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Evaluation of a self-regulated in vitro hypoxic system by using chemical reactions.

Abstract：

:In this study, we established an in vitro hypoxic system driven by a self-regulated chemical reaction that proved effective for cell culture. The hypoxic device was modified from a 1.5 L polypropylene preservation box normally employed for food storage. Pyrogallic acid, sodium hydroxide, and sodium carbonate were dissolved in water and injected into the box. Sodium dihydrogen phosphate solution was injected into the box after 15 min. We measured the concentrations of oxygen and carbon dioxide in the box to determine viability of the hypoxic system. It maintained low levels of oxygen less than 0.2% and stabilizing levels of carbon dioxide at 5% for at least 96 h. Therefore, this device sustained a stable hypoxic environment that may be applicable for cell culture and in vitro studies of hypoxia.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Han N,Wang Z,Wang G,Yu J,Chen C,Huang H,Xu R

doi

10.1016/j.bbrc.2018.04.152

subject

Has Abstract

pub_date

2018-06-07 00:00:00

pages

772-776

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)30933-1

journal_volume

500

pub_type

杂志文章

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