opened a new approach for cancer therapy. Sequence analysis revealed that endostatin is the NC1 domain (non-triple-helical domain) of collagen XVIII. In this report, the cDNA of NC1 domain of type VIII collagen (alpha 1) was cloned and exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xu R,Yao ZY,Xin L,Zhang Q,Li TP,Gan RB

    更新日期:2001-11-23 00:00:00

  • Computer studies on the stereostructure and quantum chemical properties of 6-pyruvoyl tetrahydropterin, the key intermediate of tetrahydrobiopterin biosynthesis.

    abstract::The optimized geometry of the conformation of atoms constituting the 6-pyruvoyl tetrahydropterin molecule, the labile key intermediate of tetrahydrobiopterin biosynthesis, was obtained by molecular orbital calculations within the MINDO/3 framework. The stereostructure of the molecule showing the preferred mode for bin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Katoh S,Sueoka T,Kurihara T

    更新日期:1991-04-15 00:00:00

  • Localization of alpha-inhibin-31 in rodent testis and brain by immunocytochemical procedure and Western blot analysis.

    abstract::Using an anti-serum against synthetic alpha-inhibin-31, we have studied its cellular locations in rodent testis and brain. Western blot analysis detected several molecular forms of the protein(s). A protein of 17 KDa in size was detected in testis of one month old mice. Two major bands of molecular weights 39 kDA and ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lau YF,Li CH

    更新日期:1987-05-29 00:00:00

  • The purification and properties of cancer procoagulant from murine tumors.

    abstract::The protease, cancer procoagulant, was isolated from three murine metastatic tumors and was purified to apparent homogeneity (SDS-PAGE) from Lewis lung cells by the sequence of (NH4)2SO4 precipitation, DE-53 anion-exchange chromatography, and Sephacryl 200 chromatography. The murine tumor enzyme has a molecular weight...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Moore WR

    更新日期:1992-04-30 00:00:00

  • MNT inhibits the migration of human hepatocellular carcinoma SMMC7721 cells.

    abstract::Max binding protein (MNT) is a member of the Myc/Max/Mad network that plays a role in cell proliferation, differentiation and apoptosis. We previously observed that MNT was differentially expressed in hepatocellular carcinoma (HCC) and interacted with Nck1 by 2-DE. Nck family adaptor proteins function to couple tyrosi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu J,Zhou Q,Wang Y,Zhou X,Li J

    更新日期:2012-02-03 00:00:00

  • The 26S proteasome assembly is regulated by a maturation-inducing hormone in starfish oocytes.

    abstract::Changes in proteasome activities were observed during starfish oocyte maturation induced by a maturation-inducing hormone, 1-methyladenine. Succinyl-Leu-Leu-Val-Tyr-MCA-hydrolyzing proteasome activity in immature oocytes showed a main peak of a 1500-kDa fraction and a shoulder centered at a 650-kDa fraction on Superos...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sawada MT,Morinaga C,Izumi K,Sawada H

    更新日期:1999-01-19 00:00:00

  • Eupatilin, an activator of PPARα, inhibits the development of oxazolone-induced atopic dermatitis symptoms in Balb/c mice.

    abstract::Eupatilin (5,7-dihydroxy-3',4',6-trimethoxyflavone) is the main lipophilic flavonoid obtained from the Artemisia species. Eupatilin has been reported to have anti-apoptotic, anti-oxidative and anti-inflammatory activities. Previously, we found that eupatilin increases transcriptional activity and expression of peroxis...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jung Y,Kim JC,Park NJ,Bong SK,Lee S,Jegal H,Jin LT,Kim SM,Kim YK,Kim SN

    更新日期:2018-02-05 00:00:00

  • Cigarette smoking stimulates lipoxygenase but not cyclooxygenase pathway in platelets.

    abstract::Male rats were exposed to freshly generated cigarette smoke once daily for 4 to 8 weeks. Inhalation of smoke was verified by elevated level of carboxyhemoglobin. Arachidonate metabolism through lipoxygenase and cyclooxygenase pathways in platelets was determined. Cigarette smoking increased 12-lipoxygenase activity si...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chang WC,Fukuda S,Tai HH

    更新日期:1983-09-15 00:00:00

  • Deubiquitinating enzymes: their diversity and emerging roles.

    abstract::A growing number of important regulatory proteins within cells are modified by conjugation of ubiquitin, a well-conserved 76-amino-acid polypeptide. The ubiquitinated proteins are targeted to proteasome for degradation or alternative metabolic fates, such as triggering of plasma membrane endocytosis and trafficking to...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Chung CH,Baek SH

    更新日期:1999-12-29 00:00:00

  • Exogastrula formation in Xenopus laevis embryos depleted with maternal XmN-cadherin mRNA by antisense S-oligo DNA.

    abstract::Xenopus XmN-cadherin gene appears to have dual functions, since its mRNA is maternally provided in unfertilized eggs, once disappears almost completely during gastrula stage, then accumulates again specifically in neural tissues in later stage embryos. In the present experiment, we first followed the change in XmN-cad...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hojyo T,Tooi O,Tashiro K,Shiokawa K

    更新日期:1998-01-06 00:00:00

  • Circadian expression of clock and screening of clock-controlled genes in peripheral lymphocytes of rat.

    abstract::In this paper, the circadian pattern of Clock and genes mediated by the Clock was investigated in peripheral lymphocytes of rats. Circadian rhythms of Clock are found under the regimes of constant darkness (DD) and 12-h light-12-h dark (LD12:12h), with the peak phase at CT7 and ZT21, respectively. Ten differential cDN...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Du YZ,Fan SJ,Meng QH,Wang GQ,Tong J

    更新日期:2005-11-04 00:00:00

  • Interaction of casein kinase II with ribosomal protein L22 of Drosophila melanogaster.

    abstract::The ubiquitous eukaryotic protein kinase CKII (casein kinase II) has been found to interact with a number of cellular proteins, either through the catalytic subunit or the regulatory subunit. Using the yeast two-hybrid screening method, we found that the catalytic subunit of Drosophila melanogaster CKII (DmCKII) inter...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhao W,Bidwai AP,Glover CV

    更新日期:2002-10-18 00:00:00

  • Genetic evidence for involvement of two distinct nonhomologous end-joining pathways in repair of topoisomerase II-mediated DNA damage.

    abstract::In vertebrate cells, DNA double-strand breaks are efficiently repaired by homologous recombination or nonhomologous end-joining (NHEJ). The latter pathway relies on Ku (the Ku70/Ku86 heterodimer), DNA-PKcs, Artemis, Xrcc4, and DNA ligase IV (Lig4). Here, we show that a human pre-B cell line nullizygous for Lig4 exhibi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Adachi N,Iiizumi S,So S,Koyama H

    更新日期:2004-06-11 00:00:00

  • Blocking antibodies specific for human albumin interfere with the hemolytic activity of the membrane attack complex of complement.

    abstract::Earlier, we showed that antibodies specific for human serum albumin are able to bind to erythrocyte-associated albumin and inhibit complement mediated hemolysis. In the present study we determine if inhibition is occurring at the membrane attack phase of complement activation or at an earlier step. We show that althou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Houle JJ,Hoffmann EM

    更新日期:1994-04-15 00:00:00

  • Prompt activation of telomerase by chemical carcinogens in rats detected with a modified TRAP assay.

    abstract::The maintenance of telomere length is crucial for survival of cells. Telomerase is an RNA-containing reverse transcriptase, which is responsible for elongation of shortened telomeres. Telomerase reactivation has been suggested to be involved in malignant progressions. To study on the involvement of telomerase activati...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Miura M,Karasaki Y,Abe T,Higashi K,Ikemura K,Gotoh S

    更新日期:1998-05-08 00:00:00

  • Age-dependent and cell-population-restricted LRRK2 expression in normal mouse spleen.

    abstract::Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson's disease (PD), but its true physiological function remains unknown. In the normal mouse, LRRK2 is expressed in kidney, spleen, and lung at much higher levels than in brain, suggesting that LRRK2 may play an important role in these organ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Maekawa T,Kubo M,Yokoyama I,Ohta E,Obata F

    更新日期:2010-02-12 00:00:00

  • Functional in vivo gene transfer into the myofibers of adult skeletal muscle.

    abstract::The postmitotic nature and longevity of skeletal muscle fibers permit stable expression of any transfected gene. Direct in vivo injection of plasmid DNA, in both adult and regenerating muscles, is a safe, inexpensive, and easy approach. Here we present an optimized electroporation protocol based on the use of spatula ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Donà M,Sandri M,Rossini K,Dell'Aica I,Podhorska-Okolow M,Carraro U

    更新日期:2003-12-26 00:00:00

  • The mechanism for apo-B mRNA editing is deamination.

    abstract::Apolipoprotein (apo-) B mRNA editing at nucleotide 6666 converts cytidine to uridine, transforming the codon for glutamine-2153 to a termination codon. To investigate this editing mechanism, [a-32P] and [5-3H] CTP were incorporated into synthetic apo-B RNA. After the substrate had been edited extensively in vitro by a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Johnson DF,Poksay KS,Innerarity TL

    更新日期:1993-09-30 00:00:00

  • Cross-linking of wheat germ RNA polymerase II with dithiobis(succinimidyl propionate).

    abstract::The subunit arrangement of wheat germ RNA polymerase II was examined using the cleavable cross-linking reagent dithiobis(succinimidyl propionate). Conditions were chosen such that the enzyme was active prior to treatment, and that most of the subunits were reactive towards the reagent. Our results indicate that the en...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Bateman E,Nicholson BH

    更新日期:1984-06-15 00:00:00

  • Upstream stimulatory factors regulate the C/EBP alpha gene during differentiation of 3T3-L1 preadipocytes.

    abstract::During adipocyte differentiation, CCAAT/enhancer-binding protein alpha (C/EBPalpha) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPalpha gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggestin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kim JW,Monila H,Pandey A,Lane MD

    更新日期:2007-03-09 00:00:00

  • Tissue- and stressor-specific differential expression of two hsc70 genes in carp.

    abstract::Two genes expressing 70 kDa heat shock proteins were identified in Cyprinus carpio. The sequence similarities and the intron-interrupted structure of the coding regions indicate that carp Hsc70-1 and Hsc70-2 belong to the Hsp70 cognate subfamily. The expressions of the two hsc70 genes were followed by semi-quantitativ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ali KS,Dorgai L,Abrahám M,Hermesz E

    更新日期:2003-08-01 00:00:00

  • Direct radioimmunoassay of atrial natriuretic factor.

    abstract::A direct radioimmunoassay of atrial natriuretic factor (ANF) has been developed. The method uses a synthetic 26 amino-acid fragment (8-33 ANF) of the native peptide. Antibodies have been prepared in rabbits immunized with the peptide coupled to thyroglobulin. The radiolabelled tracer prepared by iodination according t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Gutkowska J,Thibault G,Januszewicz P,Cantin M,Genest J

    更新日期:1984-07-31 00:00:00