Computer studies on the stereostructure and quantum chemical properties of 6-pyruvoyl tetrahydropterin, the key intermediate of tetrahydrobiopterin biosynthesis.

abstract：:Free fatty acids (FFAs) act as extracellular signaling molecules through binding to G-protein-coupled FFA receptors (FFARs). GPR120 and GPR40 are identified as FFARs for medium- and long-chain fatty acids. In the present study, we investigated roles of GPR120 and GPR40 in cellular functions of pancreatic cancer PANC-1...

journal_title：Biochemical and biophysical research communications

authors： Fukushima K,Yamasaki E,Ishii S,Tomimatsu A,Takahashi K,Hirane M,Fukushima N,Honoki K,Tsujiuchi T

更新日期：2015-09-25 00:00:00

abstract：:We compared the intracellular signalling pathways through Ret tyrosine kinase activated by glial cell line-derived neurotrophic factor (GDNF), multiple endocrine neoplasia (MEN) 2A, or MEN 2B mutation. Tyrosine phosphorylation of Grb2-associated binder-1 (Gab1) and activation of phosphatidylinositol 3-kinase (PI 3-kin...

journal_title：Biochemical and biophysical research communications

authors： Murakami H,Iwashita T,Asai N,Shimono Y,Iwata Y,Kawai K,Takahashi M

更新日期：1999-08-19 00:00:00

abstract：:Limited proteolysis converts the 39200 molecular weight catalytic domain of rat tyrosine hydroxylase to a monomer with a molecular weight of 37600. The purified monomer is almost fully active, with minor changes in kinetic parameters at pH 7. Mass spectral analysis and N-terminal sequencing of the proteolytically gene...

journal_title：Biochemical and biophysical research communications

authors： Lohse DL,Fitzpatrick PF

更新日期：1993-12-30 00:00:00

abstract：:The lep of Azotobacter vinelandii is an 852-base-pair open reading frame (ORF) which encodes a protein of 284 amino acid residues. The translated protein shares 75% homology with leader peptidase I isolated from Pseudomonas fluorescens and 37% homology with leader peptidase I isolated from Escherichia coli. Five highl...

journal_title：Biochemical and biophysical research communications

authors： Jock CA,Pulakat L,Lee S,Gavini N

更新日期：1997-10-20 00:00:00

abstract：:The kinetic parameters of several substrates of penicillin acylase from Streptomyces lavendulae have been determined. The enzyme hydrolyses phenoxymethyl penicillin (penicillin V) and other penicillins with aliphatic acyl-chains such as penicillin F, dihydroF, and K. The best substrate was penicillin K (octanoyl penic...

journal_title：Biochemical and biophysical research communications

authors： Torres-Guzmán R,de la Mata I,Torres-Bacete J,Arroyo M,Castillón MP,Acebal C

更新日期：2002-03-01 00:00:00

abstract：:The actin regulator VASP localizes to cell-cell junctions and has been implicated in cell-cell adhesion. VASP is recruited to sites of actin dynamics by interactions with proline rich FPPPPP motifs. Zyxin and its relative LPP use FPPPPP motifs to recruit VASP to specific cellular locations, thus directing changes in a...

journal_title：Biochemical and biophysical research communications

authors： Hansen MD,Beckerle MC

更新日期：2008-06-20 00:00:00

abstract：:We present a comprehensive comparison of the anatomical distributions of the cloned mu, delta and kappa opioid receptor mRNA in rat brain. Northern blot analysis revealed that mRNA species encoding the three receptors differed in size and were differentially localized in brain regions. In peripheral tissues analyzed, ...

journal_title：Biochemical and biophysical research communications

authors： George SR,Zastawny RL,Briones-Urbina R,Cheng R,Nguyen T,Heiber M,Kouvelas A,Chan AS,O'Dowd BF

更新日期：1994-12-15 00:00:00

abstract：:To examine the possibility that the Orf135 protein of Escherichia coli functions as a hydrolyzing enzyme for a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate), we purified the recombinant Orf135 protein and incubated it with oxidized deoxynucleotides. Of the nucleotides tested, 2-hydroxydeoxyadenosine 5'-t...

journal_title：Biochemical and biophysical research communications

authors： Kamiya H,Murata-Kamiya N,Iida E,Harashima H

更新日期：2001-11-02 00:00:00

abstract：:Inactivated vaccines are widely used for prevention of viral disease. Both humoral and cellular immune responses have been shown to play important roles in the control and clearance of virus infections. CpG motif containing oligodeoxynucleotides (ODN) have recently gained considerable interest and been used as vaccine...

journal_title：Biochemical and biophysical research communications

authors： Liang H,Xiao X,Zhang X,Hu Q,Yang Y,Cen S,Deng X,Sheng W

更新日期：2019-04-02 00:00:00

abstract：:The prion diseases are neurodegenerative disorders characterized by the conversion of the PrPc (normal cellular prion) to the PrPsc (misfolded isoform). The accumulation of PrPsc within the central nervous system (CNS) leads to neurocytotoxicity by increasing oxidative stress. In addition, many neurodegenerative disor...

journal_title：Biochemical and biophysical research communications

authors： Jeong JK,Seol JW,Moon MH,Seo JS,Lee YJ,Kim JS,Park SY

更新日期：2010-10-29 00:00:00

abstract：:Ubiquitin-like proteins Rad23 and Dsk2 have recently been shown to be capable of binding both polyubiquitin chains and the 26S proteasome. The ubiquitin-like domains (Ubls) of Rad23 and Dsk2 are indispensable for their interaction with the 26S proteasome, but the proteasome subunits capable of binding the Ubl have not...

journal_title：Biochemical and biophysical research communications

authors： Saeki Y,Sone T,Toh-e A,Yokosawa H

更新日期：2002-08-30 00:00:00

abstract：:The cardioprotective effect of SUN 1165, a novel sodium channel blocker, was investigated on ischemic myocardium. Nineteen anesthetized dogs were subjected to 2 hours coronary occlusion, and divided into 2 groups. In the control group, physiological saline was infused. In the SUN 1165 group, 2 mg/kg of SUN 1165 was in...

journal_title：Biochemical and biophysical research communications

authors： Sugiyama S,Hanaki Y,Ogawa T,Hieda N,Taki K,Ozawa T

更新日期：1988-12-15 00:00:00

abstract：:Cigarette smoke (CS)-induced emphysema is attributable to matrix metalloproteinase-12 (MMP-12) in mice, however, a relationship between CS and MMP-12 is absent in human emphysema. Here, we show that cigarette smoke condensate (CSC) induces MMP-12 gene expression in airway-like epithelia through a hydrogen peroxide (H(...

journal_title：Biochemical and biophysical research communications

authors： Lavigne MC,Eppihimer MJ

更新日期：2005-04-29 00:00:00

abstract：:Bacillus thuringiensis (Bt), considered a safe insecticide, produces insecticidal proteins named Cry during sporulation, which possess exceptional immunological properties. In this work using an immunohistochemical test we demonstrated that Cry1Ac protoxin (pCry1Ac) binds to the mucosal surface of the mouse small inte...

journal_title：Biochemical and biophysical research communications

authors： Vázquez-Padrón RI,Gonzáles-Cabrera J,García-Tovar C,Neri-Bazan L,Lopéz-Revilla R,Hernández M,Moreno-Fierro L,de la Riva GA

更新日期：2000-04-29 00:00:00

abstract：:Differential methylation of CpG sites in the promoter region of the mouse Xist gene is correlated with Xist expression and X-chromosome inactivation in the female. Using oligonucleotides encompassing the differentially methylated sites as probes in band-shift assays, we have identified a nuclear protein which binds to...

journal_title：Biochemical and biophysical research communications

authors： Huntriss J,Lorenzi R,Purewal A,Monk M

更新日期：1997-06-27 00:00:00

abstract：:Myeloid leukemia factors (MLF1 and MLF2) are proteins associated with leukemia and several other cancers. However, little is known about the regulatory mechanisms underlying the stability of these proteins. Here, we show that DDB1 and CUL4 associated factor 8 (DCAF8), which can form a functional E3 ligase complex (CRL...

journal_title：Biochemical and biophysical research communications

authors： Huang D,Liu C,Sun X,Sun X,Qu Y,Tang Y,Li G,Tong T

更新日期：2020-08-20 00:00:00

abstract：:In cultured rabbit vascular smooth muscle cells (VSMC), platelet-derived growth factor (PDGF), a potent mitogen for VSMC, induced the dose- and time-dependent formation of inositol mono-, bis- and trisphosphates (IP1, IP2 and IP3, respectively). The doses of PDGF necessary for these reactions were similar to those for...

journal_title：Biochemical and biophysical research communications

authors： Kawahara Y,Kariya K,Araki S,Fukuzaki H,Takai Y

更新日期：1988-10-31 00:00:00

abstract：:Erythroid band 4.1 enhances the binding of erythroid spectrin to phosphatidylserine vesicles under conditions of ionic strength and at protein concentrations similar to those in the red cell. The extent of enhancement depends on the concentration of band 4.1; at 2 microM 4.1, spectrin binding increases approximately 1...

journal_title：Biochemical and biophysical research communications

authors： Takeshita K,MacDonald RI,MacDonald RC

更新日期：1993-02-26 00:00:00

abstract：:We report here the clinical, genetic, and molecular characterization of three Chinese families with maternally transmitted Leber's hereditary optic neuropathy (LHON). Clinical and genetic evaluations revealed the variable severity and age-of-onset in visual impairment in these families. In the affected matrilineal rel...

journal_title：Biochemical and biophysical research communications

authors： Sun YH,Wei QP,Zhou X,Qian Y,Zhou J,Lu F,Qu J,Guan MX

更新日期：2006-08-18 00:00:00

abstract：:Tooth development is a highly organized process characterized by reciprocal interactions between epithelium and mesenchyme. However, the expression patterns and functions of molecules involved in mouse tooth development are unclear from the viewpoint of explaining human dental malformations and anomalies. Here, we sho...

journal_title：Biochemical and biophysical research communications

authors： Miyado M,Ogi H,Yamada G,Kitoh J,Jogahara T,Oda S,Sato I,Miyado K,Sunohara M

更新日期：2007-11-16 00:00:00

abstract：:Triacsin C, a product of Streptmyces sp. SK-1894, was previously reported as an inhibitor of long chain acyl-CoA synthetase. Pretreatment with triacsin C (500 nM) for 1h enhanced production of platelet-activating factor in rat neutrophils, followed by stimulation with A23187 or fMLP. Amount of lyso-PAF was also augume...

journal_title：Biochemical and biophysical research communications

authors： Hayashi M,Imai Y,Naraba H,Tomoda H,Omura S,Oh-ishi S

更新日期：1992-11-16 00:00:00

abstract：:Retinyl ester concentration is regulated by retinoic acid (RA) through an autoregulatory loop, which acts on lecithin:retinol acyltransferase (LRAT). We tested whether retinol esterification activity is downregulated in human mammary carcinoma cells and whether LRAT expression is RAR-regulated. Normal human mammary ep...

journal_title：Biochemical and biophysical research communications

authors： Andreola F,Giandomenico V,Spero R,De Luca LM

更新日期：2000-12-29 00:00:00

abstract：:The insulin receptor gene is induced 8 to 10-fold during adipocyte differentiation. Plasmids containing the promoter, exon 1 and a portion of the first intron from either the mouse or human gene are able to modulate the expression of an insulin receptor/CAT gene 3 to 7-fold during differentiation. We have shown that s...

journal_title：Biochemical and biophysical research communications

authors： McKeon C,Accili D,Chen H,Pham T,Walker GE

更新日期：1997-11-26 00:00:00

abstract：:Hydrophobic modifications of transducin (T) gamma, such as farnesyl-and carboxyl-methylation, are essential for the association of T beta gamma with the photoreceptor disc membrane, and MEKA/phosducin is known to inhibit the association. In this study, we examined the effect of MEKA on the hydrophobicity of T beta gam...

journal_title：Biochemical and biophysical research communications

authors： Tanaka H,Kuo CH,Matsuda T,Fukada Y,Hayashi F,Ding Y,Irie Y,Miki N

更新日期：1996-06-25 00:00:00

abstract：:We previously showed that nuclear factor 1-A (NF1-A) binds to the silencer elements in the glutathione transferase P (GST-P) gene, and the carboxy terminal region of NF1-A represses the transcription activity of human metallothionein IIA (hMTIIA) promoter. In this study, we identified a repression region which is divi...

journal_title：Biochemical and biophysical research communications

authors： Osada S,Ikeda T,Xu M,Nishihara T,Imagawa M

更新日期：1997-09-29 00:00:00

abstract：:We describe a new approach to DNA hybridization assays using metal-enhanced fluorescence. Thiolated oligonucleotides were bound to silver particles on a glass substrate. Addition of a complementary fluorescein-labeled oligonucleotide resulted in a dramatic time-dependent 12-fold increase in fluorescence intensity duri...

journal_title：Biochemical and biophysical research communications

authors： Malicka J,Gryczynski I,Lakowicz JR

更新日期：2003-06-20 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the synergistic effect of paclitaxel (PTX) and verapamil (VERA) on adriamycin (ADR)-resistant breast cancer (MCF-7/ADR) cells. METHODS:ATP-PCA was applied to determine the inhibitory effects of PTX combined with VERA on MCF-7/ADR cells. Edu, CCK-8 and Flow cytometry ...

journal_title：Biochemical and biophysical research communications

authors： Li P,Zhong D,Gong PY

更新日期：2019-08-13 00:00:00

abstract：:RBM39, also known as splicing factor HCC1.4, acts as a transcriptional coactivator for the steroid nuclear receptors JUN/AP-1, ESR1/ER-α and ESR2/ER-β. RBM39 is involved in the regulation of the transcriptional responses of these steroid nuclear receptors and promotes transcriptional initiation. In this paper, we repo...

journal_title：Biochemical and biophysical research communications

authors： Mai S,Qu X,Li P,Ma Q,Liu X,Cao C

更新日期：2016-04-22 00:00:00

abstract：:Endothelin (ET)-1 and big ET-1 both caused contraction of isolated porcine coronary arteries, but the potency of big ET-1 was 1/100-1/200 that of ET-1. These responses were independent of the vascular endothelium. Phosphoramidon blocked the vasoconstriction caused by 30 nM big ET-1, but was ineffective on the action o...

journal_title：Biochemical and biophysical research communications

authors： Fukuroda T,Noguchi K,Tsuchida S,Nishikibe M,Ikemoto F,Okada K,Yano M

更新日期：1990-10-30 00:00:00

abstract：:The molecular mechanisms of multiple myeloma are not well defined. EEN is an endocytosis-regulating molecule. Here we report that EEN regulates the proliferation and survival of multiple myeloma cells, by regulating IGF-1 secretion. In the present study, we observed that EEN expression paralleled with cell proliferati...

journal_title：Biochemical and biophysical research communications

authors： Huang EW,Xue SJ,Li XY,Xu SW,Cheng JD,Zheng JX,Shi H,Lv GL,Li ZG,Li Y,Liu CH,Chen XH,Liu H,Li J,Liu C

更新日期：2014-05-02 00:00:00