Abstract:
:Two cDNA clones for the transacylase protein of the branched chain ketoacid dehydrogenase complex [E.C. 1.2.4.4] have been isolated from a human fetal liver cDNA expression library in lambda gt11 using antibody selection. By selective antibody elution from nitrocellulose filters containing the fusion proteins, it was determined that these inserts represent the transacylase protein. These data support the hypothesis that this protein is synthesized in the cytosol on transcripts independent of the other proteins of the branched chain ketoacid dehydrogenase complex.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Litwer S,Danner DJdoi
10.1016/0006-291x(85)91333-6subject
Has Abstractpub_date
1985-09-16 00:00:00pages
961-7issue
2eissn
0006-291Xissn
1090-2104pii
0006-291X(85)91333-6journal_volume
131pub_type
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