当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Activity of the isolated HIV RNase H domain and specific inhibition by N-hydroxyimides.

Activity of the isolated HIV RNase H domain and specific inhibition by N-hydroxyimides.

Abstract：

:This report describes a procedure to generate enzymatically active, isolated HIV RNase H domain. In contrast to previously described preparations, the RNA cleavage activity of the untagged RNase H domain was surprisingly similar to that of the full-length HIV-RT protein. Signature cleavages at 18 and 9 nucleotides downstream of a recessed RNA 5'-end were retained with the isolated RNase H domain. Activity was strongly decreased by deletion of 3 amino acids from the C-terminus, consistent with an important structural or functional role of the C-terminal alpha-helix. A prototype N-hydroxyimide (2-hydroxy-4H-isoquinoline-1,3-dione) was found to inhibit the activity of the isolated HIV RNase H domain as well as the RNase H activity of full-length HIV reverse transcriptase. In contrast, the compound did not significantly inhibit the structurally closely related Escherichia coli RNase HI. Specific binding of N-hydroxyimide compounds to the isolated RNase H domain was observed by protein fluorescence quenching.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Hang JQ,Rajendran S,Yang Y,Li Y,In PW,Overton H,Parkes KE,Cammack N,Martin JA,Klumpp K

doi

10.1016/j.bbrc.2004.03.061

subject

Has Abstract

pub_date

2004-04-30 00:00:00

pages

321-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X04005492

journal_volume

317

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Critical importance of the de novo pyrimidine biosynthesis pathway for Trypanosoma cruzi growth in the mammalian host cell cytoplasm.

    abstract：:The intracellular parasitic protist Trypanosoma cruzi is the causative agent of Chagas disease in Latin America. In general, pyrimidine nucleotides are supplied by both de novo biosynthesis and salvage pathways. While epimastigotes-an insect form-possess both activities, amastigotes-an intracellular replicating form o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.073

    authors： Hashimoto M,Morales J,Fukai Y,Suzuki S,Takamiya S,Tsubouchi A,Inoue S,Inoue M,Kita K,Harada S,Tanaka A,Aoki T,Nara T

    更新日期：2012-01-20 00:00:00

  • First report of an unusual novel double mutation affecting the transcription repression domain of MeCP2 and causing a severe phenotype of Rett syndrome: Molecular analyses and computational investigation.

    abstract：:Rett syndrome is an X-linked neurodevelopmental disorder that develops a profound intellectual and motor disability and affects 1 from 10 000 to 15 000 live female births. This disease is characterized by a period of apparently normal development until 6-18 months of age when motor and communication abilities regress ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.029

    authors： Ghorbel R,Ghorbel R,Rouissi A,Fendri-Kriaa N,Ben Salah G,Belguith N,Ammar-Keskes L,Gouider-Khouja N,Fakhfakh F

    更新日期：2018-02-26 00:00:00

  • Mannoproteins from Saccharomyces cerevisiae stimulate angiogenesis by promoting the akt-eNOS signaling pathway in endothelial cells.

    abstract：:Mannoproteins (MPs) are a major component of yeast cell walls and consist of high levels of mannose in covalent complexes with proteins. MPs complexly enhance the immune system. We previously isolated a mutant yeast, K48L3, with a higher yield of MP from its cell wall than wild-type Saccharomyces cerevisiae, YPH499. W...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.09.069

    authors： Yoon BH,Lee SM,Chang HI,Ha CH

    更新日期：2019-11-19 00:00:00

  • Involvement of P2X4 receptor in P2X7 receptor-dependent cell death of mouse macrophages.

    abstract：:Interaction of P2X7 receptor with P2X4 receptor has recently been suggested, but it remains unclear whether P2X4 receptor is involved in P2X7 receptor-mediated events, such as cell death of macrophages induced by high concentrations of extracellular ATP. Here, we present evidence that P2X4 receptor does play a role in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.156

    authors： Kawano A,Tsukimoto M,Noguchi T,Hotta N,Harada H,Takenouchi T,Kitani H,Kojima S

    更新日期：2012-03-09 00:00:00

  • Characterization of a second promoter for the mouse liver/bone/kidney-type alkaline phosphatase gene: cell and tissue specific expression.

    abstract：:The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91722-o

    authors： Studer M,Terao M,Gianni M,Garattini E

    更新日期：1991-09-30 00:00:00

  • Members of the histone deacetylase superfamily differ in substrate specificity towards small synthetic substrates.

    abstract：:Histone deacetylases (HDACs) are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. Deacetylation of epsilon-acetyl-lysine residues within the N-terminal tail of core histones mediates changes in both histone-DNA and histone-non-histone protein interactions. However, surprisin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.155

    authors： Riester D,Wegener D,Hildmann C,Schwienhorst A

    更新日期：2004-11-19 00:00:00

  • Interaction of the stress protein p8 with Jab1 is required for Jab1-dependent p27 nuclear-to-cytoplasm translocation.

    abstract：:p8 is an 80 amino-acid polypeptide identified because of its remarkable over-expression in the stressed pancreas. This protein, apparently devoid of enzymatic activity, is a powerful regulator of several intracellular pathways, suggesting that it has to interact with several molecular partners to modulate their activi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.018

    authors： Malicet C,Hoffmeister A,Moreno S,Closa D,Dagorn JC,Vasseur S,Iovanna JL

    更新日期：2006-01-06 00:00:00

  • The LIM protein Ajuba is required for ciliogenesis and left-right axis determination in medaka.

    abstract：:Cilia are microtubule-based organelles that are present on the surfaces of almost all vertebrate cells. Most cilia function as sensory or molecular transport structures. Malfunctions of cilia have been implicated in several diseases of human development. The assembly of cilia is initiated by the centriole (or basal bo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.05.017

    authors： Nagai Y,Asaoka Y,Namae M,Saito K,Momose H,Mitani H,Furutani-Seiki M,Katada T,Nishina H

    更新日期：2010-06-11 00:00:00

  • Phosphorylation of human DNMT1: implication of cyclin-dependent kinases.

    abstract：:DNA methylation plays a central role in the epigenetic regulation of gene expression during development and progression of cancer diseases. The inheritance of specific DNA methylation patterns are acquired in the early embryo and are specifically maintained after cellular replication via the DNA methyltransferase 1 (D...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.04.115

    authors： Lavoie G,St-Pierre Y

    更新日期：2011-06-03 00:00:00

  • Hemoglobin M: effect of the proximal or distal histidine replacement on circular dichroism in the visible region.

    abstract：:To examine the effects of a replacement of the proximal or the distal histidine on the structure of hemoglobin (Hb), absorption and circular dichroic (CD) spectra of five species of Hbs M in the visible region were measured. Four Hbs M had a characteristic but a similar absorption spectrum upon amino acid substitution...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90101-9

    authors： Nagai M,Takama S,Yoneyama Y

    更新日期：1985-04-30 00:00:00

  • Growth and differentiation of rat hepatocytes: changes in transcription factors HNF-3, HNF-4, STAT-3, and STAT-5.

    abstract：:The liver enriched transcription factors HNF-3 and HNF-4 are known to play major roles in development and differentiation of hepatocytes. STAT-3 and STAT-5 are signaling peptides activated by a variety of cytokines and growth factors including HGF and EGF. Their role in hepatocyte growth and differentiation is yet to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9384

    authors： Runge D,Runge DM,Drenning SD,Bowen WC Jr,Grandis JR,Michalopoulos GK

    更新日期：1998-09-29 00:00:00

  • Subcellular localization of the interaction of bipolar landmarks Bud8p and Bud9p with Rax2p in Saccharomyces cerevisiae diploid cells.

    abstract：:In Saccharomyces cerevisiae, the bud site selection of diploid cells is regulated by at least four persistent landmarks, Bud8p, Bud9p, Rax1p, and Rax2p. Bud8p and Bud9p are essential for the establishment of bipolar budding and localize mainly to the distal and the proximal poles, respectively. Their subcellular local...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.102

    authors： Kato Y,Kawasaki H,Arakawa N,Hirano H

    更新日期：2010-09-03 00:00:00

  • Reaction of optically active S- and R-forms of dolichyl phosphates with activated sugars.

    abstract：:Chemical synthesis was used to produce optically active isomers of dolichol (S- and R-forms) with 18 and 19 isoprene residues. The phosphorylated polyprene was studied in rat liver microsomal GDP-mannosyl and UDP-N-acetylglucosaminyl transferase systems. The two dolichol-P forms in both transferase systems gave Vmax v...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90439-5

    authors： Löw P,Peterson E,Mizuno M,Takigawa T,Chojnacki T,Dallner G

    更新日期：1985-07-16 00:00:00

  • An aprotinin binding site localized in the hormone binding domain of the estrogen receptor from calf uterus.

    abstract：:It has been proposed that the estrogen receptor bears proteolytic activity responsible for its own transformation. This activity was inhibited by aprotinin. Incubation of transformed ER with aprotinin modified the proteolytic digestion of the hormone binding subunit by proteinase K. The smallest hormone-binding fragme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92180-8

    authors： Nigro V,Medici N,Abbondanza C,Minucci S,Moncharmont B,Molinari AM,Puca GA

    更新日期：1990-07-31 00:00:00

  • Evidence against GRB10 as the gene responsible for Silver-Russell syndrome.

    abstract：:Recent evidence shows that Silver-Russell syndrome (SRS), the major functional deficit of which is limited growth, both intrauterine and postnatal, is due to a double dose of a gene within 7p11.2-p13 that is normally expressed exclusively from the maternal copy. Of the several growth-related genes in this chromosomal ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5500

    authors： McCann JA,Zheng H,Islam A,Goodyer CG,Polychronakos C

    更新日期：2001-09-07 00:00:00

  • Inhibitory crosstalk between ERK and AMPK in the growth and proliferation of cardiac fibroblasts.

    abstract：:Extracellular signal-regulated kinase (ERK) is one of the key protein kinases that regulate the growth and proliferation in cardiac fibroblasts (CFs). As an energy sensor of cellular metabolism, AMP-activated protein kinase (AMPK) is found recently to be involved in myocardial remodeling. In this study, we investigate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.01.099

    authors： Du J,Guan T,Zhang H,Xia Y,Liu F,Zhang Y

    更新日期：2008-04-04 00:00:00

  • Rho protein inhibition blocks protein kinase C translocation and activation.

    abstract：:Small GTP-binding proteins of the Ras and Rho family participate in various important signalling pathways. Large clostridial cytotoxins inactivate GTPases by UDP-glucosylation. Using Clostridium difficile toxin B-10463 (TcdB) for inactivation of Rho proteins (RhoA/Rac/Cdc42) and Clostridium sordellii lethal toxin-1522...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8525

    authors： Hippenstiel S,Kratz T,Krüll M,Seybold J,von Eichel-Streiber C,Suttorp N

    更新日期：1998-04-28 00:00:00

  • p38 MAPK inhibition is critically involved in VEGFR-2-mediated endothelial cell survival.

    abstract：:Vascular endothelial growth factor (VEGF) promotes vasculogenesis, arteriogenesis, and angiogenesis by stimulating proliferation, migration, and cell survival of endothelial cells. VEGF mediates its actions through activation of two receptor tyrosine kinases, VEGFR-1 and VEGFR-2. Serum starvation led to apoptosis of h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01064-7

    authors： Yilmaz A,Kliche S,Mayr-Beyrle U,Fellbrich G,Waltenberger J

    更新日期：2003-07-04 00:00:00

  • Solubilization and affinity labeling of a dihydropyridine binding site from skeletal muscle: effects of temperature and diltiazem on [3H]dihydropyridine binding to transverse tubules.

    abstract：:Effects of temperature and d-cis-diltiazem (DTZ) on [3H]nitrendipine (NTD) and [3H]nimodipine (NIM) binding to skeletal muscle t-tubular membranes were studied. A decrease in temperature from 37 degrees C to 10 degrees C decreased KD and increased Bmax slightly. DTZ increased binding by increasing Bmax under all condi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90377-2

    authors： Kirley TL,Schwartz A

    更新日期：1984-08-30 00:00:00

  • Inhibitory selectivity of canecystatin: a recombinant cysteine peptidase inhibitor from sugarcane.

    abstract：:The cDNA of a cystein peptidase inhibitor was isolated from sugarcane and expressed in Escherichia coli. The protein, named canecystatin, has previously been shown to exert antifungal activity on the filamentous fungus Trichoderma reesei. Herein, the inhibitory specificity of canecystatin was further characterized. It...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.053

    authors： Oliva ML,Carmona AK,Andrade SS,Cotrin SS,Soares-Costa A,Henrique-Silva F

    更新日期：2004-08-06 00:00:00

  • The high affinity murine laminin receptor is a member of a multicopy gene family.

    abstract：:The high affinity laminin receptor is differentially expressed in metastasis. We now report that there are multiple copies (6 +/- 1) of the laminin receptor gene in the murine genome of normal diploid cells as well as in cell lines derived from cancer cells. We have analyzed three distinct cDNA clones isolated from an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81203-3

    authors： Fernández MT,Castronovo V,Rao CN,Sobel ME

    更新日期：1991-02-28 00:00:00

  • Aging-associated increase of gelsolin for apoptosis resistance.

    abstract：:Gelsolin, a Ca(2+)-dependent actin regulatory protein, was recently suggested to participate in apoptosis regulation. In this study, we found that the level of gelsolin is elevated in senescent human diploid fibroblasts (HDFs) and also in the tissues of old rats, i.e., in the liver, kidney, heart, spleen, stomach, and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.061

    authors： Ahn JS,Jang IS,Kim DI,Cho KA,Park YH,Kim K,Kwak CS,Chul Park S

    更新日期：2003-12-26 00:00:00

  • Photoaffinity labeling studies of the rat renal sodium bile salt cotransport system.

    abstract：:The uptake of a photolabile taurocholate derivative, (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-cholan-24-oyl)-2-aminoethanesulfonate, 7,7-azo-TC, into rat renal brush-border membrane vesicles was stimulated by Na+ and inhibited by taurocholate indicating an interaction with the Na+/bile salt cotransport system. Irra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90353-6

    authors： Burckhardt G,Kramer W,Kurz G,Wilson FA

    更新日期：1987-03-30 00:00:00

  • The role of alternative splicing and C-terminal amino acids in thromboxane receptor stabilization.

    abstract：:The thromboxane receptor has two alternatively spliced isoforms, alpha and beta, which differ only in sequences within the cytoplasmic C-terminal domain. Oxidative stress induced by H(2)O(2) in a COS-7 cell model results in stabilization of the thromboxane receptor beta isoform by translocation from the endoplasmic re...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.058

    authors： Valentin F,Tippins JR,Field MC

    更新日期：2005-04-15 00:00:00

  • Microinjection of an nm23 specific antibody inhibits cell division in rat embryo fibroblasts.

    abstract：:Expression of the nm23/NDP-K gene correlates with reduced metastasis in some tumors and with increased proliferation in both nontransformed and transformed cells in culture. Decreased nm23/NDP-K expression results in mitotic arrest in neuroblasts of developing Drosophila. In order to better understand the biological r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2049

    authors： Sorscher SM,Steeg P,Feramisco JR,Buckmaster C,Boss GR,Meinkoth J

    更新日期：1993-08-31 00:00:00

  • Glycosylation is a novel TGFβ1-independent post-translational modification of Smad2.

    abstract：:Smad2 is a crucial component of intracellular signaling by transforming growth factor-β (TGFβ). Here we describe that Smad2 is glycosylated, which is a novel for Smad2 post-translational modification. We showed that the Smad2 glycosylation was inhibited upon treatment of cells with 17β-estradiol, and was enhanced in c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.039

    authors： Gotoh T,Iwahana H,Kannan S,Marei RG,Mousa H,Elgamal M,Souchelnytskyi S

    更新日期：2020-01-22 00:00:00

  • Attenuation of experimental autoimmune uveoretinitis in mice by IKKβ inhibitor IMD-0354.

    abstract：:Uveitis is a sight-threatening intraocular inflammatory disease that accounts for almost 10% of blindness worldwide. NF-κB signaling plays pivotal roles in inflammatory diseases. We have reported that IMD-0354, which inhibits NF-κB signaling via selective blockade of IKK-β, suppresses inflammation in several ocular di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.117

    authors： Liu Y,Kitaichi N,Wu D,Hase K,Satoh M,Iwata D,Namba K,Kanda A,Noda K,Itai A,Iwabuchi K,Ishida S

    更新日期：2020-05-07 00:00:00

  • BDNF induction with mild exercise in the rat hippocampus.

    abstract：:Although chronic voluntary physical activity has been shown to enhance hippocampal brain-derived neurotrophic factor (BDNF) expression in animals, the effects of forced exercise on a treadmill have not been fully investigated. We assessed induction of c-fos and BDNF expression with acute exercise at different running ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.173

    authors： Soya H,Nakamura T,Deocaris CC,Kimpara A,Iimura M,Fujikawa T,Chang H,McEwen BS,Nishijima T

    更新日期：2007-07-13 00:00:00

  • Purification and molecular properties of ascorbate peroxidase from bovine eye.

    abstract：:Ascorbate peroxidase (APX) is a hydrogen peroxide-scavenging peroxidase which uses ascorbate (AsA) as the specific electron donor. APX has not been isolated in mammals. Ocular tissue contains AsA at high concentrations, and we detected APX activity in bovine retinal pigment epithelium (RPE) and choroid. We purified AP...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7946

    authors： Wada N,Kinoshita S,Matsuo M,Amako K,Miyake C,Asada K

    更新日期：1998-01-14 00:00:00

  • Affinity purification of HIV-1 and HIV-2 proteases from recombinant E. coli strains using pepstatin-agarose.

    abstract：:A procedure is described which employs pepstatin-agarose for the affinity purification of either HIV-1 or HIV-2 protease from two similar recombinant E. coli constructs that were developed for the expression of these enzymes. HIV-2 protease was routinely expressed at much higher levels than the HIV-1 enzyme and pepsta...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91356-w

    authors： Rittenhouse J,Turon MC,Helfrich RJ,Albrecht KS,Weigl D,Simmer RL,Mordini F,Erickson J,Kohlbrenner WE

    更新日期：1990-08-31 00:00:00