Free-soluble and outer membrane vesicle-associated VacA from Helicobacter pylori: Two forms of release, a different activity.

abstract：:Interleukin-2 (IL-2) is a cytokine essential for the growth and proliferation of T-cells. The purpose of this research was to study the effects of altering the "kink" caused by Pro in an alpha-helix of the protein. The Pro-47 residue was chosen because it was originally, but mistakenly, traced to the kink of an alpha-...

journal_title：Biochemical and biophysical research communications

authors： Lee BL,Ciardelli TL

更新日期：1997-04-17 00:00:00

abstract：:MicroRNAs (miRNAs) play crucial roles in the pancreatic carcinogenesis and progression. In the present study, we found that miR-9-5p was significantly downregulated in pancreatic cancer tissues and cell lines. The expression levels of miR-9-5p were negatively correlated with tumor stage and vessel invasion. Log-rank t...

journal_title：Biochemical and biophysical research communications

authors： Wang J,Wang B,Ren H,Chen W

更新日期：2019-01-29 00:00:00

abstract：:Homocysteine thiolactone is a highly reactive homocysteine derivative that can react easily with proteins. Protein homocysteinylation has been suggested as a possible mechanism underlying the pathological consequences of impaired homocysteine metabolism. Homocysteine inhibits key steps of angiogenesis and tumor invasi...

journal_title：Biochemical and biophysical research communications

authors： Martínez-Poveda B,Chavarría T,Sánchez-Jiménez F,Quesada AR,Medina MA

更新日期：2003-11-21 00:00:00

abstract：:Poly(ADP-ribose) polymerase (ADPRP) is a nuclear enzyme that transfers ADP-ribose from NAD+ to diverse nuclear proteins. Previously, the function of ADPRP was considered to relate exclusively to its catalytic activity. However, recent experiments have shown that ADPRP is actually an abundant DNA-binding protein, and t...

journal_title：Biochemical and biophysical research communications

authors： Nobori T,Yamanaka H,Carson DA

更新日期：1989-09-15 00:00:00

abstract：:It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-...

journal_title：Biochemical and biophysical research communications

authors： Kajiya M,Sato K,Silva MJ,Ouhara K,Do PM,Shanmugam KT,Kawai T

更新日期：2009-08-21 00:00:00

abstract：:Currently, a broad diversity of fluorescent proteins among marine organisms range from cyano-red emissions. Fluorescent proteins differ in their DNA sequences from green fluorescent protein (GFP). We identified cDNA encoding the gene of a new protein from the reef coral Hydnophora rigida of the Merulinidae family. Bot...

journal_title：Biochemical and biophysical research communications

authors： Bokhari H,Smith C,Veerendra K,Sivaraman J,Sikaroodi M,Gillevet P

更新日期：2010-06-04 00:00:00

abstract：:The peroxisome proliferator-activated receptor gamma, co-activator 1 alpha(PGC1α) effectively induced the biosynthesis of the mitochondria and the energy metabolism, and also regulated the muscle fiber-type shift. Overexpression of PGC1α gene in mice led to higher oxidative muscle fiber composition in muscle. However,...

journal_title：Biochemical and biophysical research communications

authors： Ying F,Zhang L,Bu G,Xiong Y,Zuo B

更新日期：2016-11-25 00:00:00

abstract：:MOTS-c, as a mitochondria derived peptide, exerts benefits for insulin resistance in HFD mice and against various stresses in an AMPK dependent way. Here, in the D-galactose chronic injection models, exogenous MOTS-c was given to determine its direct anti-aging effects. The body weight, insulin sensitivity and blood g...

journal_title：Biochemical and biophysical research communications

authors： Li Q,Lu H,Hu G,Ye Z,Zhai D,Yan Z,Wang L,Xiang A,Lu Z

更新日期：2019-05-28 00:00:00

abstract：:Platelet-derived growth factor-D (PDGF-D) is a more recent recognized growth factor involved in the regulation of several cellular processes, including cell proliferation, transformation, invasion, and angiogenesis by binding to and activating its cognate receptor PDGFR-β. After bile duct ligation or in the carbon tet...

journal_title：Biochemical and biophysical research communications

authors： Borkham-Kamphorst E,Alexi P,Tihaa L,Haas U,Weiskirchen R

更新日期：2015-02-13 00:00:00

abstract：:A GSH-dependent dehydroascorbate reductase (EC 1.8.5.1) was purified to homogeneity from human erythrocytes. The enzyme was a monomer of 32 kDa and was purified 133-fold from a crude DEAE-Sepharose fraction with a 25% yield. The reduced protein had a pI of 5.1 as judged by isoelectric focusing. Kinetic analysis gave a...

journal_title：Biochemical and biophysical research communications

authors： Xu DP,Washburn MP,Sun GP,Wells WW

更新日期：1996-04-05 00:00:00

abstract：:SIRT1, human ortholog of yeast SIR2 protein, deacetylates histones and several other transcription factors. Recently, SIRT1 has emerged as a drug target for treating age related diseases, type II diabetes, neurodegeneration, inflammation and cancer. Here, we have optimized production of functionally active wild type f...

journal_title：Biochemical and biophysical research communications

authors： Malik R,Kashyap A,Bansal K,Sharma P,Rayasam GV,Davis JA,Bora RS,Ray A,Saini KS

更新日期：2010-01-01 00:00:00

abstract：:The non-structural protein NS2B/NS3 serine-protease complex of the dengue virus (DENV) is required for the maturation of the viral polyprotein. Dissociation of the NS2B cofactor from NS3 diminishes the enzymatic activity of the complex. In this study, we identified a small molecule inhibitor that interferes with the i...

journal_title：Biochemical and biophysical research communications

authors： Pambudi S,Kawashita N,Phanthanawiboon S,Omokoko MD,Masrinoul P,Yamashita A,Limkittikul K,Yasunaga T,Takagi T,Ikuta K,Kurosu T

更新日期：2013-10-25 00:00:00

abstract：:Gastrin heptadecapeptides (gastrins I and II which differ in the presence of sulfate on the tyrosine of the latter) have been purified and sequenced from several mammalian species including pig, dog, cat, sheep, cow, human and rat. A 34 amino acid precursor ("big" gastrin), generally accounting for only 5% of total ga...

journal_title：Biochemical and biophysical research communications

authors： Shinomura Y,Eng J,Yalow RS

更新日期：1987-02-27 00:00:00

abstract：:The application of Fast Atom Bombardment Mass Spectrometry to the measurement of small differences in molecular weight is discussed in relation to the spectra of bovine proinsulin and a genetic variant. ...

journal_title：Biochemical and biophysical research communications

authors： Cottrell JS,Frank BH

更新日期：1985-03-29 00:00:00

abstract：:Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration...

journal_title：Biochemical and biophysical research communications

authors： Baumann U,Frank R,Blöcker H

更新日期：1988-12-30 00:00:00

abstract：:Using isolated rat hepatocytes we have shown that glutathione (GSH) depletion by glutathione-S-transferase (GST)-catalyzed conjugation with 1-bromoheptane or phorone was accompanied by a significant elevation in ascorbate synthesis. Glycogenolysis was also stimulated without a significant rise in glucose synthesis. Fu...

journal_title：Biochemical and biophysical research communications

authors： Chan TS,Wilson JX,O'Brien PJ

更新日期：2004-04-23 00:00:00

abstract：:Heart fatty acid binding protein (H-FABP) is a 15kDa protein that mediates the passage of fatty acids from the plasma membrane to sites of lipid synthesis. We report here, for the first time, that H-FABP is a potent inducer of cardiac myocyte hypertrophy, stimulating an increase in cell surface area, protein synthesis...

journal_title：Biochemical and biophysical research communications

authors： Burton PB,Hogben CE,Joannou CL,Clark AG,Hsuan JJ,Totty NF,Sorensen C,Evans RW,Tynan MJ

更新日期：1994-12-30 00:00:00

abstract：:p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activi...

journal_title：Biochemical and biophysical research communications

authors： Malicet C,Hoffmeister A,Moreno S,Closa D,Dagorn JC,Vasseur S,Iovanna JL

更新日期：2006-01-06 00:00:00

abstract：:We have developed a charge-mediated fusion method to reconstitute the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) in giant unilamellar vesicles (GUV). Intracellular Ca2+ transport by SERCA controls key processes in human cells such as proliferation, signaling, and contraction. Small-molecule effectors of SERCA are...

journal_title：Biochemical and biophysical research communications

authors： Bian T,Autry JM,Casemore D,Li J,Thomas DD,He G,Xing C

更新日期：2016-12-09 00:00:00

abstract：:We have constructed an expression plasmid for regulated expression of the hepatitis B surface antigen gene in yeast using promoter of the yeast Pho5 gene. In the yeast transformants, the monomeric HBsAg (22K dalton) was estimated to constitute approximately 3% of the total proteins. On extraction, the HBsAg was found ...

journal_title：Biochemical and biophysical research communications

authors： Choo KB,Wu SM,Lee HH,Lo SC

更新日期：1985-08-30 00:00:00

abstract：:A novel membrane-soluble prodrug of the 5'-monophosphate derivative of 3TC containing a phenyl group and the methyl ester of L-alanine linked to the phosphorus through a phosphoramidate bond with the primary amino moiety (designated Cf 1109) was prepared. The 3TC prodrug proved less potent an inhibitor of HIV-1 and HI...

journal_title：Biochemical and biophysical research communications

authors： Balzarini J,Wedgwood O,Kruining J,Pelemans H,Heijtink R,De Clercq E,McGuigan C

更新日期：1996-08-14 00:00:00

abstract：:The Apical Junctional Complex (AJC) encompassing the tight junction (TJ) and adherens junction (AJ) plays a pivotal role in regulating epithelial barrier function and epithelial cell proliferative processes through signaling events that remain poorly characterized. A potential regulator of AJC protein expression is Gl...

journal_title：Biochemical and biophysical research communications

authors： Severson EA,Kwon M,Hilgarth RS,Parkos CA,Nusrat A

更新日期：2010-07-02 00:00:00

abstract：AIMS:New Zealand obese (NZO) mice exhibit a polygenic syndrome of obesity, insulin resistance, and hypercholesterolemia that resembles the human metabolic syndrome. This study was performed in order to locate genes responsible for elevated serum cholesterol and to compare their effects under a standard and high fat die...

journal_title：Biochemical and biophysical research communications

authors： Giesen K,Plum L,Kluge R,Ortlepp J,Joost HG

更新日期：2003-05-16 00:00:00

abstract：:18β-Glycyrrhetinic acid (18β-GA) obtained from the herb liquorice has various pharmacological properties including anti-inflammatory and anti-bacterial activities. However, potential biological anti-obesity activities are unclear. In this study, novel biological activities of 18β-GA in the adipogenesis of 3T3-L1 pread...

journal_title：Biochemical and biophysical research communications

authors： Moon MH,Jeong JK,Lee YJ,Seol JW,Ahn DC,Kim IS,Park SY

更新日期：2012-04-20 00:00:00

abstract：:Nephrin is an important constituent of the glomerular filtration barrier and alteration of its expression is associated with severe proteinuria. In this study we show that injection of an anti-Thy1.1 antibody in rats not only induces a mesangioproliferative glomerulonephritis associated with increased proteinuria, but...

journal_title：Biochemical and biophysical research communications

authors： Schaefer L,Ren S,Schaefer RM,Mihalik D,Babelova A,Huwiler A,Pfeilschifter J

更新日期：2004-11-05 00:00:00

abstract：:We examined the expression of dystrophin mRNA and the protein in the developing rat heart with polymerase chain reaction, immunoblotting and immunohistochemical analyses. Dystrophin mRNA was detectable on and after the 10th embryonic day, while the protein became detectable during the perinatal period. ...

journal_title：Biochemical and biophysical research communications

authors： Tanaka H,Ozawa E

更新日期：1990-10-30 00:00:00

abstract：:The cobalt(II) derivative of cucumber basic blue copper protein "plantacyanin" has been prepared. The visible absorption, circular dichroic and magnetic circular dichroic spectra of Co(II)-plantacyanin are similar to those of Co(II)-plastocyanin, indicating that the stereochemistry of Co(II) is tetrahedral and at leas...

journal_title：Biochemical and biophysical research communications

authors： Sakurai T

更新日期：1986-09-30 00:00:00

abstract：:The 3C-like protease (3CL(pro)) of severe acute respiratory syndrome coronavirus (SARS-CoV) plays key roles in viral replication and is an attractive target for anti-SARS drug discovery. In this report, a fluorescence resonance energy transfer (FRET)-based method was developed to assess the proteolytic activity of SAR...

journal_title：Biochemical and biophysical research communications

authors： Kuang WF,Chow LP,Wu MH,Hwang LH

更新日期：2005-06-17 00:00:00

abstract：:The peroxisome proliferator activated receptors (PPARs) alpha, beta/delta, and gamma are novel nuclear hormone receptors activated by long chain fatty acids and synthetic ligands and which regulate lipid metabolism. Recent studies have detected PPARgamma mRNA in human mammary tumor cell lines. The current study examin...

journal_title：Biochemical and biophysical research communications

authors： Gimble JM,Pighetti GM,Lerner MR,Wu X,Lightfoot SA,Brackett DJ,Darcy K,Hollingsworth AB

更新日期：1998-12-30 00:00:00

abstract：:We investigated the role of acyl-CoAs during induction and maintenance of long-term potentiation in rat brain hippocampus. Changes of acyl-CoA and free fatty acids (FFA) in hippocampus were measured during tetraethylammonium (TEA)-induced LTP. Results indicated that concentrations of acyl-CoAs and FFAs in slices were ...

journal_title：Biochemical and biophysical research communications

authors： Zhang Q,Yoshida S,Sakai K,Liu J,Fukunaga K

更新日期：2000-01-07 00:00:00