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Oxysterols selectively promote short-term apoptosis in tumor cell lines.

Abstract：

:Oxysterols are 27-carbon oxidation products of cholesterol metabolism. Oxysterols possess several biological actions, including the promotion of cell death. Here, we examined the ability of several oxysterols to induce short-term death in cancerous (human breast cancer and mouse skin melanoma cells) and non-cancerous (human endothelial cells and lung fibroblasts) cell lines. We determined cell viability, Ki67 expression, cell cycle regulation, and apoptosis after 24-h incubations with oxysterols. We found that different oxysterols had different effects on the studied parameters. Moreover, the effects depended on cell type and oxysterol concentration. Three cytotoxic oxysterols (7-ketocholesterol, cholestane-3β-5α-6β-triol, and 5α-cholestane-3β,6β-diol) inhibited the S phase and stimulated the G0/G1 or G2/M phases. These oxysterols promoted apoptosis, determined with Annexin V and propidium iodide assays. These results showed that different oxysterols have cytotoxic effects depending on the cell line. The findings suggest a potential pharmacological utility of cytotoxic oxysterols.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Levy D,Correa de Melo T,Ohira BY,Fidelis ML,Ruiz JLM,Rodrigues A,Bydlowski SP

doi

10.1016/j.bbrc.2018.10.008

subject

Has Abstract

pub_date

2018-11-10 00:00:00

pages

1043-1049

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)32144-2

journal_volume

505

pub_type

杂志文章

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