当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media.

Artefacts in cell culture: α-Ketoglutarate can scavenge hydrogen peroxide generated by ascorbate and epigallocatechin gallate in cell culture media.

Abstract：

:Ascorbate and several phenolic compounds readily oxidise in cell culture media to generate hydrogen peroxide. However, addition of α-ketoglutarate, which is known to be released by several cell types, decreased the levels of H(2)O(2), and the α-ketoglutarate was depleted and converted to succinate. These observations could account for previous reports of the protective effects of α-ketoglutarate in promoting the growth of cells in culture, and may contribute to explaining some of the variability in the literature in reported rates of H(2)O(2) production from autoxidisable compounds in cell culture systems.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Long LH,Halliwell B

doi

10.1016/j.bbrc.2011.01.091

subject

Has Abstract

pub_date

2011-03-04 00:00:00

pages

20-4

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(11)00134-3

journal_volume

406

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Transcriptional activation of the human stress-inducible transcriptional repressor ATF3 gene promoter by p53.

    abstract：:Activating transcription factor 3 (ATF3) is an immediate early response gene that is induced in cells exposed to a variety of stress stimuli. In this report, upon exposure of cells to ultraviolet (UV) or proteasome inhibitor MG132, ATF3 protein was induced more efficiently in cells with intact p53 allele than in those...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02382-3

    authors： Zhang C,Gao C,Kawauchi J,Hashimoto Y,Tsuchida N,Kitajima S

    更新日期：2002-10-11 00:00:00

  • Cell cycle regulation of the BRCA1/acetyl-CoA-carboxylase complex.

    abstract：:Germ-line alterations in BRCA1 are associated with an increased susceptibility to breast and ovarian cancer. The BRCA1 protein has been implicated in multiple cellular functions. We have recently demonstrated that BRCA1 reduces acetyl-CoA-carboxylase alpha (ACCA) activity through its phospho-dependent binding to ACCA,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.11.090

    authors： Ray H,Suau F,Vincent A,Dalla Venezia N

    更新日期：2009-01-16 00:00:00

  • CircRNA circ-ITCH suppresses papillary thyroid cancer progression through miR-22-3p/CBL/β-catenin pathway.

    abstract：:While recent evidence has uncovered that circular RNAs (circRNAs) are vital regulators of carcinogenesis, their role in papillary thyroid cancer (PTC) is not clearly understood. In this study, we reveal that lower levels of circRNA circ-ITCH are expressed in PTC tissues than in normal adjacent tissues. Gain-of-functio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.08.175

    authors： Wang M,Chen B,Ru Z,Cong L

    更新日期：2018-09-26 00:00:00

  • Detection of phosphatidylserine surface exposure on human erythrocytes using annexin V-ferrofluid.

    abstract：:The asymmetric transbilayer distribution of phospholipids in the plasma membrane and the regulation of phosphatidylserine (PS) exposure at the cell surface of animal cells are of high physiological significance. It has been shown previously that annexin V is one of the most sensitive tools with which the presence of s...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0620

    authors： Geldwerth D,Helley D,de Jong K,Sabolovic' D,Sestier C,Roger J,Pons JN,Freyssinet JM,Devaux PF,Kuypers FA

    更新日期：1999-04-29 00:00:00

  • TNP-470 blockage of VEGF synthesis is dependent on MAPK/COX-2 signaling pathway in PDGF-BB-activated hepatic stellate cells.

    abstract：:Angiogenesis is a key pathogenic event in hepatic fibrogenesis, which is mediated by activated hepatic stellate cells (HSCs). TNP-470 is a known anti-angiogenic agent in cancer, and in this study, we investigated the regulatory mechanisms of TNP-470 blockage of vascular endothelial growth factor (VEGF) synthesis in ac...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.175

    authors： Wang YQ,Luk JM,Chu AC,Ikeda K,Man K,Kaneda K,Fan ST

    更新日期：2006-03-03 00:00:00

  • The antimicrobial action of histones in the reproductive tract of cow.

    abstract：:An infection of any part of female reproductive tract can severely interfere with fertility and reproduction. The fluids and epithelium from the lumen of the female reproductive tract (uterus, oviduct and ovarian follicle) are a known source of antimicrobial action in several species. In this study, we compared the an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.12.077

    authors： Dráb T,Kračmerová J,Hanzlíková E,Černá T,Litváková R,Pohlová A,Tichá M,Přikryl P,Liberda J

    更新日期：2014-01-17 00:00:00

  • Protein synthesis inhibition induces proteasome assembly and function.

    abstract：:Protein synthesis and degradation balance have a crucial role in maintenance of cellular homeostasis and function. The ubiquitin-proteasome system is one of the major cellular proteolytic machineries responsible for the removal of normal, abnormal, denatured or in general damaged proteins. Proteasome is a multisubunit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.114

    authors： Sakellari M,Chondrogianni N,Gonos ES

    更新日期：2019-06-18 00:00:00

  • Human autoantibodies against a nucleolar protein.

    abstract：:Autoimmune sera showing prominent immunofluorescence in nucleolus were selected and analysed by immunoblotting techniques. Immunoblots using a nucleolar extract as antigen source revealed sera recognizing a 38 kDa nucleolar protein. Low concentration of Actinomycin D, which inhibits the ribosomal RNA synthesis, caused...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92337-1

    authors： Kindås-Mügge I

    更新日期：1989-09-15 00:00:00

  • Structural and biochemical insights into inhibition of human primase by citrate.

    abstract：:The eukaryotic primase/polymerase complex synthesizes approximately 107 primers, one per Okazaki fragment, during the replication of mammalian chromosomes, which contain 109 base pairs. Primase catalyzes the synthesis of a short RNA segment to a single-stranded DNA template. Primase is important in DNA replication bec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.047

    authors： Lee JG,Park KR,An JY,Kang JY,Shen H,Wang J,Eom SH

    更新日期：2018-12-09 00:00:00

  • Activation of Nrf2 signaling by oltipraz inhibits death of human macrophages with mycobacterium tuberculosis infection.

    abstract：:Mycobacterium tuberculosis (MTB) infection can induce cytotoxicity to the host macrophages, promoting bacterial spread. We here tested the potential effect of oltipraz, a synthetic dithiolethione, in MTB-infected human macrophages. We show that oltipraz significantly inhibited MTB-induced death and apoptosis in human ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.07.026

    authors： Sun Q,Shen X,Ma J,Lou H,Zhang Q

    更新日期：2020-10-20 00:00:00

  • Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression.

    abstract：:The stability of mRNA influences the dynamics of gene expression. The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell proliferation, ap...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.02.007

    authors： Takahashi A,Kikuguchi C,Morita M,Shimodaira T,Tokai-Nishizumi N,Yokoyama K,Ohsugi M,Suzuki T,Yamamoto T

    更新日期：2012-03-09 00:00:00

  • Potentiation of monovalent cation effects on ligand binding to cardiac muscarinic receptors in N-ethylmaleimide treated membranes.

    abstract：:Guanine nucleotides and monovalent cations decrease the affinity of cardiac muscarinic receptors for agonists and are required for muscarinic receptor mediated inhibition of adenylate cyclase. N-ethylmaleimide abolished the effects of Gpp(NH)p on the ability of the agonist oxotremorine to inhibit the binding of the an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80114-4

    authors： McMahon KK,Hosey MM

    更新日期：1983-02-28 00:00:00

  • Promoter analysis and chromosomal mapping of human EBAG9 gene.

    abstract：:The human EBAG9 was previously identified as an estrogen responsive gene using CpG-genomic binding site cloning (Watanate et al., (1998) Mol. Cell. Biol. 18: 442-449). Recently it was revealed that the EBAG9 is identical with RCAS1 which is a cancer cell surface antigen implicated in immune escape. Here, we isolated a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2920

    authors： Ikeda K,Sato M,Tsutsumi O,Tsuchiya F,Tsuneizumi M,Emi M,Imoto I,Inazawa J,Muramatsu M,Inoue S

    更新日期：2000-07-05 00:00:00

  • Sulfated oxysterol, 25HC3S, is a potent regulator of lipid metabolism in human hepatocytes.

    abstract：:Recently, a novel oxysterol, 5-cholesten-3beta, 25-diol 3-sulfate (25HC3S) was identified in primary rat hepatocytes following overexpression of the cholesterol transport protein, StarD1. This oxysterol was also detected in human liver nuclei. In the present study, 25HC3S was chemically synthesized. Addition of 25HC3S...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.143

    authors： Ren S,Li X,Rodriguez-Agudo D,Gil G,Hylemon P,Pandak WM

    更新日期：2007-09-07 00:00:00

  • Oxidation-induced persistent activation of protein kinase C in hippocampal homogenates.

    abstract：:Incubation of purified protein kinase C with H2O2 results in the generation of a persistently activated form of the enzyme which is no longer dependent on Ca2+ or lipid cofactors. This oxidative activation of purified protein kinase C requires added Fe2+ in the incubation medium. Treatment of the soluble fraction of h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)90463-u

    authors： Palumbo EJ,Sweatt JD,Chen SJ,Klann E

    更新日期：1992-09-30 00:00:00

  • Cyclosporine downregulates Fas ligand expression on vascular endothelial cells: implication for accelerated vasculopathy by immunosuppressive therapy.

    abstract：:Although the introduction of cyclosporine A (CyA) and FK506 for immunosuppressive therapy has dramatically enhanced the early survival of organ transplant recipients, administration of these immunosuppresants is correlated with high incidence of transplant arteriosclerosis. Transplant-associated arteriosclerosis is be...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1392

    authors： Sata M,Walsh K

    更新日期：1999-09-24 00:00:00

  • Parathyroid hormone induces the nuclear orphan receptor NOR-1 in osteoblasts.

    abstract：:Parathyroid hormone (PTH) significantly affects osteoblast function by altering gene expression. We have identified neuron-derived orphan receptor-1 (NOR-1) as a PTH-induced primary gene in osteoblastic cells. NOR-1, Nurr1, and Nur77 comprise the NGFI-B nuclear orphan receptor family and Nurr1 and Nur77 are PTH-induce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00931-8

    authors： Pirih FQ,Nervina JM,Pham L,Aghaloo T,Tetradis S

    更新日期：2003-06-20 00:00:00

  • Identification of the domains required for the localization of Prp19p to lipid droplets or the nucleus.

    abstract：:Prp19p is a protein found in the nucleus, cytosol or lipid droplets depending on the cell type. Prp19p participates in pre-mRNA splicing, in neuronal/astroglial cell fate decisions or in adipocyte lipid droplet biogenesis. In this study, the motifs of Prp19p that are necessary for its localization to lipid droplets or...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.091

    authors： Cho SY,Park PJ,Lee JH,Kim JJ,Lee TR

    更新日期：2007-12-28 00:00:00

  • Reduction of PTP1B by RNAi upregulates the activity of insulin controlled fatty acid synthase promoter.

    abstract：:Metabolic deregulation accompanying type II diabetes is characterized by insulin resistance in peripheral tissues (liver, muscle, and adipose), mediated by impairments in insulin receptor (IR) signaling. Protein tyrosine phosphatase 1B (PTP1B) has been shown to be a negative regulator of IR autophosphorylation and thu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.02.016

    authors： Xu J,Li L,Qian Z,Hong J,Shen S,Huang W

    更新日期：2005-04-08 00:00:00

  • Nucleotide sequence of a bovine lens alpha A-crystallin cDNA.

    abstract：:We have determined the nucleotide sequence of a bovine lens alpha A2-crystallin cDNA clone, designated pBL alpha A2-1. The 793 bp cDNA insert contains coding information for the entire 173 amino acid alpha A2-crystallin polypeptide, as well as non-translated sequences located both upstream and downstream from the codi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91072-2

    authors： Hay RE,Petrash JM

    更新日期：1987-10-14 00:00:00

  • Identification of renin and renin messenger RNA sequence in rat ovary and uterus.

    abstract：:An increase in plasma prorenin during pregnancy suggests that prorenin might be synthesized in the ovary and the secretion of renin or prorenin may be stimulated by an ovarian steroid-mediated process. Recently, renin and angiotensinogen have been identified in human ovarian follicular fluid. However, there is conside...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90466-9

    authors： Kim S,Shinjo M,Fukamizu A,Miyazaki H,Usuki S,Murakami K

    更新日期：1987-01-15 00:00:00

  • Molecular cloning and characterization of rat lin-10.

    abstract：:In Caenorhabditis elegans, the vulval induction is mediated by tyrosine kinase receptor/Ras signal transduction pathway composed of the lin-3, let-23, and let-60 products. In addition to these gene products, the lin-2, lin-7, and lin-10 products are also implicated in this pathway. Lin-2 encodes a MAGUK and lin-7 enco...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8142

    authors： Ide N,Hirao K,Hata Y,Takeuchi M,Irie M,Yao I,Deguchi M,Toyoda A,Nishioka H,Mizoguchi A,Takai Y

    更新日期：1998-02-13 00:00:00

  • Induction of plasminogen activator activity by phorbol ester in transformed fetal bovine aortic endothelial cells. Possible independence from protein kinase C.

    abstract：:12-O-tetradecanoyl phorbol 13-acetate (TPA) and 1,2-dioctanoyl-sn-glycerol (diC8) activate protein kinase C (PKC) in transformed fetal bovine aortic endothelial GM 7373 cells. Both molecules cause a similar increase in membrane-associated PKC activity and in the phosphorylation of a PKC-specific endogenous 87-kDa subs...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92487-x

    authors： Maier JA,Presta M,Ragnotti G

    更新日期：1989-04-28 00:00:00

  • Cell surface trafficking of Fas in NIT-1 cells and dissection of surface and total Fas expression.

    abstract：:The appearance of Fas receptor at the surface of pancreatic beta-cells affected by progressive insulitis strongly suggests that Fas-mediated beta-cell apoptosis plays an important role in the pathogenesis of type 1 diabetes. In support of this concept, the present study has shown that islet cells from NOD mice and the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6215

    authors： Augstein P,Dunger A,Salzsieder C,Heinke P,Kubernath R,Bahr J,Fischer U,Rettig R,Salzsieder E

    更新日期：2002-01-11 00:00:00

  • ET-1 deletion from endothelial cells protects the kidney during the extension phase of ischemia/reperfusion injury.

    abstract：BACKGROUND:The prognosis of patients after acute kidney injury (AKI) is poor and treatment is limited. AKI is mainly caused by renal ischemia/reperfusion injury (IRI). During the extension phase of IRI, endothelial damage may participate in ischemia and inflammation. Endothelin-1 (ET-1) which is mostly secreted by endo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.07.121

    authors： Arfian N,Emoto N,Vignon-Zellweger N,Nakayama K,Yagi K,Hirata K

    更新日期：2012-08-24 00:00:00

  • The ras-binding domain of ral GDS-like protein-2 as a ras inhibitor in smooth muscle cells.

    abstract：:This study was undertaken to determine whether the response of smooth muscle cells to mitogens can be inhibited by inactivating ras with the ral GDS like protein-2 ras-binding domain (RGL2-RBD). RGL2 is a member of the ral GDS family of proteins that contains a carboxy terminal ras-binding domain which binds the GTP l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00878-7

    authors： Fischer TH,Brittain J,Trabalzini L,Banes AJ,White GC,Smith CJ,Nichols TC

    更新日期：2003-06-13 00:00:00

  • Cloning and characterization of the 5'-flanking region for the mouse phospholipase C-delta1 gene.

    abstract：:To date, little is known about the molecular mechanisms controlling the regulation of phospholipase C-delta1 (PLC-delta1) gene expression. To understand the mechanisms responsible for the regulation of PLC-delta1 gene expression, the 5'-flanking region of the mouse PLC-delta1 gene was isolated from a mouse genomic DNA...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2930

    authors： Kim JK,Lee WK,Nam HW,Lee KH,Han H,Rha HK,Jun TY,Kim KS,Choi CR

    更新日期：2000-06-24 00:00:00

  • Involvement of endogenous neuromedin U and neuromedin S in thermoregulation.

    abstract：:We investigated the possible involvement of neuromedin U (NMU) and neuromedin S (NMS) in thermoregulation in rats. Intracerebroventricular (icv) injection of NMU or NMS increased the back surface temperature (BS-T) in a dose-dependent manner during both the light and dark periods. Pre-treatment with the β3 blocker SR5...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.01.155

    authors： Nakahara K,Akagi A,Shimizu S,Tateno S,Qattali AW,Mori K,Miyazato M,Kangawa K,Murakami N

    更新日期：2016-02-19 00:00:00

  • Hypertonicity increases sodium transporters in cortical collecting duct cells independently of PGE2.

    abstract：:Cyclooxygenase-2 (COX-2) expression is increased by hypertonicity. Therefore we hypothesized that hypertonicity increased PGE(2) can modulate the sodium transporters (Na(+)/K(+)-ATPase: NKA, epithelial sodium channel: ENaC, and sodium hydrogen exchanger: NHE) in M1 cortical collecting duct (CCD) cells. We demonstrated...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.028

    authors： Nasrallah R,Paris G,Hébert RL

    更新日期：2012-02-10 00:00:00

  • Interaction between histone H1 and non-histone HMG14 detected by chemical cross-linking.

    abstract：:The interaction between histone H1 and non-histones HMG14 and HMG17 has been studied by chemical cross-linking. Cross-linking kinetics show the appearance of discrete bands which correspond to the interaction between H1 and HMG14. Interaction between H1 and HMG17 has not been detected. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91670-4

    authors： Espel E,Bernués J,Querol E,Martínez P,Barris A,Lloberas J

    更新日期：1983-12-28 00:00:00