当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Cloning and sequence of cDNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis.

Cloning and sequence of cDNA encoding a peptide C-terminal alpha-amidating enzyme from Xenopus laevis.

Abstract：

:The C-terminal alpha-amide formation of the peptides is one of the most important events of prohormone processing. We have recently isolated an alpha-amidating enzyme, AE-I, from Xenopus laevis skin, which is the only enzyme ever purified to homogeneity. In this study, we report cloning and sequence of cDNA encoding AE-I. Our results indicate that enzyme AE-I is initially synthesized as a precursor with 400 amino acid residues, which is further processed to the mature enzyme consisting of 344 residues. Preliminary expression in E. coli of the cDNA corresponding to AE-I was found to produce an enzyme with appreciable alpha-amidating activity.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Mizuno K,Ohsuye K,Wada Y,Fuchimura K,Tanaka S,Matsuo H

doi

10.1016/0006-291x(87)90911-9

subject

Has Abstract

pub_date

1987-10-29 00:00:00

pages

546-52

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)90911-9

journal_volume

148

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Effects of a protein kinase C inhibitor, K-252a, on human polymorphonuclear neutrophil responsiveness.

    abstract：:We investigated the capacity of K-252a, an inhibitor of rat brain protein kinase C (PKC), to influence polymorphonuclear neutrophil (PMN) PKC and PMN activation with chemically and structurally dissimilar agonists. K-252a inhibited PMN PKC (IC50 = 0.58 microM), and caused a concentration-dependent (0.1-10 microM) inhi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80455-8

    authors： Smith RJ,Justen JM,Sam LM

    更新日期：1988-05-16 00:00:00

  • A new and rapid method for visualising DNA replication in spread DNA by immunofluorescence detection of incorporated 5-iododeoxyuridine.

    abstract：:We have developed a new and rapid immunofluorescent method for visualisation of replicated regions on fixed DNA fibers. Using this method we have found in 5-fluorodeoxyuridine (FrdU)-blocked human cells distinct replication units covering about 60 kb of DNA and corresponding in size to single replicons or chromatin lo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1039

    authors： Tomilin N,Rosanov Yu,Zenin V,Bozhkov V,Vig B

    更新日期：1993-01-15 00:00:00

  • Expression of syntaxin 4 in rat skeletal muscle and rat skeletal muscle cells in culture.

    abstract：:Syntaxins are a family of membrane proteins believed to participate in docking/fusing of arriving vesicles during membrane sorting and secretion. Of the six mammalian syntaxins known, only brain syntaxin 1A/1B has been biochemically characterized in its endogenous form. Syntaxin 4 mRNA is expressed in selective tissue...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2154

    authors： Sumitani S,Ramlal T,Liu Z,Klip A

    更新日期：1995-08-15 00:00:00

  • Cysteine-106 of DJ-1 is the most sensitive cysteine residue to hydrogen peroxide-mediated oxidation in vivo in human umbilical vein endothelial cells.

    abstract：:Mutation in DJ-1 gene is the cause of autosomal recessive Parkinson's disease, however, its physiological function remains unclear. The isoelectric point of DJ-1 shows an acidic shift after cells are treated with hydrogen peroxide. This suggests that DJ-1 is modified in response to oxidative stress. Here we report the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.110

    authors： Kinumi T,Kimata J,Taira T,Ariga H,Niki E

    更新日期：2004-05-07 00:00:00

  • Differential modulation by N4, 2'-O-dibutyryl cytidine 3':5'-cyclic monophosphate of neutrophil activation.

    abstract：:The cyclic pyrimidine nucleotide, cCMP, is an endogenous substance in mammalian cells but little is known on its functional role. We studied the effects of cCMP, its cell-permeant analogue, N4,2'-O-dibutyryl cytidine 3':5'-cyclic monophosphate (Bt2cCMP), and of butyrate on superoxide (O2-) formation and cytosolic Ca2+...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90514-8

    authors： Ervens J,Seifert R

    更新日期：1991-01-15 00:00:00

  • Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA.

    abstract：:HMGB1 (high-mobility group B1) is a ubiquitously expressed bifunctional protein that acts as a nuclear protein in cells and also as an inflammatory mediator in the extracellular space. HMGB1 changes its functions according to the redox states in both intra- and extra-cellular environments. Two cysteines, Cys23 and Cys...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：

    authors： Wang J,Tochio N,Takeuchi A,Uewaki J,Kobayashi N,Tate S

    更新日期：2013-11-29 00:00:00

  • Purification of the 120 kDa component of the human nuclear factor of activated T cells (NF-AT): reconstitution of binding activity to the cis-acting element of the GM-CSF and IL-2 promoter with AP-1.

    abstract：:The cis-acting element of the granulocyte-macrophage colony-stimulating factor (GM-CSF) promoter, CLE0, is required for stimulation dependent expression of the GM-CSF gene by phorbol ester (PMA) and calcium ionophore (A23187) in T cells. We recently obtained evidence that NF-CLE0 gamma, one of the CLE0-binding factors...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2311

    authors： Tokumitsu H,Masuda ES,Tsuboi A,Arai K,Arai N

    更新日期：1993-10-29 00:00:00

  • Remodeling of ion channel expression may contribute to electrophysiological consequences caused by methamphetamine in vitro and in vivo.

    abstract：:Methamphetamine (MA) is a psychostimulant. MA may induce numerous cardiotoxic effects, leading to cardiac arrhythmias, heart failure, eventually leading to sudden cardiac death. The deleterious effects of methamphetamine work in tandem to disrupt the coordinated electrical activity of the heart and have been associate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.114

    authors： Qu YH,Leung KP,Qiao DF,Li DR,Liu C,Yue X,Wang HJ

    更新日期：2014-01-10 00:00:00

  • Interleukin-1: a cytokine that has potent antisecretory and anti-ulcer actions via the central nervous system.

    abstract：:The present study was performed to determine if interleukin-1 (IL-1) acts as a chemical messenger in the central nervous system (CNS) regulation of gastric secretion and ulcer formation, using male Wistar rats. The central injection of IL-1 dose-dependently inhibited gastric acid secretion in pylorus-ligated rats at 1...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80075-0

    authors： Uehara A,Okumura T,Kitamori S,Takasugi Y,Namiki M

    更新日期：1990-12-14 00:00:00

  • The stress-activated c-Jun protein kinase (JNK) is stimulated by lipoxygenase pathway product 12-HETE in RIN m5F cells.

    abstract：:Cytokine induced pancreatic beta-cell destruction seen in Type 1 diabetes and islet graft rejection involves multiple intracellular signaling pathways that directly or indirectly lead to inflammatory damage or programmed cell death. IL-1beta has been shown to stimulate the 12-lipoxygenase pathway product 12-HETE, in R...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.5981

    authors： Bleich D,Chen S,Wen Y,Nadler JL

    更新日期：1997-01-13 00:00:00

  • Prostaglandin E1 reduces the keratinocyte toxicity of sorafenib by maintaining signal transducer and activator of transcription 3 (STAT3) activity and enhancing the cAMP response element binding protein (CREB) activity.

    abstract：:Hand-foot skin reaction (HFSR) is a common side effect of multiple tyrosine kinase inhibitors (mTKIs). HFSR can necessitate dose reductions or interruption of therapy owing to its negative effect on the quality of life. Therefore, effective use of mTKIs requires measures to prevent HFSR. We evaluated the effect of pro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.02.107

    authors： Shichiri H,Yamamoto K,Tokura M,Ishida T,Uda A,Bito T,Nishigori C,Nakagawa T,Hirano T,Yano I,Hirai M

    更新日期：2017-04-01 00:00:00

  • Calcitonin gene-related peptide: an autocrine growth factor with regulatory activity in vitro.

    abstract：:We show that an autocrine system for calcitonin gene-related peptide (CGRP) exists in F9 teratocarcinoma cells. Synthesis of CGRP by F9 cells was demonstrated by measuring the peptide concentration in cells and medium and by determining specific mRNA in cells. During six days of culture, CGRP secretion did not vary si...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81504-9

    authors： Segond N,Gerbaud P,Cressent M,Lasmoles F,Taboulet J,Jullienne A,Raynaud F,Moukhtar MS,Evain-Brion D

    更新日期：1992-08-31 00:00:00

  • Photochemical intermediate of third rhodopsin-like pigment in Halobacterium halobium by simultaneous illumination with red and blue light.

    abstract：:We found new photochemical intermediate of third rhodopsin-like pigment (tR) or slow cycling rhodopsin-like pigment (sR) in Halobacterium halobium, which was produced by simultaneous illumination with red and blue light. This illumination is employed for measurements of negative phototaxis. The formation of this inter...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80231-4

    authors： Tomioka H,Kamo N,Takahashi T,Kobatake Y

    更新日期：1984-09-28 00:00:00

  • Separation of dolichol and dolichyl-P in microsomal and lysosomal compartments of hepatocytes.

    abstract：:The distribution, labeling and interrelationship of microsomal and lysosomal dolichol and dolichyl-P in rat liver was investigated. After membrane induction with phenobarbital, N-nitrosodiethylamine and diethylhexylphthalate, the amount of microsomal and lysosomal dolichols are modulated independently. Liposomal label...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80022-9

    authors： Edlund C,Chojnacki T,Dallner G

    更新日期：1983-09-30 00:00:00

  • Phosphorylation of calcineurin: effect on calmodulin binding.

    abstract：:The effect of phosphorylation of calcineurin on calmodulin (CaM) binding was examined using a synthetic peptide which contains the CaM-binding domain and the serine phosphorylation site. The peptide, corresponding to residues 391-414 of brain calcineurin A subunit, was rapidly phosphorylated by protein kinase C and Ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90708-u

    authors： Calalb MB,Kincaid RL,Soderling TR

    更新日期：1990-10-30 00:00:00

  • Nucleophile specificity in chymotrypsin peptide synthesis.

    abstract：:The partitioning of the acylenzyme acetyl-(Gly)n-Phe(NO2)-chymotrypsin (n = 0,1,2) to peptide and peptide acid is observed spectrophotometrically. Values of partitioning ratios for various nucleophiles are calculated from the spectral data. They are a measure for the "true" nucleophile reactivity and are useful in the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91103-3

    authors： Petkov DD,Stoineva I

    更新日期：1984-01-13 00:00:00

  • Reciprocal gene expression of osteoclastogenesis inhibitory factor and osteoclast differentiation factor regulates osteoclast formation.

    abstract：:Osteoblasts/stromal cells support the formation of osteoclast-like cells (OCL) from osteoclast progenitor cells via expressing a membrane-associated protein, osteoclast differentiation factor (ODF), in the presence of osteotropic factors, whereas the cells secrete a substantial amount of osteoclastogenesis inhibitory ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0524

    authors： Nagai M,Sato N

    更新日期：1999-04-21 00:00:00

  • A novel method to visually determine the intracellular pH of xenografted tumor in vivo by utilizing fluorescent protein as an indicator.

    abstract：:The alkalization of intracellular pH (pHin) advances together with enhancement of aerobic glycolysis within tumor cells (the Warburg effect), and that is responsible for the progression of tumor malignancy together with hypoxia and angiogenesis. But how they correlate each other during tumor growth is poorly understoo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.095

    authors： Tanaka S,Harada H,Hiraoka M

    更新日期：2015-09-04 00:00:00

  • Lupus autoantigen Ku protein binds HIV-1 TAR RNA in vitro.

    abstract：:The control of HIV-1 gene expression depends upon interaction of Tat with the trans-activation responsive (TAR) element present at the 5' end of all HIV-1 transcripts. The TAR sequence forms a stable hair-pin structure that binds Tat and several cellular factors in vitro. In the absence of Tat, TAR acts as a transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2339

    authors： Kaczmarski W,Khan SA

    更新日期：1993-10-29 00:00:00

  • Critical role of nucleotide-binding oligomerization domain-like receptor 3 in vascular repair.

    abstract：:Vascular remodeling characterized by hyperproliferative neointima formation is an unfavorable repair process that is triggered by vascular damage. This process is characterized by an increased local inflammatory and proliferative response that critically involves the pro-inflammatory cytokine interleukin-1β (IL-1β). I...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.07.006

    authors： Schlaweck S,Zimmer S,Struck R,Bartok E,Werner N,Bauernfeind F,Latz E,Nickenig G,Hornung V,Ghanem A

    更新日期：2011-08-05 00:00:00

  • HIV-1 gp120-induced apoptosis in the rat neocortex involves enhanced expression of cyclo-oxygenase type 2 (COX-2).

    abstract：:The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclooxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8321

    authors： Bagetta G,Corasaniti MT,Paoletti AM,Berliocchi L,Nisticò R,Giammarioli AM,Malorni W,Finazzi-Agrò A

    更新日期：1998-03-27 00:00:00

  • Expression and characterization of a fusion protein between the catalytic domain of poly(ADP-ribose) polymerase and the DNA binding domain of the glucocorticoid receptor.

    abstract：:A fusion protein comprising the DNA-binding region of the glucocorticoid receptor and the catalytic domain of poly(ADP-ribose) polymerase was constructed. This chimeric protein was expressed both in E. coli and in eukaryotic cells and was recognized by antibodies to both polymerase and the glucocorticoid receptor. Sim...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2012

    authors： Rosenthal D,Hong T,Cherney B,Zhang S,Shima T,Danielsen M,Smulson M

    更新日期：1994-07-29 00:00:00

  • Light-dependent regulation of chlorophyll b biosynthesis in chlorophyllide a oxygenase overexpressing tobacco plants.

    abstract：:Chlorophyllide a oxygenase (CAO) that converts chlorophyllide a to chlorophyllide b was overexpressed in tobacco to increase chlorophyll (Chl) b biosynthesis and alter the Chl a/b ratio. Transgenic plants along with their wild-type cultivars were grown in low and high light intensities. In low light there was 20% incr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.11.049

    authors： Pattanayak GK,Biswal AK,Reddy VS,Tripathy BC

    更新日期：2005-01-14 00:00:00

  • Effect of cysteamine on redox-sensitive thiol-containing proteins in the duodenal mucosa.

    abstract：:Recent studies from our laboratory demonstrated that Egr-1 is upregulated in the rat duodenal mucosa during cysteamine-induced duodenal ulceration and that antisense egr-1 oligonucleotide aggravates the duodenal ulcers. This study was aimed to determine the effects of cysteamine on redox-sensitive Egr-1 transcriptiona...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.08.092

    authors： Khomenko T,Deng X,Jadus MR,Szabo S

    更新日期：2003-10-03 00:00:00

  • Compensatory expression of p73 in PARP-deficient mouse fibroblasts as response to a reduced level of regularly spliced wild-type p53 protein.

    abstract：:We have investigated the effect of PARP gene inactivation on the expression of wild-type (wt) p53 protein. Using immortalized fibroblasts from control and PARP knock-out mice we have found by immunoblotting with the PAb421 antibody a profound decrease of the p53 expression to a barely detectable level in PARP knock-ou...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0191

    authors： Schmid G,Wang ZQ,Wesierska-Gadek J

    更新日期：1999-02-16 00:00:00

  • Recovery of function and mass of endogenous beta-cells in streptozotocin-induced diabetic rats treated with islet transplantation.

    abstract：:Islet transplantation corrects chronic hyperglycemia by augmentation of insulin supply from the graft tissue, but the role of endogenous beta-cells after transplantation is not clear. In the present study, we examined endogenous beta-cell function after glucose homeostasis had been reestablished by islet graft in stre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5563

    authors： Hamamoto Y,Tsuura Y,Fujimoto S,Nagata M,Takeda T,Mukai E,Fujita J,Yamada Y,Seino Y

    更新日期：2001-09-14 00:00:00

  • Effect of alpha 1-proteinase inhibitor and sulphated polysaccharides on the activity of m beta-acrosin.

    abstract：:Purified m beta-acrosin catalysed amidolysis in vitro of several p-nitroanilides with C-terminal arginine residues. alpha 1-proteinase inhibitor inhibited amidolysis catalysed by the enzyme. This effect of alpha 1-Proteinase inhibitor was not prevented by pre-incubation of the enzyme with heparin or any other glycosam...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91578-4

    authors： Long WF,Williamson FB

    更新日期：1983-11-30 00:00:00

  • Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides.

    abstract：:We have recently identified in porcine brain a series of new peptides, designated calcitonin receptor-stimulating peptide-1 (CRSP-1), CRSP-2, and CRSP-3, but failed to find their counterparts in humans and rodents by either database searching or experimental cross-hybridization. In this study, we isolated cDNAs encodi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.11.114

    authors： Katafuchi T,Hamano K,Minamino N

    更新日期：2004-01-02 00:00:00

  • Molecular force spectroscopy of homophilic nectin-1 interactions.

    abstract：:Nectins are Ca2+ independent cell adhesion molecules localizing at the cadherin based adherens junctions. In this study, we have used atomic force microscopy to study interaction of a chimera of extra cellular fragment of nectin-1 and Fc of human IgG (nef-1) with wild type L-fibroblasts that express endogenous nectin-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.08.096

    authors： Vedula SR,Lim TS,Hui S,Kausalya PJ,Lane EB,Rajagopal G,Hunziker W,Lim CT

    更新日期：2007-11-03 00:00:00

  • The eta isoform of protein kinase C inhibits UV-induced activation of caspase-3 in normal human keratinocytes.

    abstract：:Protein kinase C (PKC) fulfills a central role in the decision of cell fate in keratinocytes. Both PKC delta and PKC eta induce growth inhibition and differentiation of normal human keratinocytes (NHK). Here we show that PKC delta and PKC eta play opposite roles in UVB-induced apoptosis in NHK. PKC delta enhanced UVB-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)00345-0

    authors： Matsumura M,Tanaka N,Kuroki T,Ichihashi M,Ohba M

    更新日期：2003-03-28 00:00:00