Targeting carbonic anhydrase to treat diabetic retinopathy: emerging evidences and encouraging results.

abstract：:Cultured endothelial cells release a potent vasoconstrictor peptide, endothelin. Cumulative addition of synthetic endothelin to isolated rabbit aortic rings elicited a concentration-dependent increase in contractile tension which was endothelium-independent. In cultured rabbit vascular smooth muscle cells loaded with ...

journal_title：Biochemical and biophysical research communications

authors： Marsden PA,Danthuluri NR,Brenner BM,Ballermann BJ,Brock TA

更新日期：1989-01-16 00:00:00

abstract：:As studied by intravital microscopy, local challenge with the mast cell secretagogue compound 48/80 was found to increase the leukocyte rolling fraction, decrease rolling velocity and induce firm leukocyte adhesion in postcapillary venules of the rat mesentery. These effects of compound 48/80 were inhibited by a monoc...

journal_title：Biochemical and biophysical research communications

authors： Thorlacius H,Raud J,Rosengren-Beezley S,Forrest MJ,Hedqvist P,Lindbom L

更新日期：1994-09-15 00:00:00

abstract：:In a primary cell culture system of fetal rat brain, the calmodulin-dependent protein-kinase IV (CaMKIV) could be induced by the thyroid hormone T3 in a time- and concentration-dependent manner, provided the tissue was excised not later than day 15 of gestation (E15) (Krebs et al., J. Biol. Chem. 271, 11055, 1996). We...

journal_title：Biochemical and biophysical research communications

authors： Krebs J,Wilson A,Kisielow P

更新日期：1997-12-18 00:00:00

abstract：BACKGROUND:Platelet microparticles (PMPs) are closely associated with diabetic macrovascular complications. This study aimed to explore the underlying mechanisms of high glucose-induced PMPs generation. METHODS:Washed platelets, obtained from the plasma of healthy male Sprague-Dawley rats, were incubated with high glu...

journal_title：Biochemical and biophysical research communications

authors： Wang GH,Ma KL,Zhang Y,Hu ZB,Liu L,Lu J,Chen PP,Lu CC,Ruan XZ,Liu BC

更新日期：2019-02-05 00:00:00

abstract：:Interleukin-1 alpha (IL-1 alpha) and interleukin-6 (IL-6), both known to be able to open the blood-brain barrier (BBB), downregulated plasma membrane-associated tyrosine phosphatase activity in primary porcine brain endothelial cells (PBEC). In contrast, transforming growth factor beta (TGF-beta) upregulated PTP activ...

journal_title：Biochemical and biophysical research communications

authors： Gloor SM,Weber A,Adachi N,Frei K

更新日期：1997-10-29 00:00:00

abstract：:Collagen synthesis in human fibroblasts was increased by the presence of 0.1M excess NaCl in the culture medium. Type analysis of the collagen produced by the cells showed the increase in the ratio of type III collagen to the total collagen. Similar NaCl effects were observed in several cell lines derived from differe...

journal_title：Biochemical and biophysical research communications

authors： Hata R,Sunada H,Nagai Y

更新日期：1983-11-30 00:00:00

abstract：:Rat serosal mast cells were tested for their ability to generate a nitric oxide-like factor by two bioassay systems: inhibition of platelet aggregation and stimulation of mast cell guanylate cyclase. Incubation of rat serosal mast cells with human washed platelets resulted in an inhibition of thrombin-induced platelet...

journal_title：Biochemical and biophysical research communications

authors： Salvemini D,Masini E,Anggard E,Mannaioni PF,Vane J

更新日期：1990-06-15 00:00:00

abstract：:Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Recently, additional activin beta subunit genes, betaC and betaE, have been identified. To explore the role of activin E, we created transgenic mice overexpressing human activin betaE subunit. There were pronounced diffe...

journal_title：Biochemical and biophysical research communications

authors： Hashimoto O,Ushiro Y,Sekiyama K,Yamaguchi O,Yoshioka K,Mutoh K,Hasegawa Y

更新日期：2006-03-10 00:00:00

abstract：:Two cDNA clones for the transacylase protein of the branched chain ketoacid dehydrogenase complex [E.C. 1.2.4.4] have been isolated from a human fetal liver cDNA expression library in lambda gt11 using antibody selection. By selective antibody elution from nitrocellulose filters containing the fusion proteins, it was ...

journal_title：Biochemical and biophysical research communications

authors： Litwer S,Danner DJ

更新日期：1985-09-16 00:00:00

abstract：:We report the discovery, semi-purification and characterization of a novel Ca2+/calmodulin-dependent protein kinase (peak I kinase) using syntide 2 as a substrate from the rabbit heart. In the study of dependence of peak I kinase on the concentration of calmodulin, half-maximal activation was obtained at approx. 2.0 x...

journal_title：Biochemical and biophysical research communications

authors： Uemura A,Okazaki K,Takesue H,Matsubara T,Hidaka H

更新日期：1995-06-15 00:00:00

abstract：:Previous reports on the chaperone activity of alpha-crystallin to prevent protein denaturation and thermal aggregation have suggested that partially denatured proteins can bind alpha-crystallin in its central region. Likewise, beta- and gamma-crystallin can also be localized to the central cavity of alpha-crystallin p...

journal_title：Biochemical and biophysical research communications

authors： Lee JS,Samejima T,Liao JH,Wu SH,Chiou SH

更新日期：1998-03-17 00:00:00

abstract：:Changes in the intermediate filament composition of rat kidney proximal tubule cells in culture have been investigated. The data suggest that differentiated tubular epithelial cells do not express vimentin, but vimentin expression is induced when the cells begin to proliferate in culture. The cultured cells are positi...

journal_title：Biochemical and biophysical research communications

authors： Hatzinger PB,Chen Q,Dong LQ,Stevens JL

更新日期：1988-12-30 00:00:00

abstract：:Recently a rabbit cDNA (F3R) was characterized as binding and causing calcium mobilization induced by the formyl-methionine-leucine-phenylalanine peptide (fMLP). In the study reported here, cloned DNAs were isolated from rabbit genomic DNA by PCR based on the sequence of F3R. The cloned DNAs have several differences i...

journal_title：Biochemical and biophysical research communications

authors： Beckmann MP,Munger WE,Kozlosky C,VandenBos T,Price V,Lyman S,Gerard NP,Gerard C,Cerretti DP

更新日期：1991-09-16 00:00:00

abstract：:Hyperphosphorylation of extracellular signal-regulated protein kinases-1/2 (ERK1/2) is known to promote cancer cell proliferation. We therefore investigated the constitutive phosphorylation levels of ERK1/2 and the expression of its downstream targets c-Fos, c-Jun, and cyclooxygenase-2 (COX-2) in biopsied human gastri...

journal_title：Biochemical and biophysical research communications

authors： Wu WK,Sung JJ,Yu L,Li ZJ,Chu KM,Cho CH

更新日期：2008-08-22 00:00:00

abstract：:A number of bioactive peptides are involved in regulating a wide range of animal behaviors, including food consumption. Vertebrate neuropeptide Y (NPY) is a potent stimulator of appetitive behavior. Recently, Drosophila neuropeptide F (dNPF) and short NPF (sNPF), the Drosophila homologs of the vertebrate NPY, were ide...

journal_title：Biochemical and biophysical research communications

authors： Ida T,Takahashi T,Tominaga H,Sato T,Kume K,Ozaki M,Hiraguchi T,Maeda T,Shiotani H,Terajima S,Sano H,Mori K,Yoshida M,Miyazato M,Kato J,Murakami N,Kangawa K,Kojima M

更新日期：2011-07-15 00:00:00

abstract：:The internucleosomal cleavage of genomic DNA is a biochemical hallmark of apoptosis. DNase gamma, a Mg2+/Ca2+-dependent endonuclease, has been suggested to be one of the apoptotic endonucleases, but its biochemical characteristic has not been fully elucidated. Here, using recombinant DNase gamma, we showed that DNase ...

journal_title：Biochemical and biophysical research communications

authors： Mizuta R,Mizuta M,Araki S,Shiokawa D,Tanuma S,Kitamura D

更新日期：2006-06-30 00:00:00

abstract：:Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken live...

journal_title：Biochemical and biophysical research communications

authors： Li L,Yao WZ,Lange AJ,Pilkis SJ,Dong MQ,Yin Y,Xu GJ

更新日期：1995-04-26 00:00:00

abstract：:The intrinsic GTPase activity of cellular protein p21ras is strongly increased by cytosolic GTPase activating protein (GAP). The activity of this enzyme has been shown to be inhibited by arachidonic acid. We report here that this inhibition is antagonized by the presence of 1-stearoyl, 2-arachidonoyl glycerol. This ef...

journal_title：Biochemical and biophysical research communications

authors： Homayoun P,Stacey DW

更新日期：1993-08-16 00:00:00

abstract：:Human carcinoma cells cultured in serum free medium produced an enzyme present as two different isoforms of 62 and 59 kDa which was found to degrade hyaluronan and chondroitin sulfate, with optimum activity at pH 4.0 and 0.03 M NaCl. The activity was suppressed by treatment with 250 mM apigenin and 1 mM DTT. The one-d...

journal_title：Biochemical and biophysical research communications

authors： Podyma KA,Yamagata S,Sakata K,Yamagata T

更新日期：1997-12-18 00:00:00

abstract：:We have studied the functional properties of an alternately spliced form of sheep testicular FSH receptor cDNA that codes for a protein similar to a previously described active receptor but differs in the carboxy terminus in sequence and is also shorter by 25 residues. The receptor expressed in HEK 293 cells fails to ...

journal_title：Biochemical and biophysical research communications

authors： Sairam MR,Jiang LG,Yarney TA,Khan H

更新日期：1996-09-24 00:00:00

abstract：:MicroRNAs are endogenously expressed regulatory noncoding RNAs. Altered expression levels of several microRNAs have been observed in glioblastomas. Functions and direct mRNA targets for these microRNAs have been relatively well studied over the last years. According to these data, it is now evident, that impairment of...

journal_title：Biochemical and biophysical research communications

authors： Novakova J,Slaby O,Vyzula R,Michalek J

更新日期：2009-08-14 00:00:00

abstract：:Monosomy 7 and interstitial deletions in the long arm of chromosome 7 (-7/7q-) is a common nonrandom chromosomal abnormality found frequently in myeloid disorders including acute myeloid leukemia (AML), myelodysplastic syndrome (MDS), and juvenile myelomonocytic leukemia (JMML). Using a short probe-based microarray co...

journal_title：Biochemical and biophysical research communications

authors： Asou H,Matsui H,Ozaki Y,Nagamachi A,Nakamura M,Aki D,Inaba T

更新日期：2009-05-29 00:00:00

abstract：:Cellular prion protein, PrP(C), is essential for the development of prion diseases where it is considered to be a substrate for the formation of the disease-associated conformer, PrP(Sc). In sheep, PrP(C) is abundant in neuronal tissue and is also found at lower concentrations in a range of non-neuronal tissues, inclu...

journal_title：Biochemical and biophysical research communications

authors： Maddison BC,Whitelam GC,Gough KC

更新日期：2007-02-02 00:00:00

abstract：:The cDNA encoding the UDP glucuronosyltransferase, UGT1A3, has been cloned and expressed in cell culture. The deduced amino acid sequence of the cDNA is 90% similar in sequence to that of a previously characterized form, UGT1A4 and to that of a third form, UGT1A5 whose function in unknown. UGT1A3, when expressed in CO...

journal_title：Biochemical and biophysical research communications

authors： Mojarrabi B,Butler R,Mackenzie PI

更新日期：1996-08-23 00:00:00

abstract：:Acute promyelocytic leukemia (APL) is characterized by a specific chromosome translocation t(15;17), which results in the fusion of the promyelocytic leukemia gene (PML) and retinoic acid receptor alpha gene (RARalpha). APL can be effectively treated with the cell differentiation inducer all-trans retinoic acid (ATRA)...

journal_title：Biochemical and biophysical research communications

authors： Lee KH,Chang MY,Ahn JI,Yu DH,Jung SS,Choi JH,Noh YH,Lee YS,Ahn MJ

更新日期：2002-09-06 00:00:00

abstract：:Oxysterols are 27-carbon oxidation products of cholesterol metabolism. Oxysterols possess several biological actions, including the promotion of cell death. Here, we examined the ability of several oxysterols to induce short-term death in cancerous (human breast cancer and mouse skin melanoma cells) and non-cancerous ...

journal_title：Biochemical and biophysical research communications

authors： Levy D,Correa de Melo T,Ohira BY,Fidelis ML,Ruiz JLM,Rodrigues A,Bydlowski SP

更新日期：2018-11-10 00:00:00

abstract：:Burkitt lymphoma cells and their counterpart of normal origin contain proteins with associated tyrosine protein, kinase activity. These proteins were isolated by affinity chromatography and Fast Pressure Liquid Chromatography. Proteins with enzyme activity had an app. M. W. of 47 KDa. This protein in extracts of Burki...

journal_title：Biochemical and biophysical research communications

authors： Semmel M,Biquard JM,Pavloff N,Hanania N,Gay F

更新日期：1989-09-15 00:00:00

abstract：:With co-treatment of potassium oxonate (PO) and xanthine sodium salt (XSS), a zebrafish larva model of acute hyperuricemia has been constructed for the first time. The results show PO 200 μM + XSS 10 μM, PO 300 μM + XSS 15 μM, and PO 400 μM + XSS 20 μM can significantly increase the level of uric acid in the zebrafish...

journal_title：Biochemical and biophysical research communications

authors： Zhang Y,Li Q,Wang F,Xing C

更新日期：2019-01-08 00:00:00

abstract：:Lung cancer causes high mortality without a declining trend and non small cell lung cancer represents 85% of all pulmonary carcinomas. MicroRNAs (miRNAs) serve as fine regulators of proliferation, migration, invasion/metastasis and angiogenesis of normal and cancer cells. Using TargetScan6.2, we predicted that the ubi...

journal_title：Biochemical and biophysical research communications

authors： Savita U,Karunagaran D

更新日期：2013-05-17 00:00:00

abstract：:We report here that at least seven low Mr GTP-binding proteins (range 21.5 to 29 kDa) are associated with the membranes of zymogen granules from rat pancreas. GTP binding proteins of similar Mr but in different relative proportions were found in the cytosolic fraction. Treatment of intact granules with either trypsin ...

journal_title：Biochemical and biophysical research communications

authors： Padfield PJ,Jamieson JD

更新日期：1991-01-31 00:00:00