Abstract:
:Chemical synthesis was used to produce optically active isomers of dolichol (S- and R-forms) with 18 and 19 isoprene residues. The phosphorylated polyprene was studied in rat liver microsomal GDP-mannosyl and UDP-N-acetylglucosaminyl transferase systems. The two dolichol-P forms in both transferase systems gave Vmax values which for the S-form exceeded 4-6 times what was obtained with the R-form. The Km values were also higher for the S-form. The hepatocyte appears to contain a large excess of dolichyl-P, by 100 times exceeding that of the Km values. For this reason the S-form of dolichyl-P seems to be one of the requirements for the normal establishment of the N-glycosidically linked oligosaccharide chain.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Löw P,Peterson E,Mizuno M,Takigawa T,Chojnacki T,Dallner Gdoi
10.1016/0006-291x(85)90439-5subject
Has Abstractpub_date
1985-07-16 00:00:00pages
460-6issue
1eissn
0006-291Xissn
1090-2104pii
0006-291X(85)90439-5journal_volume
130pub_type
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