Purification and molecular properties of ascorbate peroxidase from bovine eye.

abstract：:The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells ...

journal_title：Biochemical and biophysical research communications

authors： Warncke M,Vogt B,Ulrich J,von Laer MD,Beyer W,Klump H,Micheel B,Sheriff A

更新日期：2004-06-04 00:00:00

abstract：:PKCdelta was revealed to make a homologous protein complex that shows a high protein kinase activity upon H(2)O(2) stimulation by expressing the enzymes having different epitope tags in COS-7 cells. The association of the endogenous PKCdelta in the cells was observed by sucrose density gradients. Analysis using the mu...

journal_title：Biochemical and biophysical research communications

authors： Umada-Kajimoto S,Yamamoto T,Matsuzaki H,Kikkawa U

更新日期：2006-03-03 00:00:00

abstract：:Two Chinese hamster ovary cell clones resistant to okadaic acid (OA) were isolated. The OA-resistance was associated with resistance to colchicine, Vinca alkaloids and inhibitors of DNA topoisomerase (topo) II. Drug accumulation assays showed that the intracellular levels of OA, vinblastine and vincristine, but not th...

journal_title：Biochemical and biophysical research communications

authors： Tohda H,Yasui A,Yasumoto T,Nakayasu M,Shima H,Nagao M,Sugimura T

更新日期：1994-09-15 00:00:00

abstract：:We have examined the role of K+ channels in mediating vasorelaxation produced by bacterial lipopolysaccharide (LPS) in endothelial-denuded strips of rat aorta precontracted with phenylephrine (1 microM). Salmonella typhosa LPS (0.1 microgram/ml) caused significant relaxation of tension which peaked at approximately 4h...

journal_title：Biochemical and biophysical research communications

authors： Hall S,Turcato S,Clapp L

更新日期：1996-07-05 00:00:00

abstract：:Epimorphin, a mesenchymal cell surface-associated molecule, is detected on hepatic stellate cells (HSCs) in the liver. Here, we show the involvement of epimorphin in differentiation of rat hepatic stem-like cells (HSLCs) through contact with HSCs. HSLCs, isolated from adult rats, cultured in stellate cell-conditioned ...

journal_title：Biochemical and biophysical research communications

authors： Miura K,Nagai H,Ueno Y,Goto T,Mikami K,Nakane K,Yoneyama K,Watanabe D,Terada K,Sugiyama T,Imai K,Senoo H,Watanabe S

更新日期：2003-11-14 00:00:00

abstract：:Membrane rafts are small and dynamic regions enriched in sphingolipids, cholesterol, ganglioside GM1 and protein markers like flotillins, forming the flatter domains or caveolins, which are characterized as stable flask-shape invaginations. We explored whether membrane rafts participate in the entry of Trypanosoma cru...

journal_title：Biochemical and biophysical research communications

authors： Barrias ES,Dutra JM,De Souza W,Carvalho TM

更新日期：2007-11-23 00:00:00

abstract：:A gene encoding the human immunodeficiency virus-1 (HIV-1) TAT protein was chemically synthesized and expressed in HeLa cells and in a cell-free system. To facilitate both the assembly of the synthetic gene and further mutagenesis and gene fusion studies, several unique restriction endonuclease cleavage sites were inc...

journal_title：Biochemical and biophysical research communications

authors： Ferguson BQ,Friedland BK,Hendrickson E,Strehl L,Petteway SR,Jonak GJ

更新日期：1989-05-15 00:00:00

abstract：:Fura-2 loaded pancreatic beta-cells, isolated from obese hyperglycemic mice, were studied with respect to cytoplasmic free Ca2+ concentration ([Ca2+]i), insulin release and efflux of indicator. In the absence of glucose there was a continuous efflux of fura-2, which was markedly increased by stimulation with a high co...

journal_title：Biochemical and biophysical research communications

authors： Arkhammar P,Nilsson T,Berggren PO

更新日期：1989-02-28 00:00:00

abstract：:A novel N-terminally substituted Pro(3) analogue of glucose-dependent insulinotropic polypeptide (GIP) was synthesized and tested for plasma stability and biological activity both in vitro and in vivo. Native GIP was rapidly degraded by human plasma with only 39 +/- 6% remaining intact after 8 h, whereas (Pro(3))GIP w...

journal_title：Biochemical and biophysical research communications

authors： Gault VA,O'Harte FP,Harriott P,Flatt PR

更新日期：2002-02-08 00:00:00

abstract：:GLP-1 can help to overcome problems of liver cells metabolism, not only pancreatic cell. But the explicit mechanism of this effect remains unclear. In recent years, microRNAs have received the attention of researchers and some microRNAs have important implications for diabetes. The mitochondrial protective gene PGC-1α...

journal_title：Biochemical and biophysical research communications

authors： Wang C,Li Q,Wang W,Guo L,Guo C,Sun Y,Zhang J

更新日期：2015-10-09 00:00:00

abstract：:The PAX3 transcription factor is the key regulator of melanocyte development during embryogenesis and is also frequently found in melanoma cells. While PAX3 is known to regulate melanocyte differentiation, survival, proliferation and migration during development, it is not clear if its function is maintained in adult ...

journal_title：Biochemical and biophysical research communications

authors： Medic S,Rizos H,Ziman M

更新日期：2011-08-12 00:00:00

abstract：:MicroRNAs (miRNAs) have been shown to be involved in the pathophysiological processes of pain. At present, the roles and mechanisms of miRNAs in neonatal repetitive pain are largely unknown. In our research, the expression of miR-140-3p was increased in premature infants who received repetitive painful stimuli since a...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Yin J,Chen X,Mao X,Xu J,Cheng R,Wu J

更新日期：2019-08-06 00:00:00

abstract：:The importance of circular RNAs (circRNAs) in human cancers has gradually been acknowledged. In hepatocellular carcinoma (HCC), several circRNAs have been reported to regulate tumor growth and metastasis. However, the role of hsa_circ_0000673 in HCC remains largely unknown. In this study, we found that hsa_circ_000067...

journal_title：Biochemical and biophysical research communications

authors： Jiang W,Wen D,Gong L,Wang Y,Liu Z,Yin F

更新日期：2018-06-02 00:00:00

abstract：:Isoprenoids comprise a diverse group of natural products with a broad range of metabolic functions. Isoprenoids are synthesized from prenyl pyrophosphates by prenyltransferases that catalyze the isoprenoid chain-elongation process to different chain lengths. We hereby present the crystal structure of geranylgeranyl py...

journal_title：Biochemical and biophysical research communications

authors： Kim S,Kim EJ,Park JB,Kim SW,Kim KJ

更新日期：2019-10-20 00:00:00

abstract：:In cancer treatment, specifically targeting cancer cells is important for optimal therapeutic efficacy. One strategy is to utilize a cancer specific promoter to express a cytotoxic gene or a viral gene required for replication. In this approach, the therapeutic window is dependent on the relative promoter activity in ...

journal_title：Biochemical and biophysical research communications

authors： Kurayoshi K,Shiromoto A,Ozono E,Iwanaga R,Bradford AP,Araki K,Ohtani K

更新日期：2017-01-29 00:00:00

abstract：:The existence of cancer stem cells within the tumor could lead to cancer therapy resistance. TGFβ1 is considered as one of the most powerful players in the generation of CSCs through induction of epithelial-mesenchymal transition in different types of cancer including lung cancer, however, the detailed mechanisms by w...

journal_title：Biochemical and biophysical research communications

authors： Zhang F,Li T,Han L,Qin P,Wu Z,Xu B,Gao Q,Song Y

更新日期：2018-02-19 00:00:00

abstract：:We examined the possibility that bilirubin physiologically acts as an antioxidant by using scurvy-prone ODS-od/od rats treated with endotoxin (lipopolysaccharide: LPS). Recently, bilirubin oxidative metabolites were isolated from human urine and named biotripyrrin-a and biotripyrrin-b. The LPS injection markedly incre...

journal_title：Biochemical and biophysical research communications

authors： Yamaguchi T,Horio F,Hashizume T,Tanaka M,Ikeda S,Kakinuma A,Nakajima H

更新日期：1995-09-05 00:00:00

abstract：:A guinea pig adrenal hydroxysteroid sulfotransferase (gpHST2) has been cloned that is distinct from guinea pig hydroxysteroid sulfotransferase that stereoselectively acts on 3 alpha-hydroxylated neutral steroids (gp3 alpha HST, redesignated gpHST1). The deduced amino acid sequences for gpHST1 and gpHST2 are 86% identi...

journal_title：Biochemical and biophysical research communications

authors： Luu NX,Driscoll WJ,Martin BM,Strott CA

更新日期：1995-12-26 00:00:00

abstract：:Studies have shown similarities between the histopathological characteristics of NPC and Alzheimer's disease (AD) including amyloid and tau pathologies. While dysfunction in insulin signaling was widely detected in AD brain, the function of insulin signaling proteins has not been examined in NPC disease. In this study...

journal_title：Biochemical and biophysical research communications

authors： Ong QR,Lim ML,Chua CC,Cheung NS,Wong BS

更新日期：2012-08-03 00:00:00

abstract：:Protein phosphatase 2Cs (PP2Cs) have been referred to act as negative modulators of the protein kinase pathways involved in different environmental stress responses and developmental processes. In Arabidopsis, PP2Cs have been extensively studied and some are known to negatively regulate abscisic acid signaling. In ric...

journal_title：Biochemical and biophysical research communications

authors： Amir Hossain M,Ahn CH,Lee IS,Jeon JS,An G,Park PB

更新日期：2018-09-10 00:00:00

abstract：:The thyroid hormone receptor alpha gene is alternatively spliced to give the alpha receptor and a variant which does not bind thyroid hormones. An additional alpha-like receptor (Rev-erbA alpha) is transcribed on the opposite strand and overlaps the unique variant sequence. The alpha receptor in rat brain reaches peak...

journal_title：Biochemical and biophysical research communications

authors： Jannini EA,Mitsuhashi T,Nikodem VM

更新日期：1992-04-30 00:00:00

abstract：:To relate specific effects on growth and transformation to activation of specific affinity states of the CCKA receptor stably expressed in CHO cells we compared responses to the CCK analogues JMV-180 and CCK8. CCK8 led to an inhibition of both cell proliferation and transformation. Effects on proliferation were indica...

journal_title：Biochemical and biophysical research communications

authors： Detjen K,Tseng MJ,Logsdon CD

更新日期：1995-08-04 00:00:00

abstract：:The thiazide-sensitive Na-Cl cotransporter SLC12A3 displays expression restricted to distal convoluted tubule cells where it catalyzes the uptake of sodium and chloride through the apical membrane. We sequenced 1959 bp of the 5' flanking region of human SLC12A3, located the area of transcription initiation, and used d...

journal_title：Biochemical and biophysical research communications

authors： MacKenzie S,Vaitkevicius H,Lockette W

更新日期：2001-04-13 00:00:00

abstract：:The sequence of a novel cGMP-regulated, tetrameric, K(+) selective channel (Sp-tetraKCNG) was discovered in the sea urchin Strongylocentrotus purpuratus. The Sp-tetraKCNG is a single polypeptide made of four KCNG domains similar to voltage-dependent Na(+) and Ca(2+) channels. Each KCNG domain has six transmembrane seg...

journal_title：Biochemical and biophysical research communications

authors： Galindo BE,de la Vega-Beltrán JL,Labarca P,Vacquier VD,Darszon A

更新日期：2007-03-16 00:00:00

abstract：:2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) brings about a wide spectrum of toxic and biochemical changes, most of which are mediated by the AH receptor (AHR). Recent cloning of the AHR from the two most TCDD-resistant laboratory animals, Han/Wistar (Kuopio) rats and hamsters, suggested a critical role for the C-termin...

journal_title：Biochemical and biophysical research communications

authors： Korkalainen M,Tuomisto J,Pohjanvirta R

更新日期：2001-08-03 00:00:00

abstract：:The p53 tumor suppressor protein is a critical checkpoint in prevention of tumor formation, and the function of p53 is dependent on proper formation of the active tetramer. In vitro studies have shown that p53 binds DNA most efficiently as a tetramer, though inactive p53 is predicted to be monomeric in vivo. We demons...

journal_title：Biochemical and biophysical research communications

authors： Webber TM,Allen AC,Ma WK,Molloy RG,Kettelkamp CN,Dow CA,Gage MJ

更新日期：2009-06-19 00:00:00

abstract：OBJECTIVE:Hepatic stellate cells (HSC) activation and proliferation mediated the pathogenic development of hepatic fibrosis (HF). However, the underlying mechanisms remain poorly understood. In this study, we aimed to investigate the miR-29a-3p and its effects on PIK3R3 expression in HF pathogenesis. METHODS:LX-2 cell...

journal_title：Biochemical and biophysical research communications

authors： Fu J,Wu B,Zhong S,Deng W,Lin F

更新日期：2020-09-03 00:00:00

abstract：:Aerobic incubation of rat spermatozoa in a balanced salt solution containing 10 microM of gossypol produced a loss of sperm motility. This effect was not reverted by the presence of 25 mM Theophylline, a well-known stimulant of motility, but was significantly diminished by the addition of 10 mM Mannitol. As mannitol i...

journal_title：Biochemical and biophysical research communications

authors： Fornés MW,Barbieri AM,Burgos MH

更新日期：1993-09-30 00:00:00

abstract：:The peroxisome proliferator-activated receptor gamma, co-activator 1 alpha(PGC1α) effectively induced the biosynthesis of the mitochondria and the energy metabolism, and also regulated the muscle fiber-type shift. Overexpression of PGC1α gene in mice led to higher oxidative muscle fiber composition in muscle. However,...

journal_title：Biochemical and biophysical research communications

authors： Ying F,Zhang L,Bu G,Xiong Y,Zuo B

更新日期：2016-11-25 00:00:00

abstract：:Cancer cell molecular mimicry of stem cells (SC) follows with enhanced proliferative and renewal capacities. In support, numerous mediators of SC self-renewal have been evinced to exhibit oncogenic potential. More and more researches showed that the embryonic stem cell self-renewal genes express in various cancer cell...

journal_title：Biochemical and biophysical research communications

authors： Deng L,Xiang X,Yang F,Xiao D,Liu K,Chen Z,Zhang R,Feng G

更新日期：2017-08-19 00:00:00