An investigation on reduction process of cucumber ascorbate oxidase.

Abstract:

:Reduction process of cucumber ascorbate oxidase with L-ascorbate was investigated in detail through absorption and electron paramagnetic resonance (EPR) spectra under anaerobic condition. One of the three type I coppers (the type I copper which is oxidized rapidly (Sakurai, T. et al. (1985) Biochem. Biophys. Res. Commun. 131, 647-652)) and a pair of type III coppers only which contribute to the absorption at 330 nm were reduced faster than other two type I and the other pair of type III coppers, respectively. The principal active site of ascorbate oxidase was confirmed to be comprised of one type I, one type II and a pair of type III coppers. Type II copper seemed to be reduced after all type I and type III coppers have been reduced.

authors

Sakurai T,Sawada S,Suzuki S,Nakahara A

doi

10.1016/0006-291x(86)90041-0

subject

Has Abstract

pub_date

1986-03-13 00:00:00

pages

644-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(86)90041-0

journal_volume

135

pub_type

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