Abstract:
:Adenylate cyclase in homogenates of guinea pig ventricles was inhibited by the stable adenosine analogs N6-phenylisopropyladenosine (PIA) and 5(1)-N-ethylcarboxamidoadenosine (NECA). Inhibition required GTP and was enhanced by sodium ion. The maximum inhibition observed was 35.1 +/- 1.1%, the EC50 (95% confidence limits, n) for PIA and NECA were 0.20 microM (0.17-0.25 microM, 6) and 0.66 microM (0.26-1.7 microM, 4) respectively. 8-Phenyltheophylline (10 and 100 microM) and isobutylemethylxanthine (100 microM) antagonized the inhibitory effects of the adenosine analogs. These results indicate that adenosine receptors of the inhibitory type (RI or A1) are present in guinea pig myocardium and may mediate some of the cardiac responses to adenosine.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Leung E,Johnston CI,Woodcock EAdoi
10.1016/0006-291x(83)91281-0subject
Has Abstractpub_date
1983-01-14 00:00:00pages
208-15issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(83)91281-0journal_volume
110pub_type
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