Physiological role of the association complexes of alpha-crystallin and its substrates on the chaperone activity.

Abstract:

:Previous reports on the chaperone activity of alpha-crystallin to prevent protein denaturation and thermal aggregation have suggested that partially denatured proteins can bind alpha-crystallin in its central region. Likewise, beta- and gamma-crystallin can also be localized to the central cavity of alpha-crystallin particle in vivo, which provides indirect evidence that alpha-crystallin can function as a chaperone in the intact lens. In this study, we have further demonstrated that the binding of the substrate proteins to alpha-crystallin by short-term preincubation may mimic the in vivo conditions of crystallin association. Preheating of alpha-crystallin with its substrate proteins at 60 degrees C for 20 min resulted in the formation of stable complexes between alpha-crystallin and its substrates (8.0% of insulin or 5.3% of gamma-crystallin was involved in complex formation). Under such conditions, the chaperone activity of alpha-crystallin to inhibit dithiothreitol-, ultraviolet-, or oxidation-induced protein aggregation can be greatly enhanced. Since UV-irradiation and oxidative stress are common insults to eye lenses under normal physiological conditions, the presence of alpha/gamma and alpha/beta complex in vivo may play an important role to maintain the lens in a transparent state.

authors

Lee JS,Samejima T,Liao JH,Wu SH,Chiou SH

doi

10.1006/bbrc.1998.8272

subject

Has Abstract

pub_date

1998-03-17 00:00:00

pages

379-83

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)98272-9

journal_volume

244

pub_type

杂志文章
  • Vitamin E is a MIF Inhibitor.

    abstract::Macrophage migration inhibitory factor (MIF) is known to contribute to the pathogenesis of inflammatory hyperalgesia and neuropathic pain. Prior studies have shown that Vitamin E treatment is associated with attenuated hyperalgesia and reduced neuropathic pain in rodents. Given these observations, we investigated the ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2012.01.031

    authors: Hegde B,Vadnal P,Sanghavi J,Korde V,Kulkarni-Almeida AA,Dagia NM

    更新日期:2012-02-10 00:00:00

  • Purification of Trypanosoma cruzi surface proteins involved in adhesion to host cells.

    abstract::We have identified four surface 83 kDa proteins of pI values 6.3, 6.4, 6.5 and 6.6 in T. cruzi trypomastigotes which specifically bind to rat heart myoblasts. These proteins were purified by isoelectric focusing and anion-exchange chromatography in an FPLC system. These 83 kDa proteins inhibit the attachment of trypom...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(90)90764-e

    authors: Villalta F,Lima MF,Zhou L

    更新日期:1990-10-30 00:00:00

  • Validation of methods for CYP2C9 genotyping: frequencies of mutant alleles in a Swedish population.

    abstract::Cytochrome P450 2C9 (CYP2C9) catalysis the metabolism of important drugs such as phenytoin, S-warfarin, tolbutamide, losartan, torasemide, and nonsteroidal anti-inflammatory drugs. A functional polymorphism of the CYP2C9 gene has been described. The variant alleles include CYP2C9*2 having a point mutation in exon 3 ca...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1998.9992

    authors: Yasar U,Eliasson E,Dahl ML,Johansson I,Ingelman-Sundberg M,Sjöqvist F

    更新日期:1999-01-27 00:00:00

  • The distribution of glycosyl-phosphatidylinositol anchored proteins is differentially regulated by serum and insulin.

    abstract::Glycosyl-phosphatidylinositol (GPI) anchored proteins are surveyed in two insulin sensitive cell types by surface labeling and phospholipase C-induced release into the medium. Serum starvation selectively increases both the number and intensity of a subset of GPI-anchored proteins. After serum starvation, loss of cell...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)91533-7

    authors: Lisanti MP,Darnell JC,Chan BL,Rodriguez-Boulan E,Saltiel AR

    更新日期:1989-10-31 00:00:00

  • The APC regulator CDH1 is essential for the progression of embryonic cell cycles in Xenopus.

    abstract::The orderly progression of cell cycle depends on timely destruction of key regulators through ubiquitin-mediated proteolysis. The anaphase-promoting complex (APC) is a major component of this degradation machinery and its activation is regulated by CDC20 and CDH1. We demonstrate here that CDH1 mRNA is ubiquitously exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/S0006-291X(02)00447-3

    authors: Zhou Y,Ching YP,Ng RW,Jin DY

    更新日期:2002-05-31 00:00:00

  • Cloning and expression of a rat cytochrome P-450 11 beta-hydroxylase/aldosterone synthase (CYP11B2) cDNA variant.

    abstract::A variant cytochrome P450 11 beta-hydroxylase/aldosterone synthase cDNA (CYP11B2) clone, pALDO-4, was isolated and sequenced from a rat zona glomerulosa cDNA library. This clone contains a 1521 bp open reading frame coding for a 506 amino acid residue protein which has a 30 amino acid extension peptide which is 6 resi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.1792

    authors: Zhou M,Gomez-Sanchez CE

    更新日期:1993-07-15 00:00:00

  • Cloning of a mRNA preferentially expressed in chondrocytes by differential display-PCR from a human chondrocytic cell line that is identical with connective tissue growth factor (CTGF) mRNA.

    abstract::Chondrocyte- or chondrosarcoma cell line (HCS)-specific DNA fragments were obtained using differential display-PCR. Nucleotide sequences of 32 species derived from HCS cells were determined. One of the sequence tags (tag no. 24) corresponded to the nucleotide sequence of connective tissue growth factor (CTGF). Norther...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1997.6528

    authors: Nakanishi T,Kimura Y,Tamura T,Ichikawa H,Yamaai Y,Sugimoto T,Takigawa M

    更新日期:1997-05-08 00:00:00

  • X-ray structure and characterization of a thermostable lipase from Geobacillus thermoleovorans.

    abstract::Thermo-alkalophilic bacterium, Geobacillus thermoleovorans secrets many enzymes including a 43 kDa extracellular lipase. Significant thermostability, organic solvent stability and wide substrate preferences for hydrolysis drew our attention to solve its structure by crystallography. The structure was solved by molecul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2018.11.105

    authors: Moharana TR,Pal B,Rao NM

    更新日期:2019-01-01 00:00:00

  • Co-localization of a glucose transporter and the insulin receptor in microsomes of insulin-treated rat adipocytes.

    abstract::Microsomal vesicles prepared from rat adipocytes were immuno-adsorbed to formaldehyde-fixed Staphylococcus aureus cells (Pansorbin) coated with anti-human-erythrocyte-glucose-transporter IgG. More than 75% of the glucose transporter detected was precipitated. The glucose transporter was about 10-fold enriched by the a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92261-4

    authors: Ezaki O,Bono N,Itakura H,Kasahara M

    更新日期:1989-03-31 00:00:00

  • MNT inhibits the migration of human hepatocellular carcinoma SMMC7721 cells.

    abstract::Max binding protein (MNT) is a member of the Myc/Max/Mad network that plays a role in cell proliferation, differentiation and apoptosis. We previously observed that MNT was differentially expressed in hepatocellular carcinoma (HCC) and interacted with Nck1 by 2-DE. Nck family adaptor proteins function to couple tyrosi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2011.12.140

    authors: Wu J,Zhou Q,Wang Y,Zhou X,Li J

    更新日期:2012-02-03 00:00:00

  • Modulation of estrogen receptor and epidermal growth factor receptor mRNAs by phorbol ester in MCF 7 breast cancer cells.

    abstract::Previous studies have demonstrated an inverse relationship between estrogen receptor (ER) and epidermal growth factor receptor (EGF-R) gene expression in human breast cancer cells. This relationship was further investigated in MCF 7 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). Exposure to 10 nM TPA r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(89)92013-5

    authors: Lee CS,Koga M,Sutherland RL

    更新日期:1989-07-14 00:00:00

  • Nearest neighbour algorithm for predicting protein subcellular location by combining functional domain composition and pseudo-amino acid composition.

    abstract::In this paper, based on the approach by combining the "functional domain composition" [K.C. Chou, Y. D. Cai, J. Biol. Chem. 277 (2002) 45765] and the pseudo-amino acid composition [K.C. Chou, Proteins Struct. Funct. Genet. 43 (2001) 246; Correction Proteins Struct. Funct. Genet. 2044 (2001) 2060], the Nearest Neighbou...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/s0006-291x(03)00775-7

    authors: Cai YD,Chou KC

    更新日期:2003-05-30 00:00:00

  • IL-2-mediated upregulation of uPA and uPAR in natural killer cells.

    abstract::Urokinase plasminogen activator (uPA) and its receptor uPAR play a major role in immune cell-mediated, including natural killer (NK) cell-mediated, degradation of extracellular matrices. Herein, we investigate the effects of IL-2 on NK cell uPA and uPAR. RNA and protein analyses showed upregulation of uPA and uPAR fol...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.2002.6627

    authors: Al-Atrash G,Shetty S,Idell S,Xue Y,Kitson RP,Halady PK,Goldfarb RH

    更新日期:2002-03-22 00:00:00

  • Identification of novel single-stranded DNA binding proteins recognizing octamer motif.

    abstract::We have previously identified the region containing transcriptional enhancer in mouse immunoglobulin heavy chain (IgH) gene as an initiation region of DNA replication. The octamer sequence therein has been found to be essential for initiation of DNA replication as well as for transcription. Octamer-binding protein fam...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1593

    authors: Ogawa N,Ariga H,Iguchi-Ariga SM

    更新日期:1994-05-16 00:00:00

  • DNA-binding specificity of the Lon protease alpha-domain from Brevibacillus thermoruber WR-249.

    abstract::Lon protease has been well studied in many aspects; however, the DNA-binding specificity of Lon in prokaryotes has not been clearly identified. Here we examined the DNA-binding activity of Lon protease alpha-domains from Brevibacillus thermoruber (Bt), Bacillus subtilis (Bs), and Escherichia coli (Ec). MALDI-TOF mass ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.07.118

    authors: Lin YC,Lee HC,Wang I,Hsu CH,Liao JH,Lee AY,Chen C,Wu SH

    更新日期:2009-10-09 00:00:00

  • Aortic smooth muscle cell migration caused by platelet-derived growth factor is mediated by lipoxygenase product(s) of arachidonic acid.

    abstract::The relation between platelet-derived growth factor (PDGF)-induced smooth muscle cell migration, measured in Boyden chambers, and cellular arachidonic acid cascade was studied by using rat aortic smooth muscle cells. Partially purified PDGF stimulated cell migration significantly at a concentration of 1.33-133.0 micro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(83)91697-2

    authors: Nakao J,Ito H,Chang WC,Koshihara Y,Murota S

    更新日期:1983-05-16 00:00:00

  • Selective inhibition of human immunodeficiency virus (HIV) by 3'-azido-2', 3'-dideoxyguanosine in vitro.

    abstract::3'-Azido-2',3'-dideoxyguanosine (AzddGuo) is a potent and selective inhibitor of human immunodeficiency virus (HIV) in vitro. AzddGuo completely inhibits HIV-induced cytopathogenicity and viral antigen expression in MT-4 cells at a concentration of 5.0 microM. Its 50% effective dose for inhibiting HIV-induced cytopath...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(87)91547-6

    authors: Baba M,Pauwels R,Balzarini J,Herdewijn P,De Clercq E

    更新日期:1987-06-30 00:00:00

  • Peritoneal injection of fucoidan suppresses the increase of plasma IgE induced by OVA-sensitization.

    abstract::We previously reported that fucoidan, a dietary fiber purified from seaweed, inhibited IgE production by B cells in vitro. In this study, we examined the effect of fucoidan on IgE production in vivo. The OVA-induced increase of plasma IgE was significantly suppressed when fucoidan was intraperitoneally, but not orally...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2009.07.031

    authors: Yanase Y,Hiragun T,Uchida K,Ishii K,Oomizu S,Suzuki H,Mihara S,Iwamoto K,Matsuo H,Onishi N,Kameyoshi Y,Hide M

    更新日期:2009-09-25 00:00:00

  • Regulation of the functional interactions between archaeal eukaryote-like Cdc6/Orc1 proteins on the replication origin by two different mechanisms.

    abstract::The crenarchaeon Sulfolobus solfataricus contains three active origins of replication and three eukaryote-like Cdc6/Orc1 proteins known as SsoCdc6 proteins. It has the potential to become a powerful model system in understanding the central mechanism of the eukaryotic DNA replication. In this research, we designed a g...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2007.12.087

    authors: He ZG,Feng Y,Jiang PX,Wang J

    更新日期:2008-02-22 00:00:00

  • Pak regulates calpain-dependent degradation of E3b1.

    abstract::E3b1, a binding partner of Eps8, plays a critical role in receptor tyrosine kinase (RTK)-mediated Rac activation by facilitating the interaction of Eps8 with Sos-1 and the consequent activation of the Rac-specific guanine nucleotide exchange factor activity of Sos-1. Here we present evidence that E3b1 levels are regul...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.05.042

    authors: Chi S,Chang S,Park D

    更新日期:2004-06-25 00:00:00

  • Anthelmintic drug niclosamide enhances the sensitivity of chronic myeloid leukemia cells to dasatinib through inhibiting Erk/Mnk1/eIF4E pathway.

    abstract::Chronic myeloid leukemia (CML) responds well to BCR-ABL tyrosine kinase inhibitors (TKI), but becomes resistant to TKIs after it progresses to blast phase (BP). Here we show that niclosamide, a FDA-approved anthelmintic drug, enhances the sensitivity of BP-CML cells to dasatinib (2nd generation of BCR-ABL TKI) through...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2016.08.047

    authors: Liu Z,Li Y,Lv C,Wang L,Song H

    更新日期:2016-09-16 00:00:00

  • Effects of oxysterols on the blood-brain barrier: implications for Alzheimer's disease.

    abstract::Altered brain cholesterol homeostasis plays a key role in neurodegenerative diseases such as Alzheimer's disease (AD). For a long time, the blood-brain barrier (BBB) was basically considered as a barrier isolating the brain from circulating cholesterol, however, several lines of evidence now suggest that the BBB stric...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审

    doi:10.1016/j.bbrc.2013.11.059

    authors: Gosselet F,Saint-Pol J,Fenart L

    更新日期:2014-04-11 00:00:00

  • The impact of N-cadherin-β-catenin signaling on the analgesic effects of glial cell-derived neurotrophic factor in neuropathic pain.

    abstract::Long-term neuropathic pain can lead to anxiety, depression, and other issues, which seriously affect patients' quality of life. For this reason, it is important to find effective treatments. Studies have shown that glial cell-derived neurotrophic factor (GDNF) can relieve neuropathic pain. However, its mechanism of ac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2019.11.076

    authors: Pan Y,Jiang L,Wang C

    更新日期:2020-02-05 00:00:00

  • Differential regulation of IGF-II-induced IL-8 by extracellular signal-regulated kinase 1/2 and p38 mitogen-activated protein kinases in human keratinocytes.

    abstract::In order to study the relationship between insulin like growth factor-II (IGF-II) and interleukin-8 (IL-8) that are upregulated in psoriasis, we monitored IL-8 expression in IGF-II-treated human keratinocytes and explored the signaling pathways of IL-8 expression by IGF-II. IGF-II increased the IL-8 mRNA and protein l...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2004.03.042

    authors: Kim HJ,Byun SJ,Kim TY

    更新日期:2004-04-23 00:00:00

  • STAM-AMSH interaction facilitates the deubiquitination activity in the C-terminal AMSH.

    abstract::Signal transducing adaptor molecule (STAM) complexed with hepatocyte growth factor regulated tyrosine kinase substrate (Hrs) works on sorting of cargo proteins in multivesicular body (MVB) pathway. Associated molecule with SH3 domain of STAM (AMSH), a zinc-containing ubiquitin isopeptidase, is thought to play a role i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2006.10.068

    authors: Kim MS,Kim JA,Song HK,Jeon H

    更新日期:2006-12-22 00:00:00

  • Interaction of immobilized human plasminogen and plasmin with streptokinase.

    abstract::The complex of immobilized human plasminogen (iHPlg) and streptokinase (SK) has a lower catalytic activity in hydrolysis of plasmin-specific substrate S-2251 with a kcat/Km of 0.005 microM-1 x s-1, compared to 0.031 microM-1 x s-1 of immobilized human plasmin (iHPlm) and 0.078 microM-1 x s-1 of HPlm. The SK in the com...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1993.2029

    authors: Shi GY,Chang BI,Wu DH,Wu HL

    更新日期:1993-08-31 00:00:00

  • Oxidized-LDL induce the expression of heat shock protein 70 in human endothelial cells.

    abstract::Heat shock proteins are detectable in human atherosclerotic plaques, especially in endothelial cells. In this report we show by immunofluorescence that incubation "in vitro" with OxLDL is a stress capable of inducing the expression of heat shock protein 70 in both the EAhy-926 cell line and human umbilical vein endoth...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1006/bbrc.1994.1461

    authors: Zhu W,Roma P,Pellegatta F,Catapano AL

    更新日期:1994-04-15 00:00:00

  • Down-regulation of Homer1b/c attenuates group I metabotropic glutamate receptors dependent Ca²⁺ signaling through regulating endoplasmic reticulum Ca²⁺ release in PC12 cells.

    abstract::The molecular basis for group I metabotropic glutamate receptors (mGluR1 and 5) coupling to membrane ion channels and intracellular calcium pools is not fully understood. Homer is a family of post synaptic density proteins functionally and physically attached to target proteins at proline-rich sequences. In the presen...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2014.07.044

    authors: Lv MM,Cheng YC,Xiao ZB,Sun MY,Ren PC,Sun XD

    更新日期:2014-08-08 00:00:00

  • Expression of Nischarin negatively correlates with estrogen receptor and alters apoptosis, migration and invasion in human breast cancer.

    abstract::Nischarin, a novel integrin binding protein, has been demonstrated its negative effects on cell migration and invasion. However, the biological role of Nischarin in breast cancer has not been fully elucidated yet. Our study aimed to analyze the association between Nischarin expression and clinical features of breast c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/j.bbrc.2017.01.109

    authors: Chang C,Wei W,Han D,Meng J,Zhu F,Xiao Y,Wu G,Shi X,Zhang L

    更新日期:2017-03-11 00:00:00

  • Molecular characterization of the interleukin-8 receptor.

    abstract::Recently a rabbit cDNA (F3R) was characterized as binding and causing calcium mobilization induced by the formyl-methionine-leucine-phenylalanine peptide (fMLP). In the study reported here, cloned DNAs were isolated from rabbit genomic DNA by PCR based on the sequence of F3R. The cloned DNAs have several differences i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章

    doi:10.1016/0006-291x(91)91885-g

    authors: Beckmann MP,Munger WE,Kozlosky C,VandenBos T,Price V,Lyman S,Gerard NP,Gerard C,Cerretti DP

    更新日期:1991-09-16 00:00:00