Follitropin signal transduction: alternative splicing of the FSH receptor gene produces a dominant negative form of receptor which inhibits hormone action.

abstract：:A stability map of local secondary structure of the mRNA of the triple-helical alpha 2(I) chain domain of chicken type I collagen was obtained by plotting the free energy of the optimal secondary structure of a local segment in mRNA against the segment position along a base sequence of the mRNA. It was found that the ...

journal_title：Biochemical and biophysical research communications

authors： Zama M

更新日期：1990-03-16 00:00:00

abstract：:Recent advances in cancer biology reveal the importance of metabolic changes in cancer; however, less is known about how metabolic pathways in tumors are regulated in vivo. Here, we report analysis of the lung cancer metabolism based on different surgical procedures, namely lobectomy and partial resection. In lobectom...

journal_title：Biochemical and biophysical research communications

authors： Kikuchi N,Soga T,Nomura M,Sato T,Sakamoto Y,Tanaka R,Abe J,Morita M,Shima H,Okada Y,Tanuma N

更新日期：2020-09-10 00:00:00

abstract：:Administration of streptozotocin (STZ) induces destruction of β-cells and is widely used as an experimental animal model of type I diabetes. In neonatal rat, after low-doses of STZ-mediated destruction of β-cells, β-cells regeneration occurs and reversal of hyperglycemia was observed. However, in neonatal mice, β-cell...

journal_title：Biochemical and biophysical research communications

authors： Kataoka M,Kawamuro Y,Shiraki N,Miki R,Sakano D,Yoshida T,Yasukawa T,Kume K,Kume S

更新日期：2013-01-18 00:00:00

abstract：:A newly identified human peptide, adrenomedullin (AM) increased cAMP levels with dose and time dependencies in a manner similar to that of human calcitonin gene-related peptide (CGRP) in rat vascular smooth muscle cells (VSMC). The EC50 value of human AM is 2 x 10(-8) M which is slightly higher than that of CGRP (8.5 ...

journal_title：Biochemical and biophysical research communications

authors： Ishizaka Y,Ishizaka Y,Tanaka M,Kitamura K,Kangawa K,Minamino N,Matsuo H,Eto T

更新日期：1994-04-15 00:00:00

abstract：:Mammalian metallothioneins (MTs) possess eight highly conserved lysine residues, including three in each of two metal binding domains. We used site-directed mutagenesis to replace these intradomain lysines in Chinese hamster ovary MT2 with glutamic acid and/or glutamine. These mutant MTs were expressed in a metal sens...

journal_title：Biochemical and biophysical research communications

authors： Cody CW,Huang PC

更新日期：1994-07-29 00:00:00

abstract：:Quercetin is one of the most prominent dietary antioxidants. During its antioxidant activity, quercetin becomes oxidized into its o-quinone/quinone methide QQ. QQ is toxic since it instantaneously reacts with thiols of, e.g., proteins. In cells, QQ will initially form an adduct with glutathione (GSH), giving GSQ. We h...

journal_title：Biochemical and biophysical research communications

authors： Boots AW,Balk JM,Bast A,Haenen GR

更新日期：2005-12-16 00:00:00

abstract：:Phosphatidylethanolamine-binding proteins (PEBPs) are found in various species and have multiple functions. In this study, we purified the swine homolog of human PEBP4 (sPEBP4) from swine seminal plasma, cloned the sPEBP4 cDNA and functionally characterized this protein. The molecular mass of the purified protein was ...

journal_title：Biochemical and biophysical research communications

authors： An LP,Maeda T,Sakaue T,Takeuchi K,Yamane T,Du PG,Ohkubo I,Ogita H

更新日期：2012-07-13 00:00:00

abstract：:Vitronectin immobilized onto polystyrene microtiter wells was demonstrated to specifically bind plasminogen in a concentration-dependent manner, yielding an estimated KD = 0.4 microM. Heparin only moderately interfered with the vitronectin-plasminogen interaction, whereas high concentrations of 6-amino-hexanoic acid i...

journal_title：Biochemical and biophysical research communications

authors： Preissner KT

更新日期：1990-05-16 00:00:00

abstract：:This report describes a procedure to generate enzymatically active, isolated HIV RNase H domain. In contrast to previously described preparations, the RNA cleavage activity of the untagged RNase H domain was surprisingly similar to that of the full-length HIV-RT protein. Signature cleavages at 18 and 9 nucleotides dow...

journal_title：Biochemical and biophysical research communications

authors： Hang JQ,Rajendran S,Yang Y,Li Y,In PW,Overton H,Parkes KE,Cammack N,Martin JA,Klumpp K

更新日期：2004-04-30 00:00:00

abstract：:Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations ...

journal_title：Biochemical and biophysical research communications

authors： Long LH,Halliwell B

更新日期：2011-03-04 00:00:00

abstract：:With the development of a culture method for stratified squamous epithelial cells, tissue-engineered epithelial cell sheets have been successfully applied as clinical cell grafts. However, the implementation of these cell sheets without the use of any animal-derived materials is highly desirable. In this study, Rho-as...

journal_title：Biochemical and biophysical research communications

authors： Aslanova A,Takagi R,Yamato M,Okano T,Yamamoto M

更新日期：2015-05-01 00:00:00

abstract：:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as...

journal_title：Biochemical and biophysical research communications

authors： Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

更新日期：2005-02-04 00:00:00

abstract：:Erythroid progenitor cells synthesize 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-hydroxyeicosatetraenoic acid (15-HETE) when stimulated by erythropoietin (Ep). Maximal stimulation of 12-HETE production occurred at one hour, whereas 15-HETE activity remained constant in response to Ep for 24 hours. Lipoxygenase-s...

journal_title：Biochemical and biophysical research communications

authors： Beckman BS,Mason-Garcia M,Nystuen L,King L,Fisher JW

更新日期：1987-08-31 00:00:00

abstract：:Two types of cDNA clones of protein phosphatase 2A beta, PP-2A beta L and PP-2A beta S, were isolated from a rat testis cDNA library. PP-2A beta L was 1.8 kb in length and corresponded to the 2.0 kb mRNA that is the major species expressed in somatic tissues. PP-2A beta S was 1.3 kb in length and corresponded to the 1...

journal_title：Biochemical and biophysical research communications

authors： Sasaki K,Kitagawa Y,Shima H,Irino S,Sugimura T,Nagao M

更新日期：1990-07-16 00:00:00

abstract：:We selected the quinolone-resistant mutants from the protein F deficient Pseudomonas aeruginosa. The mutants showed cross-resistance to tetracycline, minocycline and chloramphenicol, but not to the beta-lactam antibiotics. The MIC of ofloxacin (OFLX) against the mutants, but not in the parent, became 2 to 4 times lowe...

journal_title：Biochemical and biophysical research communications

authors： Lei Y,Sato K,Nakae T

更新日期：1991-08-15 00:00:00

abstract：:COMPARE computer program suggested that benzamide riboside, BR, 3-(1-deoxy-beta-D-ribofuranosyl)benzamide, should have a similar mechanism of action as that of tiazofurin, an inhibitor of IMP dehydrogenase (IMPDH). This hypothesis was tested in K562 cells in culture. BR was cytotoxic to K562 cells with an IC50 of 2 mi...

journal_title：Biochemical and biophysical research communications

authors： Jayaram HN,Gharehbaghi K,Jayaram NH,Rieser J,Krohn K,Paull KD

更新日期：1992-08-14 00:00:00

abstract：:A rat transplantable pituitary tumor, MtT/F84, grows much faster in E2 treated rats than in normal females, but is much retarded in thyroidectomized rats. Triiodothyronine (T3) administration in a drinking water increased the tumor growth by the dose dependent manner. The tumor contained both estrogen receptor (ER) an...

journal_title：Biochemical and biophysical research communications

authors： Fujimoto N,Roy B,Watanabe H,Ito A

更新日期：1988-04-15 00:00:00

abstract：:Antisense activity in living cells has been thought to occur via a mechanism involving both DNA-mediated hybridization arrest of target mRNA and RNase H-mediated mRNA digestion. Therefore an ideal antisense agent should be permeable to the cell and possess capacities (1) to form a thermally stable duplex in vivo with ...

journal_title：Biochemical and biophysical research communications

authors： Matsukura M,Okamoto T,Miike T,Sawai H,Shinozuka K

更新日期：2002-05-24 00:00:00

abstract：:Antibodies subjected to SDS-polyacrylamide gel electrophoresis and transferred to nitrocellulose paper, have been found to retain the ability to bind specific antigen. This has been demonstrated for two groups of antibodies, directed to a) a large molecular weight glycoprotein of the human milk fat globule and b) huma...

journal_title：Biochemical and biophysical research communications

authors： Mather S,Taylor-Papadimitriou J

更新日期：1985-12-31 00:00:00

abstract：:The stability and catalytic efficacy of the L-2-haloacid dehalogenase isolated from Azotobacter sp. RC26 were studied after immobilisation on a DEAE Sephacel solid matrix. While the optimum temperature for the soluble dehalogenase falls in the range of 30-40 degrees C, the activity of the immobilised enzyme shows a fo...

journal_title：Biochemical and biophysical research communications

authors： Diez A,Prieto MI,Alvarez MJ,Bautista JM,Garrido J,Puyet A

更新日期：1996-03-27 00:00:00

abstract：:Male Djungarian hamsters (Phodopus sungorus; 45 animals per group) were either sham-exposed or exposed to a sinusoidal magnetic field for 56 days (Experiment 1: 50 Hz, 450 microTesla peak; max. dB/dt = 140 mTesla s(-1); 24 hrs day(-1)). Except for day 7, no effects were observed with respect to body weights during exp...

journal_title：Biochemical and biophysical research communications

authors： Niehaus M,Brüggemeyer H,Behre HM,Lerchl A

更新日期：1997-05-29 00:00:00

abstract：:A hammerhead ribozyme directed against murine TNFalpha (mTNFalpha) mRNA has been constructed. In vitro studies showed that this ribozyme was released from the parent molecule by flanking cis-acting hammerhead and hairpin ribozymes. This same anti-mTNFalpha ribozyme specifically cleaved both synthetically derived subst...

journal_title：Biochemical and biophysical research communications

authors： MacKay SL,Tannahill CL,Auffenberg T,Ksontini R,Copeland EM 3rd,Moldawer LL

更新日期：1999-07-05 00:00:00

abstract：:Hepatic fibrosis can be considered as wound healing process in response to hepatocellular injury. Activation of hepatic stellate cells (HSCs) is a key event of hepatic fibrosis since activated HSCs are the cellular source of enhanced extracellular matrix deposition, and reversion of liver fibrosis is accompanied by cl...

journal_title：Biochemical and biophysical research communications

authors： Valletta D,Czech B,Thasler WE,Müller M,Bosserhoff AK,Hellerbrand C

更新日期：2012-09-28 00:00:00

abstract：:By radiolabeling using NaIO4/[3H]KBH4, a new sialoglycoprotein with Mr of 19,000 was found in the secretion of rat seminal vesicle. It was shown to interact non-covalently with semen coagulum. It existed in three acidic forms with pI values of 4.1, 3.7 and 2.9 and possessed high contents of sialic acids and acidic ami...

journal_title：Biochemical and biophysical research communications

authors： Limpaseni T,Chulavatnatol M

更新日期：1986-04-29 00:00:00

abstract：:Mouse mammary carcinoma mutant cell line FUA169, characterized by high GM3 ganglioside content, was established from parent cell line FM3A/F28-7, which has high LacCer content but no GM3. Although both cell lines showed the same quantity and quality of integrin receptors, FUA169 showed much stronger adhesion to fibron...

journal_title：Biochemical and biophysical research communications

authors： Zheng M,Tsuruoka T,Tsuji T,Hakomori S

更新日期：1992-08-14 00:00:00

abstract：:On the basis of the well-known effect of ethanol poisoning on the prooxidant/antioxidant balance of human and rodent liver we tested the response of the mitochondrial manganese-containing superoxide dismutase (MnSOD) in the liver of rats following an acute ethanol load or chronically intoxicated with an alcohol-supple...

journal_title：Biochemical and biophysical research communications

authors： Koch OR,De Leo ME,Borrello S,Palombini G,Galeotti T

更新日期：1994-06-30 00:00:00

abstract：:The involvement of vitamin D in prostate carcinogenesis was investigated using the human prostatic LNCaP cells. Incubation of the LNCaP with 100 nM 1 alpha,25-dihydroxyvitamin D3 for 2 days resulted in a 30-40% suppression of cell growth, which was accompanied by a greater than 70% down-regulated expression of the pro...

journal_title：Biochemical and biophysical research communications

authors： Hsieh TY,Ng CY,Mallouh C,Tazaki H,Wu JM

更新日期：1996-06-05 00:00:00

abstract：:To evaluate the immunomodulatory effects of histamine in vivo, we analyzed an experimental syngenic tumor model using a colon adenocarcinoma cell line, CT-26, in Balb/c mice. In this model, distinct tumor growth was observed around 6 days after inoculation. Daily administration of cimetidine (0.12 mg/kg/day) significa...

journal_title：Biochemical and biophysical research communications

authors： Takahashi K,Tanaka S,Ichikawa A

更新日期：2001-03-01 00:00:00

abstract：:As an important factor leading to aging and chronic diseases, oxidative stress has become a hot research topic. Trehalose is a natural sugar widely found in many edible plants, animals and natural microorganisms, and recent studies have suggested that trehalose is an antioxidant, although its underlying molecular mech...

journal_title：Biochemical and biophysical research communications

authors： Sun L,Zhao Q,Xiao Y,Liu X,Li Y,Zhang J,Pan J,Zhang Z

更新日期：2020-01-01 00:00:00

abstract：:Lupeol, present in fruits and medicinal plants, is a biologically active compound that has been shown to have various pharmacological properties in experimental studies. In the present study, we demonstrated the modulatory effect of lupeol on 7,12-dimethylbenz[a]anthracene (DMBA)-induced alterations on cell proliferat...

journal_title：Biochemical and biophysical research communications

authors： Nigam N,Prasad S,George J,Shukla Y

更新日期：2009-04-03 00:00:00