当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > A new and rapid method for visualising DNA replication in spread DNA by immunofluorescence detection of incorporated 5-iododeoxyuridine.

A new and rapid method for visualising DNA replication in spread DNA by immunofluorescence detection of incorporated 5-iododeoxyuridine.

Abstract：

:We have developed a new and rapid immunofluorescent method for visualisation of replicated regions on fixed DNA fibers. Using this method we have found in 5-fluorodeoxyuridine (FrdU)-blocked human cells distinct replication units covering about 60 kb of DNA and corresponding in size to single replicons or chromatin loops. Our results also suggest that nonadjacent replicons within a replicon cluster may be activated after FrdU arrest and that the method may be adapted for localization on fibers of specific DNA sequences.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tomilin N,Rosanov Yu,Zenin V,Bozhkov V,Vig B

doi

10.1006/bbrc.1993.1039

subject

Has Abstract

pub_date

1993-01-15 00:00:00

pages

257-62

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006291X83710399

journal_volume

190

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Expression and possible functional roles of cytochromes P450 2J1 (zfCyp 2J1) in zebrafish.

    abstract：:Cytochrome P450 2J (Cyp2J) subfamilies are recognized as catalysts of arachidonic acid metabolism in extrahepatic tissues of many species. However, to date, no P450 2J have been identified in zebrafish. We describe here a zfCyp2J1 cDNA which encodes a putative protein of 496 amino acids and shares 51%, 51%, 50%, 51% a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.129

    authors： Wang L,Yao J,Chen L,Chen J,Xue J,Jia W

    更新日期：2007-01-26 00:00:00

  • Genomic organization, chromosomal localization, and expression of the murine RAB3D gene.

    abstract：:Rab proteins, members of the Ras superfamily of small GTPases, play regulatory roles in intercompartmental vesicular transport. Each step of traffic seems to require the participation of at least one distinct Rab, with the Rab3 subfamily involved in stimulated exocytosis. We report our studies on the murine rab3D gene...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3032

    authors： Adachi R,Nigam R,Tuvim MJ,DeMayo F,Dickey BF

    更新日期：2000-07-14 00:00:00

  • CAAT/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter.

    abstract：:The CCAAT/enhancer binding proteins (C/EBPs) and the peroxisome proliferator-activated receptors (PPARs) together regulate adipogenesis. The current work uses co-transfection studies to examine the C/ EBP dependence of PPAR gamma 2 transcription. Both C/ EBP alpha and C/EBP delta expression vectors activated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7627

    authors： Clarke SL,Robinson CE,Gimble JM

    更新日期：1997-11-07 00:00:00

  • BAG3 affects the nucleocytoplasmic shuttling of HSF1 upon heat stress.

    abstract：:Bcl2-associated athoanogene (BAG) 3 is a member of the co-chaperone BAG family. It is induced by stressful stimuli such as heat shock and heavy metals, and it regulates cellular adaptive responses against stressful conditions. In this study, we identified a novel role for BAG3 in regulating the nuclear shuttling of HS...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.006

    authors： Jin YH,Ahn SG,Kim SA

    更新日期：2015-08-21 00:00:00

  • The ribonuclease inhibitors from porcine thyroid and liver are slow, tight-binding inhibitors of bovine pancreatic ribonuclease A.

    abstract：:Ribonuclease inhibitors were purified from the latent ribonuclease fractions of porcine thyroid and liver and used to test the hypothesis that their inhibition of bovine pancreatic ribonuclease A is correctly described by tight-binding rather than Michaelis-Menton kinetics. Both proteins were found to act as slow, tig...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90683-6

    authors： Turner PM,Lerea KM,Kull FJ

    更新日期：1983-08-12 00:00:00

  • Disulfiram suppresses cancer stem-like properties and STAT3 signaling in triple-negative breast cancer cells.

    abstract：:In the presence of copper (Cu), disulfiram (DSF) suppresses properties associated with cancer stem cells (CSCs) in breast cancer, but the mechanism of action is poorly understood. In the present study, we observed that DSF/Cu treatment induced apoptosis, mediated by caspase-3 activation in triple-negative breast cance...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.164

    authors： Kim YJ,Kim JY,Lee N,Oh E,Sung D,Cho TM,Seo JH

    更新日期：2017-05-13 00:00:00

  • Parathyroid hormone increases CFTR expression and function in Caco-2 intestinal epithelial cells.

    abstract：:Parathyroid hormone (PTH) enhances cystic fibrosis transmembrane conductance regulator (CFTR)-mediated anion secretion by the human intestinal epithelial cell line Caco-2. With the patch-clamp and Ussing chamber techniques, we investigated how PTH stimulates CFTR activity in Caco-2 cells. Cell-attached recordings reve...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.12.106

    authors： Jantarajit W,Wongdee K,Lertsuwan K,Teerapornpuntakit J,Aeimlapa R,Thongbunchoo J,Harvey BSJ,Sheppard DN,Charoenphandhu N

    更新日期：2020-03-12 00:00:00

  • Cholesterol attenuated the progression of DEN-induced hepatocellular carcinoma via inhibiting SCAP mediated fatty acid de novo synthesis.

    abstract：:Worldwide, hepatocellular carcinoma (HCC) remains a top instigator of cancer mortality. Previous clinical studies have revealed that low serum cholesterol predicts a poor outcome in HCC patients, but the potential role of cholesterol in the progression of HCC remains controversial. In the present study,we tested the i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.12.181

    authors： Zhao Z,Zhong L,He K,Qiu C,Li Z,Zhao L,Gong J

    更新日期：2019-02-19 00:00:00

  • Purification of the insulin-like growth factor II (IGF-II) receptor from an IGF-II-producing cell line, and generation of an antibody which both immunoprecipitates and blocks the type 2 IGF receptor.

    abstract：:18,54-SF cells, which secrete rat insulin-like growth factor II (rIGF-II), have abundant type 2 IGF receptors. We have purified the type 2 receptor from these cells by solubilization of crude membranes in Triton X-100, followed by chromatography on agarose-immobilized rIGF-II. A partially purified receptor preparation...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90280-9

    authors： Rosenfeld RG,Hodges D,Pham H,Lee PD,Powell DR

    更新日期：1986-07-16 00:00:00

  • Desensitization to norepinephrine includes refractoriness of calcium release in myocardial cells.

    abstract：:Localization of myoplasmic free calcium was measured in fura2-loaded single rat myocardial cells to determine whether the mechanism of norepinephrine desensitization includes redistribution of calcium. Fluorescence intensities at each pixel were quantitated by use of a photon-counting, microchannel plate camera. From ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90598-0

    authors： Erne P,Hermsmeyer K

    更新日期：1988-02-29 00:00:00

  • A severe de novo methylation of episomal vectors by human ES cells.

    abstract：:Episomal vectors can allow efficient genetic modification of cells and have the potential advantage of avoiding chromosomal position of integration effects. Here we explore the use of an Epstein-Barr virus-based episomal vector with human embryonic stem (ES) cells, and find high initial transfection rates, but a rapid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.08.175

    authors： Kameda T,Smuga-Otto K,Thomson JA

    更新日期：2006-11-03 00:00:00

  • Androgen receptor regulates cardiac fibrosis in mice with experimental autoimmune myocarditis by increasing microRNA-125b expression.

    abstract：:Cardiac fibrosis is an important cardiac remodeling event in the development of inflammation dilated cardiomyopathy （iDCM）. We have previously observed that degradation enhancer of androgen receptor (ASC-J9®) could improve cardiac inflammation and fibrosis. Using Primary CFs, we demonstrated that ASC-J9® attenuates th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.09.092

    authors： Wang Y,Ma W,Lu S,Yan L,Hu F,Wang Z,Cheng B

    更新日期：2018-11-17 00:00:00

  • Regulated secretory proteins in the exocrine pancreas aggregate under conditions that mimic the trans-Golgi network.

    abstract：:Fifteen pancreatic secretory proteins, including seven serine-endoproteinases (isoenzyme forms of trypsinogen, chymotrypsinogen and proelastase), four metallo-exoproteinases (isoenzymic forms of procarboxypeptidase A and procarboxypeptidase B), amylase, lipase, and two forms of carboxyl ester lipase were observed to a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2577

    authors： Freedman SD,Scheele GA

    更新日期：1993-12-15 00:00:00

  • Oxylipin formation in fungi: biotransformation of arachidonic acid to 3-hydroxy-5,8-tetradecadienoic acid by Mucor genevensis.

    abstract：:The soil fungus Mucor genevensis was shown to convert exogenous arachidonic acid to the oxylipin 3-hydroxy-5Z,8Z-tetradecadienoic acid (3-HTDE) as determined by gas chromatography/mass spectrometry. This metabolite was only found in the aqueous supernatant together with free linoleic acid, but not in the final fungal ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9853

    authors： Pohl CH,Botha A,Kock JL,Coetzee DJ,Botes PJ,Schewe T,Nigam S

    更新日期：1998-12-30 00:00:00

  • Binding specificity of lung surfactant protein SP-D for glucosylceramide.

    abstract：:The specificities of the binding of lung surfactant protein SP-D to glycolipids were examined using 125I-labeled SP-D as a probe. When the binding study was performed on TLC plates, SP-D bound exclusively to GlcCer, whereas it failed to bind to GalCer, GM1, GM2, asialo-GM1, asialo-GM2, sulfatide, Forssman antigen, cer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91291-w

    authors： Kuroki Y,Gasa S,Ogasawara Y,Shiratori M,Makita A,Akino T

    更新日期：1992-09-16 00:00:00

  • Icariin stimulates angiogenesis by activating the MEK/ERK- and PI3K/Akt/eNOS-dependent signal pathways in human endothelial cells.

    abstract：:We investigated the molecular effect and signal pathway of icariin, a major flavonoid of Epimedium koreanum Nakai, on angiogenesis. Icariin stimulated in vitro endothelial cell proliferation, migration, and tubulogenesis, which are typical phenomena of angiogenesis, as well as increased in vivo angiogenesis. Icariin a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.09.001

    authors： Chung BH,Kim JD,Kim CK,Kim JH,Won MH,Lee HS,Dong MS,Ha KS,Kwon YG,Kim YM

    更新日期：2008-11-14 00:00:00

  • A novel nucleic acid analogue shows strong angiogenic activity.

    abstract：:A novel nucleic acid analogue (2Cl-C.OXT-A) significantly stimulated tube formation of human umbilical endothelial cells (HUVEC). Its maximum potency at 100muM was stronger than that of vascular endothelial growth factor (VEGF), a positive control. At this concentration, 2Cl-C.OXT-A moderately stimulated proliferation...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.08.003

    authors： Tsukamoto I,Sakakibara N,Maruyama T,Igarashi J,Kosaka H,Kubota Y,Tokuda M,Ashino H,Hattori K,Tanaka S,Kawata M,Konishi R

    更新日期：2010-09-03 00:00:00

  • Three novel PHEX gene mutations in four Chinese families with X-linked dominant hypophosphatemic rickets.

    abstract：BACKGROUND:X-linked hypophosphatemia (XLH), the most common form of inherited rickets, is a dominant disorder that is characterized by renal phosphate wasting with hypophosphatemia, abnormal bone mineralization, short stature, and rachitic manifestations. The related gene with inactivating mutations associated with XLH...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.042

    authors： Kang QL,Xu J,Zhang Z,He JW,Lu LS,Fu WZ,Zhang ZL

    更新日期：2012-07-13 00:00:00

  • Tumor necrosis factor (TNF) interferes with insulin signaling through the p55 TNF receptor death domain.

    abstract：:Tumor necrosis factor (TNF) contributes to insulin resistance by binding to the 55kDa TNF receptor (TNF-R55), resulting in serine phosphorylation of proteins such as insulin receptor (IR) substrate (IRS)-1, followed by reduced tyrosine phosphorylation of IRS-1 through the IR and, thereby, diminished IR signal transduc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.140

    authors： Csehi SB,Mathieu S,Seifert U,Lange A,Zweyer M,Wernig A,Adam D

    更新日期：2005-04-01 00:00:00

  • Bortezomib prevents the expression of MMP-13 and the degradation of collagen type 2 in human chondrocytes.

    abstract：:The structural backbone of extracellular matrix in cartilage is the collagen fibril, which is mainly composed of type II collagen. A measurable increase in type II collagen denaturation and degradation has been found in early Osteoarthritis (OA). Pro-inflammatory cytokine such as TNF-α produced in OA cartilage induced...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.08.102

    authors： Hu W,Zhang W,Li F,Guo F,Chen A

    更新日期：2014-09-26 00:00:00

  • WITHDRAWN: Epigallocatechin-3-gallate up-regulates tumor suppressor gene expression via a reactive oxygen species-dependent down-regulation of UHRF1.

    abstract：:This article has been withdrawn at the request of the editors. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.130

    authors： Achour M,Mousli M,Alhosin M,Ibrahim A,Peluso J,Muller CD,Schini-Kerth VB,Hamiche A,Dhe-Paganon S,Christian Bronner

    更新日期：2012-07-04 00:00:00

  • The role of DNA hypomethylation in the control of stromelysin gene expression.

    abstract：:Genome-wide DNA hypomethylation is a critical mechanism underlying neoplastic transformation. Thus, genes that are suppressed in normal tissues or in specific cell types may become aberrantly expressed in neoplasia. To determine whether DNA methylation can modulate matrix metalloproteinase (mmp) gene expression, we ha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.068

    authors： Couillard J,Demers M,Lavoie G,St-Pierre Y

    更新日期：2006-04-21 00:00:00

  • Wnt activator CHIR99021-stimulated human dermal papilla spheroids contribute to hair follicle formation and production of reconstituted follicle-enriched human skin.

    abstract：:The aim of the present study was to accomplish de novo generation of reconstituted human skin with enriched hair follicles. Dermal papillae (DP) are known to play a crucial organizing role in hair follicle induction. However, generation of enriched human hair follicles using cultured DP cells has not been accomplished...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.038

    authors： Yoshida Y,Soma T,Matsuzaki T,Kishimoto J

    更新日期：2019-08-27 00:00:00

  • FGF-23 transgenic mice demonstrate hypophosphatemic rickets with reduced expression of sodium phosphate cotransporter type IIa.

    abstract：:Fibroblast growth factor (FGF)-23 was identified as a causative factor of tumor-induced osteomalacia and also as a responsible gene for autosomal dominant hypophosphatemic rickets. To clarify the pathophysiological roles of FGF-23 in these diseases, we generated its transgenic mice. The transgenic mice expressing huma...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.12.102

    authors： Shimada T,Urakawa I,Yamazaki Y,Hasegawa H,Hino R,Yoneya T,Takeuchi Y,Fujita T,Fukumoto S,Yamashita T

    更新日期：2004-02-06 00:00:00

  • Transient activation of c-myc protooncogene expression in Leydig cells by human chorionic gonadotropin.

    abstract：:This study evaluated the effects of in vivo administration of human chorionic gonadotropin (hCG) on c-myc oncogene expression in Leydig cells. Sprague-Dawley rats (46-50 days old) were treated with hCG (10 units, i.p.), and purified Leydig cells were isolated 1-24 h later. HCG caused a transient elevation of c-myc mRN...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80021-4

    authors： Lin T,Blaisdell J,Barbour KW,Thompson EA

    更新日期：1988-11-30 00:00:00

  • A selective c-Fos/AP-1 inhibitor prevents cartilage destruction and subsequent osteophyte formation.

    abstract：:The objective of the present study is to demonstrate that a newly developed selective c-Fos/activator protein (AP)-1 inhibitor, T-5224, inhibits the expression of matrix metalloproteinases (MMPs) in human articular chondrocytes, and prevents cartilage destruction in an osteoarthritis (OA)-induced mouse model. First, w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.147

    authors： Motomura H,Seki S,Shiozawa S,Aikawa Y,Nogami M,Kimura T

    更新日期：2018-03-04 00:00:00

  • A new approach to possible substrate binding mechanisms for nitrile hydratase.

    abstract：:We combined normal mode analysis (NMA) with cavity calculations as a method to get more insight into static crystal structures. We used nitrile hydratase (NHase) as a case study, and the crystal structure of a complex of Pseudonocardia thermophila NHase (1UGP) with n-butyric acid was chosen as a reference structure. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.02.150

    authors： Taştan Bishop AO,Sewell T

    更新日期：2006-04-28 00:00:00

  • Attenuation of experimental autoimmune uveoretinitis in mice by IKKβ inhibitor IMD-0354.

    abstract：:Uveitis is a sight-threatening intraocular inflammatory disease that accounts for almost 10% of blindness worldwide. NF-κB signaling plays pivotal roles in inflammatory diseases. We have reported that IMD-0354, which inhibits NF-κB signaling via selective blockade of IKK-β, suppresses inflammation in several ocular di...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.117

    authors： Liu Y,Kitaichi N,Wu D,Hase K,Satoh M,Iwata D,Namba K,Kanda A,Noda K,Itai A,Iwabuchi K,Ishida S

    更新日期：2020-05-07 00:00:00

  • microRNA-146a inhibits proliferation, migration and invasion of human cervical and colorectal cancer cells.

    abstract：:microRNAs (miRNAs) play significant roles in diverse biological processes and their deregulation is implicated in carcinogenesis. miR-146a executes tumour suppressive or oncogenic functions depending on the cancer type, but its effect on human cervical (CaCx) and colorectal (CRC) cancers have not been examined thus fa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.10.054

    authors： Sathyanarayanan A,Chandrasekaran KS,Karunagaran D

    更新日期：2016-11-25 00:00:00

  • Functional characterization of Brassica napus DNA topoisomerase Iα-1 and its effect on flowering time when expressed in Arabidopsis thaliana.

    abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.011

    authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

    更新日期：2017-04-22 00:00:00