Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides.


:We have recently identified in porcine brain a series of new peptides, designated calcitonin receptor-stimulating peptide-1 (CRSP-1), CRSP-2, and CRSP-3, but failed to find their counterparts in humans and rodents by either database searching or experimental cross-hybridization. In this study, we isolated cDNAs encoding precursors of bovine CRSP-1, canine CRSP-1, and canine CRSP-2 from their thyroid cDNA libraries. Although the deduced mature amino acid sequences of bovine and canine CRSP-1s and canine CRSP-2 showed identity with their respective porcine CRSP counterparts, none of them had a C-terminal amide structure. In LLC-PK(1) cells endogenously expressing the calcitonin (CT) receptor, bovine and canine CRSP-1s enhanced the cAMP production, while canine CRSP-2 did not stimulate it at all. Equine CGRP-I had a high identity in its amino acid sequence with porcine CRSP-1 and stimulated LLC-PK(1) cells at a potency comparable to that of porcine CT. None of these CRSPs or equine CGRP-I stimulated the CT-like receptor, even in the presence of receptor activity-modifying proteins. These results demonstrate that CRSP-1, a new class of biologically active peptide, is present in animals evolutionarily close to pigs and induces its activity through the calcitonin receptor, suggesting a wide existence and common properties of this peptide in mammals.


Katafuchi T,Hamano K,Minamino N




Has Abstract


2004-01-02 00:00:00














  • Ca2+-ATPase inhibitors and Ca2+-ionophore induce osteoclast-like cell formation in the cocultures of mouse bone marrow cells and calvarial cells.

    abstract::Osteoclasts which derive from hemopoietic cells are multinucleated cells responsible for bone resorption. We found that cyclopiazonic acid (CPA), thapsigargin (TG), and 2,5-di-(t-butyl)-1,4-hydroquinone (BHQ) induced osteoclast-like cell (OCL) formation in cocultures of mouse calvaria-derived stromal cells and hemopoi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takami M,Woo JT,Takahashi N,Suda T,Nagai K

    更新日期:1997-08-08 00:00:00

  • Hormonal effects on the development changes of mouse small intestinal glycolipids.

    abstract::The composition of intestinal glycosphingolipids during normal and hormone-perturbed development was investigated. The concentrations of glycosphingolipids of mouse small intestine were affected by the injection of thyroxine or cortisone during suckling and weaning periods. GDla was reduced by the hormonal treatment a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sato E,Fujie M,Uezato T,Fujita M,Nishimura K

    更新日期:1984-03-30 00:00:00

  • Calcitonin gene-related peptide(CGRP) stimulates the release of atrial natriuretic peptide(ANP) from isolated rat atria.

    abstract::The effect of calcitonin gene-related peptide(CGRP) on the release of atrial natriuretic peptide(ANP) was studied in spontaneously beating, isolated rat atria. CGRP stimulated the ANP release in a dose-dependent manner. When the atria were incubated with a combination of phentolamine, propranolol, and atropine, these ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yamamoto A,Kimura S,Hasui K,Fujisawa Y,Tamaki T,Fukui K,Iwao H,Abe Y

    更新日期:1988-09-30 00:00:00

  • IL-10 inhibits Porphyromonas gingivalis LPS-stimulated human gingival fibroblasts production of IL-6.

    abstract::Lipopolysaccharides (LPS) of Porphyromonas gingivalis have been implicated in the initiation and development of periodontal diseases. In a previous study, we investigated the signal transduction pathway of P. gingivalis and demonstrated that LPS stimulates the production of interleukin (IL)-6 in human gingival fibrobl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang PL,Shirasu S,Shinohar M,Azuma Y,Daito M,Yasuda H,Ohura K

    更新日期:1999-09-24 00:00:00

  • Stage-specific expression of testis-specific protein kinase 1 (TESK1) in rat spermatogenic cells.

    abstract::TESK1 (testis-specific protein kinase 1) is a serine/threonine kinase, with a unique structure composed of an N-terminal protein kinase domain and a C-terminal proline-rich domain. Northern blot analysis revealed that TESK1 mRNA is predominantly expressed in testicular germ cells. We present here evidence that express...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Toshima J,Koji T,Mizuno K

    更新日期:1998-08-10 00:00:00

  • C-type natriuretic peptide is a growth inhibitor of rat vascular smooth muscle cells.

    abstract::C-type natriuretic peptide (CNP) which potently stimulates particulate guanylate cyclase activity in cultured rat vascular smooth muscle cells (VSMC) inhibited serum-induced DNA synthesis of the cells 10-fold more effectively than alpha-human atrial natriuretic peptide (alpha-hANP). The inhibitory effect of CNP was mi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Furuya M,Yoshida M,Hayashi Y,Ohnuma N,Minamino N,Kangawa K,Matsuo H

    更新日期:1991-06-28 00:00:00

  • Effect of advanced glycation end products on lens epithelial cells in vitro.

    abstract::The extended exposure of proteins to reducing sugars leads to nonenzymatic glycation with the accumulation of advanced glycation end products (AGEs). Long-lived proteins, such as collagen and crystallins, are subjected to this modification, and are implicated as causal factors in several diseases including diabetic co...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hong SB,Lee KW,Handa JT,Joo CK

    更新日期:2000-08-18 00:00:00

  • Isolation of rat adrenocortical mitochondria.

    abstract::This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Solinas P,Fujioka H,Tandler B,Hoppel CL

    更新日期:2012-10-12 00:00:00

  • Sp1 mediates repression of the resistin gene by PPARgamma agonists in 3T3-L1 adipocytes.

    abstract::Resistin is an adipokine related to obesity and insulin resistance. Expression of the resistin gene is repressed by the treatment of peroxisome proliferator-activated receptor gamma (PPARgamma) agonists, thiazolidinediones (TZDs). In this study, we investigated the mechanism by which TZDs inhibit the resistin gene exp...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Chung SS,Choi HH,Cho YM,Lee HK,Park KS

    更新日期:2006-09-15 00:00:00

  • Cholecystokinin-induced redistribution of paxillin in rat pancreatic acinar cells.

    abstract::Cholecystokinin (CCK) dose-dependently stimulates enzyme secretion or loss of cell integrity in the exocrine pancreas. Signaling mechanims include tyrosine phosphorylation of p125(FAK) and paxillin. Here, we examine their potential function. Maximum phosphorylation of both proteins was observed after stimulation of fr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Leser J,Lührs H,Beil MF,Adler G,Lutz MP

    更新日期:1999-01-19 00:00:00

  • Antifungal activity of lariciresinol derived from Sambucus williamsii and their membrane-active mechanisms in Candida albicans.

    abstract::Lariciresinol is an enterolignan precursor isolated from the herb Sambucus williamsii, a folk medicinal plant used for its therapeutic properties. In this study, the antifungal properties and mode of action of lariciresinol were investigated. Lariciresinol displays potent antifungal properties against several human pa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hwang B,Cho J,Hwang IS,Jin HG,Woo ER,Lee DG

    更新日期:2011-07-08 00:00:00

  • COBRA1 inhibits AP-1 transcriptional activity in transfected cells.

    abstract::Mutations in the breast cancer susceptibility gene (BRCA1) account for a significant proportion of hereditary breast and ovarian cancers. Cofactor of BRCA1 (COBRA1) was isolated as a BRCA1-interacting protein and exhibited a similar chromatin reorganizing activity to that of BRCA1. However, the biological role of COBR...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhong H,Zhu J,Zhang H,Ding L,Sun Y,Huang C,Ye Q

    更新日期:2004-12-10 00:00:00

  • The effect of myotonic dystrophy transcript levels and location on muscle differentiation.

    abstract::In myotonic dystrophy type I (DM1), nuclear retention of mutant DMPK transcripts compromises muscle cell differentiation. Although several reports have identified molecular defects in myogenesis, it remains still unclear how exactly the retention of the mutant transcripts induces this defect. We have recently created ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mastroyiannopoulos NP,Chrysanthou E,Kyriakides TC,Uney JB,Mahadevan MS,Phylactou LA

    更新日期:2008-12-12 00:00:00

  • Determination of type I transglutaminase in differentiating normal and neoplastic human keratinocytes by an in situ radioimmunoassay.

    abstract::The presence of type I transglutaminase was determined in the neoplastic human keratinocyte line SqCC/Y1 and in normal human epidermal keratinocytes (NHEK) by an in situ radioimmunoassay which corresponded directly with the measurement of type I transglutaminase enzymatic activity. Dexamethasone induced differentiatio...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Moore KG,Donadio AC,Sartorelli AC

    更新日期:1993-04-30 00:00:00

  • Hyperbaric oxygen selectively induces angiopoietin-2 in human umbilical vein endothelial cells.

    abstract::One of the biological effects of hyperbaric oxygen (HBO) therapy in enhancing ischemia-related wound healing is the induction of angiogenesis. To elucidate the mechanism(s) underlying the HBO-induced angiogenesis, we studied the expression of several angiogenesis-related genes in human umbilical vein endothelial cells...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin S,Shyu KG,Lee CC,Wang BW,Chang CC,Liu YC,Huang FY,Chang H

    更新日期:2002-08-23 00:00:00

  • Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species.

    abstract::Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced prote...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pride H,Yu Z,Sunchu B,Mochnick J,Coles A,Zhang Y,Buffenstein R,Hornsby PJ,Austad SN,Pérez VI

    更新日期:2015-02-20 00:00:00

  • Diacylglycerol causes major structural transitions in phospholipid bilayer membranes.

    abstract::We demonstrate for the first time that major structural changes are imposed on various phospholipid bilayers by diacylglycerol, a product of phosphatidylinositol metabolism. By 5 mole percent in phosphatidylethanolamine a lamellar to hexagonal transition starts that is complete at 10 mole percent. At 30 mole percent i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Das S,Rand RP

    更新日期:1984-10-30 00:00:00

  • Hairpin antisense oligonucleotides containing 2'-methoxynucleosides with base-pairing in the stem region at the 3'-end: penetration, localization, and Anti-HIV activity.

    abstract::Hairpin antisense oligodeoxyribonucleotides containing 2'-methoxynucleosides were more active in the micromolar concentration range than linear and DNA hairpin phosphorothioate oligonucleotides with the same sequence. Furthermore, the abilities of hairpin antisense and random hairpin phosphorothioate oligonucleotides ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuwasaki T,Hosono K,Takai K,Ushijima K,Nakashima H,Saito T,Yamamoto N,Takaku H

    更新日期:1996-11-12 00:00:00

  • Variation in Chst8 gene expression level affects PrPC to PrPSc conversion efficiency in prion-infected Mov cells.

    abstract::The conversion of the endogenous cellular prion protein to an abnormally folded isoform is a hallmark of transmissible spongiform encephalopathies. It occurs when a misfolded prion protein contacts the cellular PrP. Among the molecular partners suggested to be involved in the misfolding process, the glycosaminoglycans...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Martin R,Chantepie S,Chapuis J,Le-Duc A,Maftah A,Papy-Garcia D,Laude H,Petit JM,Gallet PF

    更新日期:2011-10-28 00:00:00

  • Basal mTORC2 activity and expression of its components display diurnal variation in mouse perivascular adipose tissue.

    abstract::In adipose tissue mTOR complex 2 (mTORC2) contributes to the regulation of glucose/lipid metabolism and inflammatory molecule expression. Both processes display diurnal variations during the course of the day. RICTOR and mSIN1 are unique and essential components of mTORC2, which is activated by growth factors includin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Drägert K,Bhattacharya I,Hall MN,Humar R,Battegay E,Haas E

    更新日期:2016-04-22 00:00:00

  • Glutathione S-transferase in yeast: induction of mRNA, cDNA cloning and expression in Escherichia coli.

    abstract::Glutathione S-transferase Y-2 mRNA synthesis was induced in yeast Issatchenkia orientalis approximately 37-fold by cultivation with o-dinitrobenzene. A cDNA library complementary to poly (A)+RNA of I. orientalis grown with o-dinitrobenzene was screened by colony hybridization. Twenty positive clones were obtained from...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tamaki H,Kumagai H,Tochikura T

    更新日期:1990-10-30 00:00:00

  • The integrated stress response and proteotoxicity in cancer therapy.

    abstract::A variety of different forms of cellular stress can cause protein misfolding and aggregation and proteotoxicity. The cytoprotective response to proteotoxicity is termed the integrated stress response and involves 4 distinct serine/threonine protein kinases that converge on the translation initiation factor eIF2α, resu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: McConkey DJ

    更新日期:2017-01-15 00:00:00

  • Constitutive hypophosphorylation of extracellular signal-regulated kinases-1/2 and down-regulation of c-Jun in human gastric adenocarcinoma.

    abstract::Hyperphosphorylation of extracellular signal-regulated protein kinases-1/2 (ERK1/2) is known to promote cancer cell proliferation. We therefore investigated the constitutive phosphorylation levels of ERK1/2 and the expression of its downstream targets c-Fos, c-Jun, and cyclooxygenase-2 (COX-2) in biopsied human gastri...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wu WK,Sung JJ,Yu L,Li ZJ,Chu KM,Cho CH

    更新日期:2008-08-22 00:00:00

  • Nucleolar stress characterized by downregulation of nucleophosmin: a novel cause of neuronal degeneration.

    abstract::Nucleophosmin (NPM) is a multifunctional nucleolar protein that has been linked with nucleolar stress. In non-neuronal cell lines, NPM may enhance or inhibit the activity of tumor suppressor p53, a major apoptotic protein. The relationship between NPM and p53 in the central nervous system (CNS) remains unknown. Here, ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Marquez-Lona EM,Tan Z,Schreiber SS

    更新日期:2012-01-06 00:00:00

  • Cyclosporine A exhibits gender-specific nephrotoxicity in rats: Effect on renal tissue inflammation.

    abstract::Cyclosporine A (CSA) is a widely used immunosuppressant drug known to commonly cause cardio and nephrotoxicity. A study looking at the sex specificity of the cardiotoxicity of CSA revealed that sexual dimorphism existed when looking at the electrocardiographs and left ventricles of CSA-treated rats. We hypothesized th...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: El-Bassossy HM,Eid BG

    更新日期:2018-01-01 00:00:00

  • Duplicated cytoglobin genes in teleost fishes.

    abstract::Cytoglobin is a recently discovered myoglobin-related O2-binding protein of vertebrates with uncertain function. It occurs as single-copy gene in mammals. Here, we demonstrate the presence of two paralogous cytoglobin genes (Cygb-1 and Cygb-2) in the teleost fishes Danio rerio, Oryzias latipes, Tetraodon nigroviridis,...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fuchs C,Luckhardt A,Gerlach F,Burmester T,Hankeln T

    更新日期:2005-11-11 00:00:00

  • DNA methylation status influences insulin-induced glucose transport in 3T3-L1 adipocytes by mediating p53 expression.

    abstract::Researchers frequently use 3T3-L1 adipocytes as a fat cell line, but the capacity of this line for insulin-mediated glucose transport is lower than that of primary isolated fat cells. In this study, we found that 5-azacytidine (5-aza-C), DNA methyltransferase 1 inhibitor, enhanced insulin-stimulated 2-deoxyglucose (2-...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuroda M,Onoyama R,Sasaki W,Sebe M,Kitamura T,Masumoto S,Tsutsumi R,Harada N,Sakaue H

    更新日期:2020-02-16 00:00:00

  • Episodic positive selection during the evolution of naphthalene dioxygenase to nitroarene dioxygenase.

    abstract::Using different maximum-likelihood models of adaptive evolution, signatures of natural selective pressure, operating across the naphthalene family of dioxygenases, were examined. A lineage- and branch-site specific combined analysis revealed that purifying selection pressure dominated the evolutionary history of the e...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dutta A,Chakraborty J,Dutta TK

    更新日期:2013-10-11 00:00:00

  • Hormone-sensitive lipase is critical mediators of acute exercise-induced regulation of lipolysis in rat adipocytes.

    abstract::The purpose of the present study was to investigate the effect of acute exercise on lipolysis via coordination of hormone-sensitive lipase (HSL) and scaffold proteins, i.e., perilipin A and comparative gene identification-58 (CGI-58), in rat primary adipocytes. Glycerol release was significantly elevated immediately (...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ogasawara J,Nomura S,Rahman N,Sakurai T,Kizaki T,Izawa T,Ishida H,Haga S,Ohno H

    更新日期:2010-09-10 00:00:00

  • Conjugal transfer of plasmid and transposon DNA from Escherichia coli into Porphyromonas gingivalis.

    abstract::Using the broad host-range vector R751 to provide transfer functions, plasmid pVAL-1 and transposon Tn4351 were conjugally mobilized from Escherichia coli into Porphyromonas gingivalis. Transfer frequencies for both elements varied between 10(-6) and 10(-11), depending upon the recipient. The behavior of pVAL-1 and Tn...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dyer DW,Bilalis G,Michel JH,Malek R

    更新日期:1992-07-31 00:00:00